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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 高文媛(Wen-Yuan Kao) | |
dc.contributor.author | Cheng-Tai Huang | en |
dc.contributor.author | 黃承泰 | zh_TW |
dc.date.accessioned | 2021-05-12T09:34:48Z | - |
dc.date.available | 2018-05-31 | |
dc.date.available | 2021-05-12T09:34:48Z | - |
dc.date.copyright | 2018-05-31 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-05-15 | |
dc.identifier.citation | Adams MA, Turnbull TL, Sprent JI, Buchmann N (2016) Legumes are different: Leaf nitrogen, photosynthesis, and water use efficiency. Proc. Natl. Acad. Sci. 113:4098-4103
Appunu C, N’Zoue A, Moulin L, Depret G, Laguerre G (2009) Vigna mungo, V. radiata and V. unguiculata plants sampled in different agronomical–ecological–climatic regions of India are nodulated by Bradyrhizobium yuanmingense. Syst. Appl. Microbiol.32:460-470 Andam CP, Parker MA (2008) Origins of Bradyrhizobium nodule symbionts from two legume trees in the Philippines. J. Biogeogr. 35:1030-1039 Aserse AA, Räsänen LA, Aseffa F, Hailemariam A, Lindström K (2012) Phylogenetically diverse groups of Bradyrhizobium isolated from nodules of Crotalaria spp., Indigofera spp., Erythrina brucei and Glycine max growing in Ethiopia. Mol. Phylogenet. Evol. 65:595-609 Azevedo H, Lopes FM, Silla PR, Hungria M (2015) A database for the taxonomic and phylogenetic identification of the genus Bradyrhizobium using multilocus sequence analysis. BMC Genomics 16(Suppl 5):S10 Barrett CF, Parker MA (2005) Prevalence of Burkholderia sp. nodule symbionts on four mimosoid legumes from Barro Colorado Island, Panama. Syst. Appl. Microbiol. 28:57-65 Bergersen FJ, Turner GL, Amarger N, Mariotti F, Mariotti A (1986) Strain of Rhizobium lupini determines natural abundance of 15N in root nodules of Lupinus spp. Soil. Biol. Biochem. 18:97-101 Bergersen FJ, Peoples MB, Turner GL (1988) Isotopic discrimination during the accumulation of nitrogen by soybeans. Aust. J. Plant Physiol. 15:407-420 Binggeli P (1996) A taxonomic, biogeographical and ecological overview of invasive woody plants. J. Veg. Sci. 7:121-124 Brueck H (2008) Effects of nitrogen supply on water-use effeciency of higher plants. J. Plant Nutr. Soil Sci. 171:210-219 Burdon JJ, Gibson AH, Searle SD, Woods MJ, Brockwell J (1999) Variation in the effectiveness of symbiotic associations between native rhizobia and temperate Australian Acacia: within-species interactions. J. Appl. Ecol. 36:398-408 Chen WM, Lee TM, Lan CC, Cheng CP (2000) Characterization of halotolerant rhizobia isolated from root nodules of Canavalia rosea from seaside areas. FEMS Microbiol. Ecol. 34:9-16 Chen WM, Lee TM (2001) Genetic and phenotypic diversity of rhizobial isolates from sugarcane-Sesbania cannabina-rotation fields. Biol. Fertil. Soils 34:14-20 Chen WM, Moulin L, Bontemps C, Vandamme P, Bena G, Boivin-Masson C (2003) Legume symbiotic nitrogen fixation by β-Proteobacteria is widespread in nature. J. Bacteriol. 185:7266-7272 Chen WM, James EK, Chou JH, Sheu SY, Yang SZ, Sprent JI (2005) Beta-rhizobia from Mimosa pigra, a newly discovered invasive plant in Taiwan. New Phytol. 168:661-675 Chen WM et al. (2006) Burkholderia mimosarum sp. nov., isolated from root nodules of Mimosa spp. from Taiwan and South America. Int. J. Syst. Evol. Microbiol. 56:1847-1851 Chiang MY, Hsu LM, Yuan CI, Chen FY, Chiang YJ (2003) The harmful effect and ecology of invasive plants in Taiwan The Harmful Effect and Field Management of Mikania micrantha, 97-109. Weed Science Society of the Republic of China and Hualien District Agricultural Research and Extension Station, Council of Agricultural Executive Yuan, Hualien Cole MA, Elkan GH (1973) Transmissible Resistance to Penicillin G, Neomycin, and Chloramphenicol in Rhizobium japonicum. Antimicrob. Agents Chemother. 