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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 連雙喜 | |
| dc.contributor.author | Yi-Cheng Lin | en |
| dc.contributor.author | 林奕丞 | zh_TW |
| dc.date.accessioned | 2021-06-08T04:47:22Z | - |
| dc.date.copyright | 2009-08-03 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-07-30 | |
| dc.identifier.citation | 參考文獻
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23205 | - |
| dc.description.abstract | 沃斯田鐵不銹鋼具有優良的性質，故常被作為骨科植入之用，但由於成分含鎳有毒性之慮，加上鎳價上漲使得煉鋼原料成本大漲，為了降低鎳的含量而可使用氮取代鎳元素。台大實驗室曾運用電漿熔煉探討研製高氮無鎳不銹鋼與製程參數之關係，利用錳來提高氮在沃斯田鐵裡的溶解度並求得最佳之合金成分及熔煉參數15Cr-22Mn-3Mo，因此本實驗以15Cr-22Mn-3Mo三種加錳無鎳的不同氮含量之高氮不銹鋼，並搭配市售316L不銹鋼作機械腐蝕性質上的比較加氮和錳的影響，然後也就針對細胞毒性反應、發炎反應和細胞黏附等實驗選擇Raw264.7巨噬細胞來進行評估高氮無鎳不銹鋼生物相容性。實驗結果證明材料硬度因含氮量的增加而提高，並且經由動態腐蝕電位測試儀配合模擬人體中的環境下使用細胞培養液，也可看出材料因氮含量提升而抗腐蝕能力提高，孔蝕電位也增加；材料與老鼠巨噬細胞Raw264.7培養模擬體外與細胞接觸的情形發現，細胞毒性測試顯示熔煉的三個合金其細胞存活率和商用316L相比並沒有顯著差異，發炎反應試驗材料不會引起巨噬細胞產生一氧化氮，但是熔煉的三個合金卻有引發ROS之慮；細胞黏附實驗方面，顯示合金粗糙表面有助於巨噬細胞黏附生長穩定。由實驗結果可看出藉由添加氮氣來強化材料其抗腐蝕性質的能力與錳和氮氣的綜合作用有關，耐腐蝕性質的提升有利於增加材料植入的生物相容性。 | zh_TW |
| dc.description.abstract | Due to the good properties of austenitic stainless steel, it is used for implanted orthopedic materials. But as result of the nickel'toxic potential and the nickel price rise causes the cost of steel-making rise sharply, we can replace the nickel content for nitrogen, and the manganese may enhance the solubility of nitrogen content in austenitic steel. Our laboratory once used the plasma melting to develop the relations between high nitrogen nickel-free stainless steel and melting parameter, and obtain the best alloying constituent and smelting parameter 15Cr-22Mn-3Mo.
Therefore this research will aim at the different nitrogen contents of self-made ingot to discuss the machanical corrosion properties, and study on the alloy' biocompatibility with mouse macrophage RAW264.7. This experiment melt the 15Cr-22Mn-3Mo in ratio by plasma melting, , and take three ingot to proceed the dynamic electric potential polarization curve test, the cytotoxicity test, the cell inflammation test, as well as the cell adhesion ability to judge the material'availability degree. Experimental results support increase of hardness because of the increase of nitrogen content, and using the cell nutrient fluid by way of the dynamic corrosion potential test, also show that corrosion resistance ability enhance and pitting corrosion resistance do also. However, simulating the situation of material contacting the mouse macrophage raw264.7 demonstrate the three ingots survival percentages and commercial 316L compare do not make the big difference, and material do not cause the macrophage to produce the nitrogen monoxide, but have the potential to induce macrophage producing ROS. In cell adhesion test, demonstrate that the alloy's rough surface is helpful in the macrophage adhesion and be stable to growth. In brief, alloying Mn may degrade the resistance of the HNSSs to localized corrosion. The improvement of corrosion resistance caused by the addition of nitrogen could be attributed to the synergistic effects of nitrogen and manganese in the HNSSs. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T04:47:22Z (GMT). No. of bitstreams: 1 ntu-98-R96527055-1.pdf: 5835002 bytes, checksum: 1dbc5a613c9fe398b15c6e22afa632b8 (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 目錄
口試委員會審定書.....................................................................................I 誌謝...........................................................................................................II 中文摘要..................................................................................................XI 英文摘要.................................................................................................XII 縮寫對照表...........................................................................................XIV 第一章 介紹..............................................................................................1 1. 生醫材料.............................................................................................1 1.1常用骨科生醫合金.............................................................................1 1.2 不銹鋼.............................................................................................6 1.2.1 高氮不銹鋼................................................................................6 1.2.1.1不銹鋼的含鎳問題................................................................6 1.2.1.2 取代含鎳的不銹鋼：氮.......................................................8 1.2.1.3 引入高氮含量的方法...........................................................8 1.2.1.4 添加合金元素對不銹鋼性質之影響.................................10 1.2.2 不銹鋼的孔蝕問題..................................................................12 1.2.2.1孔蝕理論..............................................................................12 1.2.2.2 電化學方法測定孔蝕電位.................................................12 2. 