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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 謝文陽 | |
dc.contributor.author | Chun-Fu Lin | en |
dc.contributor.author | 林俊甫 | zh_TW |
dc.date.accessioned | 2021-06-08T04:16:34Z | - |
dc.date.copyright | 2010-08-04 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-08-02 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/22380 | - |
dc.description.abstract | 壬基苯酚(nonylphenol)為非離子型界面活性劑,廣泛運用在工業和家庭用途,會隨廢水排放到環境中。此化合物具有毒性,結構和動物體內的雌性激素相似,在生物體內會干擾正常內分泌系統運作,因而被視為環境荷爾蒙。本實驗的目的在於先從台灣沿岸海水,分離出具壬基苯酚降解能力的菌株,進而探討其特性。根據 16S rDNA 親緣分析得知具較強壬基苯酚降解活性的35 株分離株,可歸類
於氣單胞菌屬(Aeromonas )、弧菌屬(Vibrio)、交替單胞菌屬(Alteromonas)、沙雷菌屬(Shewanella )和擬交替單胞菌屬(Pseudoalteromonas)等屬。針對其中10株壬基苯酚降解能力較強的分離株,利用 HPLC 分析其培養七天的菌液,發現壬基苯酚剩降解為 20%~30%,由此看來,菌液中減少的壬基苯酚之苯環,應多已被 降解。 | zh_TW |
dc.description.abstract | Nonylphenol (NP) is a nonionic surfactant used in many industrial and domestic applications, belonging to a group of pollutants which are widely distributed in aquatic environment. NP is toxic and possessing the ability to disrupt the function of endocrine system by mimicking hormone, blocking or stimulating hormonal production. The objective of this study was to isolate and characterize the bacterial strains capable of degrading NP from coastal seawaters of Taiwan The phylogenetic analysis according to 16S rDNA sequences revealed that 35 strong degrading activity isolates belonged to genus Vibrio, Aeromonas, Alteromonas, Shewanella and Pseudoalteromonas, respectively. Through the test of NP removal, 10 isolates
exhibited the highest rate of degradation of NP, when compared with the other isolates. The results showed that NP was readily degraded by theses isolates with 20-30% of the total NP being removed within 7 days. It is apparent that NP should have already been degraded by these isolates | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T04:16:34Z (GMT). No. of bitstreams: 1 ntu-99-R97241203-1.pdf: 2088949 bytes, checksum: 4fee7fd56c113c1c7f3f8991c6957580 (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 英文摘要 ...............................................v
中文摘要.................................................vi 第一章 前言............................................. 1 第二章 材料和方法....................................... 4 第一節、海水樣本採集.................................... 4 第二節、培養基配製 (成份比例詳見附錄一、二)............. 4 第三節、水樣中異營細菌和壬基苯酚降解細菌的MPN 生菌數估算 4 第四節、分離、培養與純化菌株............................ 5 第五節、壬基苯酚濃度檢量線製備.......................... 5 第六節、壬基苯酚降解系統................................ 6 第七節、分離株形態與生理生化測試........................ 7 第八節、分離株之 16S rRNA 基因分析..................... 11 第九節、壬基苯酚降解能力測試........................... 14 第三章 結果............................................ 16 第一節、MPN 計數 ...................................... 16 第二節、壬基苯酚降解菌株的篩選與分離........................... 16 第三節、壬基苯酚降解細菌分離株 16S rRNA 基因的親緣分析及分群.... 16 第四節、壬基苯酚降解細菌分離株分離株形態與生理生化測試......... 17 第五節、各群 16S rRNA 基因序列分析.............................. 19 第六節、壬基苯酚降解能力測試................................... 21 第四章 討論........................................................ 23 第一節、MPN 計數 ............................................... 23 第二節、分離株之16S rDNA 分析與相近菌種........................ 23 第三節、分離株之壬基苯酚降解能力探討........................... 32 第四節、壬基苯酚降解性細菌探討................................. 33 第五章 總結........................................................ 35 參考文獻........................................................... 36 | |
dc.language.iso | zh-TW | |
dc.title | 壬基苯酚降解性海洋細菌分離株之特性研究 | zh_TW |
dc.title | Characterization of nonylphenol- degrading marine
bacterial isolates | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 湯森林,李重義,劉秀美,簡文達 | |
dc.subject.keyword | 界面活性劑,壬基苯酚,最大可能計數,生物降解,海洋細菌,氣單胞菌屬(Aeromonas ),弧菌屬(Vibrio),交替單胞菌屬(Alteromonas),沙雷菌屬(Shewanella ),擬交替單胞菌屬(Pseudoalteromonas), | zh_TW |
dc.subject.keyword | surfactant,nonylphenol (NP),most probable number (MPN),biodegradation,marine bacteria,Aeromonas,Vibrio,Alteromonas,Shewanella,Pseudoalteromonas, | en |
dc.relation.page | 69 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2010-08-02 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 海洋研究所 | zh_TW |
顯示於系所單位: | 海洋研究所 |
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