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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 江文章(Wenchang Chiang) | |
dc.contributor.author | Sz-Chi Chen | en |
dc.contributor.author | 陳思綺 | zh_TW |
dc.date.accessioned | 2021-06-08T04:19:14Z | - |
dc.date.copyright | 2011-08-26 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-18 | |
dc.identifier.citation | 中山宗春。1960。薏苡仁抗癌作用之研究。日本外科學會誌。61: 234。
王思涓。2002。薏苡籽實中特殊生理機能性成分的定量分析與比較。國立臺灣大學食品科技研究所碩士論文。台北。 江政哲。2001。以固相萃取法及氣相層析質譜儀對水環境中動情激素類有機污染物之分析與研究。國立中央大學化學研究所碩士論文。桃園。 吳文慈。2005。蒟蒻補充劑對人體大腸癌發生相關因子之調節作用。中山醫學大學營養研究所碩士論文。台中。 沈明來。2007。試驗設計學,第三版修訂版。p557-589。九州圖書文物有限公司。 李明怡。2008。薏仁麩皮中防癌及抗發炎活性成分之分離與鑑定。國立臺灣大學食品科技研究所博士論文。台北。 吳珊珊。2000。茶多酚之萃取及其除口臭效果之研究。國立臺灣大學食品科技研究所碩士論文。台北。 金安兒和郁凱衡。1993。冷凍乾燥胡蘿蔔之最適操作條件。食品科學 。20:21-32。 林紓慧。2007。薏苡麩皮乙醇萃取物及乙醇萃取殘餘物對F344鼠大腸癌前期病變預防效果之探討。臺北醫學大學保健營養學研究所碩士論文。台北。 林嘉瑜。2002。反應曲面法之改良研究-權重平滑搜尋方向與實驗區域改變規則之探討。國立成功大學工業管理研究所碩士論文。台南。 林穎群。2010。擠壓加工對於產品中番茄紅素組成及生理活性的影響。國立臺灣大學食品科技研究所碩士論文。台北。 范振家。1988。米粉中添加甘藷澱粉所製得擠壓產品理化性質之探討。國立臺灣大學食品科技研究所碩士論文。台北。 洪哲穎和陳國誠。1992。回應曲面實驗設計法在微生物酵素生產之應用。化工期刊。39:3-18。 夏詩閔。2001。薏苡籽實萃取物對大鼠卵巢性類固醇激素、腦下腺黃體促素和濾泡刺激素分泌之效應。國立臺灣大學食品科技研究所碩士論文。台北。 夏詩閔。2006。薏苡麩皮及薏苡殼甲醇萃取物對大鼠卵巢顆粒細胞分泌孕酮之效果。國立臺灣大學食品科技研究所博士論文。台北。 陳心瀅。2005。薏苡殼中酚類化合物與薏苡麩皮甲醇萃取物中乙酸乙酯和正丁醇次區分層對抑制 Tyrosinase活性與消除自由基作用之探討。國立海洋大學食 品科學系碩士論文。基隆。 陳宏彰。2010。薏仁麩皮活性成分之分離與鑑定及其對過敏免疫反應之調節研究。國立臺灣大學食品科技研究所博士論文。台北。 許倖華。2008。薏仁麩皮乙醇萃取物之乙酸乙酯區分物對DMH誘發F344鼠大腸癌前病變之預防效果。國立臺灣大學食品科技研究所碩士論文。台北。 黃士禮和江文章。1999。薏苡杍實各部位之組成及其丙酮萃取液之抗突變作用。食品科學。261:21-130. 奧村彩。2010。韃靼蕎麥殼 (Fagopyrum tartaricum Gaerth) 的功能性成分萃取。國立中興大學食品暨應用生物科技學系所碩士論文。台中。 劉家余。2010。薏仁麩皮萃取物乙酸乙酯區分層之抗發炎效果。國立臺灣大學食品科技研究所碩士論文。台北。 蔡永生和張如華。1986。茶葉可溶分萃取之研究。台灣省茶業研究彙報。 鄭妃彣。2006。薏苡麩皮甲醇萃取物之細胞毒性成分分離與純化。國立臺灣大學食品科技研究所碩士論文。台北。 賴進此。2011。新穎性萃取技術於食品開發之應用。食品工業。4:1-4. 鍾尹如和丁望賢。2005。固態基質中多溴聯苯醚萃取方法之新發展。JCCS。63:343-352. 闞建全。2007。食品化學,第二版。p139-139。新文京開發出版股份有限公司。 Aldoori, W. H.; Giovannucci, E. L.; Rimm, E. B. A prospective study of diet and the risk of symptomatic diverticular disease in men. Am. J. Clin. Nutr. 1994, 60, 757-764. Aoki M.; Tuzihara N. Effects of the hatomugi (Coix lachryma-jobi L. var. ma-yuen) on the hyperlipidemia in rats. 日本家政學会誌 1985, 36, 27-33. Arranz, S.; Saura-Calixto, F.; Shaha, S.; Kroon, P. A. High contents of nonextractable polyphenols in fruits suggest that polyphenol contents of plant foods have been underestimated. J. Agric. Food Chem. 2009, 57, 7298-7803. Bachmeier, B.; Nerlich, A. G.; Iancu, C. M.; Cilli, M.; Schleicher, E.; Vene, R.; Dell'Eva, R.; Jochum, M.; Albini, A.; Pfeffer, U. The chemopreventive polyphenol Curcumin prevents hematogenous breast cancer metastases in immunodeficient mice. Cell Physiol. Biochem. 