脊椎組織拉曼頻譜分析

Abstract

硬脊膜外置入術常用於麻醉及止痛。目前臨床上用空針裡的空氣或生理食鹽水的壓力測試到達硬脊膜外空間。到達硬脊膜外空腔會有阻力消失。此用空氣或水達到阻力消失的方法有一定的失敗率。此篇論文利用拉曼光譜技術來分析脊椎組織，進一步辨別各組織。脊椎組織的原始拉曼光譜會先作小波轉換及多級數基線擬合，以去除高頻雜訊及低頻螢光，再作進一步分析比較。經過訊號處理的脊椎組織拉曼光譜，在 δ(CH2)，胺基III，胺基I及細胞膜相關分子的波高有不同的表現。進一步需研發拉曼光纖的探針，以利於臨床使用。

Anesthesia and analgesia can be done with drug injecting into epidural space through epidural approach. Frequently this is done under loss of resistance method using air or normal saline. However, there is certain rate of failure rate with this method. Thus, we proposed use of Raman spectrum technique to analyze and differentiate the spinal tissues. The raw Raman spectrum was first preprocessed with wavelet transform and polynomial baseline fitting to remove the high frequency noise and low frequency fluorescence. The processed Raman spectrum of the spinal tissues showed different peak intensity and contours in regions of δ(CH2), amide III, amide I and region related to membrane molecules. Further research in fiberoptic Raman probe is needed to incorporate the probe into Tuohy needle and making the clinical differentiation of spinal tissues with Raman spectrum come to reality.