4:248-253 Corby HDL (1988) Types of rhizobial nodules and their distribution among the Leguminosae. Kirkia 13:53-124 Crisóstomo JA, Rodríguez-Echeverría S, Freitas H (2013) Co-introduction of exotic rhizobia to the rhizosphere of the invasive legume Acacia saligna, an intercontinental study. Appl. Soil Ecol. 64:118-126 Cronk QCB, Fuller JL (1995) Plant Invaders: The Threat to Natural Ecosystems. Chapman & Hall, London. Dart PJ, Mercer FV (1966) Fine structure of bacteroids in root nodules of Vigna sinensis, Acacia longifolia, Viminaria juncea, and Lupinus angustifolius. J. Bacteriol. 91:1314-1319 Doyle JJ, Luckow MA (2003) The rest of the iceberg. Legume diversity and evolution in a phylogenetic context. Plant Physiol. 131:900-910 Doyle JJ (2011) Phylogenetic perspectives on the origins of nodulation. Mol. Plant-Microbe Interact. 24:1289-1295 Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput Nucleic Acids Res. 32:1792-1797 Elliott GN et al. (2007) Nodulation of Cyclopia spp. (Leguminosae, Papilionoideae) by Burkholderia tuberum. Ann. Bot. 100:1403–1411 Grönemeyer JL, Kulkarni A, Berkelmann D, Hurek T, Reinhold-Hurek B (2014) Rhizobia indigenous to the Okavango region in sub-Saharan Africa: diversity, adaptations, and host specificity. Appl. Environ. Microbiol. 80:7244-7257 Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0.'. Systematic Biology 59:307-321 Gyaneshwar P et al. (2011) Legume-nodulating betaproteobacteria: Diversity,host range, and future prospects. Mol. Plant-Microbe Interact. 24:1276-1288 Haag AF et al. (2013) Molecular insights into bacteroid development during Rhizobium–legume symbiosis. FEMS Microbiol Rev 37:364-383 Hall TA (1999) BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41:95-98 Hanelt P (2001) Leguminosae. In: Hanelt P and Institute of plant genetics and crop plant research (ed) Mansfeld's encyclopedia of agricultural and horticultural crops (excepts ornamentals). Springer-Verlag, Berlin Holm LG, Pancho JV, Herberger JP, Plucknett DL (1991) A geographical atlas of world weeds. Krieger Publisher Company, Malabar, Florida, USA Horn K, Parker IM, Malek W, Rodríguez-Echeverría S, Parker MA (2014) Disparate origins of Bradyrhizobium symbionts for invasive populations of Cytisus scoparius (Leguminosae) in North America. FEMS Microbiol. Ecol.:1-10 Huang TC (1993) Flora of Taiwan, vol. 3. 2nd edn. Editorial Committee of the Flora of Taiwan, Department of Botany, National Taiwan University, Taipei, Taiwan. Huang CT, Liu CT, Chen SJ, Kao WY (2016) Phylogenetic identification, phenotypic variation and symbiotic characteristics of a peculiar rhizobium, strain CzR2, isolated from Crotalaria zanzibarica in Taiwan. Microbes Environ. Huang CT, Liu CT, Kao WY (2018) Rhizobia symbiosis of seven leguminous species growing along Xindian riverbank of Northern Taiwan. Taiwania 68:7-15 Hung MH, Bhagwath AA, Shen FT, Devasya RP, Young CC (2005) Indigenous rhizobia associated with native shrubby legumes in Taiwan. Pedobiologia 49:577-584 Huson DH, Bryant D (2006) Application of phylogenetic networks in evolutionary studies. Mol. Biol. Evol. 23:254-267 Kalita M, Stpękowski T, Łotocka B, Małek W (2006) Phylogeny of nodulation genes and symbiotic properties of Genista tinctoria bradyrhizobia. Arch Microbiol 186:87-97 Kohl DH, Reynolds PHS, Shearer G (1989) Distribution of 15N within pea, lupin, and soybean nodules. Plant Physiol. 90:420-426 Koppell JH, Parker MA (2012) Phylogenetic clustering of Bradyrhizobium symbionts on legumes indigenous to North America. Microbiology 158:2050-2059 Lavin M, Herendeen PS, Wojciechowski MF (2005) Evolutionary rates analysis of Leguminosae implicates a rapid diversification of lineages during the tertiary. Syst. Biol. 54:575-594 Lewis G, Schrire B, Mackinder B, Lock M (2005) Legumes of the world. London: Royal Botanic Gardens Kew. Librado P, Rozas J (2009) DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451-1452 Liu XY, Wang ET, Li Y, Chen WX (2007) Diverse bacteria isolated from root nodules of Trifolium, Crotalaria and Mimosa grown in the subtropical regions of China. Arch Microbiol 188:1-14 Liu CT et al. (2011) Involvement of the Azorhizobial chromosome partition gene (parA) in the onset of bacteroid differentiation during Sesbania rostrata stem nodule development. Appl. Environ. Microbiol. 