2007, 19, 137-152. Bazykina, N. I.; Nikolaevskii, A. N.; Filippenko, T. A.; Kaloerova, V. G. Optimization of condition for the extraction of natural antioxidants from raw plant materials. Pharm. Chem. J. 2002, 36(2), 100-103. Bijkerk, C. J.; de Wit, N. J.; Muris, J. W. M. Soluble or insoluble fibre in irritable bowel syndrome in primary care? Randomised placebo controlled trial. BMJ. 2009, 339, b3154. Bors, W.; Michel, C. Chemistry of the antioxidant effect of polyphenols. Ann. N. Y. Acad. Sci. 2002, 957, 57-69. Cacace, J. E.; Mazza, G. Optimization of extraction of anthocyanins from black currants with aqueous ethanol. J. Food Chem. 2003, 68(1), 240-248. Chandrika, L. P.; Fereidoon, S. Optimization of extraction of phenolic compounds from wheat using response surface methodology. Food chem. 2005, 93, 47-56. Chang C. C., Yang M. H., Wen H. M. and Chern J. C. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Ana. 2002, 10, 178-182. Chen, P. N.; Kuo, W. H.; Chiang, C. L.; Chiou, H. L.; Hsieh, Y. S.; Chu, S. C. Black rice anthocyanins inhibit cancer cells invasion via repressions of MMPs and u-PA expression. Chem. Biol. Interact. 2006, 163, 218-229. Chew, K. K.; Na, S. Y.; Thoo, Y. Y.; Khoo, M. Z.; Wan Aida, W. M.; Ho, C. W. Effect of ethanol concentration, extraction time and extraction temperature on the recovery of phenolic compounds and antioxidant capacity of Centella asiatica extracts. International Food Research Journal 2011, 18, 566-573. Chung, C. P.; Hsu, H. Y.; Huang, D. W.; Hsu, H. H.; Lin, J. T.; Shih, C. K.; Chiang, W. Ethyl acetate fraction of adlay bran ethanolic extract inhibits oncogene expression and suppresses DMH-induced preneoplastic lesions of the colon in F344 rats through an anti-inflammatory pathway. J. Agric. Food Chem. 2010, 58, 7616-7623. Cotton, F. A.; Wilkinson, G. Advanced Inorganic Chemistrys – A Comprehensive Text. Wiley: New York, USA, 1980. Dietmar, K.; Achim, C.; Reinhold, C.; Andreas, S. Polyphenol screening of pomace from red and white grape varieries (Vitis vinifera L.) by HPLC-DAD-MS/MS. J. Agric. Food Chem. 2004, 52, 4360-4367. Du, H.; van der, A. D. L.; Boshuizen, H. C. Dietary fiber and subsequent changes in body weight and waist circumference in European men and women. Am. J. Clin. Nutr. 2010, 91, 329 - 336. Dziedzic, S. Z.; Hudson, B. J. F. Polyhydroxy chalcones and flavanones as antioxidants for edible oils. Food Chem. 1983, 12, 205-212. Farmer, E. H.; Bloomfield, G. F.; Sundralingam A.; Sutton, D. A. The course and mechanism of autoxidation reactions in olefinic and polyolefinic substances, including rubber. Trans Faraday Soc.1942, 38, 348-356. Frankel, E. N. Lipid oxidation: mechanisms, products and biological significance. J. Am. Oil Chem. Soc. 1984, 61-12, 1908-1916. Galati, G.; Teng, S.; Moridani, M. Y.; Chan, T. S.; O'Brien, P. J. Cancer chemoprevention and apoptosis mechanisms induced by dietary polyphenolics. Drug Metabol. Drug Interact. 