請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/28098
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 胡哲明(Jer-Ming Hu) | |
dc.contributor.author | Tien-Hao Chang | en |
dc.contributor.author | 張天豪 | zh_TW |
dc.date.accessioned | 2021-06-13T00:01:02Z | - |
dc.date.available | 2007-08-03 | |
dc.date.copyright | 2007-08-03 | |
dc.date.issued | 2007 | |
dc.date.submitted | 2007-07-31 | |
dc.identifier.citation | <b>Albert, V.A., M. H. G. Gustafsson and L. Di Laurenzio.</b> (1998). Ontogenetic systematics, molecular developmental genetics and the angiosperm flower. In Molecular Systematics of Plants Ⅱ, P. Soltis, D., Soltis and J. J. Doyle, ed (New York City, NY, USA: Kluwer Academic Publishers), pp. 349-374.
<b>Alvarez-Buylla, E.R., Pelaz, S., Liljegren, S.J., Gold, S.E., Burgeff, C., Ditta, G.S., de Pouplana, L.R., Martinez-Castilla, L., and Yanofsky, M.F. </b>(2000). An ancestral MADS-box gene duplication occurred before the divergence of plants and animals. Proceedings of the National Academy of Sciences of the United States of America 97, 5328-5333. <b>Baumann, K., Perez-Rodriguez, M., Bradley, D., Venail, J., Bailey, P., Jin, H.L., Koes, R., Roberts, K., and Martin, C.</b> (2007). Control of cell and petal morphogenesis by R2R3 MYB transcription factors. Development 134, 1691-1701. <b>Bremer, B., Bremer, K., Chase, M.W., Reveal, J.L., Soltis, D.E., Soltis, P.S., Stevens, P.F., Anderberg, A.A., Fay, M.F., Goldblatt, P., Judd, W.S., Kallersjo, M., Karehed, J., Kron, K.A., Lundberg, J., Nickrent, D.L., Olmstead, R.G., Oxelman, B., Pires, J.C., Rodman, J.E., Rudall, P.J., Savolainen, V., Sytsma, K.J., van der Bank, M., Wurdack, K., Xiang, J.Q.Y., and Zmarzty, S. </b>(2003). An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG II. Botanical Journal of the Linnean Society 141, 399-436. <b>Castillejo, C., Romera-Branchat, M., and Pelaz, S. </b>(2005). A new role of the Arabidopsis SEPALLATA3 gene revealed by its constitutive expression. Plant Journal 43, 586-596. <b>Chang, S., J. Puryear and J. Cairney. </b>(1993). A simple and efficient method for isolating RNA from pine tree. Plant Molecular Biology Reporter 11, 113-116. <b>Cho, S.C., Jang, S.H., Chae, S.J., Chung, K.M., Moon, Y.H., An, G.H., and Jang, S.K.</b> (1999). Analysis of the C-terminal region of Arabidopsis thaliana APETALA1 as a transcription activation domain. Plant Molecular Biology 40, 419-429. <b>Claβen-bockhoff, R.</b> (1990). Pattern-analysis in pseudanthia. Plant Systematics and Evolution 171, 57-88. Coen, E.S., and Meyerowitz, E.M. (1991). The war of whorls - genetic interactions controlling flower development. Nature 353, 31-37. <b>Colombo, L., Franken, J., Koetje, E., Vanwent, J., Dons, H.J.M., Angenent, G.C., and Vantunen, A.J.</b> (1995). The Petunia MADS Box Gene FBP11 Determines Ovule Identity. Plant Cell 7, 1859-1868. <b>Ditta, G., Pinyopich, A., Robles, P., Pelaz, S., and Yanofsky, M.F.</b> (2004). The SEP4 gene of Arabidopsis thaliana functions in floral organ and meristem identity. Current Biology 14, 1935-1940. <b>Editorial committee of the Flora of Taiwan.</b> (1993). Flora of Taiwan, Volume 3 (Taipei: Editorial committee of the Flora of Taiwan), pp. 35-64. <b>Egea-Cortines, M., Saedler, H., and Sommer, H.