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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張鴻民 | |
| dc.contributor.author | Chia-Yi Chein | en |
| dc.contributor.author | 簡佳怡 | zh_TW |
| dc.date.accessioned | 2021-06-13T07:48:56Z | - |
| dc.date.available | 2010-07-28 | |
| dc.date.copyright | 2005-07-28 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-07-26 | |
| dc.identifier.citation | 今關六也，本鄉次雄。1957。原色日本菌類圖鑑。保育社，東京。
王次男。1997。食用菇類的營養價值與藥用價值。蔗報月刊65(5):26-27。 王伯徹。2000。具開發潛力食藥用菇介紹。食品工業月刊 32(5):1-27. 沈霞芬，田九疇，薛登民，林茂勇，1996。獸醫組織學彩色圖譜。藝軒圖書出版社，臺北。 李承曄。2000。MMP蛋白酵素在白血病中之意義的研究。國立台灣大學醫事技術學研究所碩士論文。 林仁混。1972。癌是怎樣產生的。科學月刊科學月刊 3(12): 23-26。 科學月刊社。台北。 林榮耀。1972。癌∼代序。科學月刊科學月刊 3(12): 19-22。科學月 刊社。台北。 馬嘉軉。2003。黃連誘導U937細胞株行細胞凋亡之機制探討及對Balb/c公鼠之免疫調節作用。國立台灣大學食品科技研究所博士論文。 徐麗嵐。2002。以柳松菇與鴻喜菇誘導人類白血病細胞(U937)分化及對Balb/c鼠皮下移植CT26腫瘤之抑制效果。國立台灣大學食品科技研究所碩士論文。 許珮喧。2004。柳松菇及鴻喜菇萃取物對人類白血病細胞株(U937)生長抑制於G0/G1之機制。國立台灣大學食品科技研究所碩士論文。 陳健祺。2000。食用菇類在醫藥上的應用。食品工業月刊 32(5):54-69. 陳宣諭。2004。巴西洋菇對人類白血病U937細胞生長抑制機制及對Balb/c鼠尾靜脈注射CT26細胞之腫瘤轉移抑制效果。國立台灣大學食品科技研究所碩士論文。 陳宜珊。2004。絞股藍皂誘發人類肺癌細胞株(A549)的細胞週期停止在G0/G1期與計畫性死亡。中國醫藥大學醫學研究所碩士論文。 楊淑媚。2004。人參皂Rh2抑制肺腺癌細胞生長及造成凋亡之分子作用機轉研究。中國醫藥大學醫學研究所碩士論文。 歐馨婷。2001。多種蔬菜抑制人類白血病細胞U937增殖與誘導其分化之探討。國立台灣大學食品科技研究所碩士論文。 Albert B, Johnson A, Lewis J, Raff M, Roberts K, Walter P. 2002. Molecular Biology of the Cell. Garland Science, New York. Brattain MG, Strobel-Stevens J, Fine D, Webb M, Sarrif AM. 1980. Establishment of mouse colonic carcinoma cell lines with different metastatic properties. Cancer Res 40(7): 2142-2146. Birkedal-Hansen H, Moore WGI, Bodden MK, Windsor LJ, Birkedal HB, DeCarlo A, Englter JA. 1993. Matrix metalloproteinases: a review. Crit Rev Oral Biol Med 4(2):197-250. Blavier L, Henriet P, Imren S, Declerck YA. 1999. Tissue inhibitor of matrix metalloproteinases in cancer. Ann N Y Acad Sci 878:108-119. Blood CH, Zetter BR. 1990. Tumor interactions with the vasculature: angiogenesis and tumor metastasis. Biochim Biophys Acta 1032(1-2):89-118. Bode W, Fernandez-Catalan C, Grams F, Gomis-Rűth FX, Nagase H, Tschesche H, Maskos K. 1999. Insights into MMP-TIMP interactions. Ann N Y Acad Sci 878: 72-91. Cappello P, Novelli F, Forni G, Giovarelli M. 2002. Death receptor ligands in tumors. J Immunother 25(1):1-15. Chang JS, Son JK, LI G, Oh EJ Kim JY, Park SH, Bae JT, Kim HJ, Lee IS, Kim OM, Kozukue N, Han JS, Hirose M, Lee KR. 2004. Inhibition of cell cycle progression on HepG2 cells by hypsiziprenol A9, isolated from Hypsizigus marmoreus. Cancer Letters 212 (1):7-14. Chen YJ, Shieh CJ, Tsai TH, Kuo CD, Ho LT, Liu TY, Liao HF. 2005. Inhibitory effect of norcantharidin, a derivative compound from blister beetles, on tumor invasion and metastasis in CT26 colorectal adenocarcinoma cells. Anticancer Drugs 16(3):293-299. Cheung PC. 1998. Plasma and hepatic cholesterol levels and fecal neutral sterol excretion are altered in hamsters fed straw mushroom diets. J Nutr 128(9):1512-1516. Cotter TG, Lennon JG, Martin SJ. 1990. Cell death via apoptosis and its relationship it growth, development and differentiation of both tumor and normal cells. Anticancer Res 