77:4371-4382 Justice SS, Hunstad DA, Cegelski L, Hultgren SJ (2008) Morphological plasticity as a bacterial survival strategy. Nature 6:162-168 Klock MM, Barrett LG, Thrall PH, Harms KE (2015) Host promiscuity in symbiont associations can influence exotic legume establishment and colonization of novel ranges. Divers. Distrib. 21: 1193-1203. Marchesi JR et al. (1998) Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl. Environ. Microbiol. 64:795-799 Mack RN (1997) Plant invasions: Early and continuing expressions of global change. Springer-Verlag, Berlin. Melkonian R et al. (2014) The geographical patterns of symbiont diversity in the invasive legume Mimosa pudica can be explained by the competitiveness of its symbionts and by the host genotype. Environ. Microbiol. 16:2099-2111 Menna P, Barcellos FG, Hungria M (2009) Phylogeny and taxonomy of a diverse collection of Bradyrhizobium strains based on multilocus sequence analysis of the 16S rRNA gene, ITS region and glnII, recA, atpD and dnaK genes. Int. J. Syst. Evol. Microbiol. 59:2934-2950 Menna P, Hungria M (2011) Phylogeny of nodulation and nitrogen-fixation genes in Bradyrhizobium: supporting evidence for the theory of monophyletic origin, and spread and maintenance by both horizontal and vertical transfer. Int. J. Syst. Evol. Microbiol. 61:3052-3067 Mergaert P et al. (2006) Eukaryotic control on bacterial cell cycle and differentiation in the Rhizobium–legume symbiosis. Proc. Natl. Acad. Sci. 103:5230-523 Moulin L, Bena G, Boivin-Masson C, Stępkowski T (2004) Phylogenetic analyses of symbiotic nodulation genes support vertical and lateral gene co-transfer within the Bradyrhizobium genus. Mol. Phylogenet. Evol. 30:720-732 Muñoz V, Ibanez F, Tonelli ML, Valetti L, Anzuay MS, Fabra A (2011) Phenotypic and phylogenetic characterization of native peanut Bradyrhizobium isolates obtained from Córdoba, Argentina. Syst. Appl. Microbiol. 34:446-452 Noisangiam R et al. (2012) Genetic diversity, symbiotic evolution, and proposed infection process of Bradyrhizobium strains isolated from root nodules of Aeschynomene americana L. in Thailand. Appl. Environ. Microbiol. 78:6236-6250 Oono R, Denison RF, Kiers ET (2009) Controlling the reproductive fate of rhizobia: how universal are legume sanctions? New Phytol. 183:967-979 Oono R, Denison RF (2010) Comparing symbiotic efficiency between swollen versus nonswollen rhizobial bacteroids. Plant Physiol. 154:1541-1548 Oono R, Schmitt I, Sprent JI, Denison RF (2010) Multiple evolutionary origins of legume traits leading to extreme rhizobial differentiation. New Phytol. 187:508-520 Parker MA, Malek W, Parker IM (2006) Growth of an invasive legume is symbiont limited in newly occupied habitats. Divers. Distrib. 12:563-571 Parker MA (2012) Legumes select symbiosis island sequence variants in Bradyrhizobium. Mol Ecol. 21:1769-1778 Peterson BJ, Fry B (1987) Stable isotopes in ecosystem studies. Annu. Rev. Ecol. Syst. 18:293-320 Pimentel D, Zuniga R, Morrison D (2005) Update on the environmental and economic costs associated with alien-invasive species in the United States. Ecol. Econ. 52:273-288 Polhill RM (1982) Crotalaria in Africa and Madagascar., Balkema, A. A., Rotterdam, The Netherlands. Pyšek P (1998) Is there a taxonomic pattern to plant invasions? Oikos 82:282-294 Reding HK, Lepo JE (1989) Physiological characterization of dicarboxylate-induced pleomorphic forms of Bradyrhizobium japonicum. Appl. Environ. Microbiol. 55:666-671 Richardson DM, Pyšek P, Rejmánek M, Barbour MG, Panetta FD, West CJ (2000a) Naturalization and invasion of alien plants: concepts and definitions. Divers. Distrib. 