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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/39387完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 孫紹文 | |
| dc.contributor.author | Chia-Yun Wen | en |
| dc.contributor.author | 溫嘉筠 | zh_TW |
| dc.date.accessioned | 2021-06-13T17:27:21Z | - |
| dc.date.available | 2005-01-05 | |
| dc.date.copyright | 2005-01-05 | |
| dc.date.issued | 2004 | |
| dc.date.submitted | 2004-12-27 | |
| dc.identifier.citation | 1. 現代本草中國藥材學 (上), 啟業書局, 台北, 台灣, 1987, 29頁
2. W. C. Evans, Trease and Evan’s pharmacognosy, 14th ed., WB Saunders, London, UK, 1996, pp. 233-239. 3. 現代本草中國藥材學 (下), 啟業書局, 台北, 台灣, 1987, 714頁 4. 王仁澤,王健行及王利伶,食品毒性學,高立圖書有限公司, 台北, 台灣, 1996, 322頁 5. P. M. Dewick, Medicinal Natural Products, A Biosynthetic Approach, 2nd ed., John Wiley & Sons, Chichester, UK, 2002, pp. 63-70. 6. AHFS Drug Information, American Society of Health-System Pharmacists Inc., MD, USA, 2003, pp. 2747-2748. 7. The Merck index, 13th ed., Merck & Co., Whitehouse Station, NJ, USA, 2001, p.1516. 8. United states Pharmacopeia, 24th ed., United States Pharmacopeial Convention, Twinbrook Parkway, Rockville, MD, USA, 2003, pp. 1677-1679. 9. 中華藥典, 第五版, 衛生署中華藥典編修委原會, 中華民國89年, 1139-1140頁. 10. European Pharmacopoeia, 3rd ed., European Department for the Quality of Medicines, Council of Europe, Strasbourg, 1997, pp.1459-1464. 11. British Pharmacopoeia, Stationery Office, Department of Health, London, UK, 2001, PP. 2376-2378. 12. 楊春茂, 林麗慧, 陳景宗, 余淑美, 林光輝及郭詩憲, 藥理學, 匯華圖書出版有限公司, 台北, 台灣, 1999, pp. 281-282. 13. WHO monographs on selected medicinal plants, World Health Organization, Geneva, Switzerland, 1999, pp. 241-258. 14. J. Westendorf, H. Marguardt, B. Poginsky, M. Dominiak, J. Schmidt and H. Marquardt, Genotoxicity of naturally occurring hydroxyanthraquinones, Mutat. Res., 240 (1990) 1-12. 15. J. F. Riemann, H. Schmidt and W. zimmermann, The fine structure of colonic submucosal nerves in patients with chronic laxative abuse, Scand. J. Gastroent, 15 (1980) 761-768. 16. D. Sandnes, T. Johansen, G. Teien and G. Ulsaker, Mutagenicity of crude senna and senna glycosides in Salmonella typhimurium, Pharmacology & Toxicology, 71 (1992) 165-172. 17. 蘇秀婷, 以HPLC法定量番瀉葉製劑中主成分番瀉苷A與B之研究, 國立台灣大學醫學院藥學研究所碩士論文, 2001, pp. 1-3. 18. H. Minakuchi, K. Nakanishi, N. Soga, N. Ishizuka and N. Tanaka, Effect of skeleton size on the performance of octadecylsilylated continuous porous silica columns in reversed-phase liquid chromatography, J. Chromatogr. A, 762 (1997) 135-146. 19. N. Tanaka, H. Kobayashi, K. Nakanishi, H. Minakuchi and N. Ishizuka, A new type of chromatographic support could lead to higher separation efficiencies, Anal. Chem., 73 (2001) 421-429A. 20. R. E. Majors, Advances in HPLC column packing design, LC. GC. Eur., 16 (2003), pp. 8-13. 21. H. Zou, X. Huang, M. Ye and Q. Luo, Monolithic stationary phases for liquid chromatography and capillary electrochromatography, J. Chromatogr. A, 954 (2002) 5-32. 22. M. Motokawa, H. Kobayashi, N. Ishizuka, H. Minakuchi, K. Nakanishi, H. Jinnai, K. Hosoya, T. Ikegami and N. Tanaka, Monolithic silica columns with various skeleton sizes and through-pore sizes for capillary liquid chromatography, J. Chromatogr. A, 961 (2002) 53-63. 23. N. Tanaka, H. Kobayashi, N. Ishizuka, H. Minakuchi, K. Nakanishi, K. Hosoya and T. Ikegami, Monolithic silica columns for high-efficiency chromatographic separations, J. Chromatogr. A, 965 (2002) 35-49. 24. J. E. MacNair, K. C. Lewis and J. W. Jorgenson, Ultrahigh-pressure reversed-phase liquid chromatography in a packed capillary column, Anal. Chem., 69 (1997) 983-989. 25. J. E. MacNair, K. D. Patel and J. W. Jorgenson, Ultrahigh-pressure reversed-phase capillary liquid chromatography: isocratic and gradient elution using columns packed with 1.0- | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/39387 | - |
| dc.description.abstract | 番瀉錠劑中最主要且含量最多的活性成分為sennoside A及sennoside B,臨床上常用來治療便秘,但服用過量會造成腸絞痛及嚴重的腹瀉,為了能在正常劑量服用下達到有效的醫療用途,確知番瀉錠劑中sennoside A及sennoside B的含量是必須的,同時為了能快速且有效的於番瀉錠劑中測定sennoside A及sennoside B的含量,本研究使用了整體式管柱(monolithic column)類型之一的ChromolithTM performance RP-18e當管柱,此管柱相較於傳統使用的粒子充填試管柱(particle-packed column)具有較低的管柱內壓,故可在高流速下進行分析以減少分離的時間。
在分析方法建立的過程中,探討緩衝溶液濃度、緩衝溶液pH值、離子對試劑的濃度、有機溶媒修飾劑體積、管柱溫度及移動相流速等影響分離的參數,最後找到一個最適化的分離條件為―管柱: ChromolithTM performance RP-18e (100 | zh_TW |
| dc.description.abstract | Sennoside A and sennoside B are the most significant and abundant active components in senna tablets. Clinically, they are used for constipation treatment. However, overdose will cause tormina and severe diarrhea. In order to make sure and determine the doses of sennoside A and sennoside B effectively, this study employed one of the monolithic columns, ChromolithTM performance RP-18e. Compared to the traditional particle-packed column, this one is with lower column pressure and thus it can be analyzed under high flow rate to reduce separation time.
