以硫酸鹽衍生化環糊精為對掌選擇劑對羥基黃烷酮對 掌異構物之分離與研究

Abstract

本篇論文利用毛細管區帶電泳法針對三種羥基黃烷酮，於反向電壓、低pH值下，添加硫酸鹽衍生化環糊精(MI-S-b-CD)來進行分離的研究並且添加十二烷基硫酸鈉鹽(SDS)單體當做錯合劑，來控制羥基黃烷酮的選擇性，並且還利用雙環糊精系統作為為對掌選擇劑。結果指出硫酸鹽衍生化環糊精對於羥基黃烷酮來說是一種很好的對掌選擇劑，尤其是2`-羥基黃烷酮。另一方面，利用雙環糊精系統(dualCD systems)來分離羥基黃烷酮，可大大提升對掌選擇性。我們利用2%的硫酸鹽衍生化環糊精或是添加低於2 mM 的十二烷基硫酸鈉鹽單體於雙環糊精系統下，可有效地使三種羥基黃烷酮異構物在20 分鐘內達到對掌分離的效果。

In this study, enantioseparations of three hydroxyflavanone aglycones, including 2’-，3’- and 4’-hydroxyflavanone, in cyclodextrin (CD)-modified capillary zone electrohphoresis using randomly sulfate-substituted b-CD (MI-S-b-CD) or dual CD systems consisting of MI-S-b-CD and neutral CDs such as b-CD or g-CD as chiral selectors were investigated with a phosphate buffer at low pH. Sodium dodecyl sulfate (SDS) monomers were used as complexing agents for manipulating the selectivity of hydroxyflavanones. The results indicate that MI-S-b-CD is an excellent chiral selector for enantioseparation of hydroxyflavanone aglycones, especially for 2’-hydroxyflavanone. The enantioselectivity of hydroxyflavanones can be greatly enhanced using dual CD systems. Effective enantioseparations of these three hydroxyflavanones can be simultaneously achieved within 20 minutes with 2.0 % (w/v) MI-S-b-CD or dual CD systems in combination with the addition of SDS monomers at a concentration less than 2 mM.