6:93-107 Richardson DM, Allsopp N, D'Antonio CM, Milton SJ, Rejmanek M (2000b) Plant invasions - the role of mutualisms. Biol. Rev. 75:65-93 Rivas R, Martens M, Lajudie Pd, Willem A (2009) Multilocus sequence analysis of the genus Bradyrhizobium. Syst. Appl. Microbiol. 32:101-110 Robinson D (2001) δ15N as an integrator of the nitrogen cycle. Trends Ecol. Evol. 16:153-162 Rodríguez-Echeverría S (2010) Rhizobial hitchhikers from Down Under: invasional meltdown in a plant–bacteria mutualism? J. Biogeogr. 37:1611–1622 Ronquist F et al. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 61:539-542 Sajnaga E, Małek W, Łotocka B, Stpkowski T, Legocki A (2001) The root-nodule symbiosis between Sarothamnus scoparius L. and its microsymbionts. Antonie van Leeuwenhoek 79:385–391 Samba R, De Lajudie P, Gillis M, Neyra M, Barreto M, Dreyfus B (1999) Diversity of rhizobia nodulating Crotalaria spp. from Senegal. Symbiosis 27:259-268 Shearer G, Kohl D, Harper JE (1980) Distribution of 15N among plant parts of nodulating and non-nodulating isolines of soybeans. Plant Physiol. 66:57-60 Shearer G et al. (1982) 15N abundance of nodules as an indicator of N metabolism in N2-fixing plants. Plant Physiol. 70:465-465 Skinner FA, Roughley RJ, Chandler MR (1977) Effect of yeast extract concentration on viability and cell distortion in Rhizobium spp. J. Appl. Bacteriol. 43:287-297 Sibuet JC, Hsu SK (1997) Geodynamics of the Taiwan arc-arc collision. Tectonophysics 274:221-251 Silva FV et al. (2014) Bradyrhizobium manausense sp. nov., isolated from effective nodules of Vigna unguiculata grown in Brazilian Amazonian rainforest soils. Int. J. Syst. Evol. Microbiol. 64:2358-2363 Sprent JI (2001) Nodulation in legumes. London: Royal Botanic Gardens Kew. Sprent JI (2007) Evolving ideas of legume evolution and diversity: a taxonomic perspective on the occurrence of nodulation. New Phytol. 174:11-25 Sprent JI (2008) 60Ma of legume nodulation. What’s new? What’s changing? J. Exp. Bot. 59:1081-1084 Sprent JI (2009) Legume nodulation: a global perspective. Wiley-Blackwell, Oxford, United Kingdom Steenkamp ET, Stępkowski T, Przymusiak A, Botha WJ, Law IJ (2008) Cowpea and peanut in southern Africa are nodulated by diverse Bradyrhizobium strains harboring nodulation genes that belong to the large pantropical clade common in Africa. Mol. Phylogenet. Evol. 48:1131-1144 Steinberger RE, Allen AR, Hansma HG, Holden PA (2002) Elongation correlates with nutrient deprivation in Pseudomonas aeruginosa unsaturated biofilms Microb. Ecol. 43:416-423 Stępkowski T, Moulin L, Krzyżańska A, McInnes A, Law IJ, Howieson J (2005) European origin of Bradyrhizobium populations infecting Lupins and Serradella in soils of Western Australia and South Africa. Appl. Environ. Microbiol. 71:7041-7052 Stępkowski T et al. (2007) Diversification of lupine Bradyrhizobium strains: Evidence from nodulation gene trees. Appl. Environ. Microbiol. 73:3254-3264 Stępkowski T, Watkin E, McInnes A, Gurda D, Gracz J, Steenkamp ET (2012) Distinct Bradyrhizbium communities nodulate legumes native to temperate and tropical monsoon Australia. Mol. Phylogenet. Evol. 63:265-277 Sy A et al. (2001) Methylotrophic Methylobacterium bacteria nodulate and fix nitrogen in symbiosis with legumes. J. Bacteriol. 183:214-220 Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30:2725-2729 Thies JE, Singleton PW, Bohlool BB (1991) Modeling symbiotic performance of introduced rhizobia in the field by use of indices of indigenous population size and nitrogen status of the soil. Appl. Environ. Microbiol. 57:29-37 Thrall PH, Burdon JJ, Woods MJ (2000) Variation in the effectiveness of symbiotic associations between native rhizobia and temperate Australian legumes: interactions within and between genera. J. Appl. Ecol. 37:52-65 Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680 Unkovich M (2013) Isotope discrimination provides new insight into biological nitrogen fixation. New Phytol. 