</b> (1999). Ternary complex formation between the MADS-box proteins SQUAMOSA, DEFICIENS and GLOBOSA is involved in the control of floral architecture in Antirrhinum majus. Embo Journal 18, 5370-5379. <b>Felsenstein, J.</b> (1985). Phylogenies and the comparative method. American Naturalist 125, 1-15. <b>Force, A., Lynch, M., Pickett, F.B., Amores, A., Yan, Y.L., and Postlethwait, J. </b>(1999). Preservation of duplicate genes by complementary, degenerative mutations. Genetics 151, 1531-1545. <b>Glover, B.J., and Martin, C. </b>(1998). The role of petal cell shape and pigmentation in pollination success in Antirrhinum majus. Heredity 80, 778-784. <b>Glover, B.J., Perez-Rodriguez, M., and Martin, C.</b> (1998). Development of several epidermal cell types can be specified by the same MYB-related plant transcription factor. Development 125, 3497-3508. <b>Honma, T., and Goto, K.</b> (2001). Complexes of MADS-box proteins are sufficient to convert leaves into floral organs. Nature 409, 525-529. <b>Hsieh, W.-P.</b> (2006). Functional analysis of MADS box genes and the investigation of their interactions in regulating floral organ formation from lily (Lilium longiflorum). In Institute of Biotechnology (Taichung City: National Chung Hsing University). <b>Huelsenbeck, J.P., and Ronquist, F.</b> (2001). MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17, 754-755. <b>Hufford, L.</b> (2004). Hydrangeaceae. In The Families and Genera of Vascular Plants, K. Kubitzki, ed (Berlin, Heidelberg, Germany: Springer-Verlag), pp. 202-215. <b>Hufford, L., Moody, M.L., and Soltis, D.E.</b> (2001). A phylogenetic analysis of Hydrangeaceae based on sequences of the plastid gene matK and their combination with rbcl and morphological data. International Journal of Plant Sciences 162, 835-846. <b>Jackson, D., Culianez-Macia, F., Prescott, A. G., Roberts, K. and Martin, C.</b> (1991). Expression patterns of Myb genes from Antirrhinum flowers. Plant Cell 3. <b>Kalivas, A., Pasentsis, K., Polidoros, A.N., and Tsaftaris, A.S. </b>(2007). Heterotopic expression of B-class floral homeotic genes PISTILLATA/GLOBOSA supports a modified model for crocus (Crocus sativus L.) flower formation. DNA Sequence 18, 120-130. <b>Kanno, A., Saeki, H., Kameya, T., Saedler, H., and Theissen, G. </b>(2003). Heterotopic expression of class B floral homeotic genes supports a modified ABC model for tulip (Tulipa gesneriana). Plant Molecular Biology 52, 831-841. <b>Kay, Q.O.N., Daoud, H.S., and Stirton, C.H.</b> (1981). Pigment distribution, light-reflection and cell structure in petals. Botanical Journal of the Linnean Society 83, 57-83. <b>Kramer, E.M., Dorit, R.L., and Irish, V.F.</b> (1998). Molecular evolution of genes controlling petal and stamen development: Duplication and divergence within the APETALA3 and PISTILLATA MADS-box gene lineages. Genetics 149, 765-783. <b>Kramer, E.M., Di Stilio, V.S., and Schluter, P.M.</b> (2003). Complex patterns of gene duplication in the APETALA3 and PISTILLATA lineages of the Ranunculaceae. International Journal of Plant Sciences 164, 1-11. <b>Kranz, H.D., Denekamp, M., Greco, R., Jin, H., Leyva, A., Meissner, R.C., Petroni, K., Urzainqui, A., Bevan, M., Martin, C., Smeekens, S., Tonelli, C., Paz-Ares, J., and Weisshaar, B.</b> (1998). Towards functional characterisation of the members of the R2R3-MYB gene family from Arabidopsis thaliana. Plant Journal 16, 263-276. <b>Lu, C.