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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 呂廷璋,鄭光成 | |
| dc.contributor.author | Jin-Tong Yang | en |
| dc.contributor.author | 楊金銅 | zh_TW |
| dc.date.accessioned | 2021-06-08T03:17:32Z | - |
| dc.date.copyright | 2017-02-16 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-01-13 | |
| dc.identifier.citation | Agrawal, K.M.L., and Bahl, O.P. (1968). Glycosidases of phaseolus vulgaris II. isolation and general properties. J. Biol. Chem. 243, 103–111.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/21047 | - |
| dc.description.abstract | 羅漢果皂苷為一群具有高度甜味的三萜類糖苷分子，已經被證實具有廣泛的生理功能，分子結構上以帶有五個β-D-葡萄糖基的Mogroside V為主要天然羅漢果的皂苷形式。有文獻研究指出羅漢果皂苷為麥芽糖酵素抑制劑，可以有效地控制餐後血糖濃度的上升，在不同的羅漢果皂苷中，Mogroside IIIE較Mogroside V具有更好的抑制小腸麥芽糖酵素活力的功能。利用酵素催化的方法可將五醣皂苷Mogroside V轉化為Mogroside IIIE，然酵素生產成本高，酵素本身容易失活，且不能重複使用，因此在實際應用中具有一定的侷限性。本實驗利用固定化酵素的方式，將葡萄糖苷酵素固定於載體上進行Mogroside V的轉化反應，此一方法的重複利用性可克服酵素高昂生產成本的問題。實驗中利用HPLC-UV系統可以有效地分離和定量酵素反應系統中四種不同的羅漢果皂苷，進一步利用HPLC/ESI tandem MS建立對於羅漢果皂苷的定性分析方法。以此觀察到在酵素反應的實驗中Mogroside V含量逐漸下降；兩種類型的四醣皂苷Siamenoside I和Mogroside IV含量先增長後下降；而Mogroside IIIE（三個醣基）含量逐漸上升；結果顯示葡萄糖苷酵素可選擇性水解Mogroside V的β-1,6糖苷鍵結，並經由五醣皂苷到四醣皂苷，最後到三醣皂苷 (Mogroside IIIE) 的轉換過程。 | zh_TW |
| dc.description.abstract | Mogrosides, a group of triterpene glycosides from the fruit of Siraitia grosvenorii (Swingle), have been proved to have wide biological activities. Mogroside V (with five glucose residues) and mogroside IIIE (with three glucose residues) are the major components of mogrosides in Lo han ko fruit. It has been demonstrated that mogroside IIIE exerts anti-hypoglycemic effect by inhibiting intestinal maltase activity than mogroside V. In this study, enzymatic hydrolysis was utilized for the enrichment of mogroside IIIE by β-glucosidase from Aspergillus niger that can hydrolyze glycosidic bonds to release the non-reducing terminal glycosyl residues from glycosides and oligosaccharides. Nevertheless, suspended enzyme catalysis exhibits some limitations in practical applications by economic concerns. β-Glucosidase was then immobilized on solid carriers for the catalysis of mogroside V with reusability for reducing costs. During the enzymatic reaction, mogroside V was transformed to mogroside III E via siamenoside I and mogroside IV. The results indicated that the immobilized glucosidase system enables to selectively hydrolyze the β-1,6-glycosidic linkage of mogrosides. Moreover, we attempt to establish HPLC-ESI tandem MS analytical method for qualitative and quantitative analysis of mogrosides. The development of the immobilized enzyme system achieved the conversion of mogroside V into mogroside IIIE, which is capable of being further applied to prepare large scale of mogroside IIIE. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T03:17:32Z (GMT). No. of bitstreams: 1 ntu-106-R03641041-1.pdf: 9446622 bytes, checksum: 806d9107a3e78b6c3ed36706a1525c60 (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 摘要 i