198:643-646 Urtz BE, Elkan GH (1996) Genetic diversity among Bradyrhizobium isolates that effectively nodulate peanut (Arachis hypogaea). Can. J. Microbiol. 42:1121-1130 Van de Velde W et al. (2010) Plant peptides govern terminal differentiation of bacteria in symbiosis. Science 327:1122-1126 Vincent JM (1970) A manual for the practical study of the root-nodule bacteria., International biological programme handbook 15. Blackwell Scientific Publications, Oxford, United Kingdom. Vinuesa P et al. (2008) Multilocus sequence analysis for assessment of the biogeography and evolutionary genetics of four Bradyrhizobium species that nodulate soybeans on the asiatic continent. Appl. Environ. Microbiol. 74:6987-6996 Virginia RA, Delwiche CC (1982) Natural 15N abundance of presumed N2-fixing and non-N2-fixing plants from selected ecosystems. Oecologia 54:317-325 Vitousek PM (1990) Biological invasions and ecosystem processes: towards an integration of population biology and ecosystem studies Oikos 57:7-13 Vitousek PM, Howarth RW (1991) Nitrogen limitation on land and in the sea: how can it occur? Biogeochemistry 13:87-115 Vitousek PM, Mooney HA, Lubchenco J, Melillo JM (1997) Human domination of Earth's ecosystems. Science 277:494-499 Vitousek PM et al. (2002) Towards an ecological understanding of biological nitrogen fixation. Biogeochemistry 57:1-45 Wainwright M (1997) Extreme pleomorphism and the bacterial life cycle: a forgotten controversy. Perspectives in Bio. Med. 40:407-414 Wanek W, Arndt S (2002) Difference in delta(15)N signatures between nodulated roots and shoots of soybean is indicative of the contribution of symbiotic N(2) fixation to plant N. J. Exp. Bot. 53:1109-1118 Wang JY et al. (2013) Bradyrhizobium daqingense sp. nov., isolated from soybean nodules. Int. J. Syst. Evol. Microbiol. 63:616-624 Wang R et al. (2013) Bradyrhizobium arachidis sp. nov., isolated from effective nodules of Arachis hypogaea grown in China. Syst. Appl. Microbiol. 36:101-105 Williamson M (1997) Biological Invasions. Chapman& Hall, London. Wu SH, Chaw SM, Rejmánek M (2003) Naturalized Fabaceae (Leguminosae) species in Taiwan: the first approximation. Bot. Bull. Acad. Sin. 44:59-66 Wu SH, Rejmánek M, Grotkopp E, Ditomaso JM (2005) Herbarium records, actual distribution, and critical attributes of invasive plants: genus Crotalaria in Taiwan. Taxon 54:133-138 Wu SH, Yang TYA, Teng YC, Chang CY, Yang KC, Hsieh CF (2010) Insights of the latest naturalized flora of Taiwan: change in the past eight years. Taiwania 55:139-159 Xu LM, Ge C, Cui Z, Li J, Fan H (1995) Bradyhizobium liaoningense sp. nov., isolated from the root nodules of soybeans. Int. J. Syst. Bacteriol. 45:706-711 Yao ZY, Kan FL, Wang ET, Wei GH, Chen WX (2002) Characterization of rhizobia that nodulate legume species of the genus Lespedeza and description of Bradyrhizobium yuanmingense sp. nov. Int. J. Syst. Evol. Microbiol. 52:2219-2230 Yelenik SG, Stock WD, Richardson DM (2004) Ecosystem level impacts of invasive Acacia saligna in the South African fynbos. Restor. Ecol. 12:44-51 Yoneyama T, Uchiyama T, Yazaki J (1991) Ontogenetic change of nitrogen accumulation and natural 15N abundance in pea and faba bean with special reference to estimate of N2 fixation and 15N enrichment of nodules. J. Mass Spectrom Soc. Jpn. 39:267-276 Young JPW, Haukka KE (1996) Diversity and phylogeny of rhizobia. New Phytol. 133:87-94 Young KD (2006) The selective value of bacterial shape. Microbiol. Mol. Biol. Rev. 70:660-703 Zhang YF et al. (2008) Bradyrhizobium elkanii, Bradyrhizobium yuanmingense and Bradyrhizobium japonicum are the main rhizobia associated with Vigna unguiculata and Vigna radiata in the subtropical region of China. FEMS Microbiol. Lett. 285:146-154 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/handle/123456789/1244 | - |
