高敏感度銦鎵鋅氧化物薄膜電晶體 應用於EB病毒蛋白質之檢測

Abstract

在這篇論文中介紹以氧化銦鎵鋅薄膜電晶體與感測金屬板構成之生醫感測器檢測人類皰疹病毒第四型病毒蛋白質。此研究分兩部分： 在第一部分，我們首先利用延伸感測板之薄膜電晶體生醫感測器量測人類皰疹病毒第四型的核抗原抗體與早期抗原抗體。將量測結果與數種現行臨床檢測方法做比較以驗證薄膜電晶體生醫感測器的可靠性。省時、高敏感度是此感測器的顯著優勢。我們可以專一性量測到濃度為 10 -5 µg/ml 的 EBV 抗體溶液。 在第二部分，我們介紹改良型可重複使用之薄膜電晶體生醫感測器。分離原生 醫感測器之薄膜電晶體部分與感測板部分再以棒線方式聯結。他的優點在於只要在每次量測後更換感測板部分，薄膜電晶體部分可以被保存以達成重複使用之需求。在這部分，我們量測人類皰疹病毒第四型的核抗原抗體。並將薄膜電晶體生醫感測器與酵素免疫分析法之量測結果交叉比對，以驗證薄膜電晶體生醫感測器之可靠性。

In this thesis, a biosensor consists of an Indium-Gallium-Zinc-Oxide (IGZO) thin film transistor (TFT) biosensor and a gold sensing pad is demonstrated. There are two parts in this thesis. In the first part, a TFT biosensor with an extended gold sensing pad is demonstrated. EBV antibodies are measured and the results are compared with three conventional biological detection methods to ensure the reliability of the TFT biosensor. Time-saving and high sensitivity are the significant advantages. The TFT biosensor is able to selectively detect 10-5µg/ml EBV antibodies in the PBS buffer solution. In the second part, a modified reusable TFT biosensor is demonstrated. The TFT section and the sensing pad section of the biosensors are separated and then combined by wire bonding. The main advantage is that TFT transistors can be reused after each measurement by substituting the external gold sensing pads. A Cross-matching comparison of ELISA approach and TFT biosensor is discussed in order to verify reliability and credibly of the TFT biosensor.