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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 游若? | |
dc.contributor.author | I-Ting Hsu | en |
dc.contributor.author | 許怡亭 | zh_TW |
dc.date.accessioned | 2021-06-13T04:35:39Z | - |
dc.date.available | 2006-07-24 | |
dc.date.copyright | 2006-07-24 | |
dc.date.issued | 2006 | |
dc.date.submitted | 2006-07-19 | |
dc.identifier.citation | 于守洋, 崔洪斌。2003。新世紀保健食品全集。台北。九州圖書文物有限公司。
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/33344 | - |
dc.description.abstract | 本實驗以常見的益生性乳酸桿菌及雙叉桿菌為研究對象,檢測其在乳糖基質的生長條件下生產胞外多醣的能力,並選取各菌屬中產量最高者,探討影響其生成的培養條件與特性,最後分析胞外多醣生合成的關鍵性酵素活性與代謝的可能途徑。結果顯示,在以乳糖為基質之化學組成培養基(chemical defined medium, CDM),Lactobacillus salivarius BCRC14759與Bifidobacteria bifidium BCRC 14615有最高的胞外多醣產量( 29.2 mg/L, 8.6 mg/L );在不同培養條件下,L. salivarius BCRC14759 於含5 g/L乳糖濃度培養基,以40℃的培養溫度下,有最高的胞外多醣產量(50 mg/L),B. bifidium BCRC 14615則在含40 g/L乳糖培養基,35℃培養溫度下,胞外多醣產量最高(19 mg/L)。L. salivarius BCRC14759與B. bifidium BCRC 14615之胞外多醣產量與菌體生長有相關性,對培養基中的葡萄糖利用性較半乳糖高;兩菌株的胞外多醣生合成酵素α- phosphoglucomutase 、UDP-glucose pyrophosphorylase及 UDP-galactose-4-epimerase 的比活性皆比其他生成酵素高,有利於glucose-1-P、UDP-glucose與UDP-galactose之生成,推測其合成胞外多醣途徑係經由葡萄糖轉變成UDP-glucose至UDP-galactose,最後生成胞外多醣。 | zh_TW |
dc.description.abstract | This study was conducted to investigate the production of exopolysaccharide (EPS) from several probiotic Lactobacillus and Bifidobacterium species in lactose media. The highest EPS production of Lactobacillus and Bifidobacterium were then assayed for the effects of incubation conditions on their EPS production. Besides, the key enzymes involving in the biosynthesis of EPS in Lactobacillus and Bifidobacterium were assayed. The results show that Lactobacillus salivarius BCRC14759 and Bifidobacteria bifidum BCRC 14615 are the highest EPS producers while they were grown in chemical defined media (CDM) containing lactose. The production of EPS by L. salivarius BCRC14759 and B. bifidum BCRC 14615 were 29.2 mg/L and 8.6 mg/L, irrespectively. While cultivation at 40℃and in CDM that contain 5 g/L lactose, the production of EPS by L. salivarius BCRC14759 was further increased to 50 mg/L. When B. bifidum BCRC 14615 was cultivated in CDM that containing 40 g/L lactose and incubation at 35℃, the production of EPS was increased to 19 mg/L. The glucose utilization was found higher than galactose in L. salivarius BCRC14759 and B. bifidum BCRC 14615. The production of EPS was related to the growth of these bacteria. The α- phosphoglucomutase, UDP-glucose pyrophosphorylase and UDP-galactose-4-epimerase were involved in the biosynthesis of EPS. Their specific activities were higher than other biosynthetic enzymes and utilized in the synthesis of glucose-1-P, UDP-glucose and UDP-galactose. Therefore, the pathway for EPS biosynthesis in L. salivarius BCRC14759 and B. bifidum BCRC 14615 was probably via glucose to UDP-glucose, UDP-galactose, and EPS. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T04:35:39Z (GMT). No. of bitstreams: 1 ntu-95-R93641021-1.pdf: 1593972 bytes, checksum: aba00e24f1542b34271d9c9e3d044313 (MD5) Previous issue date: 2006 | en |
dc.description.tableofcontents | 壹、前言.. .. .. .. .. .. .. .. .. .. .. .. ..1
