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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43642完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 蔣丙煌(Been-Huang Chiang) | |
| dc.contributor.author | Ya-Wen Hou | en |
| dc.contributor.author | 侯雅雯 | zh_TW |
| dc.date.accessioned | 2021-06-15T02:24:59Z | - |
| dc.date.available | 2016-08-20 | |
| dc.date.copyright | 2011-08-20 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-16 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43642 | - |
| dc.description.abstract | 本實驗室先前之研究顯示，仙鶴草的乙醇萃取物具有皮膚保健功效，而根據文獻，清酒也有使保護皮膚免於水分散失之功能。因此，本研究乃試著結合清酒及仙鶴草對皮膚保健的益處，期望能研發一發酵產物，具有皮膚保健之功效。本研究第一部分以不同濃度乙醇 (10%~95%) 萃取仙鶴草，發現 40% 乙醇萃取物有最佳之抑制基質金屬蛋白酶活性，但乙醇萃取物並無促進皮膚纖維母細胞產生 Type I procollagen 的能力，且在 500 和 1000 ug/ml濃度下對皮膚細胞有毒性。第二部分將仙鶴草添加於蒸飯中一起發酵，發酵得到的產物可分成先添加仙鶴草組和後添加仙鶴草組，並與無添加仙鶴草之清酒組比較。結果顯現，發酵產物雖然對基質金屬蛋白酶的抑制活性較低，但其對皮膚細胞不具毒性，且在濃度為 50 ug/ml 下，後添加組、先添加組和清酒組皆可促進皮膚纖維母細胞 Type I procollagen 之生成，與控制組相比，增生率分別為 17%、 30%、 34%。在清除因UV誘導皮膚產生 ROS 能力方面，乙醇萃取物無清除 ROS 之能力。在發酵樣品中，不論是先添加與後添加仙鶴草組別，皆可降低皮膚細胞內 ROS 的生成，尤其以清酒組具有顯著性降低因 UV 誘導皮膚纖維母細胞 (CCD-966SK) 產生之 ROS 之功效。綜言之，以仙鶴草添加於清酒發酵所得的發酵產物具有降低 UV 誘導產生 ROS 之能力，可促進皮膚纖維母細胞產生 Type I procollagen，並對細胞不具毒性，期望可應用於發展皮膚抗老化之產品。 | zh_TW |
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| dc.description.tableofcontents | 中文摘要 ...................................................I
Abstract..................................................II 圖次....................................................VIII 表次.......................................................X 第一章 前言...............................................1 第二章 文獻回顧............................................3 一、 人類皮膚............................................3 (一) 人類皮膚的結構與功能..................................3 1. 表皮層..............................................4 2. 真皮層..............................................5 3. 皮下組織層..........................................5 (二) 皮膚老化............................................6 1. 皮膚之老化..........................................6 2. 皮膚老化的機制.......................................6 3. 皮膚老化的調控.......................................9 二、 仙鶴草.............................................11 (一) 仙鶴草的分類及用途...................................11 (二) 仙鶴草的成分........................................12 (三) 仙鶴草的生理活性.....................................13 1. 抗氧化活性.........................................13 2. 抗高血糖之活性......................................13 3. 抑制人類胃癌細胞 MGC-803之生長........................13 4. 抑制發炎反應........................................13 (四) 仙鶴草與皮膚保健相關的活性成分.........................14 1. Tannin............................................14 2. Luteolin..........................................14 3. Quercetin.........................................14 4. Catechin..........................................14 三、 清酒..............................................16 (一) SK-II之靈感........................................16 (二) 清酒與皮膚之相關研究.................................16 第三章 實驗目的與架構......................................19 一、研究目的................................................19 二、實驗架構................................................20 (一) 以不同酒精濃度萃取仙鶴草，篩選出具皮膚保健功效 (tyrosinase, MMPs) 的最佳酒精濃度萃取物...................................20 (二) 在清酒的酒醪 (sake mash) 中加入仙鶴草進行發酵，希望清酒產生酒精之際能將仙鶴草有效成分萃取出來，並比較清酒組和仙鶴草組之皮膚保健功效 ..........................................................21 1. 製米麴 (koji rice)............................... ..21 2. 製備酒母 (moto)....................................22 3. 