10(5A):1153-1160. Couzin J. 2003. Tracing the step of metastasis, cancer’s menacing ballet. Science 299(5609):1002-1006. Davies B, Mules DW, Haperfield LC, Naylor MS, Bobrow LG, Rubens RD, Balkwill FR. 1993. Activity of type IV collagenases in benign and malignant breast disease. Br J Cancer 67(5):1126-1131. Eischen CM, Kottke TJ, Martins LM, Basi GS, Tung JS, Earnshaw WC, Leibson PJ, Kaufmann SH. 1997. Comparison of apoptosis in wild-type and Fas-resistant cells: chemotherapy-induced apoptosis is not dependent on Fas/Fas ligand interactions. Blood 90(3):935-943. Folkman J. 1995. Angiogenesis in cancer, vascular, rheumatoid and other disease. Nat Med 1(1):27-31. Foon KA. 1989. Biological response modifiers: The new immunotherapy. Cancer Res 49(7): 1621-1639. Ford HL, Pardee AB. 1999. Cancer and the cell cycle. J Cellular Bioch 75(S32-33):166-172. Giannelli G, Falk-Marzillier J, Schiraldi O, Stetler-Stevenson WG, Quaranta V. 1997. Induction of cell migration by matrix metalloproteinase-2 cleavage of laminin-5. Science 277(5323):225- 228. Gomez DE, Alonso DF, YoshijiH, Thorgerisson UP. 1997. Tissue inhibitors of metalloproteinases: structure, regulation and biological functions. Eur J Cell Biol 74(2):111-122. Gomma K, Kraus J, Robkopf H, Roper H, Franz G. 1992. Antitumour and immunological activity of a β1-3/1-6 glucan from Glomerella cingulata. J Cancer Res Clin Oncol 118(2):136-140. Gozia O, Ciopraga J, Bentia T, Lungu M, Zamfirescu I, Tudor R, Roseanu A, Nitu F. 1993. Antifungal properties of lectin and new chitinases from potato tubers. CR Acad Sci III 316(8):788-792. Gross J, Lapiere CM. 1962. Collagenolyticactivity in amphibian tissues: a tissue culture assay. Proc Natl Acad Sci USA 48:1014-1022. Hamuro J, Takatsuki F, Suga T, Kikuchi T, Suzuki M. 1994. Synergistic antimetastatic effects of lentinan and interleukin 2 with pre- and post- operative treatments. Jpn J Cancer Res 85(12):1288-1297. Hannun YA. 1997. Apoptosis and the dilemma of cancer chemotherapy. Blood 89(6):1845-1853. Hartwell LH, Kastan MB. 1994. Cell cycle control and cancer. Science 266(5192):1821-1828. Hibino Y, Konishi Y, Koike J, Tabata T, Ohashi Y, Sugano N. 1994. Productions of interferon-g and intrite are induced in mouse splenic cells by heteroglycan-protein fraction from culture medium of Lentinus edodes mycelia. Immunopharmacology 28(1):77-85. Hikino H, Konno Y, Hayashi T. 1985. Isolation and hypoglycemic activity of ganoderans A and B, glycans of Ganoderma lucidum fruit bodies. Planta Med 4:339-340. Ho A, Dowdy SF. 2002. Regulation of G1 cell- cycle progression by oncogenes and tumor suppressor genes. Curr Opin Genet Dev 12(1):47-52. Holm B, Jensen PB, Sehested M, Hansen HH. 1994. In vivo inhibition of etoposide-mediated apoptosis, toxicity, and antitumor effect by the topoisomerase II-uncoupling anthracycline aclarubicin. Cancer Chemothe Pharmacol 34(6):503-508. Horwitz SB. 1992. Machanism of action of taxol. Trends Pharmacol Sci 15(1):134-136. Sun H, Zhao CG, Tong X, Qi YP. 2003. A lectin with mycelia differentiation and antiphytovirus activities from the edible