請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/92077
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 呂廷璋 | zh_TW |
dc.contributor.advisor | Ting-Jang Lu | en |
dc.contributor.author | 林稚傑 | zh_TW |
dc.contributor.author | Chih-Chieh Lin | en |
dc.date.accessioned | 2024-03-04T16:24:37Z | - |
dc.date.available | 2024-03-14 | - |
dc.date.copyright | 2024-03-04 | - |
dc.date.issued | 2024 | - |
dc.date.submitted | 2024-02-14 | - |
dc.identifier.citation | 行政院衛生福利部食品藥物管理署,食品化學檢驗方法之確效規範。2011 年 12 月 1 日。行政院衛生福利部食品藥物管理署,台灣。
呂振宇,以高效能液相層析串聯質譜儀分析半乳寡醣之結構特徵。國立臺灣大學食品科技研究所學位論文 2014。 楊硯喬,以高效能液相層析串聯質譜法分析半乳寡醣樣品之組成結構。國立臺灣大學食品科技研究所學位論文 2016。 經濟部標準檢驗局,中華民國國家標準 CNS 1305 N 5024。2012。經濟部標準檢驗局,台灣。 Al-Sheraji, S. H.; Ismail, A.; Manap, M. Y.; Mustafa, S.; Yusof, R. M.; Hassan, F. A., Prebiotics as functional foods: A review. Journal of Functional Foods 2013, 5, 1542-1553. Arias, V. C.; Castells, R. C.; Malacalza, N.; Lupano, C. E.; Castells, C. B., Determination of Oligosaccharide Patterns in Honey by Solid-Phase Extraction and High-Performance Liquid Chromatography. Chromatographia 2003, 58, 797-801. Balto, A. S.; Lapis, T. J.; Silver, R. K.; Ferreira, A. J.; Beaudry, C. M.; Lim, J.; Penner, M. H., On the use of differential solubility in aqueous ethanol solutions to narrow the DP range of food-grade starch hydrolysis products. Food chemistry 2016, 197, 872-880. Banti, M., Raffinose Family Oligosaccharides, Occurrence in Food Materials, Nutritional Implication and Methods of Analysis, a Review. World Journal of Food Science and Technology 2021, 5, 37-44. Bao, Y.; Chen, C.; Newburg, D. S., Quantification of neutral human milk oligosaccharides by graphitic carbon high-performance liquid chromatography with tandem mass spectrometry. Analytical Biochemistry 2013, 433, 28-35. Benkeblia, N., Fructooligosaccharides and fructans analysis in plants and food crops. Journal of Chromatography A 2013, 1313, 54-61. Bode, L., Human milk oligosaccharides: Every baby needs a sugar mama. Glycobiology 2012, 22, 1147-1162. Bode, L.; Jantscher-Krenn, E., Structure-Function Relationships of Human Milk Oligosaccharides. Advances in Nutrition 2012, 3, 383S-391S. Borewicz, K.; Gu, F.; Saccenti, E.; Arts, I. C. W.; Penders, J.; Thijs, C.; van Leeuwen, S. S.; Lindner, C.; Nauta, A.; van Leusen, E.; Schols, H. A.; Smidt, H., Correlating Infant Fecal Microbiota Composition and Human Milk Oligosaccharide Consumption by Microbiota of 1-Month-Old Breastfed Infants. Molecular Nutrition & Food Research 2019, 63, 1801214. Brzozowski, A. M.; Davies, G. J., Structure of the Aspergillus oryzae α-Amylase Complexed with the Inhibitor Acarbose at 2.0 Å Resolution. Biochemistry 1997, 36, 10837-10845. Córdova, A.; Astudillo, C.; Giorno, L.; Guerrero, C.; Conidi, C.; Illanes, A.; Cassano, A., Nanofiltration potential for the purification of highly concentrated enzymatically produced oligosaccharides. Food and Bioproducts Processing 2016, 98, 50-61. Cao, L.; Tolić, N.; Qu, Y.; Meng, D.; Zhao, R.; Zhang, Q.; Moore, R. J.; Zink, E. M.; Lipton, M. S.; Paša-Tolić, L.; Wu, S., Characterization of intact N- and O-linked glycopeptides using higher energy collisional dissociation. Analytical Biochemistry 2014, 452, 96-102. Cardelle-Cobas, A.; Corzo, N.; Olano, A.; Peláez, C.; Requena, T.; Ávila, M., Galactooligosaccharides derived from lactose and lactulose: Influence of structure on Lactobacillus, Streptococcus and Bifidobacterium growth. International Journal of Food Microbiology 2011, 149, 81-87. Carević, M.; Bezbradica, D.; Banjanac, K.; Milivojević, A.; Fanuel, M.; Rogniaux, H.; Ropartz, D.; Veličković, D., Structural Elucidation of Enzymatically Synthesized Galacto-oligosaccharides Using Ion-Mobility Spectrometry–Tandem Mass Spectrometry. Journal of Agricultural and Food Chemistry 2016, 64, 3609-3615. Casa-Villegas, M.; Marín-Navarro, J.; Polaina, J., Amylases and related glycoside hydrolases with transglycosylation activity used for the production of isomaltooligosaccharides. Amylase 2018, 2, 17-29. Catenza, K. F.; Donkor, K. K., Recent approaches for the quantitative analysis of functional oligosaccharides used in the food industry: A review. Food Chemistry 2021, 355, 129416. Chen, Y.; Liu, Y., Characterization of galacto-oligosaccharides using high-performance anion exchange chromatography-tandem mass spectrometry. Journal of Separation Science 2021, 44, 2221-2233. Chong, J.; Wishart, D. S.; Xia, J., Using MetaboAnalyst 4.0 for Comprehensive and Integrative Metabolomics Data Analysis. Current Protocols in Bioinformatics 2019, 68, e86. Coppa, G. V.; Pierani, P.; Zampini, L.; Carloni, I.; Carlucci, A.; Gabrielli, O., Oligosaccharides in human milk during different phases of lactation. Acta Paediatrica 1999, 88, 89-94. Costello, C. E.; Contado-Miller, J. M.; Cipollo, J. F., A glycomics platform for the analysis of permethylated oligosaccharide alditols. Journal of the American Society for Mass Spectrometry 2007, 18, 1799-1812. Cotte, J.