利用箭型頭固相微萃取技術開發有機性揮發物分析平台與法醫應用可行性評估

Abstract

揮發性有機分子 (volatile organic compounds, VOC) 是一群具有高蒸氣壓和低水溶性之化合物，充斥於整個環境當中。而人體體內所存在的各種菌相，依據其不同的代謝途徑，會產生各式各樣的 VOC，在過去已陸續針對人體所呼出 VOC 以及人體死後所產生的 VOC 進行研究分析。本研究使用近年來新發展出的箭型頭固相微萃取法 (SPME arrow) 進行實驗及開發分析平台。在建立 VOC 的萃取方法後，實際應用在法醫層面，分析不同環境或不同時間作用下，其微生物相所產生的 VOC 亦不盡相同，亦針對同個環境下比較不同器官所產生 VOC 的差異性，建立其所產生的 VOC 種類，並討論可能影響的變因。此外使用人體組織進行實驗，試圖找出潛在 VOC 用於對死後時間間隔的研判。然而實際法醫檢體有取得上的困難及相關資訊的不足，以及影響死後變化的變因過多，尚未能有效找出合適於判斷死後時間間隔之 VOC。

Volatile organic compounds (VOCs) are a group of compounds with high vapor pressure and low water solubility, prevalent in the environment. Various microbial communities within the human body produce diverse VOCs through different metabolic pathways. Previous studies have systematically analyzed VOCs exhaled by humans and those produced post-mortem. This study employs the recently developed solid-phase microextraction arrow (SPME arrow) method to experimentally develop an analysis platform. After establishing methods for VOC extraction, practical applications in forensic science involve analyzing VOCs produced by microbial communities under different environmental conditions and over time. The study also compares VOCs produced by different organs within the same environment, categorizing their types and discussing influencing factors. Additionally, experiments using human tissues aim to identify potential VOCs for estimating post-mortem intervals. Challenges in obtaining forensic samples and insufficient related information, coupled with numerous variables affecting post-mortem changes, have hindered the effective identification of VOCs suitable for determining post-mortem intervals.