請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43125
標題: | 削弱LPA1功能抑制傷口癒合過程中嗜中性球之聚集 Knockdown of LPA1 inhibits neutrophil recruitment during wound healing |
作者: | Meng-Chieh Tsao 曹孟傑 |
指導教授: | 李士傑 |
關鍵字: | 水解磷脂酸接受器1,嗜中性球,發炎反應,斑馬魚, Lysophosphatidic acid receptor 1,neutrophil,inflammation,zebrafish, |
出版年 : | 2009 |
學位: | 碩士 |
摘要: | We have previously demonstrated the necessity of a lysophosphatidic acid (LPA) receptor, LPA1, in zebrafish lymphangiogenesis. One of the major functions of lymphatic system is to exert inflammatory response. Therefore, we examined the possible role of LPA1 in zebrafish imflammation. Here we performed a live-imaging analysis of neutrophil recruitment by using a transgenic zebrafish (Tg(MPO:EGFP)114), which expressed EGFP in neutrophils. The lpa1 morpholino (MO) caused a dose-dependent reduction in neutrophil recruitment to the wound site and this defect could be rescued completely by overexpression of lpa1. Flow cytometry analysis reveals that no significant change in the total number of neutrophil in the lpa1 morphants. Furthermore, the expressions of myelopoiesis-related genes, including mpx, lcp1 and spi1 were not affected by the lpa1 knockdown, either. These results demonstrated that the lpa1 MO exerted specific inhibition on neutrophilic inflammation. In addition, we provided evidence that knockdown of LPA1 has no significant effect on tail fin regeneration. Thus, we conclude that LPA1 specifically mediates neutrophilic inflammatory response in zebrafish. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43125 |
全文授權: | 有償授權 |
顯示於系所單位: | 動物學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-98-1.pdf 目前未授權公開取用 | 1.18 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。