拉曼影像系統之設計及架設

Abstract

我們在光學顯微鏡上面加裝可以透過液晶調整穿透波長的濾光器和雷射光源，完成了一套拉曼光譜影像系統的架設。可以在75微米的可視範圍之內，透過一次的測量，量到特定波長的拉曼散射在空間上的分佈。半高寬為12公分-1的光譜解析度可以透過矽的拉曼光譜所測得。600奈米的光學解析度則是由聚焦離子束撞擊產生的非晶矽薄膜的邊界所決定。相信有更多關於生物及化學的研究可以透過這一套系統來完成。

We have built a Raman imaging system expanding an optical microscope with a liquid crystal tunable filter (LCTF) and a laser source. Spatial distribution of Raman scattering in a 75um field of view can be measured in one exposure. Spectral resolution has been measured to be 12cm-1 as full width at half maxima (FWHM) on the Raman spectrum of silicon. Spatial resolution has been determined to be 600nm on the edge of ion-beam-induced interfacial amorphization (IBIIA) treated amorphous silicon layer. We are providing an instrument with new possibilities and methods to study biology and chemistry.