-Y.</b> (2004). Expression patterns of floral organ identity genes in petaloid structure of Mussaenda spp. (Rubiaceae). In Institute of Ecology and Evolutionary Biology (Taipei City: National Taiwan University). <b>Müller, B.M., Saedler, H., and Zachgo, S.</b> (2001). The MADS-box gene DEFH28 from Antirrhinum is involved in the regulation of floral meristem identity and fruit development. Plant Journal 28, 169-179. <b>Maddison, D.R.a.W.P.M. </b>(2000). MacClade 4: Analysis of phylogeny and character evolution, version 4.0. (Sunderland, Massachusetts, USA: Sinauer Associates). <b>Ng, M., and Yanofsky, M.F.</b> (2000). Three ways to learn the ABCs. Current Opinion in Plant Biology 3, 47-52. <b>Noda, K., Glover, B.J., Linstead, P., and Martin, C.</b> (1994). Flower color intensity depends on specialized cell-shape controlled by a myb-related transcription factor. Nature 369, 661-664. <b>Pelaz, S., Tapia-Lopez, R., Alvarez-Buylla, E.R., and Yanofsky, M.F.</b> (2001a). Conversion of leaves into petals in Arabidopsis. Current Biology 11, 182-184. <b>Pelaz, S., Ditta, G.S., Baumann, E., Wisman, E., and Yanofsky, M.F.</b> (2000). B and C floral organ identity functions require SEPALLATA MADS-box genes. Nature 405, 200-203. <b>Pelaz, S., Gustafson-Brown, C., Kohalmi, S.E., Crosby, W.L., and Yanofsky, M.F.</b> (2001b). APETALA1 and SEPALLATA3 interact to promote flower development. Plant Journal 26, 385-394. <b>Pnueli, L., Abuabeid, M., Zamir, D., Nacken, W., Schwarzsommer, Z., and Lifschitz, E.</b> (1991). The MADS box gene family in tomato - temporal expression during floral development, conserved secondary structures and homology with homeotic genes from Antirrhinum and Arabidopsis. Plant Journal 1, 255-266. <b>Pollock, R., and Treisman, R. </b>(1991). Human SRF-related proteins-DNA-binding properties and potential regulatory targets. Genes & Development 5, 2327-2341. <b>Riechmann, J.L., and Meyerowitz, E.M.</b> (1997). MADS domain proteins in plant development. Biological Chemistry 378, 1079-1101. <b>Riechmann, J.L., Krizek, B.A., and Meyerowitz, E.M.</b> (1996a). Dimerization specificity of Arabidopsis MADS domain homeotic proteins APETALA1, APETALA3, PISTILLATA, and AGAMOUS. Proceedings of the National Academy of Sciences of the United States of America 93, 4793-4798. <b>Riechmann, J.L., Wang, M.Q., and Meyerowitz, E.M.</b> (1996b). DNA-binding properties of Arabidopsis MADS domain homeotic proteins APETALA1, APETALA3, PISTILLATA and AGAMOUS. Nucleic Acids Research 24, 3134-3141. <b>Robles, P., and Pelaz, S.</b> (2005). Flower and fruit development in Arabidopsis thaliana. International Journal of Developmental Biology 49, 633-643. <b>Saitou, N., and Nei, M.</b> (1987). The neighbor-joining method: A new method foe reconstructing phylogenetic trees. Molecular Biology and Evolution 4, 406-425. <b>Schwarz-Sommer, Z., Hue, I., Huijser, P., Flor, P.J., Hansen, R., Tetens, F., Lonnig, W.E., Saedler, H., and Sommer, H.</b> (1992). Characterization of the Antirrhinum floral homeotic MADS-box gene DEFICIENS - evidence for DNA-binding and autoregulation of its persistent expression throughout flower development. Embo Journal 11, 251-263. <b>Stevens, P.F.</b> (2006). Angiosperm Phylogeny Website. Version 7. <b>Stracke, R., Werber, M., and Weisshaar, B.