mushroom Agrocybe aegerita. J Biochem Mol Biol 36(2):214-222. Hung K, Hayashi R, Lafond-Walker A, Lowenstein C, Pardoll D, Levitsky H. 1998. The central role of CD4(+) T cells in the antitumor immune response. J Exp Med 188(12):2357-2368. Hussein DF, Stanley Z. 2001. Matrix metalloproteinases in cancer invasion, metastasis and angiogenesis. Drug Discov Today 6(9) :478-482. Ikekawa T, Saitoh H, Feng W, Zhang H, Li L, Matsuzawa T. 1992. Antitumor activity of Hypsizigus marmoreus. I. Antitmor activity of extracts and polysaccharides. Chem Pharm Bull (Tokyo) 40(7):1954-1957. Igura K, Ohta T, Kuroda Y, Kaji K. 2001. Resveratrol and quercetin inhibit angiogenesis in vitro. Cancer Lett 171(1):11-16. Inoue T, Anderson TD, Hayes TJ, Horii I. 1996. Comparative immunotoxicity assessment of N4-Trimethoxybenzoyl-5’-deoxy-5- fluorocytidine (Ro 09-1390) and 5;-deoxy-5-fluorouridine (5’-DFUR) in BDF1 mice. J Toxicol Sci 21(1):29-39. Itoh T, Tanioka M, Yoshida H, Yoshioka T, Nishimoto H, Itohara S. 1998. Reduced angiogenesis and tumor progression gelatinase A-deficient mice. Cancer Res 58(5):1048-1051. Janeway CA, Travers P, Walport M, Shlomchik M. 2001. Garland Publishing, New York. Jong SC, Birmingham JM. 1993. Medicinal and therapeutic value of the Shiitake mushroom. Adv Appl Microbiol 39:153-184. Kazutaka T, Yutaka A, Shoji O, Takashi M, Mamoru I. 1995. Isolation of novel collagen-binding protein from the mushroom, Hypsizigus marmoreus, which inhibits the Lewis Lung carcinoma cell adhesion to Type IV collagen. J Biol Chem 270(4):1481-1484. Kim WG, Lee IK, Kim JP, Ryoo IJ, Koshino H, Yoo ID. 1997. New indole derivatives with free radical scavenging activity from Agrocybe cylinderacea. J Nat Prod 60(7):721-723. Kleiner DE, Stetlter-Stevenson WG. 1993. Structural biochemistry and activation of matrix metalloproteases. Curr Opin Cell Biol 5(5): 891-897. Kleiner DE, Stetlter-Stevenson WG. 1999. Matrix metalloproteinases and metastasis. Cancer Chem Pharm 43(Suppl):S42-S51. Kouchi Y, Maeda Y, Morinaga H, Ohuchida A. 1996. Immunotoxic effects of a new antineoplastic agent S-1 in mice-comparison with S-2, UFT and 5-FU. J Toxicol Sci 21: 691-701. Krause JR. 2000. Morphology and classification of acute myeloid leukemias. Clin Lab Med 20(1):1-16. Kuby J. 2000. Immunology. 4th ed. W. H. Freeman Co., New York. Kurata H, Thant AA, Matsuo S, Senga T, Okazaki K, Hotta N, Hamaguchi M. 2000. Constitutive activation of MAP kinase (MEK1) is critical and sufficient for the activation of MMP-2. Exp Cell Res 254(1):180-188. Lam SK, Ng TB. 2001. Hypsin, a novel thermostable ribosome- inactivating protein with antifungal and antiproliferative activities from fruiting bodies of the edible mushroom Hypsizigus marmoreus. Biochem Biophys Res Commun 285(4):1071-1075. Liao HF, Chen YY, Liu JJ, Hsu ML, Shieh HJ, Liao HJ, Shieh CJ, Sjiao MS, Chen YJ. 2003. Inhibitory effect of caffeic acid phenethyl ester on angiogenesis, tumor invasion, and metastasis. J Agric Food Chem 51(27):7907-7912. Lieu CW, Lee SS, Wang SY. 1992. The effect of Ganoderma lucidum on induction of differentiation in leukemic U937 cells. Anticancer Res 12(4):1211-1216. Liu F, Ooi VEC, Chang ST. 1997. Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci 60(10):763-771. Mataunaga K, Morita I, lijima H, Oguchi Y, Yoshimura M, Fujii T, Yoshikumi,G, Nomoto K. 1992. Effects of biological response modifiers with different modes of action used separately together on immune response in mice with syngeneic tumours. J Int Med Res 20(5):406-421. Matrisian L. 1990. Metalloproteinases and their inhibitors in matrix remodeling. Trends Genet 6(4):121-125. Matsuzawa T, Sano M, Tomita I, Saitoh H, Ikekawa T. 1997. Studies on antioxidant effect of Hypsizigus marmoreus. I. Effects of Hypsizigus marmoreus for antioxidant activities of mice plasma. Yakugaku Zasshi 117(9):623-628. Matsuzawa T, Saitoh H, Sano M, Tomita I, Ohkawa M, Ikekawa T. 1998. Studies on antioxidant effect of Hypsizigus marmoreus. II. Effects of Hypsizigus marmoreus for antioxidant activities of tumor-bearing mice. Yakugaku Zasshi 118(10):476-481. Meng Q, MalinovskiiV, Huang W, Hu Y, Chung L, Nagase H, Bode W, Maskos K, Brew K. 1999. Residure 2 of TIMP-1 is a major determinant of affinity and specificity for matrix metalloproteinases but effects of substitutions do not correlate with those of the corresponding P1’ residue of substrate. J Bio Chem 274(15): 10184-10189. Midoro K, Gotoh K, Houkan T, Yukishige K, Fujiwara K, Ootsu K. 1997. Antitumor effect of 5’-deoxy-5’-fluorouridine in combination with recombinant human interleukin 2 on murine colon carcinoma 26. Jpn J Cancer Res 88(3):306-315. Mizuno T, Wang G, Zhang J, Kawagishi H, Nishitoba T, Li J. 1995. Reishi, Ganoderma lucidum and Ganoderma tsugae: bioactive substances and medical effects. Food Reviews International 11:151-166. Moongkarndi P, Kosem N, Kaslungka S, Luanratana O, Pongpan N, Neungton N. 2004. Antiproliferation, antioxidiation and induction of apoptosis by Garcinia mangostana (mangosteen) on SKBR3 human breast cancer cell line. J Ethnopharmacol 90(1):161-166. Morigiwa A, Kitabatake K, Fujimoto Y, Ikekawa N. 1986. Angiotensin converting enzyme-inhibitory triterpenes from Ganoderma Lucidum. Chem Pharm Bull (Tokyo) 34(7):3025-3028. Murray AW, Hunt T. 1993. The Cell Cycle: An Introduction. New York: Oxford University Press. 251p. Nada O, Ivan B. 2003. Immunomodylation by water-soluble derivatives of propolis: a factor of antitumor reactivity. J Ethnopharmacol 84(2-3):265-273. Nagai Y. 1966. Tadpole collagenases. Preparation and purification. Biochemistry 5:3123-3130. Nagase H. 1997. Activation mechanisms of matrix metalloproteinases. Biol Chem 378(3-4):151-160. Nagase H, Woessner F. 1999. Matrix metalloproteinase. J Bio Chem 274(31):21491-21494. Ng TB. 1998. A review of research on the protein-bound polysaccharide (polysaccharopeptide, PSP) from the mushroom Coriolus versicolor (Basidiomycetes: Polyporaceae). Gen Pharmacol 30(1):1-4. Nicoletti I, Migliorati G, Pagliacci MC, Grignani F, Riccardi C. 1991. A rapid and simple method for measuring thymocyte apoptosis by propidium iodode staining and flow cytometry. J Immunol Method 139(2):271-279. Niles