2000, 17, 311-349. Hodek, P.; Trefil, P.; Stiborova, M. Flavonoids-potent and versatile biologically active compounds interacting with cytochromes P450. Chem. Biol. Interact. 2002, 139, 1-21. Hong, J.; Smith, T. J.; Ho, C. T.; August, D. A.; Yang, C. S. Effects of purified green and black tea polyphenols on cyclooxygenase- and lipoxygenase-dependent metabolism of arachidonic acid in human colon mucosa and colon tumor tissues. Biochem. Pharmacol. 2001, 62, 1175-1183. Hou, D. X.; Yanagita, T.; Uto, T.; Masuzaki, S.; Fujii, M. Anthocyanidins inhibit cyclooxygenase-2 expression in LPS-evoked macrophages: structure-activity relationship and molecular mechanisms involved. Biochem. Pharmacol. 2005, 70, 417- 425. Hou, D. X.; Masuzaki, S.; Hashimoto, F.; Uto, T.; Tanigawa, S.; Fujii, M.; Sakata, Y. Green tea proanthocyanidins inhibit cyclooxygenase-2 expression in LPS-activated mouse macrophages: molecular mechanisms and structure-activity relationship. Arch. Biochem. Biophys. 2007, 460, 67-74. Huang, D. W.; Chung, C. P.; Kuo, Y. H.; Lin, Y. L.; Chiang, W. Identification of compounds in adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) seed hull extracts that inhibit lipopolysaccharide-induced inflammation in RAW 264.7 macrophages. J. Agric. Food Chem. 2009, 57, 10651-10657. Huss, H. H. Quality and quality changes in fresh fish. Food and Agriculture Organization of the United Nations. Rome. 1995. Imanishi, H.; Sasaki, Y. F.; Ohta, T.; Watanabe, M.; Kato, T.; Shirasu, Y. Tea tannin components modify the induction of sister-chromatid exchanges and chromosome aberrations in mutagentreated cultured mammalian cells and mice. Mutat. Res. 1991, 259, 79-87. Jacobs, E. T.; Lanza, E.; Alberts, D. S. Fiber, sex, and colorectal adenoma: results of a pooled analysis. Am. J. Clin. Nutr. 2006, 83, 343-349. Jin, D.; Russell, J. M. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules 2010, 15, 7313-7352. Kampa, M.; Nifli, A. P.; Notas, G.; Castanas, E.Polyphenols and cancer cell growth. Rev. Physiol. Biochem. Pharmacol. 2007, 159, 79-113. Kanner, J.; German, J. B.; Kinsella, J. E. Initiation of lipid peroxidation in biological systems. Crit. Rev. Food Sci. Nutr. 1987, 25-4, 317-363. Karlsen, A.; Retterstol, L.; Laake, P.; Paur, I.; Kjolsrud-Bohn, S.; Sandvik, L.; Blomhoff, R. Anthocyanins inhibit nuclear factor-kappaB activation in monocytes and reduce plasma concentrations of pro-inflammatory mediators in healthy adults. J. Nutr. 2007, 137, 1951-1954. Labarbe, B.; Cheynier, V.; Brossaud, F.; Souquet, J. M.; Moutounet, M. Quantitative fractionation of grape proanthocyanidins according to their degree of polymerization. J. Agric. Food Chem. 1999, 47, 2719-2723. Lala, G.; Malik, M.; Zhao, C.; He, J.; Kwon, Y.; Giusti, M. M.; Magnuson, B. A. Anthocyaninrich extracts inhibit multiple biomarkers of colon cancer in rats. Nutr. Cancer 2006, 54, 84-93. Leite, A.; de Freitas, F. A.; Yunes, J. A.; Arruda, P. Nucleotide sequence of a cDNA clone encoding γ-coixin from Coix lacryma-jobi seeds. Plant Physiol. 