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Title: | 抗菌與基因傳遞應用之水性陽離子聚胺酯合成 Synthesis of water-based cationic polyurethane for antibacterial and gene delivery applications |
Authors: | Geng-Hsi Wu 吳庚熹 |
Advisor: | 徐善慧(Shan-hui Hsu) |
Keyword: | 可降解聚胺酯,陽離子,抗菌,基因轉染, Biodegradable polyurethane,cationic,antibacterial,transfection, |
Publication Year : | 2015 |
Degree: | 碩士 |
Abstract: | 陽離子高分子常作為抗菌材料與基因轉染劑用途,水性製程可減少環境污染並且避免產品中溶劑殘留所帶來的風險。本研究中,以聚己內酯、二異氰酸異佛爾酮及甲基二乙醇胺,在75°C下合成水性可降解陽離子聚胺酯。在酸性水溶液環境中劇烈攪拌,形成水性分散液。水性可降解陽離子聚胺酯均勻分散於水中形成大小為80奈米的奈米粒,其界面電位約60 mV。在倒入模具中成膜後,固體聚胺酯膜的接觸角為約67°,而聚胺酯膜的界面電位為約16 mV。水性陽離子聚胺酯奈米粒及薄膜均對大腸桿菌有良好的抗菌活性,尤其奈米粒的抗菌效果在與大腸桿菌接觸3小時後,即達到100%抗菌率,水性陽離子聚胺酯薄膜則於接觸24小時後達100%抗菌率。水性可降解陽離子聚胺酯奈米粒在濃度低於1000 μg/mL時,對HEK293T細胞無明顯細胞毒性,而作為基因轉染劑時,在聚胺酯/質體混合比為12.5/1及25/1下,亦無顯著細胞毒性。在以DsRed對HEK293T細胞進行基因轉染後,於48小時測得其轉染效率約為20%。 Cationic polymers are often used as antimicrobial materials and transfection reagents. Water-based process could reduce environmental pollution and prevent the risk of solvent residue in the final product. In this study, waterborne biodegradable cationic polyurethane (WCPU) was synthesized by reacting polycaprolactone (PCL diol), isophorone diisocyanate (IPDI), and N-methyldiethanolamine (N-MDEA) under 75°C. An aqueous dispersion of WCPU nanoparticles (NPs) could be acquired by vigorous stirring under acidic condition. The particles in the dispersion were ~80 nm in size with a zeta potential of ~60 mV. When cast into films, the contact angle of the film was ~67° and the zeta potential was ~16 mV. WCPU NPs demonstrated excellent antibacterial activity against Escherichia coli (100% inhibition with a contact time of 3 hours). Meanwhile, the antibacterial ratio of WCPU film reached 100% after 24 hours of contact. Moreover, WCPU NPs could be used as a transfection reagent without significant toxicity for concentration less than 1000 mg/mL and showed the ability to condensate plasmid DNA on the WCPU NPs. The transfection efficiency for HEK293T cells was ~20% at 48 h after the transfection. Therefore, the WCPU synthesized in this study has potential antibacterial and gene delivery applications. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/19784 |
Fulltext Rights: | 未授權 |
Appears in Collections: | 高分子科學與工程學研究所 |
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ntu-104-1.pdf Restricted Access | 15.57 MB | Adobe PDF |
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