-F.; Casabianca, H.; Chardon, S.; Lheritier, J.; Grenier-Loustalot, M.-F., Application of carbohydrate analysis to verify honey authenticity. Journal of Chromatography A 2003, 1021, 145-155. Coulier, L.; Timmermans, J.; Bas, R.; Van Den Dool, R.; Haaksman, I.; Klarenbeek, B.; Slaghek, T.; Van Dongen, W., In-Depth Characterization of Prebiotic Galacto-oligosaccharides by a Combination of Analytical Techniques. Journal of Agricultural and Food Chemistry 2009, 57, 8488-8495. Cummings, J. H.; Stephen, A. M., Carbohydrate terminology and classification. European Journal of Clinical Nutrition 2007, 61, S5-S18. Da Costa Leite, J. M.; Trugo, L. C.; Costa, L. S. M.; Quinteiro, L. M. C.; Barth, O. M.; Dutra, V. M. L.; De Maria, C. A. B., Determination of oligosaccharides in Brazilian honeys of different botanical origin. Food Chemistry 2000, 70, 93-98. Depeint, F.; Tzortzis, G.; Vulevic, J.; I'Anson, K.; Gibson, G. R., Prebiotic evaluation of a novel galactooligosaccharide mixture produced by the enzymatic activity of Bifidobacterium bifidum NCIMB 41171, in healthy humans: a randomized, double-blind, crossover, placebo-controlled intervention study. The American Journal of Clinical Nutrition 2008, 87, 785-791. Difilippo, E.; Pan, F.; Logtenberg, M.; Willems, R.; Braber, S.; Fink-Gremmels, J.; Schols, H. A.; Gruppen, H., In Vitro Fermentation of Porcine Milk Oligosaccharides and Galacto-oligosaccharides Using Piglet Fecal Inoculum. Journal of Agricultural and Food Chemistry 2016, 64, 2127-2133. Domon, B.; Costello, C. E., A systematic nomenclature for carbohydrate fragmentations in FAB-MS/MS spectra of glycoconjugates. Glycoconjugate journal 1988, 5, 397-409. Dumté, M. E. J.; Manley-Harris, M., Oligosaccharide profiles of Asian commercial honeys. Chemistry in New Zealand 2012, 76, 80-84. EU Directive, Council Directive 2001/110/EC of 20 December 2001 relating to honey. In Official Journal of the European Communities, 2001; Vol. 45, pp 47-52. Flamm, G.; Glinsmann, W.; Kritchevsky, D.; Prosky, L.; Roberfroid, M., Inulin and Oligofructose as Dietary Fiber: A Review of the Evidence. Critical Reviews in Food Science and Nutrition 2001, 41, 353-362. Fransen, C. T. M.; Van Laere, K. M. J.; van Wijk, A. A. C.; Brüll, L. P.; Dignum, M.; Thomas-Oates, J. E.; Haverkamp, J.; Schols, H. A.; Voragen, A. G. J.; Kamerling, J. P.; Vliegenthart, J. F. G., α-d-Glcp-(1↔1)-β-d-Galp-containing oligosaccharides, novel products from lactose by the action of β-galactosidase. Carbohydrate Research 1998, 314, 101-114. Gómez Bárez, J. A.; Garcia-Villanova, R. J.; Elvira Garcia, S.; González Paramás, A. M., Optimization of the capillary gas chromatographic analysis of mono- and oligosaccharides in honeys. Chromatographia 1999, 50, 461-469. Gangola, M. P.; Jaiswal, S.; Khedikar, Y. P.; Chibbar, R. N., A reliable and rapid method for soluble sugars and RFO analysis in chickpea using HPAEC–PAD and its comparison with HPLC–RI. Food Chemistry 2014, 154, 127-133. Garozzo, D.; Giuffrida, M.; Impallomeni, G.; Ballistreri, A.; Montaudo, G., Determination of linkage position and identification of the reducing end in linear oligosaccharides by negative ion fast atom bombardment mass spectrometry. Analytical chemistry 1990, 62, 279-286. Garozzo, D.; Impallomeni, G.; Spina, E.; Green, B. N.; Hutton, T., Linkage analysis in disaccharides by electrospray mass spectrometry. Carbohydrate research 1991, 221, 253-257. Gašić, U.; Kečkeš, S.; Dabić, D.; Trifković, J.; Milojković-Opsenica, D.; Natić, M.; Tešić, Ž., Phenolic profile and antioxidant activity of Serbian polyfloral honeys. Food Chemistry 2014, 145, 599-607. Goffin, D.; Delzenne, N.; Blecker, C.; Hanon, E.; Deroanne, C.; Paquot, M., Will Isomalto-Oligosaccharides, a Well-Established