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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 游若?博士 | |
| dc.contributor.author | Hsiao-Ju Pu | en |
| dc.contributor.author | 蒲孝如 | zh_TW |
| dc.date.accessioned | 2021-06-13T05:48:03Z | - |
| dc.date.available | 2006-07-28 | |
| dc.date.copyright | 2006-07-28 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-07-09 | |
| dc.identifier.citation | 台北市政府衛生局。2003。台北市政府衛生局九十二年年節食品抽驗結果第二批不合格名冊。http://www.healthcity.net.tw/upload/122.htm。
台北市政府衛生局。2004。九十三年豆製品抽驗結果名冊。http://210.69.108.165/Hhotlist.asp?DiscussID=101。 台北市政府衛生局。2005a。台北市政府衛生局九十四年年節食品抽驗結果第一波不合格名冊。http://food.doh.gov.tw/show.asp?2005117112340385。 台北市政府衛生局。2005b。台北市政府衛生局九十四年年節食品抽驗結果第二波不合格名冊。http://food.doh.gov.tw/show.asp?2005117112340385。 台灣食品良好作業規範發展協會。2005。黃豆加工食品工廠良好作業規範專則。http://www.gmp.org.tw/lawsexecution/13.doc。 行政院衛生署。2005a。一般食品類衛生標準。http://food.doh.gov.tw/chinese/ruler/hygiene_standed_3.htm#2。 行政院衛生署。2005b。食品良好衛生規範。http://food.doh.gov.tw/chinese/ruler/ruler_1_1.asp?chieng=1&lawsidx=395。 行政院衛生署。2005c。食品添加物使用範圍及其限量。http://food.doh.gov.tw/chinese/ruler/ingrdient_standard.htm。 行政院衛生署。2005d。食品衛生管理法。http://food.doh.gov.tw/chinese/ruler/ruler_1_1.asp?chieng=1&lawsidx=393。 李貽琳。1987。豆乾食品中微生物生長和安全之研究。博士學位論文。 國立台灣大學食品科技研究所。台北。 李晉嘉。2003。以反應曲面法研究生化柴油之最優酵素合成。碩士學位 論文。私立大葉大學食品工程學研究所。彰化。 周正俊。1985。豆腐、豆乾中微生物品質之探討。行政院衛生署七十五 年度科技研究發展計畫。行政院衛生署。台北。 沈明來。2000。試驗設計學。九州圖書文物有限公司，台北。 洪連發。1997。以回應曲面法探討微胞強化超過濾技術純化酪蛋 白磷胜肽之最佳條件。碩士學位論文。國立台灣大學化學工程學研究所。台北。 黃韋誠。2003。以反應曲面法探討鱈魚漿製品最適化之研究。碩士學位 論文。國立屏東科技大學食品科學研究所。屏東。 傅遠鵬。1988。食品分析方法手冊。食品工業發展研究所，新竹。 翁瑋蓮。1999。以反應曲面法探討扣碗酪之最佳生產模式。碩士 學位論文。國立臺灣大學畜產學研究所。台北。 經濟部中央標準局。2001。中國國家標準CNS食品中防腐劑之檢驗法 總號 10949 類號N6190。經濟部中央標準局。台北。 劉益忠。2002。酒釀萃微膠囊化。博士學位論文。國立台灣大學畜產學 研究所。台北。 賴茲漢、柯文慶、沈賜川。1994。乳酸發酵豆乾之製造與品質改進。食 品科學。21 (4)：256-166。 AOAC. 1984. Official Methods of Analysis of the Association of Official Analytical Chemists. 14th ed, pp.160. Bell YK, Cutter NC, Sumner SS.1997. Reduction of foodborne microorganisms on beef carcass tissue using acetic acid, sodium bicarbonate, and hydrogen peroxide spray washes. Food Microbiol 14: 439–448 Blocher JC, Busta FF. 1985. Multiple modes of inhibition of spore germination and outgrowth by reduced pH and sorbate. J Appl Bacteriol 59: 467–478. Booth IR, Kroll RG. 1989. The preservation of foods by low pH. In: Gould, G.W. (Ed.). Mechanisms of Action of Food Preservation Procedures. Elsevier. London. pp. 119–160. Box GEP, Behnken DW. 1960. Some new three level designs for the study of quantitative variables. Technometrics 2: 455–475. Box GEP, Hunter WG, Hunter JS. 1978. Statistics for Experimenters. John Wiley & Sons, Inc. New York. pp. 510–539 Box GEP, Wilson KB. 1951. On the experimental attainment optimum conditions. J Roy Statist Soc B13: 1–45 Brula S, Cooteb P. 1999. Review: Preservative agents in foods. Mode of action and microbial resistance mechanisms. Int J Food Microbiol 50: 1–17 Burt S. 2004. Review Essential oils: their antibacterial properties and potential applications in foods- a review. International J Food Microbiol 94: 223– 253 Campbell. 