</b> (2001). The R2R3-MYB gene family in Arabidopsis thaliana. Current Opinion in Plant Biology 4, 447-456. <b>Swofford, D.L.</b> (2002). PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods), version 4.0beta10. (Sunderland, Massachusetts, USA: Sinauer Associates). <b>Theissen, G.</b> (2001). Development of floral organ identity: stories from the MADS house. Current Opinion in Plant Biology 4, 75-85. <b>Theissen, G., and Saedler, H.</b> (2001). Plant biology - Floral quartets. Nature 409, 469-471. <b>Theissen, G., Becker, A., Di Rosa, A., Kanno, A., Kim, J.T., Munster, T., Winter, K.U., and Saedler, H.</b> (2000). A short history of MADS-box genes in plants. Plant Molecular Biology 42, 115-149. <b>Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., and Higgins, D.G. </b>(1997). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 4876-4882. <b>Tzeng, T.Y., and Yang, C.H.</b> (2001). A MADS box gene from lily (Lilium longiflorum) is sufficient to generate dominant negative mutation by interacting with PISTILLATA (PI) in Arabidopsis thaliana. Plant and Cell Physiology 42, 1156-1168. <b>Vandenbussche, M., Zethof, J., Royaert, S., Weterings, K., and Gerats, T. </b>(2004). The duplicated B-class heterodimer model: Whorl-specific effects and complex genetic interactions in Petunia hybrida flower development. Plant Cell 16, 741-754. <b>Weigel, and Meyerowitz.</b> (1994). The ABCs of floral homeotic genes. Cell 79, 180-180. <b>Zahn, L.M., Leebens-Mack, J., dePamphilis, C.W., Ma, H., and Theissen, G.</b> (2005). To B or not to B a flower: The role of DEFICIENS and GLOBOSA orthologs in the evolution of the angiosperms. Journal of Heredity 96, 225-240. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/28098 | - |
dc.description.abstract | 大而明顯的花瓣常是植物用來吸引傳粉者的構造,而一些植物會使用整個花序聚集形成一個如同一朵花的假單花(pseudanthium)構造來達到類似的功能。以八仙花科(Hydrangeaceae)植物為例,它的花序最外圍的花常為不孕性並且具有膨大而有顏色的花萼,使整個花序看起來像是一大朵顯眼的花,我們稱這樣膨大而可能扮演花瓣角色的構造為類花瓣(petaloid structure)。本研究採用華八仙(<em>Hydrangea chinensis</em>)作為實驗材料,希望能了解類花瓣的形成機制。
本研究中從華八仙中釣取了花部器官決定的同源基因,包括一個A群基因HycFL、三個B群基因<em>HycPI</em>, <em>HycAP3</em>, <em>HycTM6</em>、一個C群基因<em>HycAG</em>以及一個E群基因<em>HycMADS1</em>,並藉由其保守的C端序列和譜系分析結果進行基因群的確認。利用RT-PCR檢視所釣取基因於花部各部位之表現情形發現,A群基因<em>HycFL</em>,一個B群基因<em>HycTM6</em>,和E群基因<em>HycMADS1</em>在所有的花部器官也均有表現;另外兩個B群基因中,<em>HycAP3</em>表現於花瓣、雄蕊、花柱以及與花萼合生的子房下半部,<em>HycPI</em>則除了在與花萼合生的子房下半部有微弱表現外,僅表現於花瓣以及雄蕊的部分;C群基因<em>HycAG</em>表現於花的內三輪器官但在雄蕊及心皮表現量較高。 以掃描式電子顯微鏡觀察華八仙的花朵,其花的各部位細胞表面均有角質層的加厚,而僅有膨大花萼的近軸面細胞呈現圓錐狀形態。這種圓錐狀細胞通常存於植物的花瓣近軸面細胞,而在金魚草(<em>Antirrhinum majus</em>)的研究中已發現其中的MIXTA基因會調控花瓣近軸面表皮細胞形態,使其呈現圓錐狀突起。因此我們也在華八仙中選殖出了<em>MIXTA</em>的同源基因<em>HycMYB1</em>,RT-PCR的實驗結果顯示此基因會表現於膨大花萼,而在正常的花萼以及花瓣則僅有微弱的表現情形。 依據我們的實驗結果,<em>HycFL</em>、<em>HycTM6</em>、<em>HycMADS1</em>和<em>HycMYB1</em>均在膨大花萼處有表現,顯示這四個基因可能均參與了華八仙中膨大花萼的形成過程。 | zh_TW |
dc.description.abstract | The inflorescence of Hydrangeaceae plants is often aggregated as a pseudanthium, which contains enlarged sepals on peripheral flowers of the inflorescence. The flowers with these petaloid sepals are generally sterile and may play important roles in attracting pollinators. In order to understand the molecular mechanism for the petaloidy formation, floral organ identity genes of <em>Hydrangea chinensis</em> was identified and examined the expression patterns among different organs.