Functional Food in Asia, Break through the European and American Market? The Status of Knowledge on these Prebiotics. Critical Reviews in Food Science and Nutrition 2011, 51, 394-409. Goffin, D.; Wathelet, B.; Blecker, C.; Deroanne, C.; Malmendier, Y.; Paquot, M., Comparison of the glucooligosaccharide profiles produced from maltose by two different transglucosidases from Aspergillus niger. Biotechnology, Agronomy, Society and Environment 2010, 14, 607-616. Gänzle, M. G., Lactose and Oligosaccharides | Lactose: Galacto-Oligosaccharides. In Encyclopedia of Dairy Sciences (Second Edition), Fuquay, J. W., Ed. Academic Press: San Diego, 2011; pp 209-216. Gosling, A.; Stevens, G. W.; Barber, A. R.; Kentish, S. E.; Gras, S. L., Recent advances refining galactooligosaccharide production from lactose. Food Chemistry 2010, 121, 307-318. Guo, Q.; Goff, H. D.; Cui, S. W., Structural characterisation of galacto-oligosaccharides (VITAGOS™) sythesized by transgalactosylation of lactose. Bioactive Carbohydrates and Dietary Fibre 2018, 14, 33-38. Hao, Q.; Nan, T.; Zhou, L.; Kang, L.; Guo, L.; Yu, Y., Rapid simultaneous quantification of fructooligosaccharides in Morinda officianalis by ultra-high performance liquid chromatography. Journal of Separation Science 2019, 42, 2222-2230. Harazono, A.; Kobayashi, T.; Kawasaki, N.; Itoh, S.; Tada, M.; Hashii, N.; Ishii, A.; Arato, T.; Yanagihara, S.; Yagi, Y.; Koga, A.; Tsuda, Y.; Kimura, M.; Sakita, M.; Kitamura, S.; Yamaguchi, H.; Mimura, H.; Murata, Y.; Hamazume, Y.; Sato, T.; Natsuka, S.; Kakehi, K.; Kinoshita, M.; Watanabe, S.; Yamaguchi, T., A comparative study of monosaccharide composition analysis as a carbohydrate test for biopharmaceuticals. Biologicals 2011, 39, 171-180. He, Y.; Liu, S.; Kling, D. E.; Leone, S.; Lawlor, N. T.; Huang, Y.; Feinberg, S. B.; Hill, D. R.; Newburg, D. S., The human milk oligosaccharide 2′-fucosyllactose modulates CD14 expression in human enterocytes, thereby attenuating LPS-induced inflammation. Gut 2016, 65, 33-46. Hernández-Hernández, O.; Calvillo, I.; Lebrón-Aguilar, R.; Moreno, F. J.; Sanz, M. L., Hydrophilic interaction liquid chromatography coupled to mass spectrometry for the characterization of prebiotic galactooligosaccharides. Journal of Chromatography A 2012, 1220, 57-67. Hernández, O.; Ruiz-Matute, A. I.; Olano, A.; Moreno, F. J.; Sanz, M. L., Comparison of fractionation techniques to obtain prebiotic galactooligosaccharides. International Dairy Journal 2009, 19, 531-536. Hong, Q.; Ruhaak, L. R.; Totten, S. M.; Smilowitz, J. T.; German, J. B.; Lebrilla, C. B., Label-Free Absolute Quantitation of Oligosaccharides Using Multiple Reaction Monitoring. Analytical Chemistry 2014, 86, 2640-2647. Hsu, H. C.; Huang, S.-P.; Liew, C. Y.; Tsai, S.-T.; Ni, C.-K., De novo structural determination of mannose oligosaccharides by using a logically derived sequence for tandem mass spectrometry. Analytical and Bioanalytical Chemistry 2019, 411, 3241-3255. Hsu, H. C.; Liew, C. Y.; Huang, S.-P.; Tsai, S.-T.; Ni, C.-K., Simple Approach for De Novo Structural Identification of Mannose Trisaccharides. Journal of The American Society for Mass Spectrometry 2018a, 29, 470-480. Hsu, H. C.; Liew, C. Y.; Huang, S.-P.; Tsai, S.-T.; Ni, C.-K., Simple Method for De Novo Structural Determination of Underivatised Glucose Oligosaccharides. Scientific Reports 2018b, 8, 5562. Hu, X.; Liu, C.; Jin, Z.; Tian, Y., Fractionation of starch hydrolysate into dextrin fractions with low dispersity by gradient alcohol precipitation. Separation and Purification Technology 2015, 151, 201-210. Jantscher-Krenn, E.; Aigner, J.; Reiter, B.; Köfeler, H.; Csapo, B.; Desoye, G.; Bode, L.; van Poppel, M. N. M., Evidence of human milk oligosaccharides in maternal circulation already during pregnancy: a pilot study. American Journal of Physiology-Endocrinology and Metabolism 2018, 316, E347-E357. Jedrychowski, M. P.; Huttlin, E. L.; Haas, W.; Sowa, M. E.; Rad, R.; Gygi, S. P., Evaluation of HCD- and CID-type fragmentation within their respective detection platforms for murine phosphoproteomics. Mol Cell Proteomics 2011, 10, M111.009910. Jiao, Y.; Li, F.; Jiang, D.; Li, W.; Chen, J., Determination of Isomaltooligosaccharides in Milk Powder by Ultra-High Performance Liquid Chromatography–Tandem Mass Spectrometry. Analytical Letters 2016, 49, 458-466. Jorge, T. F.; Florêncio, M. H.; António, C., Porous