dc.description.abstract | 南美豬屎豆 (Crotalaria zanzibarica Benth.) 是台灣歸化豆科植物中分布最廣的一種,為多年生的木本植物,常見於道路旁、河岸及廢耕地。野外觀察發現南美豬屎豆普遍具有根瘤,因此推測與固氮菌(根瘤菌)共生有助其在貧瘠棲地建立族群,是讓此植物能夠在台灣廣泛分布的原因之一。然而,南美豬屎豆在台灣的共生根瘤菌未曾被研究。本論文探討南美豬屎豆與根瘤菌之間的共生關係,以了解此外來植物在台灣的共生根瘤菌之多樣性及可能來源,並檢驗不同根瘤菌株對植物的共生表現。
從種植在台大溫室的南美豬屎豆根瘤中分離出具多形態的菌株CzR2,分析此菌株之6個管家基因 (atpD、dnaK、glnII、gyrB、recA 和 rpoB) 序列,結果顯示CzR2屬於Bradyrhizobium arachidis。CzR2在游離狀態時受到甘露醇或果糖誘導會產生多形態細胞,此現象首次在根瘤菌中被發現。CzR2在與南美豬屎豆共生時也產生多形態的類菌體,然而有些類菌體其染色體具多倍體,有別於游離細胞的單倍體及二倍體。此結果顯示CzR2與南美豬屎豆共生時其染色體有內複製的現象。 調查新店溪沿岸的南美豬屎豆及常見的六種共域豆科植物,這些植物具有不同的根瘤(有限及無限)和類菌體(膨大及非膨大)形態。由分離菌株的16S rRNA序列分析,得知南美豬屎豆、蠅翼草、異葉山螞蝗和鍊夾豆皆與Bradyrhizobium建立共生,與田菁共生的根瘤菌為Neorhizobium和Rhizobium,白花菽草則為Rhizobium,含羞草的共生根瘤菌為Cupriavidus和Paraburkholderia。雖然這些植物具有不同的共生特徵,其葉片都具有類似的穩定性氮同位素比值(δ15N),其值約為 -1 ‰;且這些植物根瘤的δ15N皆為正值(3.7-7.3 ‰),其中無限根瘤比有限根瘤普遍具有較高的δ15N數值。 以多基因序列 (dnaK-glnII-recA-rpoB) 分析從台灣北、中、南三河岸南美豬屎豆族群所分離出之59株根瘤菌株,以及其他共域豆科植物族群所分離出之54株根瘤菌株,結果顯示這些菌株皆屬於Bradyrhizobium且可區分成21個支序群。同時比較分析其他共域豆科的根瘤菌群時,發現某些菌群似乎對南美豬屎豆有專一性,然而某些菌群則廣泛出現在多種植物的根瘤中。多數南美豬屎豆根瘤菌具有代表美洲起源的nodA共生基因,其次為亞洲起源及世界廣布型,顯示此物種在台灣能夠與來自不同地理區的的根瘤菌建立共生。 藉由溫室實驗比較接種不同菌株對南美豬屎豆的影響,結果發現南美豬屎豆與不同菌株共生時產生不同的根瘤且類菌體形態亦有所不同,顯示根瘤及類菌體形態亦受到共生菌株的影響,不全受到宿主決定;南美豬屎豆植株生物量及植株總氮量在不同菌株接種處裡下有顯著差異,然而,南美豬屎豆植株生物量分配、氮含量、穩定性同位素比值和共生效率(總生物量變化/總根瘤生物量變化)受不同接種菌株的影響並不顯著。 綜合野外調查和溫室實驗結果,證實南美豬屎豆能夠與多樣Bradyrhizobium菌種建立有效共生,顯示其為廣適性宿主,此特徵有助於該物種擴散到不同地點時能找到相容根瘤菌。另一方面,來自南美洲的外來根瘤菌對於南美豬屎豆在台灣與根瘤菌建立共生中扮演重要角色。 | zh_TW |
dc.description.abstract | Crotalaria zanzibarica Benth., a perennial shrub native to Africa, is the most widely-distributed naturalized legume in Taiwan. The plant, commonly distributed along roadsides and riverbanks, and in abandoned fields, established symbiosis with rhizobia forming root nodules. Root nodules are capable of fixing nitrogen. Accordingly, the symbiosis with nitrogen-fixing rhizobia might help C. zanzibarica colonizing nutrient-poor habitats. In this dissertation, I studied the symbiotic relationship between rhizobia and this legume, aiming to understand the diversity and possible origins of the symbiotic rhizobia and the beneficial effects of the symbiosis to C. zanzibarica.
A rhizobial strain, designed as CzR2, was isolated from the nodules of C. zanzibarica grown in a greenhouse. This strain displayed pleomorphism, cell size ranging from 2 to 10μm, in free-living state when cultivated in standard YEM medium which significantly differs from any known rhizobia. Based on the analysis of atpD-dnaK-glnII-gyrB-recA-rpoB gene set, CzR2 belongs to Bradyrhizobium arachidis. Results of further experiments revealed that pleomorphism in this strain in its free-living state could be induced by mannitol, or fructose, but not by glucose. Accordingly, the pleomorpism is substrate-dependent. CzR2 in its free-living state contained haploid and diploid cells, while that in symbiosis with C. zanzibarica was elongated with polyploidy, suggesting the occurrence of genomic endo-reduplication. Legume-rhizobia symbioses of C. zanzibarica and six common legume species growing sympatrically along Xindian riverbank were investigated in Chapter 2. I found that these legumes form either determinate or indeterminate types of root nodules and harbored swollen or non-swollen bacteroids. Based on the 16S rRNA sequences, the symbionts of these legumes were classified as Bradyrhizobium, Neorhizobium, Rhizobium, Cupriavidus and Paraburkholderia. Irrespective of their possessing of diverse symbiotic traits and nodule symbionts, the seven legume species had similar and consistently negative leaf δ15N values (mean of -1.2 ‰), and showed 15N enrichment (varying from 3.7 to 7.3 ‰) in their nodules. In addition, variations in the values of leaf δ13C (varying from -29 to -34‰) among the seven legumes were measured, indicating their photosynthetic water use efficiencies were different. The results also suggested that C. zanzibarica could be nodulated by diverse rhizobia. To compare the symbionts of C. zanzibarica and sympatric legumes growing along three distant riverbanks in