1991, 97, 1604-1605. Liu, R. H. Potential synergy of phytochemicals in cancer prevention: mechanism of action. J. Nutr. 2004, 134, 3479-3485. Marian, N.; Fereidoon, S. Extraction and analysis of phenolics in food. J. Chromatogr. A 2004, 1054, 95-111. Metivier, R. P.; Francis, F. J.; Clydesdale, F. M. Solvent extraction of anthocyanins from wine pomace. J. Food Sci. 1980, 45, 1099-1100. Meyer, A. S. Enzymatic upgrading of antioxidant phenolics in berry juices and press residues. Fruit Process. 2005, 15, 382-387. Mistry, B. S. and Min, D. B. Oxidized flavor compounds inedible oils. In “Off-Flavors in Foods and Beverages.” Edited by Charalambous, G. pp.171-209. Elsevier, Amsterdam. 1992. Mojzis, J.; Varinska, L.; Mojzisova, G.; Kostova, I.; Mirossay, L. Antiangiogenic effects of flavonoids and chalcones. Pharmacol. Res. 2008, 57, 259-265. Nathan, R. P.; Julia L. B. A review of the antioxidant mechanisms of polyphenol compounds related to iron binding. Cell Biochem. Biophys. 2009. 53, 75-100. Nothlings, U.; Schulze, M. B.; Weikert, C. Intake of vegetables, legumes, and fruit, and risk of all-cause, cardiovascular, and cancer mortality in a European diabetic population. J. Nutr. 2008, 138, 775-781. Gacula, M. H.; Aquilera, J. M. A physicochemical model for extrusion of corn starch. J. Food Sci. 1984, 49, 40-43. Gauliard, B.; Grieve, D.; Wilson, R.; Crozier, A.; Jenkins, C.; Mullen, W. D.; Lean, M. The effects of dietary phenolic compounds on cytokine and antioxidant production by A549 cells. J. Med. Food 2008, 11, 382-384. Gropper, S. S.; Smith, J. L.; Groff, J. L. Advanced nutrition and human metabolism. 2009. Fifth edition. p119. Guyot, S.; Marnet, N.; Drilleau, J. Thiolysis-HPLC characterization of apple procyanidins covering a large range of polymerization states. J. Agric. Food Chem. 2001, 49, 14-20. Ogasawara, M.; Matsunaga, T.; Suzuki, H. Differential effects of antioxidants on the in vitro invasion, growth and lung metastasis of murine colon cancer cells. Biol. Pharm. Bull. 2007, 30, 200-204. Park, N. Y.; Lee, G. D.; Jeong, Y. J.; Kwon, J. H. Optimization of extraction conditions for physicochemical properties of ethanol eatracts from Chrysanthemum boreale. J. Korean Society for Food Sci. and Nutr. 1998, 27, 585-590. Pergola, C.; Rossi, A.; Dugo, P.; Cuzzocrea, S.; Sautebin, L. Inhibition of nitric oxide biosynthesis by anthocyanin fraction of blackberry extract. Nitric Oxide 2006, 15, 30-39. Perron, N. R.; Brumaghim, J. L. A review of the antioxidant mechanisms of polyphenol compounds related to iron binding. Cell Biochem. Biophys. 