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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 吳瑞碧 | |
| dc.contributor.author | Wei-Ting Liu | en |
| dc.contributor.author | 劉威廷 | zh_TW |
| dc.date.accessioned | 2021-06-15T04:54:18Z | - |
| dc.date.available | 2013-08-05 | |
| dc.date.copyright | 2010-08-05 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-07-30 | |
| dc.identifier.citation | 呂習彣：不同溶液浸漬桑椹對花青素之萃取與萃取液呈色之影響，國立台灣大學園藝學研究所碩士論文，台北，台灣 (2006)。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46111 | - |
| dc.description.abstract | 本研究探討桑椹汁 (Mulberry juice) 花青素對於神經細胞的保護功效。研究結果顯示，冷凍乾燥後的桑椹汁粉末 (Mulberry juice powder, MJP) 含有約19 mg/g之花青素；經過固相萃取裝置純化後凍乾之桑椹汁花青素萃取物粉末 (Anthocyanin extract from ulberry juice, AEMJ) ，其總花青素含量高達243 mg/g。HPLC 分析結果則顯示桑椹汁中花青素組成主要為cyanindin-3-glucoside 以及 cyanidin-3-rutinoside。在體外 (in vitro) 抗氧化能力方面，MJP 濃度提高至 4 mg/ml 時，1,1-diphenyl-2-picrylhydrazyl (DPPH) 自由基清除能力可達約81%；花青素萃取物自由基清除能力與兒茶素相當，當濃度達到 1 mg/ml時，清除能力高於兒茶素，約為90%。MJP 的還原力隨著濃度升高，吸光值也提高，當濃度達2 mg/ml時，吸收強度約為1.58；AEMJ的還原力則與兒茶素相似，當濃度到達0.25 mg/ml時，吸收強度已達2左右。細胞實驗方面，以大鼠神經瘤 C6 細胞缺氧模式作為神經元細胞保護之評估模式。以5 mM Na2S2O4 處理誘導缺氧之控制組細胞存活率為60.98%；缺氧誘導前，共培養之 MJP濃度提高至25 μg anthocyanin/ml，缺氧模式 C6 細胞存活率為89.74%；共培養之AEMJ濃度達50 μg anthocyanin/ml時，缺氧模式 C6細胞存活率為92.21%。在細胞內源性抗氧化能力測試方面，隨著 AEMJ 處理濃度增加，細胞 glutathione 有上升的趨勢，但 superoxide dismutase則有減少的趨勢。實驗結果顯示 MJP 及 AEMJ 確實有保護缺氧模式 C6 細胞之功效。 | zh_TW |
| dc.description.abstract | This study investigated the cytoprotection effect of mulberry juice (MJ) anthothyanin on neuronal glial cell. The results showed that freeze dried mulberry juice powder (MJP) contained 19 mg /g anthocyanin. After purified with solid phase extraction device, anthocyanin content in freeze dried anthocyanin extract from mulberry juice (AEMJ) increased to 243 mg/g. The HPLC analysis revealed that cyanindin-3-glucoside and cyanidin-3-rutinoside are the major anthocyanin in AEMJ. The results of in vitro antioxidant test revealed that the DPPH free radical scavenging ability of MJP achieved to 81% at a concentration of 4 mg/ml. The DPPH free radical scavenging ability of 1 mg/ml AEMJ achieved to 90%, higher than that of catechin at the same concentration. The reducing power of MJP increased with the treated concentration. The absorption value was about 1.58 with the treated concentration of 2 mg/ml. The reducing power of AEMJ, similar to catechin, achieved to 2 at a concentration of 0.25 mg/ml. The rat glial tumor C6 cell was used for investigating the cytoprotection ability of MJP or AEMJ under hypoxia condition induced with Na2S2O4. The cell viability was 60.98 % for hypoxia rat glial tumor C6 cell control group at Na2S2O4 concentration of 5 mM. The cell viability of hypoxia C6 cells was 89.74% when pre-incubated with MJP contains 25 μg /ml anthocyanin. The cell viability of hypoxia C6 cells was 92.21% when pre-incubated with AEMJ contains 50 μg /ml anthocyanin. The intercellular antioxidant glutathione of hypoxia C6 cells increased, however, the superoxide dismutase activity of hypoxia C6 cells decreased, when pre-cultured with AEMJ. The results indicated that both MJP and AEMJ possess cytoprotection ability on hypoxia rat C6 glial cells. | en |
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| dc.description.tableofcontents | 摘 要...................................................I
Abstract.................................................II 目 錄...................................................III 圖 次...................................................V 表 次...................................................VI 第一章 緒論.............................................1 第二章 文獻整理..........................................2 第一節 老化及腦部功能退化...............................2 第二節 花青素...........................................3 (1) 在植物中之功用.......................................3 (2) 化學特性及結構.......................................3 (3) 生理活性及抗氧化功能.................................4 第三節 桑椹萃取物之生理活性分析..........................6 第四節 大鼠神經膠瘤細胞 (C6) 保護模式....................7 第五節 細胞內源性抗氧化物質..............................7 第三章 實驗架構及方法....................................14 第一節 實驗架構..........................................14 第二節 實驗方法..........................................14 (1) 桑椹汁製備...........................................14 (2) 花青素之分離與純化...................................15 (3) HPLC 分析............................................15 (4) 清除DPPH 自由基能力測定..............................15 (5) 還原力測定...........................................16 (6) 總花青素酸鹼度差額法測定.............................16 (7) 細胞實驗.............................................17 (8) Glutathione (GSH) 濃度之測定.........................18 (9) Superoxide dismutase (SOD) 濃度之測定................19 (10) 統計分析............................................20 第三節 實驗藥品及器材...................................20 第四章 實驗結果與討論...................................30 第一節 MJP及 AEMJ花青素含量檢定.........................30 第二節 MJP及 AEMJ抗氧化能力檢定.........................30 第三節 MJP 及 AEMJ 抗氧化能力檢定.......................30 (1) DPPH自由基清除能力...................................31 (2) 還原力測定...........................................31 第四節 細胞實驗.........................................32 (1) 細胞毒性測試.........................................32 (2) 細胞缺氧模式下之保護作用.............................32 第五節 細胞內源性抗氧化物質..............................33 (1) GSH 含量測定.........................................33 (2) SOD 活性測定.........................................34 第五章 結論.............................................43 參考文獻.................................................44 | |
| dc.language.iso | zh-TW | |
| dc.title | 桑椹汁花青素抗氧化能力及對大鼠 C6 神經細胞保護功效之探討 | zh_TW |
| dc.title | Antioxidant activity and rat C6 nerve cell cytoprotection ability of anthocyanins from mulberry juice | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 王進崑,林士祥,沈賜川,馬嘉軉 | |
| dc.subject.keyword | 桑椹汁,花青素,細胞缺氧模式,C6 神經細胞,抗氧化,細胞保護, | zh_TW |
| dc.subject.keyword | mulberry juice,anthocyanin,hypoxia model,C6 cells,antioxidant,cytoprotection, | en |
| dc.relation.page | 53 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-07-30 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 食品科技研究所 | zh_TW |
| 顯示於系所單位： | 食品科技研究所 | |
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| ntu-99-1.pdf 目前未授權公開取用 | 725.52 kB | Adobe PDF |
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