Graphitic Carbon Liquid Chromatography–Mass Spectrometry Analysis of Drought Stress-Responsive Raffinose Family Oligosaccharides in Plant Tissues. In Plant Stress Tolerance: Methods and Protocols, Sunkar, R., Ed. Springer New York: New York, NY, 2017; pp 279-293. Juers, D. H.; Heightman, T. D.; Vasella, A.; McCarter, J. D.; Mackenzie, L.; Withers, S. G.; Matthews, B. W., A Structural View of the Action of Escherichia coli (lacZ) β-Galactosidase. Biochemistry 2001, 40, 14781-14794. Kaneko, T.; Kohmoto, T.; Kikuchi, H.; Shiota, M.; Iino, H.; Mitsuoka, T., Effects of Isomaltooligosaccharides with Different Degrees of Polymerization on Human Fecal Bifidobacteria. Bioscience, Biotechnology, and Biochemistry 1994, 58, 2288-2290. Kaškonienė, V.; Venskutonis, P. R., Floral Markers in Honey of Various Botanical and Geographic Origins: A Review. Comprehensive Reviews in Food Science and Food Safety 2010, 9, 620-634. Kečkeš, S.; Gašić, U.; Veličković, T. Ć.; Milojković-Opsenica, D.; Natić, M.; Tešić, Ž., The determination of phenolic profiles of Serbian unifloral honeys using ultra-high-performance liquid chromatography/high resolution accurate mass spectrometry. Food Chemistry 2013, 138, 32-40. Ko, J.; Lee, M.-S.; Kwak, B.-M.; Ahn, J.-H.; Park, J.-S.; Kwon, J.-H., Determination of isomaltooligosaccharides in yoghurts by using HPLC-ELSD. Food Science of Animal Resources 2013, 33, 417-424. Korošec, M.; Bertoncelj, J.; Pereyra Gonzales, A.; Kropf, U.; Golob, U.; Golob, T. J. A. a., Monosaccharides and oligosaccharides in four types of Slovenian honey. Acta Alimentaria 2009, 38, 459-469. Kuhn, R. C.; Mazutti, M. A.; Albertini, L. B.; Maugeri Filho, F., Evaluation of fructooligosaccharides separation using a fixed-bed column packed with activated charcoal. New biotechnology 2014, 31, 237-241. Kumar, S., Chapter 21 - Developments, advancements, and contributions of mass spectrometry in omics technologies. In Advances in Protein Molecular and Structural Biology Methods, Tripathi, T.; Dubey, V. K., Eds. Academic Press: 2022; pp 327-356. Kumar, V.; Rani, A.; Goyal, L.; Dixit, A. K.; Manjaya, J. G.; Dev, J.; Swamy, M., Sucrose and Raffinose Family Oligosaccharides (RFOs) in Soybean Seeds As Influenced by Genotype and Growing Location. Journal of Agricultural and Food Chemistry 2010, 58, 5081-5085. Kuo, C.-W.; Khoo, K.-H., Strategic Applications of Negative-Mode LC-MS/MS Analyses to Expedite Confident Mass Spectrometry-Based Identification of Multiple Glycosylated Peptides. Analytical Chemistry 2020, 92, 7612-7620. Ladirat, S. E.; Schols, H. A.; Nauta, A.; Schoterman, M. H. C.; Schuren, F. H. J.; Gruppen, H., In vitro fermentation of galacto-oligosaccharides and its specific size-fractions using non-treated and amoxicillin-treated human inoculum. Bioactive Carbohydrates and Dietary Fibre 2014, 3, 59-70. Li, W.; Wang, K.; Sun, Y.; Ye, H.; Hu, B.; Zeng, X., Influences of structures of galactooligosaccharides and fructooligosaccharides on the fermentation in vitro by human intestinal microbiota. Journal of Functional Foods 2015, 13, 158-168. Li, Y.; Jin, Y.; Yang, S.; Zhang, W.; Zhang, J.; Zhao, W.; Chen, L.; Wen, Y.; Zhang, Y.; Lu, K.; Zhang, Y.; Zhou, J.; Yang, S., Strategy for comparative untargeted metabolomics reveals honey markers of different floral and geographic origins using ultrahigh-performance liquid chromatography-hybrid quadrupole-orbitrap mass spectrometry. Journal of Chromatography A 2017, 1499, 78-89. Liang, T.; Fu, Q.; Li, F.; Zhou, W.; Xin, H.; Wang, H.; Jin, Y.; Liang, X., Hydrophilic interaction liquid chromatography for the separation, purification, and quantification of raffinose family oligosaccharides from Lycopus lucidus Turcz. Journal of Separation Science 2015, 38, 2607-2613. Lin, A. E.; Autran, C. A.; Szyszka, A.; Escajadillo, T.; Huang, M.; Godula, K.; Prudden, A. R.; Boons, G.-J.; Lewis, A. L.; Doran, K. S.; Nizet, V.; Bode, L., Human milk oligosaccharides inhibit growth of group B Streptococcus. Journal of Biological Chemistry 2017, 292, 11243-11249. Liu, Z.; Moate, P.; Cocks, B.; Rochfort, S., Simple Liquid Chromatography–Mass Spectrometry Method for Quantification of Major Free Oligosaccharides in Bovine Milk. Journal of Agricultural and Food Chemistry 2014, 62, 11568-11574. Logtenberg, M. J.; Akkerman, R.; Hobé, R. G.; Donners, K. M. H.; Van Leeuwen, S. S.; Hermes, G. D. A.; de Haan, B. J.; Faas, M. M.; Buwalda, P. L.; Zoetendal, E. G.; de Vos, P.; Schols, H. A., Structure-Specific Fermentation of Galacto-Oligosaccharides, Isomalto-Oligosaccharides and Isomalto/Malto-Polysaccharides by Infant Fecal Microbiota and Impact on Dendritic Cell Cytokine Responses. 