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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 陳俊宏(Jiun-Hong Chen) | |
dc.contributor.author | Cheng-Yi Chen | en |
dc.contributor.author | 陳政儀 | zh_TW |
dc.date.accessioned | 2021-06-13T01:07:28Z | - |
dc.date.available | 2013-08-10 | |
dc.date.copyright | 2011-08-10 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-04 | |
dc.identifier.citation | Ameyar, M., M.Wisniewska and J.B.Weitzman. (2003). A role for AP-1 in apoptosis: the case for and against. Biochimie. 85.
Augustin, R., Franke, A., Khalturin, K., Kiko, R., Siebert, S., Hemmrich, G. and Bosch, T. C. G. (2006). Dickkopf related genes are components of the positional value gradient in Hydra. Developmental Biology 296, 62-70. Bely, A. E. and Sikes, J. M. (2009). Latent regeneration abilities persist following recent evolutionary loss in asexual annelids. Proceedings of the National Academy of Sciences 107, 1464-1469. Bely, A. E. and Wray, G. A. (2001). Evolution of regeneration and fission in annelids: insights from engrailedand orthodenticle-class gene expression. Development 128. Bergmann, A. and Steller, H. (2010). Apoptosis, stem cells, and tissue regeneration. Science 3. Broun, M., Gee, L., Reinhardt, B. and Bode, H. R. (2005). Formation of the head organizer in hydra involves the canonical Wnt pathway. Development 132, 2907-2916. Cabrera, C. V., Alonso, M. C., Johnston, P., Phillips, R. G. and Lawrence, P. A. (1987). Phenocopies Induced with Antisense RNA Identify the wingless Gene. Cell 50. Cebria, F. and Newmark, P. A. (2005). Planarian homologs of netrin and netrin receptor are required for proper regeneration of the central nervous system and the maintenance of nervous system architecture. Development 132, 3691-3703. Cebria, F. and Newmark, P. A. (2007). Morphogenesis defects are associated with abnormal nervous system regeneration following roboA RNAi in planarians. Development 134, 833-837. Chera, S., Ghila, L., Dobretz, K., Wenger, Y., Bauer, C., Buzgariu, W., Martinou, J.-C. and Galliot, B. (2009). Apoptotic Cells Provide an Unexpected Source of Wnt3 Signaling to Drive Hydra Head Regeneration. Developmental Cell 17, 279-289. Ferguson, M. W. J. and O’Kane, S. (2004). Scar-free healing: from embryonic mechanisms to adult therapeutic intervention. Philos Trans R Soc Lond B Biol Sci. 359. Gotschela, F., Kernb, C., Langb, S., Sparnab, T., Markmannb, C., Schwagerc, J., McNellyd, S., Weizsackerd, F. v., Laufere, S., Hechta, A. et al. (2008). Inhibition of GSK3 differentially modulates NF-kappaB, CREB, AP-1 and beta-catenin signaling in hepatocytes, but fails to promote TNF-alpha-induced apoptosis. Exp Cell Res. 314. Galliot, B. and Chera, S. (2010). The Hydra model: disclosing an apoptosis-driven generator of Wnt-based regeneration. Trends in Cell Biology 20, 514-523. Gilbert, S. F. (2009). Developmental Biology, Eighth Edition. Sunderland, MA: Sinauer Associates, Inc., Publishers, 283 and 319. Grotewold L, R. U. (2002). The Wnt antagonist Dickkopf-1 is regulated by Bmp signaling and c-Jun and modulates programmed cell death. EMBO J. 21. Gurley, K. A., Rink, J. C. and Alvarado, A. S. (2008). Beta-Catenin Defines Head Versus Tail Identity During Planarian Regeneration and Homeostasis. Science 319, 323-327. Hessling, R. and Purschke, G. (2000). Immunohistochemical (cLSM) and ultrastructural analysis of the central nervous system and sense organs in Aeolosoma hemprichi (Annelida, Aeolosomatidae). Zoomorphology 120. Huang, S.