One A-class (<em>HycFL</em>), three B-class (<em>HycPI</em>, <em>HycAP3</em>, <em>HycTM6</em>), one C-class (<em>HycAG</em>) and one E-class (<em>HycMADS1</em>) floral homeotic genes were identified from <em>H. chinensis</em>, and all of the sequences show conserved C-terminal motifs for ABCE class genes, further confirmation for their identities were carried out by separate phylogenetic analyses. RT-PCR results show that the A class homolog <em>HycFL</em>, one B class homolog, <em>HycTM6</em>, and the E-class gene homolog <em>HycMADS1</em> have ubiquitous expression in all floral parts. Another B-class homolog, <em>HycAP3</em>, is expressed in petals, stamens, styles, and lower part of carpels, which is fused to part of normal sepals. In contrast, <em>HycPI</em> is highly expressed in petals and stamens, and weakly in lower part of carpels. The C-class gene homolog, <em>HycAG</em>, is expressed in the inner three whorls of flowers but the expression is higher in stamens and carpels. Under Scanning Electromicroscope examination, the epidermal surfaces of cells on examined floral parts all have cuticular striation, while only adaxial epidermal cells of enlarged sepals show conical shape, a feature commonly found in the epidermal cells of a petal in other flowering plants. Such conical cell formation has been demonstrated to be controlled by a transcription factor, <em>MIXTA</em>, in <em>Antirrhnicum majus</em>. We have also successfully cloned <em>HycMYB1</em>, a homolog of <em>MIXTA</em>. The RT-PCR result shows that <em>HycMYB1</em> is highly expressed in enlarged sepals and much less in normal sepals and petals. Since HycFL, <em>HycTM6</em>, <em>HycMADS1</em> and <em>HycMYB1</em> are all expressed in enlarged sepals, they are the candidate genes involved in the formation of petaloid sepals of <em>Hydrangea chinensis</em>. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T00:01:02Z (GMT). No. of bitstreams: 1 ntu-96-R94b44008-1.pdf: 9067314 bytes, checksum: 1381d8478468479e3ec1c72a3b695d2a (MD5) Previous issue date: 2007 | en |
dc.description.tableofcontents | 中文摘要..................................................1
英文摘要..................................................2 壹、實驗背景介紹..........................................4 一、花部形態與花被的演化..................................4 1. 花部形態...............................................4 2. 花被的演化.............................................4 3. 假單花.................................................5 4. 假單花於山茱萸目(Cornales)中的演化.....................5 二、花部器官發育的分子調控機制............................6 1. ABCDE model............................................6 2. MADS-box基因與Floral quartet model.....................7 3. 花瓣表皮細胞與MIXTA基因................................8 4. 類花瓣與ABCDE model....................................9 三、實驗假設與目的.......................................11 貳、材料與方法...........................................15 一、本實驗使用之八仙花科植物介紹.........................15 二、台灣產八仙花科植物樣本之收集.........................15 三、掃瞄式電子顯微鏡(SEM)樣本之製備與操作................18 四、台灣產八仙花科植物花部器官決定同源基因之選殖.........18 五、基因之譜系分析.......................................23 六、植物DNA的萃取........................................24 七、南方轉漬分析 (Southern blot analysis)................26 八、花部同源基因表現分析.................................28 參、結果.................................................44 一、華八仙花部形態觀察...................................44 二、華八仙花部同源基因以及MYB家族基因之選殖與南方轉漬分析法之檢測...................................................44 三、譜系分析.............................................47 四、以RT-PCR檢測華八仙花部同源基因與MYB家族基因於各花部器官之表現...................................................48 肆、討論.................................................76 一、華八仙花部表現細胞形態與傳粉機制.....................76 二、華八仙花部同源基因於不同花部之表現...................76 三、南方轉漬分析結果.....................................78 四、華八仙MYB家族同源基因之譜系分析......................78 五、膨大花萼之形成與候選基因.............................79 伍、結論.................................................82 陸、參考資料.............................................84 柒、附錄.................................................94 | |
dc.language.iso | zh-TW | |
dc.title | 華八仙(八仙花科)類花瓣構造與花部器官決定基因之研究 | zh_TW |
dc.title | Petaloidy and floral homeotic genes in <em>Hydrangea chinensis</em> (Hydrangeaceae) | en |
dc.type | Thesis | |
dc.date.schoolyear | 95-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 王俊能(Chun-Neng Wang),邱少婷(Shau-Ting Chiu),楊長賢(Chang-Hsien Yang),陳虹樺(Hong-Hwa Chen) | |
dc.subject.keyword | MADS-box基因,華八仙,MIXTA,類,花瓣, | zh_TW |
dc.subject.keyword | MADS-box genes,Hydrangea,MIXTA,petaloid sturcture, | en |
dc.relation.page | 119 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2007-07-31 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生態學與演化生物學研究所 | zh_TW |
顯示於系所單位: | 生態學與演化生物學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-96-1.pdf 目前未授權公開取用 | 8.85 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。