In the course of method development, parameters including concentration and pH value of buffer solution, concentration of ion-pair reagents, volume of organic solvent modifier, column temperature and flow rate of the mobile phase were varied to find out the optimum conditions of separation. The most optimum conditions were : ChromolithTM performance RP-18e (100 | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T17:27:21Z (GMT). No. of bitstreams: 1 ntu-93-R91423017-1.pdf: 4218577 bytes, checksum: 55bf6bd1eed93bbc66b97f77047cbe3b (MD5) Previous issue date: 2004 | en |
| dc.description.tableofcontents | 目錄
壹、緒論................................................1 1-1蒽醌(Anthraquinones)之簡介………………………………1 1-1-1番瀉葉苷(sennoside)A及B……………………………2 1-1-2番瀉葉苷(sennoside)A及B之製劑………………… 3 1-1-3番瀉葉苷(sennoside)A及B之藥理作用機制及臨床應…3 1-2研究目的…………………………………………………………4 1-2-1確切測知蒽醌類配醣體的含量…………………………4 1-2-2縮短分離時間…………………………………………… 4 1-3高效液相層析法(high performance liquid chromatography)之簡介………………………………………………5 1-3-1粒子充填式管柱(particle-packed column)的限制…6 1-3-2改善粒子充填式管柱(particle-packed column)之限 制……………………………………………………………………… 8 1-3-2-1超高壓液相層析(ultrahigh-pressure liquid chromatography, UHPLC8)…………………………………………… 8 1-3-2-2毛細管電層析(capillary electrochromatography, CEC)…………………………………… 8 1-3-2-3開放式液相層析(open- tube liquid chromatography, OTLC)…………………………………………… 9 1-3-2-4整體式管柱(monolithic column)……………… 9 貳、研究方法與步驟...................................... 15 2-1分析方法之建立……………………………………………… 15 2-1-1緩衝溶液濃度………………………………………… 15 2-1-2緩衝溶液pH值…………………………………………16 2-1-3離子對試劑的濃度…………………………………… 16 2-1-4有機溶媒修飾劑體積………………………………… 17 2-1-5管柱溫度…………………………………………………17 2-1-6移動相流速………………………………………………17 2-2分析方法之確效………………………………………………18 2-2-1專一性 (specificity)…………………………………18 2-2-2精密度 (precision)………………………………… 18 2-2-3線性 (linearity)…………………………………… 18 2-2-4準確度 (accuracy)…………………………………… 19 2-2-5檢測極限 (limit of detection)……………………19 2-2-6耐變性(robustness)………………………………… 19 2-3錠劑中sennoside A及sennoside B含量的測定…………… 24 參、實驗部分………………………………………………………… 25 3-1儀器……………………………………………………………25 3-1-1高效液相層析(HPLC)儀………………………………25 3-1-2層析管柱………………………………………………25 3-1-3酸鹼度計………………………………………………25 3-2試藥及材料……………………………………………………25 3-3標準品…………………………………………………………26 3-4標準品溶液…………………………………………………… 26 3-5分析藥物………………………………………………………26 3-6分析方法發展過程中所使用之工作溶液................27 3-7檢品溶液………………………………………………………27 3-8 HPLC分析條件……………………………………………… 27 肆、結果與討論……………………………………………………… 28 4-1分析方法的建立 | |
| dc.language.iso | zh-TW | |
| dc.subject | 粒子充填式管柱 | zh_TW |
| dc.subject | 番瀉葉苷 | zh_TW |
| dc.subject | 整體式管柱 | zh_TW |
| dc.subject | monolithic column | en |
| dc.subject | sennoside | en |
| dc.subject | particle-packed column | en |
| dc.title | 藉由高效液相層析法之新一代管柱－整體式管柱分析番瀉錠劑中之番瀉葉苷A與番瀉葉苷B | zh_TW |
| dc.title | The use of a new generation column of high performance liquid chromatography, monolithic column, to analyze sennoside A and sennoside B in a senna tablet | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 忻凌偉,陳繼明 | |
| dc.subject.keyword | 粒子充填式管柱,整體式管柱,番瀉葉苷, | zh_TW |
| dc.subject.keyword | sennoside,monolithic column,particle-packed column, | en |
| dc.relation.page | 93 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2004-12-28 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 藥學研究所 | zh_TW |
| 顯示於系所單位： | 藥學系 | |
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