-M. A., Mishina, Y. M., Liu, S., Cheung, A., Stegmeier, F., Michaud, G. A., Charlat, O., Wiellette, E., Zhang, Y., Wiessner, S. et al. (2009). Tankyrase inhibition stabilizes axin and antagonizes Wnt signalling. Nature 461, 614-620. Iglesias, M., Gomez-Skarmeta, J. L., Salo, E. and Adell, T. (2008). Silencing of Smed-Beta catenin1 generates radial-like hypercephalized planarians. Development 135, 1215-1221. Jiang, X., Tan, J., Li, J., Kivimae, S., Yang, X., Zhuang, L., Lee, P. L., Chan, M. T. W., Stanton, L. W. and Liu, E. T. (2008). DACT3 Is an Epigenetic Regulator of Wnt/β-Catenin Signaling in Colorectal Cancer and Is a Therapeutic Target of Histone Modifications. Cancer Cell 13, 529-541. Katoh, M. and Katoh, M. (2007). WNT Signaling Pathway and Stem Cell Signaling Network. Clinical Cancer Research 13, 4042-4045. Kawakami, Y., Rodriguez Esteban, C., Raya, M., Kawakami, H., Marti, M., Dubova, I. and Izpisua Belmonte, J. C. (2006). Wnt/beta-catenin signaling regulates vertebrate limb regeneration. Genes & Development 20, 3232-3237. Kawamoto, S., Yoshida-Noro, C. and Tochinai, S. (2005). Bipolar head regeneration induced by artificial amputation in Enchytraeus japonensis (Annelida, Oligochaeta). Journal of Experimental Zoology Part A: Comparative Experimental Biology 303A, 615-627. Kiecker, C. and Niehrs, C. (2001). A morphogen gradient of Wnt/β-catenin signalling regulates anteroposterior neural patterning in Xenopus. Development 128, 4189-4201. Lengfeld, T., Watanabe, H., Simakov, O., Lindgens, D., Gee, L., Law, L., Schmidt, H. A., Ozbek, S., Bode, H. and Holstein, T. W. (2009). Multiple Wnts are involved in Hydra organizer formation and regeneration. Developmental Biology 330, 186-199. Liu C, L. Y., Semenov M, Han C, Baeg GH, Tan Y, Zhang Z, Lin X, He X. (2002). Control of beta-catenin phosphorylation/degradation by a dual-kinase mechanism. Cell 108. Liu, J., Wu, X., Mitchell, B., Kintner, C., Ding, S. and Schultz, P. G. (2005). A Small-Molecule Agonist of the Wnt Signaling Pathway. Angewandte Chemie International Edition 44, 1987-1990. Logan, C. Y., Miller, J. R., Ferkowicz, M. J. and McClay, D. R. (1999). Nuclear β-catenin is required to specify vegetal cell fates in the sea urchin embryo. Development 126. Muller, M. C. M. (2004). Nerve development, growth and differentiation during regeneration in Enchytraeus fragmentosus and Stylaria lacustris (Oligochaeta). Develop. Growth Differ. 46. Muller, M. C. M., Berenzen, A. and Westheide, W. (2003). Experiments on anterior regeneration in Eurythoe complanata (“Polychaeta”, Amphinomidae): reconfiguration of the nervous system and its function for regeneration. Zoomorphology 122, 95-103. Martinez, V., Mengeriii, G. and Zoran, M. (2005). Regeneration and asexual reproduction share common molecular changes: upregulation of a neural glycoepitope during morphallaxis in. Mechanisms of Development 122, 721-732. Mendes, C. T., Mury, F. B., Sa Moreira, E., Alberto, F. L., Forlenza, O. V., Dias-Neto, E. and Gattaz, W. F. (2008). Lithium reduces Gsk3b mRNA levels: implications for Alzheimer Disease. European Archives of Psychiatry and Clinical Neuroscience 259, 16-22. Menger, B., Vogt, P. M., Kuhbier, J. W., Med, C. and Kerstin Reimers. (2010). Applying amphibian limb regeneration to human wound healing: a review. Ann Plast Surg. 65. Molina, M. D., Salo, E. and Cebria, F. (2007). The BMP pathway is essential for re-specification and maintenance of the dorsoventral axis in regenerating and intact planarians. Developmental Biology 311, 