金屬合金植入體內...........................................................................17 2.1材料腐蝕反應引發的問題............................................................17 2.2評估材料生物相容性....................................................................18 3. 研究動機...........................................................................................21 第二章 實驗與方法................................................................................23 1. 電漿熔煉步驟...................................................................................23 2.試片準備和處理.................................................................................23 2.1 切割試片.......................................................................................23 2.2 試片表面粗糙度.............................................................................24 3. 材料性質檢驗...................................................................................24 3.1 金相顯微鏡觀察...........................................................................24 3.2 電子顯微鏡和EDX成分析 .........................................................24 3.3 ICP-AES成分分析.........................................................................24 3.4 氧氮分析儀（Nitrogen/Oxygen determinator）..............................24 3.5 X-ray繞射分析...............................................................................25 3.6 微硬度（Hardness of Vicker；簡稱HV）.....................................25 3.7 動態電位極化掃描分析...............................................................25 4.材料進行生物實驗分析.....................................................................25 4.1 細胞培養.......................................................................................26 4.2 細胞存活率...................................................................................26 4.3 Cytospin和Giemsa染色看細胞貼附合金上的型態....................26 4.4 TUNEL Assay偵測細胞凋亡........................................................26 4.5偵測一氧化氮（NO）.....................................................................27 4.6 偵測活性氧（ROS）.......................................................................27 4.7 黏附試驗.......................................................................................28 4.8 統計分析.......................................................................................28 第三章 結果與討論................................................................................35 1. 合金熔煉成分：15Cr-22Mn-3Mo...................................................35 2. 高氮無鎳不銹鋼的型態與微結構之觀察.......................................37 3. XRD繞射分析合金是否為沃斯田鐵結構（FCC）........................44 4. 加氮合金材料之硬度量測...............................................................51 5. 加氮合金的耐腐蝕性能力測試.......................................................53 6. 合金對於巨噬細胞raw 264.7之細胞毒性檢測.............................57 7. 合金是否引發巨噬細胞raw 264.7釋放發炎物質一氧化氮.........59 8. 合金是否引發巨噬細胞raw 264.7釋放發炎物質活性氧.............61 9. 細胞黏附試驗...................................................................................66 10. 細胞的發炎物質是否會影響材料壽命...........................................69 第四章 結論............................................................................................75 參考文獻..................................................................................................76 附錄..........................................................................................................80 | |
| dc.language.iso | zh-TW | |
| dc.subject | 無鎳 | zh_TW |
| dc.subject | 不銹鋼 | zh_TW |
| dc.subject | 生物相容性 | zh_TW |
| dc.subject | 耐腐蝕性 | zh_TW |
| dc.subject | 高氮 | zh_TW |
| dc.subject | biocompatibility | en |
| dc.subject | stainless steel | en |
| dc.subject | Ni-free | en |
| dc.subject | high nitrogen | en |
| dc.subject | corrosion resistance | en |
| dc.title | 高氮無鎳不銹鋼之機械性質與生物相容性研究 | zh_TW |
| dc.title | Study on Mechanical Properties and Biocompatibility of High Nitrogen Nickle-free stainless steel | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李新楊,楊台鴻 | |
| dc.subject.keyword | 高氮,無鎳,不銹鋼,耐腐蝕性,生物相容性, | zh_TW |
| dc.subject.keyword | high nitrogen,Ni-free,stainless steel,corrosion resistance,biocompatibility, | en |
| dc.relation.page | 80 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2009-07-30 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 材料科學與工程學研究所 | zh_TW |
| 顯示於系所單位： | 材料科學與工程學系 | |
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