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標題: | 草莓苗期炭疽病之光波物理防治 Light wavelength controls anthracnose disease of strawberry seedling |
作者: | Jeng-Lin Wu 吳政霖 |
指導教授: | 沈湯龍 |
關鍵字: | 草莓,炭疽病,Colletotrichum siamense,Light-emitting diode (LED),紅光,藍光,混色光, Strawberry,Anthraconose,Colletotrichum siamense,Light-emitting diode (LED),Blue light,Red light,Gradient light, |
出版年 : | 2019 |
學位: | 碩士 |
摘要: | 草莓 (Fragaria x ananassa Duchessne) ,全臺種植面積達 550 公頃,於 2017 年 年產值近 10 億新台幣。草莓植株因台灣夏季高溫而無法越夏,農民需在隔年以走 莖苗重新進行定植。炭疽病 是 草莓 苗 期之 重大 病 害,造成 草 莓苗 的 大量 損 失,健 康 種苗 的 缺乏 成為 草 莓產 業 之嚴 重問 題。目前 農 民主 要仰 賴 殺菌 劑防 治 炭疽 病,然而 長年 的 殺菌 劑 使用 已導 致 炭疽 病 產生 抗藥 性,因此 需要 新 的防 治 手段 減少 殺 菌劑 之 使用 以及 維 持草 莓 苗之 健康。已有多篇 研究指不同光波長對植物病原有生長、繁殖抑制效果,且具有提升植物對病害抗性 之能力。因此本研究透過 200 PPFD 之不同光波長 (白光、紅光、2/3 紅光+1/3 藍 光、1/3 紅光+2/3 藍光、藍光) 照射在 PDA (Potato dextrose agar) 皿培之 Colletotrichum siamense、C. siamense 接種之草莓葉片及草莓苗、待克利上,尋找最 具有炭疽病抑制效果及草莓生長促進效果之光波長,並觀察光波長是否可以和農 藥同時使用,期望能作為草莓溫室育苗之物理防治策略。結果中發現與白光相比時, 1/3 紅光+2/3 藍光混和波長可抑制 C. siamense 14 %之菌絲生長、5%之產孢量以及 11%之孢子發芽率。同時 1/3 紅光+2/3 藍光波長也具有促進草莓防禦基因 Chitinase2 基因 1.3 倍之表現量之特性。1/3 紅光+2/3 藍光波長在實驗中也展現植株生長促 進之效果,與白光相比時其促進植株 1.1 倍葉片數、2.4 倍之走莖數及 1.3 倍鮮重 之生長。而所有波長皆不影響待克利之有效性及造成藥害。因 1/3 紅光+2/3 藍光具 有抑制 C. siamense 生長、促進草莓防禦基因表現以及促進草莓苗生長之特性,且 不會影響待克利之有效性,因此本研究提倡農民育草莓苗時給予 1/3 紅光+2/3 藍 光混和波長照射,以達到抑制病害並同時促進草莓苗生長之目標。 Strawberry is an important economic crop in Taiwan, the total planting area has reached 550 hectares, and the annual output value is nearly 1 billion NTD. However, due to the high temperature and humidity in Taiwan, strawberry cannot be planted continuously. Instead, strawberry farmers replant with strawberry runners annually. Anthracnose is one of the most severe diseases in the strawberry nursery industry, leading to a great loss on strawberry seedling. The lack of healthy seedlings has become a huge obstacle in the strawberry industry in Taiwan. Currently, farmers mainly rely on fungicides to prevent anthracnose, as a consequence, the massive use of fungicides has given rise to the anthracnose drug resistance. As a result, it is an emerging need of new methods for controlling this disease, in order to reduce the use of fungicides. Numerous studies have shown different light wavelengths capable of suppressing both plant pathogen growth and plant diseases. Here, in this study, we illuminated different light wavelengths (white light, red light, 2/3red+1/3blue, 1/3red+2/3blue, blue light) at 200 PPFD on Colletotrichum siamense on PDA (Potato dextrose agar) , inoculated strawberry leaves and seedlings, and co-treatment with difenoconazole, to obtain the optimal wavelength for controlling anthracnose disease, to promote strawberry seedlings growth, and to implement with difenoconazole, respectively. Our results showed that, in III compared to white light, 1/3red+2/3blue could inhibit 14% C. siamese mycelial growth, 5% of the sporulation and 11% of spore germination. In addition to C.siamense growth inhibition, 1/3red+2/3blue light could also augment the strawberry defense gene expressions, such as Chitinase-2 gene. The 1/3red+2/3blue light was also found to increase leave number, runner number, and overall fresh weight of seedlings than white light does. Furthermore, all light wavelengths would not impair difenoconazole’s effectiveness or enhance phytotoxicity. In conclusion, our study indicates that 1/3red+2/3blue light shows the characteristics of the growth inhibition of C. siamense, defense genes upregulation, and the growth of strawberry. Therefore, we recommend 1/3red+2/3blue light condition during the nursery period can be applied for suppressing anthracnose disease and promoting strawberry seedling growth. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/21755 |
DOI: | 10.6342/NTU201900544 |
全文授權: | 未授權 |
顯示於系所單位: | 植物醫學碩士學位學程 |
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