2009, 53, 75-100. Pinelo, M.; Del Fabbro, P.; Marzocco, L.; Nunez, M. J.; Vicoli, M. C. Optimization of continuous phenol extraction from Vitisvinifera byproducts. Food Chem, 2005, 92, 109-117. Prior, R. L.; Lazarus, S. A.; Cao, G.; Muccitelli, H.; Hammerstone, J. F. Identification of procyanidins and anthocyanins in blueberries and cranberries (Vaccinium spp.) using highperformance liquid chromatography/mass spectrometry. J. Agric. Food Chem. 2001, 49, 1270-1276. Rice-Evans, C. A.; Miller, N. J.; Paganga, G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic. Biol. Med. 1996, 20, 933-956. Rimm, E. B.; Ascherio, A.; Giovannucci, E. Vegetable, fruit, and cereal fiber intake and risk of coronary heart disease amongmen. JAMA. 1996, 275, 447-451. Rodtjer, A.; Skibsted, L. H.; Andersen, M. L. Antioxidative and prooxidative effect of extracts made from cherry liqueur pomace. Food Chem. 2006, 99, 6-14. Rosental, I. Photooxidation of foods. In“singlet oxygen.”Edited by Frimer, A. A. 1985, Vol. 4, pp.145. CRC Press, Boca Raton, Fla. Seeram, N. P.; Adams, L. S.; Zhang, Y.; Lee, R.; Sand, D.; Scheuller, H. S.; Heber, D. Blackberry, black raspberry, blueberry, cranberry, red raspberry, and strawberry extracts inhibit growth and stimulate apoptosis of human cancer cells in vitro. J. Agric. Food Chem. 2006, 54, 9329-9339. Shahidi, F.; Wanasundara, P. K. Phenolic antioxidants. Crit. Rev. Food Sci. Nutr. 1992, 32, 67-103. Shi, J.; Nawaz, H.; Pohorly, J.; Mittal, G.; Kakuda, Y.; Jiang, Y. Extraction of polyphenolics from plant material for functional foods-engineering and technology. Food Rev. Int. 2005, 21, 139-166. Singleton, V. L.; Orthofer, R.; Lamuela-Raventós, R. M. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Meth. Enzymol. 1999, 299, 152-178. Slavin, J. L. Dietary fiber and body weight. Nutr. 2005, 21, 411-418. Slivova, V.; Zaloga, G.; DeMichele, S. J.; Mukerji, P.; Huang, Y. S.; Siddiqui, R.; Harvey, K.; Valachovicova, T.; Sliva, D. Green tea polyphenols modulate secretion of urokinase plasminogen activator (uPA) and inhibit invasive behavior of breast cancer cells. Nutr. Cancer 2005, 52, 66-73. Spormann, T. M.; Albert, F. W.; Rath, T.; Dietrich, H.; Will, F.; Stockis, J. P.; Eisenbrand, G.; Janzowski, C. Anthocyanin/polyphenolic-rich fruit juice reduces oxidative cell damage in an intervention study with patients on hemodialysis. Cancer Epidemiol. Biomarkers Prev. 2008, 17, 3372-3380. Stacey, J. B. A review of dietary fiber and health: focus on raisins. J. Med. Food. 2011. (Article in press) Suzuki, R.; Rylander-Rudqvist, T.; Ye, W. Dietary fiber intake and risk of postmenopausal breast cancer defined by estrogen and progesterone receptor status. A prospective study among Swedish women. Int. J. Cancer.2008, 122, 403 -412. Tanimura, S.; Kadomoto, R.; Tanaka, T.; Zhang, Y. J.; Kouno, I.; Kohno, M. Suppression of tumor cell invasiveness by hydrolyzable tannins (plant polyphenols) via the inhibition of matrix metalloproteinase-2/-9 activity. Biochem. Biophys. Res. Commun. 