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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/3796完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 劉懷勝 | |
| dc.contributor.author | Yao-Chang Yang | en |
| dc.contributor.author | 楊曜彰 | zh_TW |
| dc.date.accessioned | 2021-05-13T08:36:53Z | - |
| dc.date.available | 2018-08-31 | |
| dc.date.available | 2021-05-13T08:36:53Z | - |
| dc.date.copyright | 2016-08-31 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-08-05 | |
| dc.identifier.citation | Abdel-Megeed, A. (2004). Psychrophilic degradation of long chain alkanes: VDM Verlag Dr. Muller Aktiengesellschaft & Co. KG.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/3796 | - |
| dc.description.abstract | 本研究目的主要在於探討如何利用土壤清洗的方式移除土壤中的烷類污染，並利用Rhodococcus erythropolis NTU-1能夠生物降解並包覆烷類的特性，處理溶液中的烷類，達到生物復育土壤的目的。
研究顯示，本實驗所使用的液態礦物培養基能夠清洗被烷類污染的土壤。以研究中的實驗條件為例，土壤粒徑大小為20/50 mesh、正十六烷添加量為4 ml n-C16/g soil的0.5 g土壤，經分析土壤中會含有3.2 ml n-C16/g soil，利用100 ml培養基以批次攪拌清洗10秒後，培養基中約含800 ppmv n-C16。 為了避免土壤中的水溶性物質，如有機酸或礦物質，在土壤清洗的過程中溶於清洗所使用的培養基，影響NTU-1於培養基中的生長，所以本實驗以自來水與去離子水預清洗土壤並烘乾，才將土壤用於後續實驗操作。 將清洗過土壤的培養基植入NTU-1培養72小時，初始植菌量為5 ml礦物培養基菌液(OD600nm約為1.0)。72小時之後，NTU-1可以透過生物降解與物理包覆移除95%培養基中的正十六烷。 探討影響培養基清洗效果的因素時，提高培養基的溫度有最好的清洗效果。以本研究為例，土壤粒徑大小為20/50 mesh、正十六烷添加量為4 ml n-C16/g soil的0.5 g土壤，利用100 ml培養基與污染土壤一起煮沸5分鐘，以沸騰時培養基液體的流動取代攪拌清洗。培養基能夠清洗土壤中約20%的正十六烷含量，進一步培養NTU-1達72小時後，經分析，NTU-1能移除培養基中約95%的正十六烷。 | zh_TW |
| dc.description.abstract | For bioremediation, we focused on how to apply soil washing method with MSM (mineral salt medium) to wash n-hexadecane (n-C16) on soil. Furthermore, we apply Rhodococcus erythropolis NTU-1 (NTU-1) biofloccules and biodegradation for n-hexadecane removal in MSM.
Firstly, results showed that nearly 800 ppmv n-C16 can be washed by 100 ml MSM from 0.5 g contaminated soil of which the particle size is 20/50 mesh and the added volume of pollutant is 4 ml n-C16/g soil. By analyzing with GC-FID, soil will contain 3.2 ml n-C16/g soil after added n-C16 as pollutant. Secondly, to avoid hydrosoluble compounds in soil being desolved in MSM during soil washing, which may constrain the biodegradation ability of NTU-1, we have to pre-wash soil. Being pre-washed by both tap water and DI water, soil can be used for subsequent experiments. After incubated in MSM used to wash contaminated soil for 72 hours, NTU-1 will remove approximately 95% of total n-C16 in MSM by both biodegradation and biofloccules. Last but not least, the washing effectiveness of MSM can be promoted by increasing the temperature of MSM. Results showed almost 20% of total n-C16 can be removed from 0.5 g soil whose particle size is 20/50 mesh and the added volume of pollutant is 4 ml n-C16/g soil during soil washing at 100℃. After 72 hours NTU-1 incubation, almost 95% of total n-C16 in MSM will be removed. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-13T08:36:53Z (GMT). No. of bitstreams: 1 ntu-105-R03524076-1.pdf: 3512058 bytes, checksum: fb665de1c67bce487b946145ed4c7e22 (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 中文摘要 I
Abstract II 目錄 IV 圖目錄 VII 表目錄 X 第一章 緒論 1 1.1 前言 1 1.2 研究目的與論文綱要 3 第二章 文獻回顧 4 2.1 石油碳氫化合物簡介及其對環境和人類之影響 4 2.2 處理石油碳氫化合物污染之方法 6 2.3 以微生物處理石油碳氫化合物 13 2.3.1 生物復育簡介 13 2.3.2 微生物攝取碳氫化合物之模式 18 2.3.3 微生物分解碳氫化合物之方式 26 2.4 微生物降解碳氫化合物之代謝途徑 30 2.4.1直鏈烷之氧化機制 30 2.4.2支鏈烷之氧化機制 34 2.4.3烯烴類及環烷類之氧化機制 36 2.5實驗菌株Rhodococcus erythropolis介紹 40 2.5.1 Rhodococcus菌屬介紹 40 2.5.2 Rhodococcus erythropolis特性與應用 43 2.6 微生物之細胞聚集現象 47 2.7 土壤清洗在土壤復育中的應用 49 2.8 微生物在土壤復育中的應用 53 第三章 實驗材料與方法 56 3.1 實驗材料 56 3.1.1 實驗菌株 56 3.1.2 實驗藥品與儀器 58 3.2 培養基組成與配製 59 3.2.1 液態礦物培養基 59 3.2.2 菌株保存培養基 62 3.2.3 菌株活化培養基 63 3.2.4 計數平板培養基 63 3.3 實驗方法 64 3.3.1 菌株的活化及生長曲線 64 3.3.2 礦物培養基菌液製作 65 3.3.3 正十六烷污染土壤的製備 66 3.3.4 清洗正十六烷污染的土壤 67 3.3.5 氣相層析儀之條件設定 72 3.3.6 正十六烷校正曲線 73 3.3.7 以NTU-1處理培養基中的正十六烷 74 第四章 實驗結果與討論 76 4.1 土壤實驗前的預清洗 77 4.2 清洗被正十六烷污染的土壤 81 4.2.1 篩選適合實驗的土壤的粒徑大小 81 4.2.2 土壤的污染程度 83 4.2.3 清洗被正十六烷污染土壤的方法 86 4.3 以NTU-1處理培養基中的正十六烷 90 4.4 提升液態礦物培養基對污染土壤的清洗效果 97 4.4.1 正十六烷含量計算 97 4.4.2 重複清洗污染土壤 98 4.4.3 重複使用液態礦物培養基清洗污染土壤 101 4.4.4 重複使用底部液態礦物培養基清洗污染土壤 104 4.4.5 酸鹼值對液態礦物培養基清洗效果的影響 105 4.4.6 溫度對液態礦物培養基清洗效果的影響 107 4.4.7 鹽類濃度對液態礦物培養基清洗能力的影響 112 4.4.8 以溫度提升培養基清洗效果並利用NTU-1移除正十六烷 114 4.4.9 討論 117 第五章 結論 118 第六章 參考文獻 121 | |
| dc.language.iso | zh-TW | |
| dc.subject | 生物復育 | zh_TW |
| dc.subject | 土壤清洗 | zh_TW |
| dc.subject | Rhodococcus erythropolis NTU-1 | zh_TW |
| dc.subject | 生物降解 | zh_TW |
| dc.subject | Rhodococcus erythropolis NTU-1 | en |
| dc.subject | Soil washing | en |
| dc.subject | Biodegradation | en |
| dc.subject | Bioremediation | en |
| dc.title | 以土壤清洗復育土壤並以NTU-1移除溶液中正十六烷 | zh_TW |
| dc.title | Implementing Soil Washing for Soil Remediation and Using Rhodococcus erythropolis NTU-1 for the Treatment of the Residual Solution | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 江佳穎,鄭玉佳 | |
| dc.subject.keyword | 土壤清洗,Rhodococcus erythropolis NTU-1,生物復育,生物降解, | zh_TW |
| dc.subject.keyword | Soil washing,Rhodococcus erythropolis NTU-1,Bioremediation,Biodegradation, | en |
| dc.relation.page | 134 | |
| dc.identifier.doi | 10.6342/NTU201601949 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2016-08-05 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
| 顯示於系所單位： | 化學工程學系 | |
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