藉由高效液相層析法之新一代管柱－整體式管柱分析番瀉錠劑中之番瀉葉苷A與番瀉葉苷B

Abstract

番瀉錠劑中最主要且含量最多的活性成分為sennoside A及sennoside B,臨床上常用來治療便秘,但服用過量會造成腸絞痛及嚴重的腹瀉,為了能在正常劑量服用下達到有效的醫療用途,確知番瀉錠劑中sennoside A及sennoside B的含量是必須的,同時為了能快速且有效的於番瀉錠劑中測定sennoside A及sennoside B的含量,本研究使用了整體式管柱(monolithic column)類型之一的ChromolithTM performance RP-18e當管柱,此管柱相較於傳統使用的粒子充填試管柱(particle-packed column)具有較低的管柱內壓,故可在高流速下進行分析以減少分離的時間。 在分析方法建立的過程中,探討緩衝溶液濃度、緩衝溶液pH值、離子對試劑的濃度、有機溶媒修飾劑體積、管柱溫度及移動相流速等影響分離的參數,最後找到一個最適化的分離條件為―管柱: ChromolithTM performance RP-18e (100

Sennoside A and sennoside B are the most significant and abundant active components in senna tablets. Clinically, they are used for constipation treatment. However, overdose will cause tormina and severe diarrhea. In order to make sure and determine the doses of sennoside A and sennoside B effectively, this study employed one of the monolithic columns, ChromolithTM performance RP-18e. Compared to the traditional particle-packed column, this one is with lower column pressure and thus it can be analyzed under high flow rate to reduce separation time. In the course of method development, parameters including concentration and pH value of buffer solution, concentration of ion-pair reagents, volume of organic solvent modifier, column temperature and flow rate of the mobile phase were varied to find out the optimum conditions of separation. The most optimum conditions were : ChromolithTM performance RP-18e (100