RM, McFarland M, Weimer B, Redkar A, Fu YM, Meadows GG. 2003. Resveratrol is a potent inducer of apoptosis in human melanoma cells. Cancer Lett 190(2):157-163. Nojima H. 1997. Cell cycle checkpoints, chromosome stability and the progression of cancer. Hum Cell 10(4):221-230. Norbury C, Nurse P. 1992. Animal cell cycles and their control. Anuu Rev Biochem 61: 441-470. Palozza P, Serini S, Maggiano N, Angelini M, Boninsegna A, Di Nicuolo F, Ranelletti FO, Calviello G. 2002. Introduction of cell cycle arrest and apoptosis in human colon adenocarcinoma cell lines by beta-carotene through down-regulation of cyclin A and Bcl-2 family proteins. Carcinogenesis 23(1):11-18. Park EJ, Ko G, Kim J, Sohn DH. 1997. Antifibrotic effects of a polysaccharide extracted from Ganoderma lucidum, glycyrrhizin and pentoxifylline in rats with cirrhoss induced by biliary obstruction. Biol Pharm Bull 20(4):417-420. Parsons SL, Watson SA, Brown PD. 1997. Matrix metalloproteinases. Br J Surg 84(2):160-166. Rooprai HK, McCormick D. 1997. Proteases and their inhibitors in human brain tumours: a review. Anticancer Res 17(6B):4151-4162. Saitoh H, Feng WJ, Matsuzawa T, Ikekawa T. Antitumor activity of Hypsizigus marmoreus. Preventive effect against lung metastasis of Lewis lung carcinoma. Yakugaku Zasshi 117(12):1006-1010. Sakagami H, Sugaya K, Utsumi A, Fujinaga S, Sato T, Takeda M. 1993. Stimulation by PSK of interleukin-1 production by human peripheral blood mononuclear cells. Anticancer Res 13(3):671-676. Sawicki G, Radomski W, et al. 1997. Release of gelatinase A during platelet activation mediates aggregation. Nature 386(6625):616-619. Schwartz JD, Shamamian P, Monea S, Whiting D, Marcus SG, Galloway AC, Mignatti P. 1998. Activation of tumor cell matrix metalloproteinase-2 by neutrophil proteinases requires expression of membrane-type 1 matrix metalloproteinase. Surgery 124(2):232-238. Sherr CJ. 1996. Cancer cell cycles. Science 274(5293):1672-1677. Sichel G, Corsaro C, Scalia M, DiBilio AJ, Bonomo RP. 1991. In vitro scavenger activity of some flavonoids and melanina against O2-. Free Racidal Bio Med 11(1):1-8. Smith ML, Fornace AJ. 1996. Mammalian DNA damage-inducible genes associated with growth arrest an apoptosis. Mutat Res 340(2-3):109-124. Stamenkovic I. 2000. Matrix metalloproteinases in tumor invasion and metastasis. Semin Cancer Biol 10(6):415-433. Suzuki M, Iwashiro M, Takatsuki F, Kuribayashi K, Hamuro J. 1994. Reconstitution of anti-tumor effects of Lentinan in nude mice: roles of delayed-type hypersensitivity reaction triggered by CD4-positive T cell clone in the infiltration of effector cells into tumor. Jpn J Cancer Res 85(4):409-417. Tam SC, Yip KP, Fung KP, Chang ST. 1986. Hypotensive and renal effects of an extract of the edible mushroom Pleurotus sajor-caju. Life Sci 38(13):1155-1161. Tamura R, Tanebe K, Kawanishi C, Torii K, Ono T. 1997. Effects of lentinan on abnormal ingestive behaviors induced by tumor necrosis factor. Physiol Behav 61(3):399-410. Taylor G, Rudland P. 2001. VII International congress of the metastasis reseach society. Breast Cancer Res 