2021, 65, 2001077. Logtenberg, M. J.; Donners, K. M. H.; Vink, J. C. M.; van Leeuwen, S. S.; de Waard, P.; de Vos, P.; Schols, H. A., Touching the High Complexity of Prebiotic Vivinal Galacto-oligosaccharides Using Porous Graphitic Carbon Ultra-High-Performance Liquid Chromatography Coupled to Mass Spectrometry. Journal of Agricultural and Food Chemistry 2020, 68, 7800-7808. Low, N. H.; Nelson, D. L.; Sporns, P., Carbohydrate Analysis of Western Canadian Honeys and their Nectar Sources to Determine the Origin of Honey Oligosaccharides. Journal of Apicultural Research 1988, 27, 245-251. Madsen Ii, L. R.; Stanley, S.; Swann, P.; Oswald, J., A Survey of Commercially Available Isomaltooligosaccharide-Based Food Ingredients. Journal of Food Science 2017, 82, 401-408. Maina, N. H.; Juvonen, M.; Domingues, R. M.; Virkki, L.; Jokela, J.; Tenkanen, M., Structural analysis of linear mixed-linkage glucooligosaccharides by tandem mass spectrometry. Food chemistry 2013, 136, 1496-1507. Mank, M.; Welsch, P.; Heck, A. J. R.; Stahl, B., Label-free targeted LC-ESI-MS2 analysis of human milk oligosaccharides (HMOS) and related human milk groups with enhanced structural selectivity. Analytical and Bioanalytical Chemistry 2019, 411, 231-250. Marianou, A. A.; Michailof, C. M.; Pineda, A.; Iliopoulou, E. F.; Triantafyllidis, K. S.; Lappas, A. A., Glucose to Fructose Isomerization in Aqueous Media over Homogeneous and Heterogeneous Catalysts. 2016, 8, 1100-1110. Matsuzawa, T.; Jo, T.; Uchiyama, T.; Manninen, J. A.; Arakawa, T.; Miyazaki, K.; Fushinobu, S.; Yaoi, K., Crystal structure and identification of a key amino acid for glucose tolerance, substrate specificity, and transglycosylation activity of metagenomic β-glucosidase Td2F2. The FEBS Journal 2016, 283, 2340-2353. Michelon, M.; Manera, A. P.; Carvalho, A. L.; Maugeri Filho, F., Concentration and purification of galacto‐oligosaccharides using nanofiltration membranes. International journal of food science & technology 2014, 49, 1953-1961. Moreno, F. J.; Corzo, N.; Montilla, A.; Villamiel, M.; Olano, A., Current state and latest advances in the concept, production and functionality of prebiotic oligosaccharides. Current Opinion in Food Science 2017, 13, 50-55. Mussatto, S. I.; Mancilha, I. M., Non-digestible oligosaccharides: A review. Carbohydrate Polymers 2007, 68, 587-597. Ota, M.; Okamoto, T.; Hoshino, W.; Wakabayashi, H., Action of α-d-glucosidase from Aspergillus niger towards dextrin and starch. Carbohydrate Polymers 2009a, 78, 287-291. Ota, M.; Okamoto, T.; Wakabayashi, H., Action of transglucosidase from Aspergillus niger on maltoheptaose and [U–13C]maltose. Carbohydrate Research 2009b, 344, 460-465. Ouchemoukh, S.; Schweitzer, P.; Bachir Bey, M.; Djoudad-Kadji, H.; Louaileche, H., HPLC sugar profiles of Algerian honeys. Food Chemistry 2010, 121, 561-568. Pöhnl, T.; Böttcher, C.; Schulz, H.; Stürtz, M.; Widder, S.; Carle, R.; Schweiggert, R. M., Comparison of high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and ultra-high performance liquid chromatography with evaporative light scattering (UHPLC-ELSD) for the analyses of fructooligosaccharides in onion (Allium cepa L.). Journal of Food Composition and Analysis 2017, 63, 148-156. Packer, N. H.; Lawson, M. A.; Jardine, D. R.; Redmond, J. W., A general approach to desalting oligosaccharides released from glycoproteins. Glycoconjugate Journal 1998, 15, 737-747. Phengnoi, P.; Charoenwongpaiboon, T.; Wangpaiboon, K.; Klaewkla, M.; Nakapong, S.; Visessanguan, W.; Ito, K.; Pichyangkura, R.; Kuttiyawong, K., Levansucrase from Bacillus amyloliquefaciens KK9 and Its Y237S Variant Producing the High Bioactive Levan-Type Fructooligosaccharides. Biomolecules 2020, 10. Pisanello, D.; Caruso, G., EU Regulation on Novel Foods. In Novel Foods in the European Union, Pisanello, D.; Caruso, G., Eds. Springer International Publishing: Cham, 2018; pp 1-29. Prieto-Santiago, V.; Cavia, M. d. M.; Barba, F. J.; Alonso-Torre, S. R.; Carrillo, C., Multiple reaction monitoring for identification and quantification of oligosaccharides in legumes using a triple quadrupole mass spectrometer. Food Chemistry 2022, 368, 130761. Pruksasri, S.; Nguyen, T.