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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林萬寅(Wann-Yin Lin) | |
| dc.contributor.author | Wei-Ru Fang | en |
| dc.contributor.author | 方巍儒 | zh_TW |
| dc.date.accessioned | 2021-06-13T01:14:57Z | - |
| dc.date.available | 2007-07-24 | |
| dc.date.copyright | 2007-07-24 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-07-19 | |
| dc.identifier.citation | 第一章參考文獻
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29688 | - |
| dc.description.abstract | 本篇論文利用毛細管區帶電泳法針對三種羥基黃烷酮，於反向電壓、低pH值下，添加硫酸鹽衍生化環糊精(MI-S-b-CD)來進行分離的研究並且添加十二烷基硫酸鈉鹽(SDS)單體當做錯合劑，來控制羥基黃烷酮的選擇性，並且還利用雙環糊精系統作為為對掌選擇劑。結果指出硫酸鹽衍生化環糊精對於羥基黃烷酮來說是一種很好的對掌選擇劑，尤其是2`-羥基黃烷酮。另一方面，利用雙環糊精系統(dualCD systems)來分離羥基黃烷酮，可大大提升對掌選擇性。我們利用2%的硫酸鹽衍生化環糊精或是添加低於2 mM 的十二烷基硫酸鈉鹽單體於雙環糊精系統下，可有效地使三種羥基黃烷酮異構物在20 分鐘內達到對掌分離的效果。 | zh_TW |
| dc.description.abstract | In this study, enantioseparations of three hydroxyflavanone aglycones, including
2’-，3’- and 4’-hydroxyflavanone, in cyclodextrin (CD)-modified capillary zone electrohphoresis using randomly sulfate-substituted b-CD (MI-S-b-CD) or dual CD systems consisting of MI-S-b-CD and neutral CDs such as b-CD or g-CD as chiral selectors were investigated with a phosphate buffer at low pH. Sodium dodecyl sulfate (SDS) monomers were used as complexing agents for manipulating the selectivity of hydroxyflavanones. The results indicate that MI-S-b-CD is an excellent chiral selector for enantioseparation of hydroxyflavanone aglycones, especially for 2’-hydroxyflavanone. The enantioselectivity of hydroxyflavanones can be greatly enhanced using dual CD systems. Effective enantioseparations of these three hydroxyflavanones can be simultaneously achieved within 20 minutes with 2.0 % (w/v) MI-S-b-CD or dual CD systems in combination with the addition of SDS monomers at a concentration less than 2 mM. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T01:14:57Z (GMT). No. of bitstreams: 1 ntu-96-R94223081-1.pdf: 2052457 bytes, checksum: ea6a315eda6f6308b019f67c163c60a2 (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 目錄
謝誌………………………………………………………………………. i 中文摘要……………………………………………………………… ii 英文摘要………………………………………………………………iii 第一章 1 緒論 1 1.1毛細管電泳的發展史-------------------------------------1 1.1.1電泳-------------------------------------------------1 1.1.2毛細管電泳-------------------------------------------2 1.2毛細管電泳的分離原理-----------------------------------3 1.2.1荷電離子在電泳的遷移行為-----------------------------3 1.2.2電滲流與荷電分子的遷移行為---------------------------4 1.2.3分離效率---------------------------------------------6 1.3毛細管電泳的分離模式-----------------------------------7 1.4毛細管電泳法在對掌異構物分離的應用---------------------8 1.4.1環糊精性質簡介------------------------------------- 10 1.4.2環糊精對對掌異構物分離的原理----------------------- 11 1.5毛細管電泳儀的裝置----------------------------------- 12 1.6毛細管電泳的優點與發展趨勢--------------------------- 12 1.7研究動機--------------------------------------------- 13 1.8參考文獻--------------------------------------------- 14 第二章 24 以硫酸鹽衍生化環糊精為對掌選擇劑對羥基黃烷酮對掌異構 物之分離之研究------------------------------------------ 24 2.1前言------------------------------------------------- 24 2.2實驗------------------------------------------------- 26 2.2.1儀器----------------------------------------------- 26 2.2.1.1毛細管電泳儀------------------------------------- 26 2.2.1.2酸鹼度測定儀------------------------------------- 26 2.2.1.3毛細管柱----------------------------------------- 27 2.2.2藥品----------------------------------------------- 27 2.2.2.1分析物------------------------------------------- 27 2.2.2.2緩衝溶液----------------------------------------- 27 2.2.2.3緩衝溶液添加劑----------------------------------- 27 2.2.2.4其他試藥----------------------------------------- 28 2.2.3實驗方法------------------------------------------- 28 2.2.3.1毛細管的處理------------------------------------- 28 2.2.3.2標準樣品的配製----------------------------------- 28 2.2.3.3緩衝溶液及添加劑的配製--------------------------- 28 2.2.3.4實驗操作條件------------------------------------- 29 2.2.4電泳遷移率計算公式--------------------------------- 29 2.2.5對掌選擇率計算公式--------------------------------- 