請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17402
標題: | EB 病毒 BGLF4 透過調控細胞骨架以協助病毒細胞質組裝區域之形成 EBV BGLF4 Induces Formation of Viral Cytoplasmic Assembly Compartment Through Rearrangement of Cytoskeleton |
作者: | Tsung-Yu Yang 楊宗諭 |
指導教授: | 陳美如(Mei-Ru Chen) |
關鍵字: | EB 病毒,病毒細胞質組裝區域,BGLF4,IQGAP1,細胞骨架,微管,高基氏體, Epstein-Barr virus (EBV),cytoplasmic assembly compartment,BGLF4,IQGAP1,cytoskeleton,microtubule,Golgi apparatus, |
出版年 : | 2013 |
學位: | 碩士 |
摘要: | Epstein-Barr virus (EBV) is the first human virus to be implicated in carcinogenesis. Over 90 percent of people are infected by EBV in the world. Upon lytic replication, the nucleocapsids of EBV need to be translocated from the nucleus into cytoplasm for tegumentation and final maturation after DNA replication and encapsidation. Little is known about how EBV remodels cytoskeleton and cellular secretory apparatuses for efficient cytoplasmic assembly. Using EBV infected nasopharyngeal carcinoma NA cells we showed that, cells displayed modified nuclear structure after EBV reactivation. Viral capsid, tegument proteins and glycoproteins are clustered at juxtanuclear concave region accompanied by redistributed cytoplasmic organelles, MTOC and cytoskeleton regulator IQGAP1, suggesting EBV builds a specialized cytoplasmic assembly compartment for an efficient cytoplasmic maturation process. This compartment was disrupted after siRNA knockdown of BGLF4 kinase. Transient expression of BGLF4 induced a compact Golgi structure along with reorganization of MTOC and IQGAP1, indicating that BGLF4 plays a critical role for assembly compartment formation and cytoskeleton rearrangement. Treatment with microtubule-disturbing agents in cells inhibited BGLF4 induced compact Golgi structure, suggesting BGLF4 induces assembly compartment formation through regulation of cytoskeleton rearrangement. Since BGLF4 interacted with IQGAP1 in co-immunoprecipitation assay and
phosphorylated IQGAP1 in vitro,it is possible that BGLF4 regulates cytoskeleton through phosphorylation of IQGAP1. While viral DNA replication and maturation was enhanced in IQGAP1 knockdown cells, IQGAP1 may serve as a negative regulator of EBV lytic cycle. Taken together, I propose that BGLF4 induces cytoskeleton rearrangement and instability through phosphorylation of IQGAP1, and subsequently facilitates assembly compartment formation and virus maturation. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17402 |
全文授權: | 未授權 |
顯示於系所單位: | 微生物學科所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-102-1.pdf 目前未授權公開取用 | 19.24 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。