-H.; Haltrich, D.; Novalin, S., Fractionation of a galacto-oligosaccharides solution at low and high temperature using nanofiltration. Separation and purification technology 2015, 151, 124-130. Raga-Carbajal, E.; López-Munguía, A.; Alvarez, L.; Olvera, C., Understanding the transfer reaction network behind the non-processive synthesis of low molecular weight levan catalyzed by Bacillus subtilis levansucrase. Scientific Reports 2018, 8, 15035. Reinhold, V. N.; Sheeley, D. M., Detailed characterization of carbohydrate linkage and sequence in an ion trap mass spectrometer: glycosphingolipids. Analytical Biochemistry 1998, 259, 28-33. Remoroza, C. A.; Mak, T. D.; De Leoz, M. L. A.; Mirokhin, Y. A.; Stein, S. E., Creating a Mass Spectral Reference Library for Oligosaccharides in Human Milk. Analytical Chemistry 2018, 90, 8977-8988. Roberfroid, M.; Slavin, J., Nondigestible Oligosaccharides. Critical Reviews in Food Science and Nutrition 2000, 40, 461-480. Ruhaak, L. R.; Deelder, A. M.; Wuhrer, M., Oligosaccharide analysis by graphitized carbon liquid chromatography–mass spectrometry. Analytical and Bioanalytical Chemistry 2009, 394, 163-174. Ruhaak, L. R.; Lebrilla, C. B., Advances in Analysis of Human Milk Oligosaccharides. Advances in Nutrition 2012, 3, 406S-414S. Ruhaak, L. R.; Zauner, G.; Huhn, C.; Bruggink, C.; Deelder, A. M.; Wuhrer, M., Glycan labeling strategies and their use in identification and quantification. Analytical and Bioanalytical Chemistry 2010, 397, 3457-3481. Sabater, C.; Prodanov, M.; Olano, A.; Corzo, N.; Montilla, A., Quantification of prebiotics in commercial infant formulas. Food Chemistry 2016, 194, 6-11. Salonen, A.; Hiltunen, J.; Julkunen-Tiitto, R., Composition of unique unifloral honeys from the boreal coniferous forest zone: Fireweed and raspberry honey. Journal of ApiProduct ApiMedical Science 2011, 3, 128-136. Santos-Moriano, P.; Fernandez-Arrojo, L.; Poveda, A.; Jimenez-Barbero, J.; Ballesteros, A. O.; Plou, F. J., Levan versus fructooligosaccharide synthesis using the levansucrase from Zymomonas mobilis: Effect of reaction conditions. Journal of Molecular Catalysis B: Enzymatic 2015, 119, 18-25. Sen, D.; Gosling, A.; Stevens, G. W.; Bhattacharya, P. K.; Barber, A. R.; Kentish, S. E.; Bhattacharjee, C.; Gras, S. L., Galactosyl oligosaccharide purification by ethanol precipitation. Food Chemistry 2011, 128, 773-777. Sheeley, D. M.; Reinhold, V. N., Structural characterization of carbohydrate sequence, linkage, and branching in a quadrupole Ion trap mass spectrometer: neutral oligosaccharides and N-linked glycans. Analytical Chemistry 1998, 70, 3053-3059. Simões, J.; Domingues, P.; Reis, A.; Nunes, F. M.; Coimbra, M. A.; Domingues, M. R. M., Identification of anomeric configuration of underivatized reducing glucopyranosyl-glucose disaccharides by tandem mass spectrometry and multivariate analysis. Analytical chemistry 2007, 79, 5896-5905. Singh, R. S.; Singh, R. P., Production of fructooligosaccharides from inulin by endoinulinases and their prebiotic potential. Food Technology and Biotechnology 2010, 48, 435. Skoog, D. A.; West, D. M.; Holler, F. J.; Crouch, S. R., Fundamentals of analytical chemistry. Nelson Education: 2013. Slegte, J. d., Determination of trans-Galactooligosaccharides in Selected Food Products by Ion-Exchange Chromatography: Collaborative Study. Journal of AOAC International 2002, 85, 417-423. Sorndech, W.; Nakorn, K. N.; Tongta, S.; Blennow, A., Isomalto-oligosaccharides: Recent insights in production technology and their use for food and medical applications. LWT 2018, 95, 135-142. Swallow, K. W.; Low, N. H., Analysis and quantitation of the carbohydrates in honey using high-performance liquid chromatography. Journal of Agricultural and Food Chemistry 1990, 38, 1828-1832. Tao, N.; DePeters, E. J.; Freeman, S.; German, J. B.; Grimm, R.; Lebrilla, C. B., Bovine Milk Glycome. Journal of Dairy Science 2008, 91, 3768-3778. Thurl, S.; Munzert, M.; Boehm, G.; Matthews, C.; Stahl, B., Systematic review of the concentrations of oligosaccharides in human milk. Nutrition Reviews 2017, 75, 920-933. Tokuoka, M.; Honda, C.; Totsuka, A.; Shindo, H.; Hosaka, M., Analysis of the oligosaccharides in