3(1):49-54. Telford WG, King LE, Fraker PJ. 1992. Comparative evaluation of several DNA binding dyes in the detection of apoptosis-associated chromatin degradation by flow cytometry. Cytometry 13(2):137-142. Thant AA, Sein TT, Liu E, Machida K, Kikkawa F, Koike T, Seiki, Mastuda S, Hamaguchi M. 1999. Ras pathway is required for the activation of MMP-2 secretion and for the invasion of src-transformed 3Y1. Oncogene 18(47):6555-6563. Tseng SH, Lin SM, Chen JC, Su YH, Huang HY, Chen CK, Lin PY, Chen Y. 2004. Resveratrol suppresses the angiogenesis and tumor growth of gliomas in rats. Clin Cancer Res 10(6):2190-2202. Turck J, Lovett DH et al. 1996. Matrix metalloproteinase 2 (gelatinase A) regulates glomerular mesangial cell proliferation and differentiation. J Biol Chem 271(25):15074-15083. Villunger A, Egle A, Kos M, Hartmann BL, Geley S, Kofler R, Greil R. 1997. Drug-induced apoptosis is associated with enhanced Fas (Apo-1/CD95) ligand expression but occurs independently of Fas (Apo-1/CD95) signaling in human T-acute lymphatic leukemia cells. Cancer Res. 57(16):3331-3334. Vujanovic NL. 2001. Role of TNF family ligands in antitumor activity of natural killer cells. Int Rev Immunol 20(3-4):415-437. Wang H, Ng TB, Ooi VEC. 1998. Lectins from mushrooms. Mycol Res 102(8):897-906. Wang HX, Liu WK, Ng Tg, Ooi VE, Chang ST. 1995. Immunomodulatory and antitumor activities of a polysaccharide-peptide complex from a mycelial culture of Tricholoma sp., a teal edible mushroom. Life Sci 57(3):269-281. Wang HX, Ng TB, Liu WK, Ooi VE, Chang ST. 1995. Isolation and characterization of two distinct lectins with antiproliferative activity from the cultured mycelium of the edible mushroom Tricholoma mongolicum. Int J Pept Res 46(6):508-513. Wang HX, Ng TB, Ooi VE, Liu WK, Chang ST. 1996. A polysaccharide-peptide complex from cultured mycelia of the mushroom Tricholoma mongolicum with immunoenhancing and antitumor activities. Biochem Cell Biol 74(1):95-100. Wang SY, Hsu ML, Hsu HC, Tzeng CH, Lee SS, Shiao MS, Ho CK. 1997b. The anti-tumor effect of Ganoderma lucidum is mediated by cytokines released from activated macrophages and T lymphocytes. International Journal of Cancer 70(6):699-705. Wasser SP, Weis AL. 1999. Therapeutic effects of substances occurring in higher Basidiomycetes mushroom: a modern perspective. Crit Rev Immunol 19(1):65-96. Watts AM, Shearer MH, Pass HI, Kennedy RC. 1997. Development of an experimental murine pulmonary metastasis model incorporating a viral encoded tumor specific antigen. J Virol Methods 69(1-2):93-102. Westermaurck J, Khri V. 1999. Regulation of matrix metalloproteinase expression in tumor invasion. FASEB J 13(8):781-792. Woessner JF jr. 1999. Matrix metalloproteinase inhibition. From the Jurassic to the third millennium. Ann N Y Acad Sci 878:388-403. Yamamoto Y, Shirono H, Kono K, Ohashi Y. 1997. Immunopotentiating activity of the water-soluble lignin rich fraction prepared from LEM-the extract of the solid culture medium of Lentinus edodes mycelia. Bio Biotech Biochem 61(11):1909-1912. Zakany J,Chihara G, Fachet J. 1980. Effect of