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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李水盛 | |
| dc.contributor.author | Ching-Chia Chang | en |
| dc.contributor.author | 張靜嘉 | zh_TW |
| dc.date.accessioned | 2021-06-13T01:17:00Z | - |
| dc.date.available | 2021-08-02 | |
| dc.date.copyright | 2011-10-07 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-03 | |
| dc.identifier.citation | 1. 鄭育斌, 台灣殼斗科植物的多樣性. 林業研究專訊 2009, 16, 11-14.
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Yoshihito, O., Ayako, O., Toru, O., A new triterpenoid isolated from Lagerstronemia speciosa (L.) PERS. Chem. Pharm. Bull. 2003, 51, 452-454 56. Lee, T. H., Juang, S. H., Hsu, F. L., Cheng,Y. W., Triterpene acids from the leaves of Planchonella duclitan (Blanco) Bakhuizan. J. Chin. Chem. Soc. -Taip. 2005, 52, 1275-1280 57. Lee, D. Y., Jung, L., Park, J. H., Yoo, K. H., Chung, I. S., Baek, N. I., Cytotoxic triterpenoids from Cornus kousa fruits. Chem. Nat. Compd. 2010, 46, 142-145 58. Chin, Y. W., Chai, H. B., Kinghorn, A. D., Keller, W. J. , Lignans and other constituents of the fruits of Euterpe oleracea (Acai) with antioxidant and cytoprotective activities. J. Agric. Food Chem. 2008, 56, 7759-7764 59. Nakabayashi, R., Kogure, N., Yamazaki, M., Kitajima, M., Saito, K., Takayama, H., Kusano, M., Kobayashi, M., Tohge, T., Metabolomics-oriented isolation and structure elucidation of 37 compounds including two anthocyanins from Arabidopsis thaliana. Phytochemistry 2009, 70, 1017-1029 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29741 | - |
| dc.description.abstract | 殼斗科椆屬植物赤柯、栲屬植物長尾尖葉櫧以及柯屬植物大葉石櫟為構成台灣山地中高海拔地區闊葉混合林之重要林種，本研究目的為調查以上三種植物葉部所含化學成分。三種植物之葉部乙醇萃取物分別以極性分割得到二氯甲烷可溶部分、正丁醇可溶部分和水可溶部分。其中正丁醇可溶部分以液相層析─質譜及液相層析─固相萃取─核磁共振串聯技術分析其成分，於赤柯葉部鑑定出13個黃酮類(12, 15-26)；於長尾尖葉櫧葉部鑑定出4個沒食子酸衍生物(28-31)、1個酚酸酯化物(27)、1個黃酮類(17)以及一含有castanopsinin系列化合物之組分；於大葉石櫟鑑定出3個黃酮類(12, 32, 33)。另外，赤柯葉部二氯甲烷可溶部分則經由管柱層析分離得到8個三萜類(1-8)、4個黃酮類(11-14)、1個單萜類(9)及1個酚類(10)。 | zh_TW |
| dc.description.abstract | Three Fagaceous plants, Cyclobalanopsis morii, Castanopsis cuspidata var. carlesii and Pasania kawakamii, are dominant tree species of the broad-leaved evergreen forests occurring at medium-high altitude in Taiwan. The study was aimed to investigate the chemical constituents of the leaves of these plants. The EtOH extract of the leaves was divided into fractions soluble in CH2Cl2, n-BuOH, and H2O. Application of LC-MS and LC-SPE-NMR hyphenated techniques in analyzing constituents in the n-BuOH layer led to the identification of 13 flavonoids (12, 15-26) in C. morii; four gallic acid derivatives (28-31), one phenolic acid ester (27), one flavonoid (17), and a castanopsinin-containing fraction in C. cuspidata var. carlesii; three flavonoids (12, 32, 33) in P. kawakamii. In addition, the CH2Cl2 layer of C. morii was chromatographed over Sephadex LH-20 and silica gel columns to yield eight triterpenoids (1-8), four flavonoids (11-14), one monoterpene (9), and one phenolic compound (10). | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T01:17:00Z (GMT). No. of bitstreams: 1 ntu-100-R98423001-1.pdf: 9615047 bytes, checksum: 4e8bee6079073208b5d53a4d2c4264ba (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 總目錄