Japanese rice wine, sake, by hydrophilic interaction liquid chromatography–time-of-flight/mass spectrometry. Journal of Bioscience and Bioengineering 2017, 124, 171-177. Tonon, K. M.; Miranda, A.; Abrão, A. C. F. V.; de Morais, M. B.; Morais, T. B., Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography – electrospray ionization – mass spectrometry. Food Chemistry 2019, 274, 691-697. Torres, D. P.; Gonçalves, M. d. P. F.; Teixeira, J. A.; Rodrigues, L. R., Galacto‐oligosaccharides: Production, properties, applications, and significance as prebiotics. Comprehensive Reviews in Food Science and Food Safety 2010, 9, 438-454. Triantis, V.; Bode, L.; van Neerven, R. J. J., Immunological Effects of Human Milk Oligosaccharides. 2018, 6. Tsai, S.-T.; Chen, J.-L.; Ni, C.-K., Does low-energy collision-induced dissociation of lithiated and sodiated carbohydrates always occur at anomeric carbon of the reducing end? 2017, 31, 1835-1844. Tungland, B. C.; Meyer, D., Nondigestible Oligo- and Polysaccharides (Dietary Fiber): Their Physiology and Role in Human Health and Food. Comprehensive Reviews In Food Science And Food Safety 2002, 1, 90-109. Uchiyama, T.; Miyazaki, K.; Yaoi, K., Characterization of a Novel β-Glucosidase from a Compost Microbial Metagenome with Strong Transglycosylation Activity *. Journal of Biological Chemistry 2013, 288, 18325-18334. Urrutia, P.; Rodriguez-Colinas, B.; Fernandez-Arrojo, L.; Ballesteros, A. O.; Wilson, L.; Illanes, A.; Plou, F. J., Detailed Analysis of Galactooligosaccharides Synthesis with β-Galactosidase from Aspergillus oryzae. Journal of Agricultural and Food Chemistry 2013, 61, 1081-1087. van Leeuwen, S. S., Challenges and Pitfalls in Human Milk Oligosaccharide Analysis. Nutrients 2019, 11. van Leeuwen, S. S.; Kuipers, B. J. H.; Dijkhuizen, L.; Kamerling, J. P., 1H NMR analysis of the lactose/β-galactosidase-derived galacto-oligosaccharide components of Vivinal® GOS up to DP5. Carbohydrate Research 2014a, 400, 59-73. van Leeuwen, S. S.; Kuipers, B. J. H.; Dijkhuizen, L.; Kamerling, J. P., Development of a 1H NMR structural-reporter-group concept for the analysis of prebiotic galacto-oligosaccharides of the [β-d-Galp-(1→x)]n-d-Glcp type. Carbohydrate Research 2014b, 400, 54-58. van Leeuwen, S. S.; Kuipers, B. J. H.; Dijkhuizen, L.; Kamerling, J. P., Comparative structural characterization of 7 commercial galacto-oligosaccharide (GOS) products. Carbohydrate Research 2016, 425, 48-58. Varki, A.; Cummings, R. D.; Aebi, M.; Packer, N. H.; Seeberger, P. H.; Esko, J. D.; Stanley, P.; Hart, G.; Darvill, A.; Kinoshita, T., Symbol nomenclature for graphical representations of glycans. Glycobiology 2015, 25, 1323-1324. Vera, C.; Córdova, A.; Aburto, C.; Guerrero, C.; Suárez, S.; Illanes, A., Synthesis and purification of galacto-oligosaccharides: state of the art. World Journal of Microbiology and Biotechnology 2016, 32, 197. Wang, Y.; Han, W.; Song, L.; Zhao, X., Compositional analysis and structural characterization of raffinose family oligosaccharides from Eupatorium. Journal of Food Composition and Analysis 2019, 84, 103298. Weichert, S.; Koromyslova, A.; Singh, B. K.; Hansman, S.; Jennewein, S.; Schroten, H.; Hansman, G. S., Structural Basis for Norovirus Inhibition by Human Milk Oligosaccharides. Journal of Virology 2016, 90, 4843-4848. Westphal, Y.; Schols, H. A.; Voragen, A. G. J.; Gruppen, H., Introducing porous graphitized carbon liquid chromatography with evaporative light scattering and mass spectrometry detection into cell wall oligosaccharide analysis. Journal of Chromatography A 2010, 1217, 689-695. White, D. R.; Hudson, P.; Adamson, J. T., Dextrin characterization by high-performance anion-exchange chromatography–pulsed amperometric detection and size-exclusion chromatography–multi-angle light scattering–refractive index detection. Journal of Chromatography A 2003, 997, 79-85. Wise, A.; Robertson, B.; Choudhury, B.; Rautava, S.; Isolauri, E.; Salminen, S.; Bode, L., Infants Are Exposed to Human Milk Oligosaccharides Already in utero. Front Pediatr 2018, 6, 270. Wu, S.; Grimm, R.; German, J. B.; Lebrilla, C. B., Annotation and Structural Analysis of Sialylated Human Milk Oligosaccharides. Journal of Proteome Research 2011, 10, 856-868. Wu, S.; Tao, N.; German, J. B.; Grimm, R.; Lebrilla, C. B., Development of an Annotated Library of Neutral Human Milk