30 2.3結果與討論------------------------------------------- 30 2.3.1三種hydroxyflavanones 立體結構上的差異------------- 30 2.3.2在反向電壓極性下以 MI-S-b-CD為對掌選擇劑----------- 32 2.3.3 在MI-S-b-CD存在下以SDS單體為錯合劑---------------- 33 2.3.4 MI-S-b-CD/天然環糊精的雙環糊精系統為對掌選擇劑---- 34 2.3.5 添加SDS 於雙環糊精系統---------------------------- 35 2.4 結論------------------------------------------------ 37 2.5 本章參考文獻---------------------------------------- 38 圖目錄 圖 1-1: 毛細管內矽羥基的解離情況......................................................19 圖1- 2: 毛細管內電雙層模型.......................................................19 圖1- 3: HPLC 與CE 的液體流動的型態.......................................................20 圖 1-4 : 毛細管電泳儀裝置示意圖.......................................................21 圖 1-5 : 環糊精的結構圖.......................................................22 表 1-1: 環糊精性質表.......................................................23 圖2-1: 緩衝溶液中(pH 9.3)添加-CD試劑對2’-hydroxyflavanone分離的電泳圖。.....................................................42 圖2-2: 緩衝溶液中(pH 9.3)添加-CD試劑濃度對4’-hydroxyflavanone分離的電泳圖。.....................................................43 圖2-3: 在反向電壓極性下，添加不同濃度的MI-S-b-CD。.....................................................44 圖2-4: 在反向電壓極性下，2 mM SDS 存在下，添加不同濃度的 MI-S-b-CD。.....................................................45 圖2-5: 在反向電壓極性下，3 mM SDS 存在下，添加不同濃度的 MI-S-b-CD。.....................................................46 圖2-6: 2`-、3`- 和 4`-hydroxyflavanone 三種分析物在反向電壓極性 下，添加2% MI-S-b-CD和 2 mM b-CD的分離電泳圖。...........47 圖2-7: 在反向電壓極性下，添加 g-CD/MI-S-g-CD 混合試劑對分析物分離的電泳圖。.....................................................48 圖2-8: 3`-和 4`-hydroxyflavanone 在反向電壓極性下，2 mM SDS存 在下，添加 b-CD/MI-S-b-CD 混合試劑對分析物分離的電泳 圖。.....................................................49 圖2-9: 2`-和 4`-hydroxyflavanone 在反向電壓極性下，2 mM SDS存在下，添加 b-CD/MI-S-b-CD 混合試劑對分析物分離的電泳 圖。.....................................................50 圖2-10: 2`-、3`- 和 4`-hydroxyflavanone三種分析物在2 mM SDS 存在下，添加2% MI-S-b-CD和 2 mM b-CD在反向電壓極性下 的分離電泳圖。.....................................................51 圖2-11: 3`- 和 4`-hydroxyflavanone 在反向電壓極性下，2 mM SDS存在下，添加 g-CD/MI-S-b-CD 混合試劑對分析物分離的電泳 圖。.....................................................52 圖2-12: 2`-和 4`-hydroxyflavanone 在反向電壓極性下，2 mM SDS存在下，添加 g-CD/MI-S-b-CD 混合試劑對分析物分離的電泳 圖。.....................................................53 圖2-13: 2`-、3`- 和 4`-hydroxyflavanone三種分析物在2 mM SDS 存在下，添加 g-CD/MI-S-b-CD 混合試劑對分析物分離的電泳 圖。.....................................................54 表2-1 三種分析物結構及資料圖。.....................................................55 表2-2 2`和3`-hydroxyflavanone 在pH=3的磷酸鹽溶液下，添加不同濃度MI-S-b-CD的解析度(resolution) 和對掌選擇率 (enantioselectivity)數據圖表。.....................................................56 表 2-3 2 mMSDS與2% MI-S-b-CD 存在下添加 b-CD對掌選擇性的 數據:......................................................57 表2-4 2 mM SDS 與2% MI-S-b-CD 存在下添加 g-CD對掌選擇性的 數據.....................................................58 | |
| dc.language.iso | zh-TW | |
| dc.subject | 對掌分離 | zh_TW |
| dc.subject | 毛細管電泳 | zh_TW |
| dc.subject | 環糊精 | zh_TW |
| dc.subject | 類黃酮 | zh_TW |
| dc.subject | 黃烷酮 | zh_TW |
| dc.subject | flavanone | en |
| dc.subject | Capillary Zone Electrophoresis | en |
| dc.subject | chiral seperation | en |
| dc.subject | Cyclodextrins | en |
| dc.subject | flavnoids | en |
| dc.title | 以硫酸鹽衍生化環糊精為對掌選擇劑對羥基黃烷酮對
掌異構物之分離與研究 | zh_TW |
| dc.title | Enantioseparation of Hydroxyflavanones in
Cyclodextrin-Modified Capillary Zone Electrophoresis Using Sulfated Cyclodextrins as Chiral Selectors | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 林敬二(Ching-Erh Lin) | |
| dc.contributor.oralexamcommittee | 林震煌(Cheng-Huang Lin) | |
| dc.subject.keyword | 毛細管電泳,對掌分離,環糊精,類黃酮,黃烷酮, | zh_TW |
| dc.subject.keyword | Capillary Zone Electrophoresis,chiral seperation,Cyclodextrins,flavnoids,flavanone, | en |
| dc.relation.page | 58 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2007-07-20 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 化學研究所 | zh_TW |
| 顯示於系所單位： | 化學系 | |
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