79-94. Myohara, M. (2004). Differential tissue development during embryogenesis and regeneration in an annelid. DEVELOPMENTAL DYNAMICS 231, 349-358. Newmark, P. A. and Alvarado, A. S. n. (2000). Bromodeoxyuridine Specifically Labels the Regenerative Stem Cells of Planarians. Developmental Biology 220, 142-153. Park, C., Chang, J., Hahm, E., Park, S., Kim, H. and Yang, C. (2005). Quercetin, a potent inhibitor against β-catenin/Tcf signaling in SW480 colon cancer cells. Biochemical and Biophysical Research Communications 328, 227-234. Parka, C. H., Changa, J. Y., Hahma, E. R., Parkb, S., Kima, H.-K. and Yanga, C. H. (2009). Quercetin Suppresses Early Colon Carcinogenesis Partly through Inhibition of Inflammatory Mediators. Acta horticulturae 841. Paulus, T. and Muller, M. C. M. (2006). Cell proliferation dynamics and morphological differentiation during regeneration in Dorvillea bermudensis (Polychaeta, Dorvilleidae). JOURNAL OF MORPHOLOGY 267, 393-403. Petersen, C. P. and Reddien, P. W. (2008). Smed-Beta-catenin-1 Is Required for Anteroposterior Blastema Polarity in Planarian Regeneration. Science 319, 327-330. Rijsewijk, F., Schuermann, M., Wagenaar, E., Parren, P., Weigel, D. and Nusse, R. (1987). The Drosophila Homolog of the Mouse Mammary Oncogene int-1 Is Identical to the Segment Polarity Gene wingless. Cell 50. Rink, J. C., Gurley, K. A., Elliott, S. A. and Sanchez Alvarado, A. (2009). Planarian Hh Signaling Regulates Regeneration Polarity and Links Hh Pathway Evolution to Cilia. Science 326, 1406-1410. Schultz, C., Link, A., Leost, M., Zaharevitz, D. W., Gussio, R., Sausville, E. A., Meijer, L. and Kunick, C. (1999). Paullones, a Series of Cyclin-Dependent Kinase Inhibitors: Synthesis, Evaluation of CDK1/Cyclin B Inhibition, and in Vitro Antitumor Activity. J. Med. Chem. 42. Tadokoro, R., Sugio, M., Kutsuna, J., Tochinai, S. and Takahashi, Y. (2006). Early Segregation of Germ and Somatic Lineages during Gonadal Regeneration in the Annelid Enchytraeus japonensis. Current Biology 16, 1012-1017. Tanaka, E. M. and Weidinger, G. (2008). Heads or tails: can Wnt tell which one is up? Nature 10. Yokoyama, H., Ogino, H., Stoick-Cooper, C. L., Grainger, R. M. and Moon, R. T. (2007). Wnt/beta-catenin signaling has an essential role in the initiation of limb regeneration. Dev Biol. 306, 170-178. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29452 | - |
dc.description.abstract | 不僅是水螅及渦蟲,wnt/β-catenin細胞訊息傳遞鏈已被證實調控大多數生物的再生行為。環節動物是眾所皆知具有很強再生能力的一群動物,然而科學界並未有關於此訊息傳遞鏈與其再生之探討。本文使用淡水生環節動物Aeolosoma viride做為實驗對象進行研究。Wnt促效劑(wnt agonist)與另外兩種wnt/β-catenin訊息傳遞鏈促進物(alsterpaullone(ALP)及LiCl)會抑制A. viride的前端再生,其原因可能來自於細胞增生或神經再生受到抑制。於不同再生時間點以ALP處理再生個體,發現再生早期必須要將此訊息傳遞鏈關閉。雖然於再生同時以quercetin或XAV939(Wnt/β-catenin訊息傳遞鏈抑制物)處理並不會影響再生成功率,但可以逆轉wnt促效劑對再生的抑制。且前端切除前先以quercetin或XAV939的組別會有較高的再生成功率。免疫染色結果則顯示Av-β-catenin及c-Jun這兩個wnt/β-catenin訊息傳遞鏈相關蛋白質,會隨著再生過程進行而有變化。根據以上結果可以推論:由於促進wnt/β-catenin訊息傳遞鏈會抑制再生時不可或缺的細胞增生,因此wnt/β-catenin訊息傳遞鏈於A. viride再生早期必須被關閉,否則將無法正常再生。 | zh_TW |
dc.description.abstract | Wnt/β-catenin pathway has been proven to regulate regeneration in hydra, planarian and several vertebrates. Annelids also possess strong regenerative ability, though little is known about whether or not wnt/β-catenin pathway participates in these animals’ regeneration. Herein, the freshwater annelid, Aeolosoma viride, was used as the experimental animal in this study. The results showed that wnt agonist, alsterpaullone (ALP) and LiCl, which can inhibit GSK-3β and in turn activate the pathway, significantly inhibited