2005, 330, 1306-1313. Tsoyi, K.; Park, H. B.; Kim, Y. M.; Chung, J. I.; Shin, S. C.; Lee, W. S.; Seo, H. G.; Lee, J. H.; Chang, K. C.; Kim, H. J. Anthocyanins from black soybean seed coats inhibit UVB-induced inflammatory cylooxygenase-2 gene expression and PGE2 production through regulation of the nuclear factor-kappaB and phosphatidylinositol 3-kinase/Akt pathway. J. Agric. Food Chem. 2008, 56, 8969-8974. Vayalil, P. K.; Mittal, A.; Katiyar, S. K.Proanthocyanidins from grape seeds inhibit expression of matrix metalloproteinases in human prostate carcinoma cells, which is associated with the inhibition of activation of MAPK and NF kappa B. Carcinogenesis 2004, 25, 987-995. Webster, R. P.; Gawde, M. D.; Bhattacharya, R. K. Protective effect of rutin, a flavonol glycoside, on the carcinogen-induced DNA damage and repair enzymes in rats. Cancer Lett. 1996, 109, 185-191. Weickert, M. O.; Pfeiffer, A. F. H; Metabolic effects of dietary fiber consumption and prevention of diabetes. J. Nutr. 2008, 138, 439-442. White, B. L.; Howard, L. R.; Prior, R. L. Release of bound procyanidins from cranberry pomace byalkaline hydrolysis. J. Agric. Food Chem. 2010, 58, 7572-7579. Xu, B. J.; Chang, S. K.; A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J. Food Sci. 2007, 72, S159-166. Yilmaz, Y.; Toledo, R. T. Oxygen radical absorbance capacities of grape/wine industry byproducts and effect of solvent type on extraction of grape seed polyphenols. J. Food Compost. Anal. 2006, 19, 41-44. Yoshino, M.; Murakami, K. Interaction of iron with polyphenolic compounds: application to antioxidant characterization. Anal. Biochem. 1998, 257, 40-44. Zhang, Y.; Seeram, N. P.; Lee, R.; Feng, L.; Heber, D. Isolation and identification of strawberry phenolics with antioxidant and human cancer cell antiproliferative properties. J. Agric. Food Chem. 2008, 56, 670-675. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/22498 | - |
dc.description.abstract | 薏仁 (Coix lachryma-jobi L.var. ma-yuen Stapf.),為禾本科一年生草本作物,自古即被認為是藥食兼用的材料,能抑制腫瘤細胞生長、抗發炎。薏仁麩皮 (adlay bran, AB)占糙薏仁的7%,是加工過程重要的副產物。AB富含蛋白質、脂質、膳食纖維,和一些具有生物活性的化合物,如酚類化合物和植物固醇。然而,具有多種價值的AB卻當成廢棄物處理,不曾善加利用。因此本研究目的欲尋找萃取AB酚類化合物的最適條件。