Abstract ii 目錄 I 圖目錄 IV 表目錄 VI 壹、 前言 1 貳、 文獻回顧 2 2.1. 皂素 (Saponins) 2 2.1.1. 定義 2 2.1.2. 分類及特徵 2 2.1.3. 羅漢果皂苷 3 2.2. 羅漢果 4 2.2.1. 羅漢果皂苷化學結構 5 2.2.2. 羅漢果皂苷含量及甜味性質 6 2.2.3. 羅漢果皂苷之生理活性 7 2.2.3.1. 抗糖尿病 8 2.2.3.2. 抗氧化 8 2.2.3.3. 抗發炎 8 2.2.3.4. 抗致癌物 8 2.2.3.5. 抗人類皰疹病毒炎症 9 2.2.4. 分析方法 9 2.3. β-葡萄糖苷酵素 12 2.3.1. β-葡萄糖苷酵素的來源 12 2.3.2. β-葡萄糖苷酵素的生化特性 12 2.3.3. p-NPG作為酵素的反應底物 13 2.4. 酵素固定化 14 2.4.1. 酵素固定化方法 14 2.4.1.1. 吸附法 15 2.4.1.2. 共價結合法 15 2.4.1.3. 交聯法 16 2.4.1.4. 酵素包埋法 16 2.4.2. 固定載體材料 17 2.4.3. 酵素固定化應用 17 參、 材料與方法 19 3.1. 實驗目的 19 3.2. 實驗架構 20 3.3. 實驗材料 21 3.3.1. 羅漢果萃取物 21 3.3.2. 實驗藥品 21 3.3.3. 儀器設備 21 3.4. 實驗方法 23 3.4.1. 載體準備和活化 23 3.4.1.1. 尼龍載體 (Nylon pellets) 23 3.4.1.2. 纖維素顆粒 (Cellulose beads) 23 3.4.1.3. 玻璃微球 (Glass spheres) 23 3.4.2. 酵素固定 (Enzyme immobilization) 23 3.4.3. 蛋白質分析 (Protein assay) 24 3.4.4. 載體表面形態電鏡掃描分析 24 3.4.5. 載體表面化學元素和共價鍵分析 24 3.4.6. 固定化酵素活性檢定 25 3.4.7. 酵素反應pH測試 25 3.4.8. 酵素反應溫度測試 26 3.4.9. 酵素溫度穩定性測試 26 3.4.10. 酵素動力學參數測定 26 3.4.11. 羅漢果萃取物水溶液酵素水解反應 27 3.4.12. 水解液中葡萄糖含量的測定 27 3.4.13. 水解液中還原糖總量的測定 27 3.4.14. HPLC-UV 對羅漢果皂苷進行定量分析 28 3.4.14.1. 羅漢果皂苷的純化 28 3.4.14.2. 線性範圍、偵測極限與定量極限： 28 3.4.14.3. PE回收率和穩定性： 29 3.4.14.4. HPLC-UV精密度和準確度： 29 3.4.15. 羅漢果酵素水解樣品分析 30 3.4.16. LC-MSn 對羅漢果皂苷進行定性分析 30 3.4.17. 統計分析 31 肆、 結果與討論 32 4.1. 固定化葡萄糖苷酵素系統的建立 32 4.1.1. 固定酵素載體表面之微觀結構 32 4.1.2. 固定化酵素載體表面元素及化學鍵結分析 37 4.1.3. 葡萄糖苷酶酵素吸光值標準曲線製作 43 4.1.4. 三種載體固定之酵素含量測試 43 4.1.5. 對硝基苯吸光值標準曲線 46 4.1.6. 固定酵素系統反應活性測試 46 4.1.7. 固定化玻璃微球酵素系統反應pH和反應溫度測試 46 4.1.8. 固定化玻璃微球酵素系統反應動力學參數 51 4.1.9. 固定化玻璃微球酵素系統溫度穩定性測試 51 4.1.10. 固定化玻璃微球酵素系統貯存穩定性測試 52 4.1.11. 固定化玻璃微球酵素系統操作重複利用穩定性測試 52 4.2. 固定化酵素系統對於羅漢果皂苷之轉化 56 4.2.1. HPLC-UV對羅漢果皂苷進行分離和定量 56 4.2.2. 羅漢果皂苷之酵素水解 58 4.2.3. 羅漢果萃取物水溶液經由酵素反應之結果分析 63 4.2.4. 固定化玻璃微球酵素系統貯存穩定性測試 64 4.2.5. 運用固定化酵素系統對於羅漢果萃取物水溶液進行連續十個批次的水解反應之結果分析 64 4.2.6. 電噴灑游離串聯質譜對羅漢果皂苷的定性分析 70 伍、 結論與展望 83 陸、 參考文獻 84 柒、 附錄 91 7.1. 個人履歷 91 7.2. Q & A 92 7.3. Paper初稿 96 | |
| dc.language.iso | zh-TW | |
| dc.title | 固定化葡萄糖苷酵素應用於羅漢果皂苷去糖基之研究 | zh_TW |
| dc.title | Immobilized β-Glucosidase for Mogrosides Deglycosylation | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 蕭心怡,陳宏彰,陳寬宜 | |
| dc.subject.keyword | 羅漢果皂?,葡萄糖?酵素,固定化酵素,電噴灑游離串聯質譜,水解反應, | zh_TW |
| dc.subject.keyword | Mogrosides,β-Glucosidase,Immobilized enzyme,ESI tandem MS,Hydrolysis, | en |
| dc.relation.page | 112 | |
| dc.identifier.doi | 10.6342/NTU201700029 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2017-01-13 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 食品科技研究所 | zh_TW |
| 顯示於系所單位： | 食品科技研究所 | |
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| ntu-106-1.pdf 目前未授權公開取用 | 9.23 MB | Adobe PDF |
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