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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 葉安義 | |
| dc.contributor.author | Ying-Yin Liu | en |
| dc.contributor.author | 劉盈吟 | zh_TW |
| dc.date.accessioned | 2021-06-13T02:11:23Z | - |
| dc.date.available | 2007-07-03 | |
| dc.date.copyright | 2007-07-03 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-06-22 | |
| dc.identifier.citation | 食品資訊網。基因改造食品安全性評估方法。http://food.doh.gov.tw/chinese/gmo/gmo8.htm, Accessed June 8, 2007
陳時欣。2006。蔗糖酯對奈米/次微米纖維素懸浮液穩定性之研究。國立臺灣大學 食品科技研究所，碩士論文。台北市。 趙明煜。2004。奈米纖維製備方法之研究。國立臺灣大學 食品科技研究所，碩士論文。台北市。 葉安義，2004。奈米科技於食品之應用。科學發展，384:44-49. ACNFP (The Advisory Committee on Novel Foods and Processes). Novel food assessments. http://www.acnfp.gov.uk/assess/#id_299481, Accessed June 8, 2007 Brusick D. 1983. Mutagenicity and carcinogenicity correlations between bacteria and rodents. In: William GM, Dunkel VC, Ray VA, editors. Annals of the New York Academy of Sciences: cellular systems for toxicity testing, Vol. 407. New York: New York Academy of Sciences. p. 164–76. Brusick D. 1984. Genetic toxicology: applications and testing strategies. In: Douglas JF, editor.Carcinogenesis and mutagenesis testing. Clifton, N.J.: TheHumana Press. p 7–16. Choi C. 2002. For personalized beverage just add microscopic liquid. United Press International. http://www.smalltimes.com, Accessed June 8, 2007 Council Regulation 258/97/EC. 1997. Official Journal L 043. 14/02. p. 1-7. Edenharder R., Leopold CH., Kries M. 1995. Modifying actions of solvent extracts from fruit and vegetable residues on 2-amino-3-methylimidazo [4,5-f] quinoline (IQ) and 2-amino-3,4-dimethylimidazo [4,5-f] quinoxaline (MelQx) induced mutagenesis in Salmonella typhimurium TA 98. Mutat. Res. 341:303-318. Gardner E. 2003. Brainy food: academia, industry sink their teeth into edible nano. Small Time Correspondent. http://www.smalltimes.com, Accessed June 8, 2007) Huang LC., Clarkin KC., Wahl GM. 1996. Sensitivity and selectivity of the DNA damage sensor responsible for activating p53-dependent G1 arrest. Proc. Natl. Acad. Sci. USA 93: 4827-4832. Maron DM., Ames BN. 1983. Revised methods for the Salmonella mutagenicity test. Mutat. Res. 113:173-215. Oberdörster G., Maynard A., Donaldson K., Castranova V., Fitzpatrick J., Ausman K., Carter J., Karn B., Kreyling W., Lai D., Olin S., Monteiro-R. N., Warheit D., Yang H. 2005. Principles for characterizing the potential human health effects from exposure to nanomaterials: elements of a screening strategy. Particle and Fibre Toxicology. 2:8. OECD Paris. 1993. Safety Evaluation of Foods Produced by Modern Biotechnology - concepts and principles. Ong T., Wong WZ., Stewart JD., Brockman HE. 1986. Chlorophyllin: a potent antimutagen against environmental and dietary complex mixtures. Mutat. Res. 173, 111–115. Peterson MD., Mooseker MS. 1992. Characterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2. J. Cell Sci. 102: 581-600 Plumb JA., Milroy R., Kaye SB. 1987. Optimization of a chemosensitivity assay based on reduction of the tetrazolium dye, MTT. Anticancer res. 7 (5): 902-902 Part A. Quaroni A., Wands J., Robert L., Trelstad RL., Isselbacher KJ. 1978. Fibronectin synthesis by epithelial crypt cells of rat small intestine. Proc. Natl. Acad. Sci. USA 75: 5548-5552 Quaroni A., Wands J., Trelstad RL., Isselbacher KJ. 1979. Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria. J. Cell Biol. 80: 248-265. Report of a joint FAO/WHO consultation, WHO Geneva. 1991. Strategies for assessing the Safety of Foods Produced by Biotechnology. Sanguansri P. and Augustin MA. 2006. Nanoscale materials development - a food industry perspective. Trends food sci. technol. 17:547-556 Sekizawa J., Shibamoto T. 1986. Salmonella/microsome mutagenicity tests of heatprocessed milk samples. Food Chem Toxicol 24(9):987–8. Shibata, T. 2002. Method for producing green tea in microfine powder. United States Patent US6416803B1. Soto KF., Carrasco A., Powell TG., Garza KM., Murr LE. 2005. Comparative in vitro cytotoxicity assessment of some manufactured nanoparticulate materials characterized by transmission electron microscopy. Journal of Nanoparticle Research. 7: 145–169 Wolfe J. 2005. Safer and guilt-Free nano foods. Forbes/Wolfe Nanotech Report. Joseph T.and Morrison M. 2006. Nanotechnology in Agriculture and Food. A Nanoforum report, available for download from www.nanoforum.org. Zhang YHP., Lynd LR. 2004. Kinetics and relative importance of phosphorolytic and hydrolytic cleavage of cellodextrins and cellobiose in cell extracts of Clostridium thermocellum. Appl. Environ. Microbiol. 70(3):1563-9. http://www.forbes.com/investmentnewsletters/2005/08/09/nanotechnology-kraft-hershey-cz_jw_0810soapbox_inl.html?partner=rss, Accessed Nov. 10, 2005. http://www.fda.gov/bbs/topics/NEWS/2006/NEW01426.html Accessed June 8, 2007 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/30655 | - |
| dc.description.abstract | 本研究以纖維素為材料，使用奈米級介質研磨機，以由上而下（top-down）方式，將纖維素巨分子以機械力撞擊降解成奈米/次微米之纖維素顆粒懸浮液，並探討其性質與安全性。以肉眼觀察懸浮液、凍乾粉末以及烘乾粉末之差異，再使用光學顯微鏡觀察懸浮液中纖維素次顆粒的聚集情形、掃瞄式電子顯微鏡觀察凍乾粉末以及烘乾粉末之表面組成。形態觀察結果顯示奈米/次微米化纖維素和纖維素原料大不相同，並由雷射粒徑分析儀的粒徑分佈結果，確認奈米/次微米化纖維素懸浮液之顆粒組成大小為奈米/次微米等級。保水力方面，研磨過後之纖維素保水力比原料高約8.5倍，與比表面積增加量一致。安全性評估方面，以沙門氏菌逆突變試驗（Ames test）TA98、TA100菌系評估奈米/次微米纖維素之致突變性，結果皆顯示試驗組所有濃度之回復菌落數均無超過對照組兩倍以上，且亦無濃度效應，判定其不具有致突變性。細胞毒性試驗則顯示奈米/次微米纖維素對於目標接觸細胞株：鼠腸道細胞株IEC-6、人類皮膚纖維母細胞株WS1以及人類腸道上皮細胞株Caco-2均無顯著的細胞毒殺效應。本研究更進一步探討奈米/次微米纖維素的抗致突變性，以沙門氏菌逆突變試驗檢測TA98、TA100菌系抗4-NQO之能力，結果顯示奈米/次微米化纖維素於TA98菌系無顯著的抗致突變效果；TA100菌系則具有些微抗致突變效果。 | zh_TW |
| dc.description.abstract | In this research, nano/submicron cellulose suspension is produced by “top-down” approach of media milling. In this approach, raw cellulose is degraded by mechanical force, and its characteristics and safety is investigated as well. In addition, the morphological differences between raw and nano/submicron cellulose in suspension, freezed-dried powder and hot-air dried powder are also examined. The aggregation in cellulose suspension is observed by optical microscopy. The surface properties of freezed-dried and hot-air dried powder are observed by SEM. The produced Nano/submicron cellulose by media milling was morphologically different from raw cellulose, and the particle size lied in nano/submicron scale is reconfirmed by laser diffraction particle size