Taiwan, I collected 59 isolates from this plant and 54 isolates from coexisting legumes. Based on the multilocus sequence analysis of concatenated dnaK-glnII-recA-rpoB gene sequences, the C. zanzibarica isolates were highly diverse, belonging to 14 clades and varied among sampling sites, which can be either phylogenetically similar to or distinct from the isolates of coexisting legumes. The majority of C. zanzibarica isolates had nodA genes of American origin, following by Asian origin, while others might be cosmopolitan. To confirm the field isolates are able to nodulate C. zanzibarica and to compare the effects of symbionts on growth of this plant, I conducted single-strain inoculation experiment and investigated growth response, nodulation response, symbiotic efficiency and nitrogen relationship of C. zanzibarica inoculated with six rhizobial strains. The greenhouse inoculation experiment revealed that nodule and bacteroid morphologies in C. zanzibarica were rhizobial strain-dependent. Furthermore, C. zanzibarica plants showed significant variation in total plant biomass and nitrogen accumulation among the strains inoculated, while there was very little variation in biomass allocation, nitrogen content, δ15N value and symbiotic efficiency among these tested plants. Results of the greenhouse experiments and field investigations indicated that C. zanzibarica was capable of forming effective symbiosis with diverse rhizobia, which might confer the plant the ability of colonizing various habitats and contribute to its widely distribution in Taiwan. | en |
dc.description.provenance | Made available in DSpace on 2021-05-12T09:34:48Z (GMT). No. of bitstreams: 1 ntu-107-F99b44021-1.pdf: 4653235 bytes, checksum: 8052b9b44f5b5866770e49b53959a35a (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 摘要.................................................................................................................................I
Abstract..........................................................................................................................III Contents….....................................................................................................................VI LIST OF FIGURES…...............................................................................................VIII LIST OF TABLES….....................................................................................................X LIST OF ABBREVIATIONS.......................................................................................XI General introduction…………………...........................................................................1 Chapter 1 C. zanzibarica cultivated in the greenhouse was nodulated by a peculiar strain, CzR2..................................................................................................12 Abstract..................................................................................................................……..13 Introduction....................................................................................................................14 Materials and Methods....................................................................................................16 Results.............................................................................................................................20 Discussion........................................................................................................................23 Figures and Tables...........................................................................................................27 Chapter 2 Rhizobia symbiosis of C. zanzibarica and coexisting legumes growing along Xindian riverbank of Northern Taiwan........................................35 Abstract......................................................................................................................36 