樂高層光顯微鏡研製於試件微變形量測與分析之應用

Abstract

數位影像關聯法（Digital Image Correlation, DIC）為一種非接觸式的光學量測技術，可應用於跨尺度與跨領域的工程問題以及實驗力學研究上，其原理為透過數位影像分析，追蹤樣本影像表面特徵產生的變化，來取得樣本的位移場與應變場等物理量；於先進光學技術方面，層光顯微鏡具備高訊躁比、快速的影像採樣速率以及較低的生物光毒性與光損傷效應等特點，使其被廣泛運用在生物醫學領域，小至單細胞到大型生物器官都能夠進行觀測。本論文首先將介紹樂高層光顯微鏡的系統設計以及搭建步驟，並在樂高層光顯微鏡之載物台上將樣本進行平移與旋轉以及引致變形的操作，再以數位影像關聯法量測樣本的位移場，並將量測位移量與理論值對照是否相符，以此方法來驗證樂高層光顯微鏡系統的穩定度。 論文內容：第一章說明了本論文的研究動機與背景，並簡要介紹本研究開發的技術和相關應用的方法；第二章將介紹層光顯微技術的發展歷史以及層光顯微鏡運作的原理，並描述層光顯微鏡的特點，以及其適合觀察生物樣本的優勢；第三章將介紹數位影像關聯法數學理論，並說明如何在MATLAB中操作數位影像關聯法開源軟體NCORR；第四章為以樂高積木搭建層光顯微鏡的詳細說明，也會一併介紹顯微鏡各項元件與控制系統，並展示以樂高層光顯微鏡觀察樂高積木樣本的成果；第五章為以數位影像關聯法分析光學量測影像的結果；第六章為本論文結論與未來展望。

Digital Image Correlation (DIC) is a non-contact optical measurement technique that can be applied to cross-scale and cross-domain engineering problems as well as experimental mechanics research. The principle of DIC is to track the changes in the surface features of a sample through digital image analysis to obtain the physical quantities such as displacement field and strain field of the sample. Light sheet microscopy, characterized by high signal-to-noise ratio, rapid image sampling rates, and lower biological phototoxicity and photodamage effects, is widely used in the biomedical field, and enabling observation ranging from single cells to large biological organs. In this paper, we will firstly introduce the system and the construction steps of the LEGO light sheet microscope, then observe the sample on the microscope’s stage through translation, rotation, and deformation, and then measure whether the displacement field of the samples matches with the actual displacement by digital image correlation method, so that the stability of the LEGO light sheet microscope can be verified. Paper content: Chapter 1 provides a literature review on the development and background of the digital image correlation method and light sheet microscopy. Chapter 2 introduces the development history of light sheet microscopy technology, and the principle of operation of light sheet microscope, then compares the advantages and disadvantages of layered light microscopy. Also show the reasons why it is suitable for observing biological samples. Chapter 3 introduces the theory of digital image correlation and explains how to operate NCORR for digital image correlation in MATLAB. In Chapter 4, we will detail how to build a simple light sheet microscope using LEGO bricks, as well as introduce the microscope's control system and experimental procedures of digital image correlation measurements. Chapter 5 presents the measurement results of digital image correlation and compares them with experimental data for discussion. Chapter 6 concludes the paper and provides future prospects.