貳、文獻整理.. .. .. .. .. .. .. .. .. .. .. ..3 一、乳酸菌.. .. .. .. .. .. .. .. .. .. .. ..3 二、乳酸菌的種類.. .. .. .. .. .. .. .. .. .. 4 三、雙叉桿菌.. .. .. .. .. .. .. .. .. .. .. .6 四、益生菌.. .. .. .. .. .... .. .. .. .. .. 8 (一)益生菌之定義.. .. .. .. .. .. .. .. .. 8 (二)益生菌之健康功效.. .. .. .. .. .. .. .. 8 1. 維持腸道菌相平衡.. .. .. .. .. .. .. 10 2. 預防腹瀉及舒緩其症狀.. .. .. .. .. .. 10 3. 改善乳糖不耐症.. .. .. .. .. .. .. 11 4. 降低結腸癌發生率.. .. .. .. .. .. .. 11 5. 改善便秘.. .. .. .. .. .. .. .. .. 12 6. 降低膽固醇.. .. .. .. .. .. .. .. 12 7. 強化免疫系統.. .. .. .. .. .. .. .. 12 8. 改善過敏現象.. .. .. .. .. .. .. .. 13 (三)益生菌之特性與應用.. .. .. .. .. .. .. 16 五、乳酸菌胞外多醣.. .. .. .. .. .. .. .. .. 19 (一) 乳酸菌胞外多醣之分類、組成及結構.. .. .. ..19 (二) 乳酸菌生產胞外多醣之特性.. .. .. .. .. .. 20 (三) 影響乳酸菌胞外多醣生成之因子.. .. .. .. .. 23 (四) 乳酸菌胞外多醣之應用.. .. .. .. .. .. .. 24 六、乳酸菌胞外多醣之生合成代謝途徑.. .. .. .. .. .27 參、材料與方法.. .. .. .. .. .. .. .. .. .. .. 32 一、實驗材料.. .. .. .. .. .. .. .. .. .. .. 32 (一)試驗菌株.. .. .. .. .. .. .. .. .. .. 32 (二)培養基.. .. .. .. .. .. .. .. .. .. 32 (三)藥品.. .. .. .. .. .. .. .. .. .. .. 34 (四)儀器設備與器材.. .. .. .. .. .. .. .. 34 二、實驗方法.. .. .. .. .. .. .. .. .. .. .. 36 (一)菌株之保存與活化.. .. .. .. .. .. .. .. 36 (二)胞外多醣的產量測定.. .. .. .. .. .. .. 37 (三)胞外多醣之生成條件試驗.. .. .. .. .. .. 37 (四) 殘餘乳糖量、釋出的葡萄糖與半乳糖量之測定.. .. 38 (五) 參與胞外多醣生成之酵素活性分析.. .. .. .. 39 (六) 統計分析方法.. .. .. .. .. .. .. .. .. 42 肆、結果與討論.. .. .. .. .. .. .. .. .. .. .. 43 一、十三株乳酸桿菌與六株雙叉桿菌在化學定量組成培養基中 胞外多醣之產量.. .. .. .. .. .. .. .. .. 43 二、不同培養條件對L.salivarius BCRC 14759與B.bifidium BCRC 14615胞外多醣產量之影響.. .. .. .. .. 43 (一) 不同乳糖濃度之化學定量組成培養基對L.salivarius BCRC 14759胞外多醣產量之影響.. .. .. .. .. 46 (二) 不同乳糖濃度之化學定量組成培養基對B.bifidium BCRC 14615胞外多醣產量之影響.. .. .. .. .. 46 (三) 不同培養溫度對L. salivarius BCRC 14759胞外多醣 產量之影響.. .. .. .. .. .. .. .. .. 49 (四) 不同培養溫度對B. bifidium BCRC 14615胞外多醣產 量之影響.. .. .. .. .. .. .. .. .. .. 49 三、L. salivarius BCRC 14759與B. bifidium BCRC 14615 於最適培養條件下,其生長情形與胞外多醣產量、糖類 利用性之變化.. .. .. .. .. .. .. .. .. .. 52 (一) L. salivarius BCRC 14759之生長曲線與胞外多醣產 量之變化.. .. .. .. .. .. .. .. .. .. 52 (二) B. bifidium BCRC 14615之生長曲線與胞外多醣產量 之變化.. .. .. .. .. .. .. .. .. .. 53 (三) L. salivarius BCRC 14759之生長與糖類利用性之變 化.. .. .. .. .. .. .. .. .. .. .. ..53 (四) B. bifidium BCRC 14615之生長與糖類利用性之變 化.. .. .. .. .. .. .. .. .. .. .. 56 四、L. salivarius BCRC 14759與B. bifidium BCRC 14615 於最適培養條件下,生成胞外多醣之關鍵性酵素活性的 變化.. .. .. .. .. .. .. .. .. .. .. .56 (一) L. salivarius BCRC 14759生成胞外多醣之關鍵性酵 素活性變化.. .. .. .. .. .. .. .. .. 56 (二) B. bifidium BCRC 14615生成胞外多醣之關鍵性酵素 活性變化.. .. .. .. .. .. .. .. .. .. 59 伍、結論.. .. .. .. .. .. .. .. .. .. .. .. 62 參考文獻.. .. .. .. .. .. .. .. .. .. .. .. 64 | |
dc.language.iso | zh-TW | |
dc.title | 乳酸桿菌及雙叉桿菌之胞外多醣生成條件與關鍵性酵素活性研究 | zh_TW |
dc.title | Analysis of exopolysaccharide production and key enzyme activities of several lactobacilli and bifidobacteria | en |
dc.type | Thesis | |
dc.date.schoolyear | 94-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 周正俊,丘志威,蔡國珍,潘崇良 | |
dc.subject.keyword | 乳酸桿菌,雙叉桿菌,胞外多醣,酵素, | zh_TW |
dc.subject.keyword | Lactobacillus,Bifidobacteria,exopolysaccharide,enzyme, | en |
dc.relation.page | 73 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2006-07-20 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 食品科技研究所 | zh_TW |
顯示於系所單位: | 食品科技研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-95-1.pdf 目前未授權公開取用 | 1.56 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。