2000. Acids and bases. In: Biochemistry 3ed edn. Harcourt Brace & Company. Florida. pp.55–64 Cappuccino JG, Sherman N. 1999. The bacterial growth curve. In: Microbiology A Laboratory manual. Addison Wesley Longman, Inc. New York. pp.125–132. Chang YN, Huang JC, Lee CC, Shih IL, Tzeng YM. 2002. Use of response surface methodology to optimize culture medium for production of lovastatin by Monascus rubber. Enzym Microb Tech 30: 889–894 Ellerbroek L. 1997. Airborne microflora in poultry slaughtering establishments. Food Microbiol 14: 527–531 FDA. 2004. Protecting the Food Supply. http://www.cfsan.fda.gov/~dms/fsbtac21.html Ferrand C, Marc F, Fritsch P, Cassand P, Blanquat SG. 2000. Genotoxicity Study of Reaction Products of Sorbic Acid. J Agric Food Chem 48: 8: 3605–3610 Fouad KE, Hegeman GD. 1993. Microbial spoilage of tofu. J Food Protect 56(2): 157–164 Geornaras I, Jesus AE, Zyl E, Holy A. 1996. Bacterial population associated with poultry processing in a South African abattoir. Food Microbiol 13: 457–465 Holley RA, Patel D. 2005. Review: Improvement in shelf-life and safety of perishable foods by plant essential oils and smoke antimicrobials. Food Microbiol 22: 273–292 Holyoak CD, Stratford M, McMullin Z, Cole MB, Crimmins K, Brown AJP, Coote P. 1996. Activity of the enplasma- membrane H1-ATPase and optimal glycolytic flux are required for rapid adaptation and growth in the presence of the weak acid preservative sorbic acid. Appl Environ Microbiol 62: 3158–3164. Inatsu Y, Bari L, Kawasaki S, Isshiki K, Kawamoto S. 2005. Efficacy of acidified sodium chlorite treatments in reducing Escherichia coli O157:H7 on Chinese Cabbage. J Food Protect 68 (2): 251–252 Jago GR, Morrison M. 1962. Anti-streptococcal activity of lactoperoxidase. Proc Soc Exp Biol Med 111: 585–588. Krebs HA, Wiggins D, Sole S, Bedoya F.1983. Studies on the mechanism of the antifungal action of benzoate. Biochem J 214: 657–663. Lakshmanan R, Jeya SR, Jeyasekaran G. 2002. Survival of amine-forming bacteria during the ice storage of fish and shrimp. Food Microbiol 19: 617–625 Lambert RJ, Stratford M. 1999. Weak-acid preservatives: modeling microbial inhibition and response. J Appl Microbiol 86: 157-164 Leistner L. 2000. Basic aspects of food preservation by hurdle technology. Int J Food Microbiol 55: 181–186 Leistner L, Gorris GM. 1995. Food preservation by hurdle technology. Trends Food Sci Tech 6: 41–46 Li C, Bai JH, Cai ZL, Fan OY. 2002. Optimization of a cultural medium for bacteriocin production by Lactococcus lactis using response surface methodology. J Biotechnol 93: 27–34 Mahmoud BSM, Yamazaki K, Miyashita K, Shin IS. 2004. Bacterial microflora of carp (Cyprinus carpio) and its shelf-life extension by essential oil compounds. Food Microbiol 21: 657–666 Myers RH, Montgomery DC. 2002. Response surface methodology: process and product optimization using designed experiments. John Wiley & Sons, Inc. New York. Palmer SA, Stewart J, Fyfe L. 2001. The potential application of plant essential oils as natural food preservatives in soft cheese. Food Microbiol 18: 463– 470 Pelczar MJ, Reid Jr RD, Chan ECS. 1986. Microbiology. McGraw-Hill, New York. Piper P, Mahe Y, Thompson S, Pandjaitan R, Holyoak C, Egner R, Muhlbauer M, Coote P, Kuchler K. 1998. The Pdr12 ATP-binding cassette ABC is required for the development of weak acid resistance in Saccharomyces cerevisiae. EMBO J 17: 4257–4265 Reiter BA, Picking JD, Oram, Pope G.S. 1963. Peroxidase /thiocyanate