台灣殼斗科植物之化學成分研究 (II)

Abstract

殼斗科椆屬植物赤柯、栲屬植物長尾尖葉櫧以及柯屬植物大葉石櫟為構成台灣山地中高海拔地區闊葉混合林之重要林種，本研究目的為調查以上三種植物葉部所含化學成分。三種植物之葉部乙醇萃取物分別以極性分割得到二氯甲烷可溶部分、正丁醇可溶部分和水可溶部分。其中正丁醇可溶部分以液相層析─質譜及液相層析─固相萃取─核磁共振串聯技術分析其成分，於赤柯葉部鑑定出13個黃酮類(12, 15-26)；於長尾尖葉櫧葉部鑑定出4個沒食子酸衍生物(28-31)、1個酚酸酯化物(27)、1個黃酮類(17)以及一含有castanopsinin系列化合物之組分；於大葉石櫟鑑定出3個黃酮類(12, 32, 33)。另外，赤柯葉部二氯甲烷可溶部分則經由管柱層析分離得到8個三萜類(1-8)、4個黃酮類(11-14)、1個單萜類(9)及1個酚類(10)。

Three Fagaceous plants, Cyclobalanopsis morii, Castanopsis cuspidata var. carlesii and Pasania kawakamii, are dominant tree species of the broad-leaved evergreen forests occurring at medium-high altitude in Taiwan. The study was aimed to investigate the chemical constituents of the leaves of these plants. The EtOH extract of the leaves was divided into fractions soluble in CH2Cl2, n-BuOH, and H2O. Application of LC-MS and LC-SPE-NMR hyphenated techniques in analyzing constituents in the n-BuOH layer led to the identification of 13 flavonoids (12, 15-26) in C. morii; four gallic acid derivatives (28-31), one phenolic acid ester (27), one flavonoid (17), and a castanopsinin-containing fraction in C. cuspidata var. carlesii; three flavonoids (12, 32, 33) in P. kawakamii. In addition, the CH2Cl2 layer of C. morii was chromatographed over Sephadex LH-20 and silica gel columns to yield eight triterpenoids (1-8), four flavonoids (11-14), one monoterpene (9), and one phenolic compound (10).