利用奈米粒子布朗運動生醫感測技術於 心血管疾病危險因子之檢測

Abstract

血清液體黏性及C反應蛋白濃度為心血管疾病危險因子之ㄧ。在本篇研究中，利用奈米粒子布朗運動的速度變化，做為生醫感測技術的檢測方式，並以此感測技術檢測血清液體黏性及C反應蛋白濃度。布朗運動的量測是以微粒子追蹤測速儀與全反射螢光顯微鏡技術做為量測工具。實驗結果得知，當液體黏性每增加1cP，布朗運動速度約減慢10%左右；在CRP與奈米粒子結合過程中，受到粒子平均直徑增加，使得布朗運動速度隨著反應時間增加而減慢。因此CRP濃度越高，布朗運動速度越慢。未來可將此感測技術應用於其他生物粒子檢測，以及實際心血管疾病危險因子臨床檢測上。

Both the viscosity and the C-reactive protein (CRP) concentration of the serum are the symptoms of risk factors for cardiovascular diseases (CVD). In this study, a bio-sensing technique was employed to detect the viscosity and the CRP concentration of the serum by measuring nanobeads’ Brownian motion. Micro-Particle-Tracking-Velocimetry (μ-PTV) technique and Total Internal Reflection Fluorescence (TIRF) technique were used to obtain the characteristics of nanobeads’ Brownian diffusion. The results showed that as the viscosity increases 1cP, the nanobeads’ Brownian velocity was found to decrease about 10%. During the binding process of CRP onto nanobeads, the mean value of the beads’ diameters increases so that the Brownian velocity decreases with an increase of time. Moreover, higher CRP concentration leads to a lower Brownian velocity of the nanobeads in the equilibrium state. In the future, this simple sensing technique can be used to detect other bio-molecules and applied to the clinical assay of cardiovascular diseases risk factors.