inhibition of streptococci in raw milk. J Gen Microbiol 33:12–15. Roller S. 1999. Physiology of food spoilage organisms. Int J Food Microbiol 50: 151–153 Sofos JN, Busta FF. 1981. Antimicrobial activity of sorbate. J Food Protect 44: 614–622. Su SC, Liu CH, Chen HC, Chang PC, Chou SS. 1999. Studies on the determination of hydrogen peroxide and its dissipation in food. J Food Drug Anal 7 (2): 131–142. Thomson D. 1982. Response surface experimentation. J Food Sci 6: 155–188 Toropov V. 2003. Chapter 3 Response surface methodology. http://www.brad.ac.uk/staff/vtoropov/ Tyrer H, Ainsworth P, Ibanoglu S, 2004. Modeling the growth of Pseudomonas fluorescens and Candida sake in ready-to-eat meals. J Food Eeg 65: 137–143 Venkitanarayanan K S, Zhao T, Doyle M P. 1999. Inactivation of Escherichia coli O157: H7 by combinations of GRAS chemicals and temperature. Food microbiol 16: 75– 82 Venturini ME, Oria R, Blanco D. 2002. Microflora of two varieties of sweet cherries: Burlat and Sweetheart. Food microbiol 19: 15–21 Virginia M. Weaver, Timothy Buckley, and John D. Groopman. 2000. Lack of specificity of trans,trans-muconic acid as a benzene biomarker after Ingestion of sorbic acid-preserved foods. Canc Epidemiol Biomarkers Prev 9: 749–755 Wang DS, Torng CC, Lin IP, Cheng BW, Liu HR, Chou CY. 2006. Optimization of nattokinase production conduction using response surface methodology. J Food Proc Engin 29: 1: 22–35 Wesierska E, Saleh Y, Trziszka T, Kopec W, Siewinski M, Korzekwa K. 2005. Antimicrobial activity of chicken egg white cystatin. World J Microbiol Biotechnol 21: 59–64 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/33879 | - |
| dc.description.abstract | 本研究進行桃園大溪地區豆腐乾製程與產品之衛生概況結果為生豆、豆乳至豆腐乾，半成品至成品的過程中，水分含量70 %至90 %不等，pH值介於6至7左右，為一般微生物適合生長的環境。該地區豆腐乾防腐劑添加過量的情況已豆腐乾類產品最為嚴重，11件豆腐乾樣品中有6件苯甲酸使用過量的情形。桃園縣大溪地區市售四種豆腐乾產品於25℃儲存12小時後，除了過氧化氫殘留量超過0.1 %之產品菌數沒有明顯增加外，菌數均達107 CFU/g~108 CFU/g，且表面有粘性物質產生及不良氣味，於5℃之儲存下市售產品儲存期間菌數之下降不明顯。實驗自四種豆腐乾產品中所分離出之腐敗微生物為Acinetobacter lwoffii，此菌為不具發酵特性之格蘭氏陰性桿菌。
本實驗目的為應用符合GRAS之物質於豆腐乾製程以改目前善大溪地區豆腐乾之儲存情況與衛生品質。依據反應曲面法之結果找出培養溫度25℃下，苯甲酸濃度400~500 ppm、磷酸濃度300~400 ppm時，Serratia liquefaciens有較低之平均生長速度0.002至0.004 (OD600/hr)。中藥水溶液對豆腐乾腐敗菌之抑制實驗結果丁香與肉桂對A. lwoffii最小抑制濃度為3 % (w/v)，丁香對P. fluorescens最小抑制濃度為16 %，大茴香對S. liquefaciens最小抑制濃度為6 %。 豆腐乾經由以下列滷汁配方：苯甲酸420 ppm、檸檬酸鈉300 ppm或磷酸300 ppm、10 %中藥(肉桂:大茴香:丁香=3 :4 :3)以100℃滷煮15分鐘處理後，豆腐乾pH値從6.4降至5.5或5.2，並且於25℃儲存二天，表面未有粘性物質產生且無不良氣味，相較於大溪地區市售豆腐乾之儲存情況儲存期限從12小時延長至48小時，4℃下豆腐乾之總生菌數 隨著儲存時間之延長而下降，顯示本實驗配方可延緩豆腐乾在儲存期間之腐敗速度。 | zh_TW |
| dc.description.abstract | The dried soybean curds from Taoyuan County contains about, 70-90 % water, and water activity is about 0.8.The pH is around 6.0 - 7.0 which provides a good growth condition for spoilage organisms. Generally, after 12 hours of storage under room temperature, dried soybean curd will spoil and form slime on the surface. The spoilage organism isolated from dried soybean curd of Taoyuan County in this study was found to be Acinetobacter sp.