於酒醪 (sake mash) 中添加仙鶴草進行發酵並進行比較皮膚保健之評估 .................................................23 第四章 材料與方法............................................24 一、實驗材料................................................24 (一) 實驗原料..............................................24 (二) 實驗菌種..............................................24 (三) 實驗細胞株.............................................24 (四) 實驗藥品與試劑..........................................24 二、實驗方法................................................26 (一) 菌種活化及保存..........................................26 1. Aspergillus oryzae....................................26 2. Saccharomyces cerevisiae..............................27 (二) 仙鶴草添加於清酒釀製之架構圖..............................28 1. 製麴...................................................29 2. 酒母之製備..............................................29 3. 酒醪之製備..............................................29 (三) 酵素活性抑制能力分析.....................................31 1. 膠原蛋白分解酶活性抑制分析.................................31 2. 彈力蛋白分解酶活性抑制分析.................................31 3. 明膠蛋白分解酶活性抑制分析.................................31 4. 酪胺酸酶活性抑制測定......................................32 (四) 細胞實驗..............................................33 1. 樣品之製備..............................................33 2. 細胞株.............................................33 3. 細胞繼代培養........................................33 4. 細胞保存...........................................33 5. 細胞計數方式........................................34 6. 細胞增生與毒性試驗...................................34 7. 第Ι型原膠原蛋白 (type Ι procollagen, PIP ) 含量分析...35 8. 清除模擬太陽光光源誘導纖維母細胞內產生ROS之能力...........35 (五) 成分分析..............................................37 1. 清酒中alpha-EG的含量測定.................................37 2. 仙鶴草與皮膚保健有關的成分分析..............................38 3. 總酚含量之測定......................................39 4. 類黃酮含量之測定.....................................39 (六) 統計分析..............................................39 第五章 結果與討論.........................................40 一、 樣品之製備及成分分析.................................40 (一) 樣品之製備.........................................40 (二) 樣品之成分分析......................................40 1. alpha-EG之含量.....................................40 2. Quercetin之含量....................................41 3. Ellagic acid 之含量................................41 4. 總多酚和類黃酮之含量.................................42 二、皮膚保健功效評估.........................................44 (一) 抑制酪胺酸酶 (tyrosinase) 的活性.......................44 (二) 分解皮膚基質的蛋白酶活性抑制實驗...........................45 1. 膠原蛋白分解酶活性抑制................................46 2. 明膠蛋白分解酶活性抑制................................47 3. 彈力蛋白分解酶活性抑制................................47 (三) 皮膚纖維母細胞 (CCD-966SK) 的毒性試驗.................48 (四) 促進人類纖維母細胞株生成 type Ι procollagen...........49 (五) 清除細胞內活性氧物質 (ROS) 之能力.....................50 1. 清除細胞內活性氧物質之能力............................50 2. 利用UV照射器誘導....................................50 第六章 結論..............................................52 第七章 未來展望...........................................55 第八章 參考文獻...........................................81 附錄—小論文................................................90 | |
| dc.language.iso | zh-TW | |
| dc.title | 仙鶴草發酵產物之皮膚保健功效 | zh_TW |
| dc.title | Anti-aging effect of Agrimonia pilosa fermented product on skin | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 潘子明(PAN TZU-MING),吳瑞碧 | |
| dc.subject.keyword | 清酒,仙鶴草,發酵,纖維母細胞,基質金屬蛋白酶,活性氧物質,第一型原膠原蛋白, | zh_TW |
| dc.subject.keyword | Agrimonia pilosa,rice wine (sake),matrix metalloproteinase (MMPs),reactive oxygen species (ROS),type I procollagen, | en |
| dc.relation.page | 106 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-08-17 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 食品科技研究所 | zh_TW |
| 顯示於系所單位： | 食品科技研究所 | |
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