利用反應曲面法 (response surface methodology, RSM),探討總萃取率、總酚含量及總類黃酮含量來評估萃取 AB 酚類化合物的最適條件。根據預實驗的結果,萃取溫度和乙醇濃度為影響萃取機能性成分的重要因素,並利用旋轉式中心組合設計 (rotatable central composite design, RCCD) 訂出13組萃取條件,其中五組條件為中心點,接著使用二階模式探討不同萃取條件下對萃取率、總酚含量、總類黃酮含量的影響。實驗結果發現,薏仁麩皮乙醇萃取物1 (adlay bran ethanolic extract 1, ABE1) 的萃取率、總酚含量、總類黃酮含量都能符合所建立的迴歸模式,且能解釋的變異程度超過78%。綜合萃取結果,最適的萃取條件為55%乙醇、50℃的萃取溫度。經高效能液相層析儀 (high-performance liquid chromatography, HPLC) 定量得知,薏仁麩皮乙醇萃取物1的乙酸乙酯區分層 (ethyl acetate fraction of adlay bran ethanolic extract 1, ABE1-Ea) 的酚類化合物含量依序為對羥基苯甲酸 (p-hydroxybenzoic acid, 114.9 μg/g sample)、香草醛 (vanillin, 63.7 μg/g sample) 和阿魏酸 (ferulic acid, 61.6 μg/g sample)。本研究結果顯示,薏仁麩皮經萃取有助於提升機能性及加工利用性,具有開發成保健素材的潛力。 | zh_TW |
dc.description.abstract | Adlay ( Coix lachryma-jobi L. var. ma-yuen Stapf ) is an one year grass crop that has long been used as a traditional Chinese medicine (TCM) to treat tumor and inflammation. In addition, adlay bran (AB) is an important byproduct of the milling process, representing 7% of the dehulled adlay. AB is rich in proteins, lipids, dietary fibers and bioactive compounds, such as phenolic compounds and phytosterols. However, AB which contains considerable amounts of valuable compounds is dealt as agricultural waste without properly utilization. The aim of this study is to search optimization extraction conditions of phenolic compounds from AB.
The extraction of phenolic compounds from AB was optimized by the simultaneous maximization of the yield in adlay bran extract, total phenolics (TP), total flavanoids (TFA), using the response surface methodology (RSM). According to the preliminary results, extraction temperature and ethanol concentration were selected as the most affective factors for extracting. A rotatable central composite design (RCCD) consisting of 13 experimental runs with five replicates at the center point was then applied and a second-order polynomial model was used to describe the experimental data regarding the yield in adlay bran extract 1 (ABE1), TP and TFA. The experimental results fitted well to the model and more than 78% of the variability was explained. The optimized conditions were 55% ethanol and 50℃. Based on the high-performance liquid chromatography (HPLC) analysis, the more abundant phenolic compounds in ethyl acetate fraction of adlay bran ethanolic extract 1 (ABE1-Ea) in orders are p-hydroxybenzoic acid (114.9 μg/g sample), vanillin (63.7 μg/g sample), and ferulic acid (61.6 μg/g sample). In conclusion, extraction process may improve the processing, ulization and function of AB so that AB shows functional ingredients potential. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T04:19:14Z (GMT). No. of bitstreams: 1 ntu-100-R98641034-1.pdf: 2745236 bytes, checksum: a51b6bf5b4cd993b1b3db24dbdc8bb49 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 目錄