Objective of this study is to improve the microbial quality of dried soybean curd in Taoyuan- Daxi area by combine several GRAS materials. According to the results of RSM, we decide the use of benzoic acid (420 ppm) and phosphoric acid (300 ppm) in treating dried soybean curd when the temperature of environment is 25℃ and the pH of soybean curd is 5 to minimize the growth of Serratia liquefaciens. The growth of indicator organisms, Serratia liquefaciens, can be inhibited by anise, cinnamon and clove and the minimum inhibitory concentration for anise, cinnamon, and clove are 6 %, 12% and 20 %, respectively. Pseudomonas fluorescens can be inhibited by clove, with a minimum inhibitory concentration of 16 %. While Acinetobacter lwoffii can be inhibited by cinnamon and clove, both with a minimum inhibitory concentration of 3 %. The combinations of benzoic acid 420 ppm, phosphoric acid or citric acid 300 ppm, cinnamon 3 %, anise 4 %, and clove 3 % in the bittern could extend the shelf life of dried soybean curd from 12 hours to 48 hours under 25℃. The total count of dried soybean curd decrease with the storage time under 5℃. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T05:48:03Z (GMT). No. of bitstreams: 1 ntu-95-R93641028-1.pdf: 2015693 bytes, checksum: a8abad755b302594923779760dbb64f5 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | 壹、前言................................................1
貳、文獻整理............................................2 一、豆製品之簡介........................................2 (一) 豆乾、豆腐.......................................2 (二) 市售豆乾食品之主要衛生安全問題.....................2 (三) 易腐敗食品之定義...................................2 (四) 衛生署食品衛生之相關規定...........................3 (五) 造成豆製品污染常見之細菌...........................3 1. Lactic acid bacteria.................................3 2. Pseudomonas..........................................3 3. Enteric bacteria.....................................4 4. Streptococci.........................................4 5. 其他.................................................4 二、常使用於食品中之防腐物質............................4 (一) 弱酸...............................................4 (二) 過氧化氫...........................................5 (三) 天然防腐物質.......................................6 (四) 豆乾常用之防腐劑...................................8 三、柵欄技術............................................9 (一) 柵欄技術之簡介 ....................................9 (二) 從微生物生理學來看柵欄技術之優點..................13 1. 柵欄技術與微生物的內平衡............................13 2. 柵欄技術與微生物的能量耗盡..........................13 3. 柵欄技術與微生物的緊迫反應..........................14 (三) 食品中的防腐柵欄因子..............................14 1. 物理性柵欄..........................................15 2. 物理化學柵欄........................................15 3. 微生物柵欄..........................................15 4. 其他柵欄............................................15 (四) 柵欄技術與食品的品質..............................15 四、反應曲面法.........................................18 (一) 反應曲面法簡介....................................18 (二) 反應曲面法之原理基本概念..........................19 (三) 反應曲面法之數學模式介紹..........................20 (四) 最佳化配方搜尋過程................................23 (五) 反應曲面法實驗設計步驟............................24 1.確定因數.............................................24 2.選擇適當層階.........................................24 3.確定決定品質之測量方法後進行實驗之設計...............24 (六) 常用之反應曲面法實驗設計..........................24 1.二階層因數設計.......................................24 2.