Introduction.....................................................................................................................38Materials and Methods....................................................................................................42 Results......................................................................................................................45 Discussion........................................................................................................................48 Figures and Tables............................................................................................56 Chapter 3 Phylogenetic analyses of Bradyrhizobium symbionts associated with invasive C. zanzibarica and its coexisting legumes in Taiwan................64 Abstract.................................................................................................................…….. 65 Introduction.....................................................................................................................66 Materials and Methods....................................................................................................68 Results.............................................................................................................................72 Discussion........................................................................................................................76 Figures and Tables..........................................................................................................83 Chapter 4 Evaluation the growth of C. zanzibarica inoculated by Bradyrhizobium strains..........................................................................................................92 Abstract.................................................................................................................……..93 Introduction....................................................................................................................94 Materials and Methods....................................................................................................95 Results.............................................................................................................................97 Discussion...................................................................................................................99 Figures and Tables.........................................................................................................103 Conclusions...................................................................................................................109 Future works...............................................................................................................111 Literature cited............................................................................................................114 Supplementary materials............................................................................................122 | |
dc.language.iso | en | |
dc.title | 台灣入侵植物南美豬屎豆及其根瘤菌之共生關係 | zh_TW |
dc.title | Symbiotic relationship between an invasive legume, Crotalaria zanzibarica, and its root-nodulating rhizobia in Taiwan | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 博士 | |
dc.contributor.coadvisor | 劉啟德(Chi-Te Liu) | |
dc.contributor.oralexamcommittee | 王俊能(Chun-Neng Wang),湯森林(Sen-Lin Tang),江殷儒(Yin-Ru Chiang),黃政華(Cheng-Hua Huang) | |
dc.subject.keyword | 入侵植物,南美豬屎豆,豆科與根瘤菌共生,慢生型根瘤菌,共生特徵, | zh_TW |
dc.subject.keyword | invasive plant,Crotalaria zanzibarica,legume-rhizobia symbiosis,Bradyrhizobium,symbiotic traits, | en |
dc.relation.page | 136 | |
dc.identifier.doi | 10.6342/NTU201800782 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2018-05-16 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生態學與演化生物學研究所 | zh_TW |
顯示於系所單位: | 生態學與演化生物學研究所 |
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