增強多光子螢光訊號顯微鏡

Abstract

高強度的激發光場會產生光破壞，也就是光漂白效應，已經是生物科技裡的長久大問題。在顯微鏡系統下，這效應會強烈的破壞樣品。現在，我們利用一個特殊的元件取代蓋玻片，增強激發與收光的效率，最高達到20倍的增益，同時解決光漂白這大問題。利用一維光子晶體引入液晶缺陷層為此元件的新穎結構。與過去的強化激發振幅致螢光放大研究相比，我們利用元件縮短激發雷射的脈衝，在同功率下使多光子激發機率大幅提升。此元件擁有兩種模態：可調控螢光增強與強烈螢光增強型，使得此種元件在生物醫學影像科技上開啟新的一頁。

The photobleaching effect is the photochemical destruction of a fluorophore by high-intensity light, which has long been a nuisance in the field of biotechnology. This undesired effect damages the bio-sample intensively, as often encountered in multiphoton microscopy. Here, we demonstrate a unique cover glass-like device to solely enhancing the fluorescence excitation and detection, achieving a 20-fold maximum gain without photobleaching, by means of a one-dimensional photonic crystal infiltrated with liquid crystal as a central defect layer. In comparison with the typical fluorescence enhancement techniques involving the excitation amplification proposed previously, our method, characterized by the compression of excitation pulse instead, generates the higher excitation possibility. The photonic bandgap structure employed in this approach possesses two modes: tunable fluorescence enhanced and strong fluorescence enhanced, enabling such devices to find a place in bio-imaging technologies.