口試委員會審定書...........................................i 誌謝......................................................ii 中文摘要.................................................iii Abstract..................................................iv 目錄.......................................................v 流程圖目錄(List of schemes).............................viii 表目錄(List of tables)..................................viii 圖目錄(List of figures).................................viii 辭彙(Glossary)..........................................xiii 目錄 第一章 緒論...............................................1 1.1 研究目的...............................................1 1.2赤柯之植物簡介..........................................2 1.3長尾尖葉櫧之植物簡介....................................3 1.4大葉石櫟之植物簡介......................................4 1.5 殼斗科椆屬、栲屬及柯屬植物成分之研究...................5 第二章 實驗結果與討論....................................13 2.1赤柯葉部成分之研究.....................................13 2.1.1三萜類化合物之結構解析...............................14 2.1.1.1 Lupane架構三萜類之結構解析........................14 2.1.1.1.1 3β-Hydroxy-lup-20(29)-en-28-oic acid (1).......14 2.1.1.1.2 2α,3β-Dihydroxy-lup-20(29)-en-28-oic acid (2).15 2.1.1.2 Oleanane架構三萜類之結構解析......................16 2.1.1.2.1 3β-Hydroxy-olean-12-en-28-oic acid (3).........16 2.1.1.2.2 3β-Hydroxy-1-oxo-olean-12-en-28-oic acid (4)...16 2.1.1.2.3 3β,23-Dihydroxy-1-oxo-olean-12-en-28-oic acid (5).......................................................16 2.1.1.3 Ursane架構三萜類之結構解析........................21 2.1.1.3.1 3β,19α-Dihydroxy-urs-12-en-28-oic acid (6)....21 2.1.1.3.2 2α,3α,19α-Trihydroxyurs-12-en-28-oic acid(7).21 2.1.1.4 Lanostane架構三萜類之結構解析.....................22 2.1.1.4.1 3β,22(S),23(S)-Trihydroxy-cycloart-24-ene (8).22 2.1.2 黃酮類化合物之結構解析..............................26 2.1.2.1 Quercetin (11)及quercetin 3-O-α-L-rhamnopyranoside (12)......................................................27 2.1.2.2 Kaempferol (13)...................................27 2.1.2.3 Chrysoeriol (14)..................................28 2.1.2.4 LC-MS分析.........................................31 2.1.2.5 LC-SPE-NMR分析....................................32 2.1.2.5.1 Quercetin 3-O-β-D-glucopyranoside (16).........35 2.1.2.5.2 Kaempferol 3-O-α-L-rhamnopyranoside (17),......35 Kaempferol 3-O-β-D-galactopyranoside (18),...............35 Kaempferol 3-O-β-D-glucopyranoside (20)..................35 2.1.2.5.3 Isorhamnetin 3-O-β-D-galactopyranoside (19),.....................................................36Isorhamnetin 3-O-β-D-glucopyranoside (21),...............36 Isorhamnetin 3-O-α-L-rhamnopyranoside (26)...............36 2.1.2.5.4 Apigenin 7-O-β-D-glucopyranoside (22)..........37 2.1.2.5.5 Chrysoeriol 7-O-β-D-glucopyranoside (23).......37 2.1.2.5.6 Diosmetin 7-O-β-D-glucopyranoside (24).........38 2.1.2.5.7 Diosmetin 3'-O-β-D-glucopyranoside (25)........38 2.2 長尾尖葉櫧葉部成分之研究..............................39 2.2.1 LC-MS分析...........................................39 2.2.2 LC-SPE-NMR分析......................................40 2.2.2.1 Chlorgenic acid methyl ester (27).................42 2.2.2.2 3,3'-Di-O-methylellagic acid (31),................43 3,3'-Di-O-methylellagic acid-4-α-L-arabinofuranoside (29),.....................................................43 3,3'-Di-O-methylellagic acid-4-(5'-acetyl)-α-L-arabinofuranoside (30)..................................43 2.2.3 長尾尖葉櫧castanopsinin-containing fraction之LC-MS/MS分析......................................................45 2.3 大葉石櫟葉部成分之研究................................46 2.3.1 LC-MS分析...........................................47 2.4 結論..................................................48 第三章 實驗方法..........................................49 3.1 儀器與材料............................................49 3.1.1 理化性質測定儀器....................................49 3.1.2 成分分離及分析之儀器及材料.........................49 3.1.3 試藥及溶劑..........................................50 3.2 赤柯葉部成分之萃取與分離..............................51 3.2.1二氯甲烷可溶部分之分離...............................51 3.2.1.1 化合物1-10之分離..................................51 3.2.1.2 化合物11-14之分離.................................52 3.2.2 赤柯正丁醇可溶部分之分離............................52 3.2.2.1 赤柯正丁醇可溶部分sub-fr.I-3, II-2及II-3之LC-MS分析........................................................52 3.2.2.2 以HPLC比對赤柯正丁醇可溶部分sub-fr.I-3之成分(12,15-17)................................................55 3.2.2.3 赤柯正丁醇可溶部分sub-fr.II-2之LC-SPE-NMR分析.....56 3.3 長尾尖葉櫧葉部成分之抽取與分離........................56 3.3.1 長尾尖葉櫧正丁醇可溶部分之分離......................56 3.3.1.1 長尾尖葉櫧正丁醇可溶部分次組分I-2之LC-MS分析......57 3.3.1.2 長尾尖葉櫧正丁醇可溶部分次組分I-2之LC-SPE-NMR分析.57 3.3.1.2.1 化合物17, 27-31之HR-ESI-MS分析..................58 3.3.1.3 長尾尖葉櫧正丁醇可溶部分castanopsinin-containing fraction之LC-MS/MS分析....................................58 3.4 大葉石櫟葉部成分之抽取與分離..........................58 3.4.1 大葉石櫟正丁醇可溶部分次組分2之LC-MS分析............59 3.4.2 以HPLC比對大葉石櫟正丁醇可溶部分sub-fr.2之成分(12,32,33)................................................59 3.5 各化合物之物理數據....................................65 3.5.1 化合物1-14為分離純化得到............................65 3.5.2 化合物16-31為運用LC-SPE-NMR串聯技術鑑定.............68 3.5.3 化合物15,32,33為運用HPLC分析方法鑑定................71 參考文獻..................................................72 附圖......................................................77 | |
| dc.language.iso | zh-TW | |
| dc.subject | 大葉石櫟 | zh_TW |
| dc.subject | 赤柯 | zh_TW |
| dc.subject | 殼斗科 | zh_TW |
| dc.subject | 長尾尖葉櫧 | zh_TW |
| dc.subject | 沒食子酸衍生物 | zh_TW |
| dc.subject | 三萜 | zh_TW |
| dc.subject | 類 | zh_TW |
| dc.subject | 黃酮類 | zh_TW |
| dc.subject | Cyclobalanopsis morii | en |
| dc.subject | triterpenoids | en |
| dc.subject | gallic acid derivatives | en |
| dc.subject | flavonoids | en |
| dc.subject | Pasania kawakamii | en |
| dc.subject | Fagaceous | en |
| dc.subject | Castanopsis cuspidata var. carlesii | en |
| dc.title | 台灣殼斗科植物之化學成分研究 (II) | zh_TW |
| dc.title | Chemical Constituents of Fagaceous Plants in Taiwan (II) | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳繼明,林雲蓮,陳春雄 | |
| dc.subject.keyword | 殼斗科,赤柯,長尾尖葉櫧,大葉石櫟,黃酮類,沒食子酸衍生物,三萜,類, | zh_TW |
| dc.subject.keyword | Fagaceous,Cyclobalanopsis morii,Castanopsis cuspidata var. carlesii,Pasania kawakamii,flavonoids,gallic acid derivatives,triterpenoids, | en |
| dc.relation.page | 141 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-08-03 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 藥學研究所 | zh_TW |
| 顯示於系所單位： | 藥學系 | |
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| ntu-100-1.pdf 未授權公開取用 | 9.39 MB | Adobe PDF |
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