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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李士傑 | |
| dc.contributor.author | Meng-Chieh Tsao | en |
| dc.contributor.author | 曹孟傑 | zh_TW |
| dc.date.accessioned | 2021-06-15T01:38:12Z | - |
| dc.date.available | 2011-07-17 | |
| dc.date.copyright | 2009-07-17 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-07-16 | |
| dc.identifier.citation | An, S., et al., 1998. Characterization of a novel subtype of human G protein-coupled receptor for lysophosphatidic acid. J Biol Chem. 273, 7906-10.
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The impact of cyclooxygenase-2 mediated inflammation on scarless fetal wound healing. Am J Pathol. 165, 753-61. Yoshida, K., et al., 2003. Vascular remodeling induced by naturally occurring unsaturated lysophosphatidic acid in vivo. Circulation. 108, 1746-52. Zarbock, A., Ley, K., 2009. Neutrophil adhesion and activation under flow. Microcirculation. 16, 31-42. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43125 | - |
| dc.description.abstract | We have previously demonstrated the necessity of a lysophosphatidic acid (LPA) receptor, LPA1, in zebrafish lymphangiogenesis. One of the major functions of lymphatic system is to exert inflammatory response. Therefore, we examined the possible role of LPA1 in zebrafish imflammation. Here we performed a live-imaging analysis of neutrophil recruitment by using a transgenic zebrafish (Tg(MPO:EGFP)114), which expressed EGFP in neutrophils. The lpa1 morpholino (MO) caused a dose-dependent reduction in neutrophil recruitment to the wound site and this defect could be rescued completely by overexpression of lpa1. Flow cytometry analysis reveals that no significant change in the total number of neutrophil in the lpa1 morphants. Furthermore, the expressions of myelopoiesis-related genes, including mpx, lcp1 and spi1 were not affected by the lpa1 knockdown, either. These results demonstrated that the lpa1 MO exerted specific inhibition on neutrophilic inflammation. In addition, we provided evidence that knockdown of LPA1 has no significant effect on tail fin regeneration. Thus, we conclude that LPA1 specifically mediates neutrophilic inflammatory response in zebrafish. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T01:38:12Z (GMT). No. of bitstreams: 1 ntu-98-R96b41002-1.pdf: 1213257 bytes, checksum: dbe9609912b2b680264788ee16f0e4e3 (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 中文摘要 i
Abstract ii Contents iii List of Figures iv Introduction 1 Materials and Methods 5 Zebrafish maintenance and embryo collection 5 Morpholino oligonucleotide (MO) knockdown and Gene overexpression 5 Microinjection 6 Live-imaging analysis of neutrophil recruitment 6 Whole-mount in situ hybridization 7 Flow cytometry of zebrafish larvae 8 Tail fin regeneration analysis 8 Gene cloning and analysis of zebrafish il-8 8 Statistical analysis 9 Results 10 Knockdown of LPA1 reduces neutrophil recruitment in a dose-dependent manner 10 Overexpression of zebrafish LPA1 rescues the inhibition on neutrophil recruitment 11 The total number of neutrophil is not reduced in the lpa1 morphants 12 No significant changes in expressions of myelopoiesis-related genes in the lpa1 morphant 13 Inhibition of LPA1 does not alter the tail fin regeneration speed 13 Zebrafish interleukin-8 (il-8) expresses at the wound site 14 Discussion 15 References 18 Figures 26 | |
| dc.language.iso | en | |
| dc.subject | 斑馬魚 | zh_TW |
| dc.subject | 水解磷脂酸接受器1 | zh_TW |
| dc.subject | 嗜中性球 | zh_TW |
| dc.subject | 發炎反應 | zh_TW |
| dc.subject | Lysophosphatidic acid receptor 1 | en |
| dc.subject | zebrafish | en |
| dc.subject | inflammation | en |
| dc.subject | neutrophil | en |
| dc.title | 削弱LPA1功能抑制傷口癒合過程中嗜中性球之聚集 | zh_TW |
| dc.title | Knockdown of LPA1 inhibits neutrophil recruitment during wound healing | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 鐘邦柱,劉薏雯 | |
| dc.subject.keyword | 水解磷脂酸接受器1,嗜中性球,發炎反應,斑馬魚, | zh_TW |
| dc.subject.keyword | Lysophosphatidic acid receptor 1,neutrophil,inflammation,zebrafish, | en |
| dc.relation.page | 34 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2009-07-16 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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