lentinan on tumor growth in murine allogeneic and syngeneic hosts. Int J Cancer 25(3):371-376. Zhao C, Sun H, Tong X, Qi Y. 2003. An antitumor lectin from the edible mushroom Agrocybe aegerita. Biochem J 374(Pt2):321-327. Zucker S, Hymowitz H, Conner C, Zarrabi HM, Hurewitz AN, Montana S. 1999. Measurement of matrix metalloproteinases and tissue inhibitors of metallproteinases in blood and tissues. Clinical experimental applications. Ann N Y Acad Sci 878:213-227. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/35969 | - |
| dc.description.abstract | 鴻喜菇為低熱量、低脂肪且蛋白質含量高的食用菇，在日本培育多年，長期以來被視為高級食材且不斷推廣，目前是我國國內新興栽培的食用菇之ㄧ。文獻指出鴻喜菇具有抗腫瘤及抗真菌等之功效。本實驗中，將探討鴻喜菇40~80%硫酸銨沉澱物對小鼠大腸癌CT26腫瘤細胞株生長抑制及抗轉移之效果。細胞實驗中，利用鴻喜菇40~80%硫酸銨沉澱物與CT26細胞共同培養不同時間，以MTT法觀察其生長抑制率，並探討其對CT26細胞週期之變化。結果顯示，鴻喜菇40~80%硫酸銨沉澱物具較高的直接生長抑制效果，在濃度為600 μg/mL的生長抑制率可達67.90%以上，再經由流式細胞儀分析可知，鴻喜菇40~80%硫酸銨沉澱物可將CT26細胞週期停滯於G2/M期，並且使其走向細胞凋亡。在動物實驗中，於Balb/c鼠尾靜脈注射CT26腫瘤細胞株，建立腫瘤轉移肺臟之模式，並餵食不同劑量之鴻喜菇40~80%硫酸銨沉澱物，以探討其抗腫瘤轉移之效果。結果顯示，有餵食鴻喜菇40~80%硫酸銨沉澱物之小鼠，其腫瘤轉移至肺臟之小節結數，會隨濃度增加抑制效果愈明顯(P<0.05)。另外，於顯微鏡下觀察小鼠之肺臟及腎臟病理組織切片，於肺臟組織切片圖中，可看到腫瘤轉移後使組織間質增厚產生病變之情形；而腎臟組織切片圖顯示組織並未有病變。 | zh_TW |
| dc.description.abstract | Hypsizigus marmoreus (HM), one of popular dietary mushrooms, contains high protein, low calorie, and low fat and has long been consumed in Japan. Several researches indicate that HM has antitumor and antifungal activities. In the present study, HM was used to study its antitumor activity in vitro and in vivo. First, 40~80% ammonium sulfate precipitates of cold-salt water extracts from HM were prepared and incubated with mouse colon CT26 cells to observe the changes in growth inhibition (%) for 24, 48 and 72 hr by MTT assay, followed by the cell cycle arrest by a Flow Cytometry. Results showed that remarkable growth inhibition, higher than 68 %, was observed when CT26 cells were incubated with 600 μg/mL of 40~80 % ammonium sulfate precipitates of cold-salt water extracts from HM. In addition, cell cycle arrest of CT26 cells at G2/M phase and apoptosis were induced. In vivo, pulmonary metastasis of CT26 tumor on male Balb/c mice orally administrated for 13 days with doses (200, 400 and 600 mg/kg bw/day) of ammonium sulfate precipitates of cold-salt water extracts from HM and injected (iv) with 1×105 CT26 cells via a tail vein was established to observe the results. Metastasic lung nodules in experimental group fed with 200, 400 or 600 mg/kg bw/day decreased in a dose-dependent manner, revealing the significant (p<0.05) anti-metastatic effect of HM proteins. Histopathologic results in lung was consistent with the finding in vivo. Insignificant changes in renal and immunohistograph were observed in mice at those three dosages. | en |
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| dc.description.tableofcontents | 目錄