頁碼 摘要…………………………………………………………………....І Abstract…………………………………………………………………Ⅱ 縮寫對照表………………………………………………………………Ⅲ 目錄………………………………………………………………………Ⅴ 圖目錄……………………………………………………………………Ⅷ 表目錄…………………………………………………………………..Ⅸ 壹、前言…………………………………………………………………1 貳、文獻顧………………………………………………………………2 (一)薏苡簡介…………………………………………………………2 (二)薏仁麩皮的一般組成……………………………………………2 (三)薏仁麩皮及其萃取物的機能性研究……………………………5 (四)自薏仁麩皮中分離出來的植物化學成分………………………8 (五)反應曲面法簡介…………………………………………………11 (六)脂質之氧化作用…………………………………………………15 (七)影響脂質氧化之因素……………………………………………18 (八)萃取方法…………………………………………………………20 (九)萃取與酚類化合物………………………………………………21 (十)酚類化合物的機能性研究………………………………………24 (十一)膳食纖維的介紹………………………………………………29 參、研究動機與實驗架構………………………………………………33 (一)研究動機…………………………………………………………33 (二)實驗架構…………………………………………………………34 肆、實驗材料與方法……………………………………………………36 一、實驗材料…………………………………………………………36 (一)薏仁麩皮………………………………………………………36 (二)試驗藥品………………………………………………………36 (三)儀器設備………………………………………………………37 二、實驗方法…………………………………………………………38 (一)以RSM設計13組水洗薏仁麩皮的條件(預實驗)………………38 (二)尋找萃取條件(預實驗)………………………………………41 (三)RSM設計兩因子萃取實驗………………………………………44 (四)總酚含量的測定………………………………………………46 (五)總類黃酮含量的測定…………………………………………46 (六)製備HPLC分析的樣品…………………………………………47 (七)以HPLC分析樣品中酚類化合物的分析條件…………………48 (八)一般組成分析…………………………………………………49 三、統計分析…………………………………………………………51 伍、結果…………………………………………………………………52 一、以RSM設計水洗薏仁麩皮條件 (預實驗)…………………… 52 1.用水量和水洗時間對薏仁麩皮水洗液中總固形物含量的影響54 2. 用水量和水洗時間對薏仁麩皮水洗液中不可溶性固形物含量 的影響…………………………………………………………57 3. 用水量和水洗時間對薏仁麩皮水洗液中不可溶性固形物含量/ 總固形物含量比的影響………………………………………60 二、尋找萃取條件 (預實驗) ……………………………………61 1. 薏仁麩皮的用量對ABE1萃取率的影響………………………61 2. 乙醇濃度對ABE1萃取率的影響………………………………62 3. 萃取溫度和時間對ABE1萃取率的影響………………………62 4. 萃取溫度、時間、乙醇濃度和乙醇溶液體積對ABE1萃取率 的影響…………………………………………………………64 三、以反應曲面法選擇薏仁麩皮乙醇萃取物的萃取條件………67 1. 溫度和乙醇濃度對ABE1萃取率之影響………………………69 2. 乙醇濃度和萃取溫度對總酚含量之影響……………………74 3. 乙醇濃度和萃取溫度對總類黃酮含量之影響………………78 4. 最適萃取條件的選擇…………………………………………81 四、最適萃取條件酚類化合物含量………………………………82 1. 最適萃取條件下ABE1中產率、總酚和總類黃酮含量………82 2. 標準品之滯留時間與線性迴歸方程式………………………82 3.ABE1-Ea中酚類化合物含量……………………………………84 五、加工前後薏仁麩皮一般組成及膳食纖維含量之變化………88 1.一般組成含量之變化…………………………………………88 2.膳食纖維含量之變化…………………………………………90 陸、討論………………………………………………………………92 一、水洗薏仁麩皮對ABE1的影響…………………………………92 二、萃取酚類化合物相關因子對ABE1之影響……………………92 三、探討不同萃取物利用反應曲面法尋找最適萃取酚類化合物 條件的差異……………………………………………………93 四、ABE1-Ea和ABE-Ea之產率及萃取過程的差異…………………94 五、ABE1-Ea和ABE-Ea酚類化合物含量的差異……………………94 六、經過處理後的ABE1和薏仁麩皮的優勢………………………96 柒、結論…………………………………………………………………97 捌、參考文獻……………………………………………………………98 | |
dc.language.iso | zh-TW | |
dc.title | 以反應曲面法探討不同萃取條件對薏仁麩皮機能性成分的影響 | zh_TW |
dc.title | Effects of different extraction conditions on the functional components of adlay bran using response surface methodology | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 賴喜美(Hsi-Mei Lai),許輔(Fuu Sheu),龔瑞林(Zwe-Ling Kong),蘇南維(Nan-Wei Su) | |
dc.subject.keyword | 薏仁麩皮,反應曲面法,酚類化合物,萃取, | zh_TW |
dc.subject.keyword | adlay bran,response surface methodology,phenolic compounds,extraction, | en |
dc.relation.page | 108 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2011-08-18 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 食品科技研究所 | zh_TW |
顯示於系所單位: | 食品科技研究所 |
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