中心混層設計.........................................25 (七) 反應曲面模式適切性之統計檢驗......................27 (八) 反應曲面法的優點..................................27 參、實驗架構與研究目的.................................30 肆、材料與方法.........................................33 一、試驗材料...........................................33 (一) 原料..............................................33 (二) 試驗菌株..........................................33 (三) 培養基............................................33 (四)藥品...............................................34 (五) 儀器設備與消耗器材................................34 二、試驗方法...........................................36 (一)豆乾廠房空中落菌檢測............................36 (二)豆乾製程中各項半成品性狀調查.......................36 (三)市售豆乾防腐劑添加量檢測...........................36 (四)市售豆乾過氧化氫殘留量檢測.........................38 (五)豆乾微生物菌相測定.................................38 (六)市售豆乾酸鹼度、水活性及水分含量分析...............39 (七)市售豆乾腐敗指標微生物分離與鑑定...................39 (八)儲存試驗...........................................39 (九)統計分析...........................................40 (十)菌株之保存與活化...................................40 (十一)微生物生長測定...................................40 (十二)微生物平均生長速度測定...........................40 (十三)反應曲面法之試驗設計.............................41 (十四)反應曲面法最適配方搜尋...........................42 (十五)中藥水溶液製備...................................42 (十六)中藥水溶液抑制實驗...............................42 (十七)滷汁製備.........................................43 (十八)儲存試驗.........................................43 (十九)反應曲面法之統計分析.............................43 伍、結果與討論.........................................45 一、大溪地區豆乾生產現況調查...........................45 (一)豆乾製程與廠房環境衛生狀況.........................45 (二)市售豆乾防腐劑添加量與過氧化氫殘留量檢測...........47 (三)市售豆乾儲存試驗...................................47 (四) 市售豆乾腐敗指標微生物分離與鑑定..................48 二、以反應曲面法配合中藥水溶液抑制實驗探討豆乾滷汁中柵欄因 子抑制指標微生物之最佳配方.............................49 (一)選擇指標微生物.....................................49 (二)反應曲面數學模式之建立.............................49 (三)培養液酸鹼度對Serratia liquefaciens平均生長速度之影響.....................................................52 (四)培養液中苯甲酸及磷酸濃度對Serratia liquefaciens平均生長速度之影響.............................................52 (五)抑制豆乾腐敗微生物Serratia liquefaciens生長之最適條件探 討....................................................53 (六)中藥水溶液對豆乾腐敗菌之抑制作用...................53 (七)中藥水溶液對豆乾腐敗菌之最小抑制濃度...............54 (八)儲存試驗...........................................55 1.滷汁與豆乾酸鹼度於滷煮前後之變化............55 2.豆乾於室溫下儲存期間之生菌數變化............55 3.豆乾於冷藏儲存期間之生菌數變化..............56 陸、結論...............................................80 柒、參考文獻...........................................82 | |
| dc.language.iso | zh-TW | |
| dc.subject | 豆乾 | zh_TW |
| dc.subject | 柵欄技術 | zh_TW |
| dc.subject | 衛生 | zh_TW |
| dc.subject | microbial quality | en |
| dc.subject | dried soybean curd | en |
| dc.subject | hurdle technology | en |
| dc.title | 柵欄技術應用於桃園大溪地區豆腐乾衛生品質之改善 | zh_TW |
| dc.title | Improvement of Microbial Quality of Dried Soybean Curd of Taoyuan - Daxi Area by Hurdle Technology | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 蔡國珍博士,潘崇良博士,丘志威博士,周正俊博士 | |
| dc.subject.keyword | 豆乾,衛生,柵欄技術, | zh_TW |
| dc.subject.keyword | dried soybean curd,microbial quality,hurdle technology, | en |
| dc.relation.page | 88 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-07-11 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 食品科技研究所 | zh_TW |
| 顯示於系所單位： | 食品科技研究所 | |
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