中文摘要…………………………………………………………………i 英文摘要…………………………………………………………………ii 第一章 前言…………………………………………………..………..1 第一節 食用菇與免疫抗癌之相關性………..……………………...1 1-1-1.1癌症……………………………..………………………….1 1-1-1.2 食品與免疫之作用……………..…………………………1 1-1-1.3免疫反應………………………..………………………….2 第二節 食用菇之簡介……………………..………………………..5 1-1-2.1 食用菇……………………………..………………………5 1-1-2.2 鴻喜菇……………………………..………………………8 壹、以鴻喜菇對CT26細胞生長抑制並探討其細胞週期之調控…...12 第三節 細胞週期之調控…………………….……………………..12 貳、以尾靜脈注射CT26之動物模式建立以探討鴻喜菇抗腫瘤轉移之能力………………………………………………………………..15 第一節 小鼠大腸癌細胞CT26之介紹……………………………15 1-2-1.1 CT26細胞株之特性……………………………………15 1-2-1.2 CT26之生瘤性…………………………………………15 第二節 癌細胞之轉移………………………………………………16 1-2-2.1 轉移………………………………………………………16 1-2-2.2 基質金屬蛋白酶…………………………………………18 1-2-2.2.1 活化…………………………………………………19 1-2-2.2.2 抑制…………………………………………………20 1-2-2.3 基質金屬蛋白酶在癌症方面之研究……………………20 1-2-2.4 組織觀察…………………………………………………22 1-2-2.4.1 組織切片……………………………………………22 1-2-2.4.2 組織切片之製作……………………………………22 第二章 鴻喜菇對小鼠大腸癌CT26細胞株生長抑制機制………...24 第一節 前言…………………………………………………………24 第二節 實驗材料……………………………………………………25 2-2.1 實驗樣品來源………………………………………………25 2-2.2 實驗細胞株…………………………………………………25 2-2.3 實驗藥品及試劑……………………………………………25 2-2.4 實驗器材……………………………………………………26 2-2.5 儀器設備……………………………………………………26 第三節 實驗方法……………………………………………………28 2-3.1 菇類鹽溶液粗萃硫酸銨沉澱物之製備……………………28 2-3.2 菇類活性物質測試…………………………………………28 2-3.3 CT26細胞株之培養………………………………………35 2-3.4 CT26生長抑制率測定……………………………………36 2-3.5 形態學上的觀察……………………………………………38 2-3.6 細胞週期生長抑制之分析測定……………………………38 2-3.7 統計分析……………………………………………………39 第四節 結果與討論………………………………………………...40 2-4.1 鴻喜菇40~80%硫酸銨沉澱物活性成分分析…………….40 2-4.2 鴻喜菇40~80%硫酸銨沉澱物電泳檢定………………….41 2-4.3 鴻喜菇40~80%硫酸銨沉澱物對CT26細胞之影響….….41 2-4.3.1 鴻喜菇40~80%硫酸銨沉澱物對CT26間接生長抑制率………………………………………………………41 2-4.3.2 鴻喜菇40~80%硫酸銨沉澱物對CT26不同時間直接生長抑制率…………………………………………........42 2-4.4 細胞週期之分析……………………………………………43 第三章 鴻喜菇經由活體之調節抑制腫瘤轉移之研究……..………56 第一節 前言………………………………………………………….56 第二節 實驗材料…………………………………………………….57 3-2.1 實驗樣品來源………………………………………………57 3-2.2 實驗動物…………………………………………………...57 3-2.3 實驗細胞株…………………………………………………57 3-2.4 實驗藥品及試劑……………………………………………57 3-2.5 實驗器材……………………………………………………58 3-2.6 儀器設備 ……………………………………………………59 第三節 實驗方法…………………………………………………....61 3-3.1 菇類鹽溶液粗萃硫酸銨沉澱物之製備………………..…61 3-3.2 CT26細胞株之冷凍、解凍及培養………………….…..61 3-3.3 動物飼養……………………………………………….…62 3-3.4 動物實驗……………………………………………….…62 3-3.5 基質金屬蛋白酶之Gelatin Zymography分析…………....67 3-3.6 統計分析……………………………………………….…..69 第四節 結果與討論…………………………………………….…..70 3-4.1 小鼠體重變化………………………………………….…..70 3-4.2 小鼠白血球數目變化………………………………….…..71 3-4.3 鴻喜菇40~80%硫酸銨沉澱物對小鼠脾臟之影響…….…72 3-4.4 鴻喜菇40~80%硫酸銨沉澱物對小鼠脾臟細胞增生之能力……………………………………………………….…..73 3-4.5 鴻喜菇40~80%硫酸銨沉澱物抑制小鼠肺臟轉移之能力…………………………………………………………...73 3-4.6 小鼠肝腎毒性測定…………………………………….…..74 3-4.7 組織病理切片………………………………………….…..75 3-4.8 小鼠脾臟細胞之條件培養液細胞激素之測定…………...76 3-4.9 鴻喜菇40~80%硫酸銨沉澱物對基質金屬蛋白酶之抑制效果…………………………………………………………...78 第四章 參考文獻…………………………………………………….94 | |
| dc.language.iso | zh-TW | |
| dc.title | 鴻喜菇蛋白質對小鼠大腸癌CT26細胞生長抑制機制
及對Balb/c鼠之該腫瘤轉移抑制效果 | zh_TW |
| dc.title | Inhibitory Effect of proteins from Hypsizigus marmoreus on CT26 Colon Adenocarcinoma Cells and Metastasis in Balb/c Mice | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 鍾景光,廖慧芬,許輔,沈立言 | |
| dc.subject.keyword | 鴻喜菇,小鼠大腸癌細胞,肺部轉移, | zh_TW |
| dc.subject.keyword | Hypsizigus marmoreus,CT26 Colon Adenocarcinoma Cells,pulmonary metastasis, | en |
| dc.relation.page | 110 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2005-07-26 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 食品科技研究所 | zh_TW |
| 顯示於系所單位： | 食品科技研究所 | |
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