請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/49395
標題: | 以能量觀點探討表皮生長因子受體之活化對粒線體增生以及癌細胞存活之影響 An energy point of view towards the effect of EGFR stimulation on mitochondria proliferation and cancer cell survival |
作者: | Hsing Kao 高興 |
指導教授: | 游偉絢 |
關鍵字: | 表皮生長因子受體,酪氨酸激?抑制劑,溶素,粒線體,非小型肺癌細胞,黃綠青黴素, EGFR,TKI,MMP7,mitochondria,NSCLC,citreoviridin, |
出版年 : | 2016 |
學位: | 碩士 |
摘要: | The over-expression of epidermal growth factor receptor (EGFR) has been observed in many cancer types including lung carcinoma. Since EGFR signaling significantly enhances cell growth and survival, several EGFR tyrosine kinase inhibitors (TKI) have been developed to specifically shut down EGFR signaling pathway. However, drug resistance occurs in most cases resulting from the secondary EGFR mutations. TKI-resistant EGFR mutation has been the major problem against TKI-based therapy. Since EGFR activation promotes tumor survival and proliferation, elevated energy supply must be achieved to meet such activities. Previous studies in our lab had found that the direct over-expression of MMP7 can induce mitochondria proliferation. To find the upstream regulator of MMP7, in this thesis we observed that the amount of MMP7 and mitochondria increased after EGF stimulation in NSCLC cell line CL1-0. Different EGFR mutants also possessed different mitochondria induction abilities, and the levels of MMP7 expression were also proportional to mitochondria content. Based on these findings, we treated these cell lines with the mitochondria ATP synthase inhibitor citreoviridin to see the cell viability differences. As expected, cells with higher mitochondria abundancy were more sensitive to citreoviridin, implying that the induction of mitochondria proliferation by EGFR may shift the cell metabolism status to be more mitochondria-dependent. In this study, we established a positive relationship between EGFR, MMP7 and mitochondria and also provided an energy point of view toward EGFR related cancer treatment. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/49395 |
DOI: | 10.6342/NTU201603199 |
全文授權: | 有償授權 |
顯示於系所單位: | 生物化學暨分子生物學科研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-105-1.pdf 目前未授權公開取用 | 1.52 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。