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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 蔡進發(Jing-Fa Tsai) | |
dc.contributor.author | Yi-Hsing Liu | en |
dc.contributor.author | 劉宜鑫 | zh_TW |
dc.date.accessioned | 2021-06-15T02:53:13Z | - |
dc.date.available | 2014-08-12 | |
dc.date.copyright | 2009-08-12 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-08-04 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44362 | - |
dc.description.abstract | 血清液體黏性及C反應蛋白濃度為心血管疾病危險因子之ㄧ。在本篇研究中,利用奈米粒子布朗運動的速度變化,做為生醫感測技術的檢測方式,並以此感測技術檢測血清液體黏性及C反應蛋白濃度。布朗運動的量測是以微粒子追蹤測速儀與全反射螢光顯微鏡技術做為量測工具。實驗結果得知,當液體黏性每增加1cP,布朗運動速度約減慢10%左右;在CRP與奈米粒子結合過程中,受到粒子平均直徑增加,使得布朗運動速度隨著反應時間增加而減慢。因此CRP濃度越高,布朗運動速度越慢。未來可將此感測技術應用於其他生物粒子檢測,以及實際心血管疾病危險因子臨床檢測上。 | zh_TW |
dc.description.abstract | Both the viscosity and the C-reactive protein (CRP) concentration of the serum are the symptoms of risk factors for cardiovascular diseases (CVD). In this study, a bio-sensing technique was employed to detect the viscosity and the CRP concentration of the serum by measuring nanobeads’ Brownian motion. Micro-Particle-Tracking-Velocimetry (μ-PTV) technique and Total Internal Reflection Fluorescence (TIRF) technique were used to obtain the characteristics of nanobeads’ Brownian diffusion. The results showed that as the viscosity increases 1cP, the nanobeads’ Brownian velocity was found to decrease about 10%. During the binding process of CRP onto nanobeads, the mean value of the beads’ diameters increases so that the Brownian velocity decreases with an increase of time. Moreover, higher CRP concentration leads to a lower Brownian velocity of the nanobeads in the equilibrium state. In the future, this simple sensing technique can be used to detect other bio-molecules and applied to the clinical assay of cardiovascular diseases risk factors. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T02:53:13Z (GMT). No. of bitstreams: 1 ntu-98-R96543017-1.pdf: 3644469 bytes, checksum: 68215739068315a85ffa6b666f3a6933 (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | 中文摘要 I
Abstract II 目錄 III 表目錄 VI 圖目錄 VII 符號目錄 X 第一章 緒論 1 1-1 前言 1 1-2 研究動機及目的 2 1-3 文獻回顧 3 1-3-1 布朗運動實驗文獻回顧 3 1-3-2 C反應蛋白(C-reactive protein)的簡介 5 1-4 研究方法 8 1-5 論文架構 8 第二章 布朗運動 10 2-1 布朗運動數學模式 10 2-2 布朗運動與邊界效應影響 13 2-3 生物分子間之親和力作用 15 第三章 TIRF及PTV 介紹 17 3-1 TIRF原理 17 3-1-1 簡述 17 3-1-2 Snell’s Law 17 3-1-3 穿透深度 18 3-1-4 訊號背景比 19 3-2微粒子顯像�追蹤測速儀原理 20 第四章 實驗設備架構原理與實驗步驟 22 4-1 實驗設備 22 4-2 微流道製作 24 4-2-1 SU-8母模製作 25 4-2-2 翻模接合製程 26 4-3 抗體的自組裝於奈米懸浮粒子表面實驗 27 4-4 甘油-水混合液 29 第五章 實驗結果與討論 31 5-1 利用μ-PTV做布朗運動研究之分析 31 5-2 布朗運動與邊界效應之研究 33 5-3 兩段式檢測法(Two-steps detection)說明 35 5-4 液體黏性檢測實驗 36 5-5 抗原抗體辨識實驗 39 第六章 結論與未來展望 42 6-1 結論 42 6-2 未來展望 43 參考文獻 44 附錄 78 | |
dc.language.iso | zh-TW | |
dc.title | 利用奈米粒子布朗運動生醫感測技術於
心血管疾病危險因子之檢測 | zh_TW |
dc.title | Measurements of Nanobead’s Brownian Motion for the Detection of Cardiovascular Diseases Risk Factors | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 沈弘俊(Horn-Jiunn Sheen) | |
dc.contributor.oralexamcommittee | 吳光鐘(Kuang-Chong Wu),林世明(Shi-Min Lin),黃榮山(Long-Sun Huang) | |
dc.subject.keyword | 布朗運動,C反應蛋白,心血管疾病,微粒子追蹤測速儀,全反射螢光顯微鏡, | zh_TW |
dc.subject.keyword | Brownian motion,C-Reactive Protein (CRP),Cardiovascular Diseases (CVD),Micro-Particle-Tracking-Velocimetry (PTV),Total Internal Reflection Fluorescence (TIRF), | en |
dc.relation.page | 79 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2009-08-04 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 工程科學及海洋工程學研究所 | zh_TW |
顯示於系所單位: | 工程科學及海洋工程學系 |
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