analyzer. The water-holding capacity of milled cellulose is found to be 8.5 times higher than raw cellulose, and also reaches consistency with the increase of specific surface area. As for safety assessment, the mutagenicity is evaluated by Ames test. The results show that there is no genotoxicity in Salmonella typhimurium TA98 and TA100. Cytotoxicity is also investigated in vitro. For portal-of-entry toxicity of skin and entero-mucosa, there is no significant cytotoxic effect on rat normal small intestinal cell line IEC-6, human skin fibroblast WS1 and human colon adenocarcinoma Caco-2 from nano/submicron cellulose. Furthermore, antimutagenicity of nano/submicron cellulose against 4-NQO is also considered. The data shows that there is no significant antimutagenicity in TA98 strain, but a slight antimutagenic reveals in TA100 strain. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T02:11:23Z (GMT). No. of bitstreams: 1 ntu-96-R94641013-1.pdf: 4235928 bytes, checksum: f6739dad3168d17a699eea6395d65654 (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 中文摘要………………………………………………………………………I
英文摘要……………………………………………………………………II 目錄…………………………………………………………………………III 表目錄……………………………………………………………………V 圖目錄………………………………………………………………………VI 壹、前言……………………………………………………………………01 1.1奈米科技……………………………………………………………01 1.2奈米材料的製備……………………………………………………03 1.3奈米食品………..………………………………………………04 1.4新穎性食品及其安全性評估………………………………………05 1.5奈米無機材料之安全性評估………………………………………10 1.6纖維素與膳食纖維……..………………………………………11 1.7實驗目的……………………………………………………………12 貳、材料與方法……………………………………………………………15 2.1材料及設備…………………………………………………………15 2.2奈米/次微米化纖維素製備………………………………………19 2.3粒徑分佈……………………………………………………………20 2.4形態觀察……………………………………………………………20 2.5奈米/次微米化纖維素保水力測定………..………………………21 2.6奈米/次微米化纖維素致突變性評估：安姆試驗（Ames test）……21 2.7奈米/次微米化纖維素之細胞毒性評估…………………………22 2.8奈米/次微米化纖維素抗致突變性測定…………………………24 2.9統計分析……………………………………………………………24 參、結果……………………………………………………………………25 3.1奈米/次微米化纖維素之粒徑分佈………………………………25 3.2形態觀察……………………………………………………….…26 3.3奈米/次微米化纖維素保水力測定………………………………29 3.4奈米/次微米化纖維素致突變性評估：安姆試驗（Ames test）……29 3.5奈米/次微米化纖維素之細胞毒性評估…………………………30 3.6奈米/次微米化纖維素抗致突變性測定…………..………………33 肆、討論……………………………………………………………………35 4.1奈米/次微米化纖維素之粒徑分佈以及熱穩定性…………….…35 4.2奈米/次微米化纖維素之形態上的變化…………………………36 4.3奈米/次微米化纖維素保水力的變化……………………………38 4.4奈米/次微米化纖維素的致突變性以及抗致突變性……………39 4.5奈米/次微米化纖維素之細胞毒性評估…………………………40 4.6奈米/次微米化纖維素未來之應用性………………………..……40 伍、結論……………………………………………………………………42陸、參考文獻………………………………………………………………43 | |
| dc.language.iso | zh-TW | |
| dc.subject | cytotoxicity | en |
| dc.subject | cellulose | en |
| dc.subject | genotoxicity | en |
| dc.subject | media-milling | en |
| dc.subject | nanotechnology | en |
| dc.title | 奈米/次微米化纖維素之特性及安全性 | zh_TW |
| dc.title | Characteristics and safety of nano/submicron cellulose | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 孫璐西,盧訓,蕭寧馨,賴鳳羲 | |
| dc.subject.keyword | 奈米,介質研磨,纖維素,保水力,細胞毒性,基因毒性, | zh_TW |
| dc.subject.keyword | nanotechnology,media-milling,cellulose,cytotoxicity,genotoxicity, | en |
| dc.relation.page | 82 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2007-06-25 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 食品科技研究所 | zh_TW |
| 顯示於系所單位： | 食品科技研究所 | |
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