amputees’ anterior regeneration, which may be resulted from cell proliferation and nerve repair defects. Treatments of ALP during different regeneration periods showed the pathway exerts its effect in early phases of regeneration. On the other hand, quercetin and XAV939, inhibitors of this pathway, partially rescued the inhibitory effect of wnt agonist on regeneration and helped the regeneration process when worms were pre-treated before amputation. The immunoblotting results showed that the amount of one of the two putative Av-β-catenin proteins was increased as A. viride gradually repaired, and that c-Jun, one of wnt/β-catenin pathway downstream genes, was found to be down-regulated during A. viride regeneration. These results suggest that wnt/β-catenin pathway is indeed involved into regeneration, and it should be turned down early for proper anterior regeneration in A. viride. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T01:07:28Z (GMT). No. of bitstreams: 1 ntu-100-R98b41008-1.pdf: 7704229 bytes, checksum: a8acd5d096f000169f14d8b3ca3aab10 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 中文摘要 i
Abstract ii Introduction 1 Introduction of Wnt/β-catenin signaling pathway 1 Participation of wnt/β-catenin pathway in regeneration 3 Comparison of regeneration among different animal phyla 5 Introduction of Aeolosoma viride 7 Aim and significance of this study 8 Materials and methods 9 Aeolosoma viride 9 Head amputation 9 Treatments during anterior regeneration 9 mRNA extraction 10 Reverse transcription 11 Cloning of Av-Gsk-3β and Av-β-catenin 11 BrdU labeling for proliferating cell 13 Whole-mount immunostaining 14 SDS-PAGE and immunoblotting analysis 15 Expression of recombinant Av-β-catenin 16 Mouse antiserum against Av-β-catenin 17 Statistical analysis 18 Results 19 Description of Aeolosoma viride 19 The effect of wnt agonist on regeneration and blastema formation 20 The requirement of GSK-3β during regeneration 21 Nervous system defects after the inhibition of GSK-3β 21 The activity of GSK-3β in different phases of regeneration 22 The effect of exogenous wnt inhibitors, quercetin and XAV939, on anterior regeneration 22 Av-β-catenin expression during anterior regeneration 24 c-Jun expression fluctuated during anterior regeneration 24 Discussion 26 The activity of GSK-3β is required for A. viride regeneratin 26 Wnt/β-catenin pathway should be inhibited at early regeneration phase 26 Inhibiting wnt/β-catenin pathway and anterior regeneration 27 Mouse anti-Av-β-catenin recognition 28 The expression of Av-β-catenin as an activity indicator of wnt/β-catenin pathway 29 The role of c-Jun varied during A. viride regeneration 30 The inhibitory effect of wnt/β-catenin pathway on cell proliferation may be derived from its anti-apoptotic effect 32 Cell proliferation during regeneration was not activated by wnt/β-catenin pathway 33 References 35 Table 39 Figures: 40 | |
dc.language.iso | en | |
dc.title | Wnt/β-catenin訊息傳遞鏈對Aeolosoma viride前端再生調控之探討 | zh_TW |
dc.title | Wnt/β-catenin Signaling Pathway Regulates Anterior regeneration in Aeolosoma viride | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 李心予(Hsin-yu Lee),李宣書(Hsuan-Shu Lee),管永恕(Yung-Shu Kuan) | |
dc.subject.keyword | 環節動物,再生,wnt 細胞分子傳遞鏈, | zh_TW |
dc.subject.keyword | Annelids,regeneration,wnt signaling pathway, | en |
dc.relation.page | 59 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-08-04 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
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