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標題: | Wnt/β-catenin訊息傳遞鏈對Aeolosoma viride前端再生調控之探討 Wnt/β-catenin Signaling Pathway Regulates Anterior regeneration in Aeolosoma viride |
作者: | Cheng-Yi Chen 陳政儀 |
指導教授: | 陳俊宏(Jiun-Hong Chen) |
關鍵字: | 環節動物,再生,wnt 細胞分子傳遞鏈, Annelids,regeneration,wnt signaling pathway, |
出版年 : | 2011 |
學位: | 碩士 |
摘要: | 不僅是水螅及渦蟲,wnt/β-catenin細胞訊息傳遞鏈已被證實調控大多數生物的再生行為。環節動物是眾所皆知具有很強再生能力的一群動物,然而科學界並未有關於此訊息傳遞鏈與其再生之探討。本文使用淡水生環節動物Aeolosoma viride做為實驗對象進行研究。Wnt促效劑(wnt agonist)與另外兩種wnt/β-catenin訊息傳遞鏈促進物(alsterpaullone(ALP)及LiCl)會抑制A. viride的前端再生,其原因可能來自於細胞增生或神經再生受到抑制。於不同再生時間點以ALP處理再生個體,發現再生早期必須要將此訊息傳遞鏈關閉。雖然於再生同時以quercetin或XAV939(Wnt/β-catenin訊息傳遞鏈抑制物)處理並不會影響再生成功率,但可以逆轉wnt促效劑對再生的抑制。且前端切除前先以quercetin或XAV939的組別會有較高的再生成功率。免疫染色結果則顯示Av-β-catenin及c-Jun這兩個wnt/β-catenin訊息傳遞鏈相關蛋白質,會隨著再生過程進行而有變化。根據以上結果可以推論:由於促進wnt/β-catenin訊息傳遞鏈會抑制再生時不可或缺的細胞增生,因此wnt/β-catenin訊息傳遞鏈於A. viride再生早期必須被關閉,否則將無法正常再生。 Wnt/β-catenin pathway has been proven to regulate regeneration in hydra, planarian and several vertebrates. Annelids also possess strong regenerative ability, though little is known about whether or not wnt/β-catenin pathway participates in these animals’ regeneration. Herein, the freshwater annelid, Aeolosoma viride, was used as the experimental animal in this study. The results showed that wnt agonist, alsterpaullone (ALP) and LiCl, which can inhibit GSK-3β and in turn activate the pathway, significantly inhibited amputees’ anterior regeneration, which may be resulted from cell proliferation and nerve repair defects. Treatments of ALP during different regeneration periods showed the pathway exerts its effect in early phases of regeneration. On the other hand, quercetin and XAV939, inhibitors of this pathway, partially rescued the inhibitory effect of wnt agonist on regeneration and helped the regeneration process when worms were pre-treated before amputation. The immunoblotting results showed that the amount of one of the two putative Av-β-catenin proteins was increased as A. viride gradually repaired, and that c-Jun, one of wnt/β-catenin pathway downstream genes, was found to be down-regulated during A. viride regeneration. These results suggest that wnt/β-catenin pathway is indeed involved into regeneration, and it should be turned down early for proper anterior regeneration in A. viride. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29452 |
全文授權: | 有償授權 |
顯示於系所單位: | 動物學研究所 |
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