Oligosaccharides. Journal of Proteome Research 2010, 9, 4138-4151. Wu, Z.; Chen, L.; Wu, L.; Xue, X.; Zhao, J.; Li, Y.; Ye, Z.; Lin, G., Classification of Chinese Honeys According to Their Floral Origins Using Elemental and Stable Isotopic Compositions. Journal of Agricultural and Food Chemistry 2015, 63, 5388-5394. Xu, G.; Davis, J. C. C.; Goonatilleke, E.; Smilowitz, J. T.; German, J. B.; Lebrilla, C. B., Absolute Quantitation of Human Milk Oligosaccharides Reveals Phenotypic Variations during Lactation. The Journal of Nutrition 2017, 147, 117-124. Yan, J.; Ding, J.; Jin, G.; Duan, Z.; Yang, F.; Li, D.; Zhou, H.; Li, M.; Guo, Z.; Chai, W.; Liang, X., Profiling of Human Milk Oligosaccharides for Lewis Epitopes and Secretor Status by Electrostatic Repulsion Hydrophilic Interaction Chromatography Coupled with Negative-Ion Electrospray Tandem Mass Spectrometry. Analytical Chemistry 2019, 91, 8199-8206. Yang, Y.-H.; Lee, K.; Jang, K.-S.; Kim, Y.-G.; Park, S.-H.; Lee, C.-S.; Kim, B.-G., Low mass cutoff evasion with qz value optimization in ion trap. Analytical Biochemistry 2009, 387, 133-135. Yin, H.; Bultema, J. B.; Dijkhuizen, L.; van Leeuwen, S. S., Reaction kinetics and galactooligosaccharide product profiles of the β-galactosidases from Bacillus circulans, Kluyveromyces lactis and Aspergillus oryzae. Food Chemistry 2017, 225, 230-238. Yu, Z.-T.; Chen, C.; Newburg, D. S., Utilization of major fucosylated and sialylated human milk oligosaccharides by isolated human gut microbes. Glycobiology 2013, 23, 1281-1292. Zhang, J.; Song, G.; Mei, Y.; Li, R.; Zhang, H.; Liu, Y., Present status on removal of raff inose family oligosaccharides–a Review. Czech Journal of Food Sciences 2019, 37, 141-154. Zhang, R.; Zhao, Y.; Sun, Y.; Lu, X.; Yang, X., Isolation, Characterization, and Hepatoprotective Effects of the Raffinose Family Oligosaccharides from Rehmannia glutinosa Libosch. Journal of Agricultural and Food Chemistry 2013, 61, 7786-7793. Zhou, S.; Huang, Y.; Dong, X.; Peng, W.; Veillon, L.; Kitagawa, D. A. S.; Aquino, A. J. A.; Mechref, Y., Isomeric Separation of Permethylated Glycans by Porous Graphitic Carbon (PGC)-LC-MS/MS at High Temperatures. Analytical Chemistry 2017, 89, 6590-6597. Zhuang, D.; Qin, J.; Wang, H.-y.; Zhang, Y.; Liu, C.-y.; Ding, Q.-q.; Lv, G.-p., Oligosaccharide-based quality evaluation of Atractylodis rhizome and a strategy for simplifying its quality control. BMC Chemistry 2019, 13, 92. | - |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/92077 | - |
dc.description.abstract | 非消化性寡醣是低分子量的膳食纖維,其益生質效能取決於結構組成,然而非消化性寡醣的結構複雜性造成分析上的困難,本研究設計了多元的分析策略藉此克服此挑戰,以多孔性石墨化碳液相層析有效地分離了多數量的異構物,以還原法區別還原性與非還原性結構,並以四級桿串聯軌道阱式質譜儀的工作模式有效地鑑別高聚合度結構,已成功開發了非消化性寡醣的高通量分析平台,平台可鑑別38種異麥芽寡醣雙醣至六醣、29種清酒中雙醣至八醣、24種β-葡萄糖苷酶轉醣產物、84種半乳寡醣雙醣至六醣、47種蜂蜜中的二三醣、30種母乳寡醣三醣至九醣,相較過去研究能鑑別更多的寡醣結構。分析平台可應用於建立食品中的寡醣指標性成分,多元樣品分析結果顯示,異麥芽寡醣原料具有5組主要的雙醣至四醣成分群,以1→4與1→6鍵結構成,佔總寡醣量的55%以上; 清酒中的主要寡醣包含isomaltose、isomaltotriose、panose、63 glucosyl panose、isomaltotetraose、66 glucosyl maltohexaose,於相同製造商不同等級清酒中含量差異大; 半乳寡醣原料具有15組主要的雙醣至五醣成分群,大多為多元鍵結型態的還原性直線型結構,佔總寡醣量65%以上,相同製造商不同生產批次的原料發現了寡醣含量變異的情形; 洋槐、荔枝、龍眼、麥盧卡蜂蜜中寡醣譜型差異大,其差異來自於turanose、maltulose、isomaltose、maltose、nigerose、erlose。分析平台也可應用於探討寡醣的酵素合成特異性,β-葡萄糖苷酶Td2F2以cellobiose為受質之轉醣反應分析結果顯示,產物sophorose、laminaribiose、gentiobiose、α,β-trehalose先後抵達最大產率,且非還原端帶有β-1→6鍵結的結構不易被水解,造成產物累積。本研究所開發的PGC-LC-Orbitrap-MS/MS分析平台未來更可應用於探討寡醣結構與益生質效能的關聯性。 | zh_TW |
dc.description.abstract | Non-digestible oligosaccharides (NDO) are low-molecular-weight dietary fibers. The prebiotic efficacy is dependent on their chemical profile. However, profiling analysis of non-digestible oligosaccharides is still challenging due to structural complexity. We adopted multiple analytical strategies to overcome this challenge. Porous graphitic carbon liquid chromatography (PGC-LC) effectively separates numerous isomers. The reduction method differentiates non-reducing structures from reducing structures. The working mode of Q-Orbitrap facilitates the structural characterization of structures with a high degree of polymerization (DP). The high-throughput analytical platform for NDO was developed. The platform can characterize 38 isomalto-oligosaccharides (IMO) with DP2-DP6, 29 oligosaccharides in Sake with DP2-DP8, 24 transglycosylation products of β-glucosidase, 84 galacto‐oligosaccharides (GOS) with DP2-DP6, 47 oligosaccharides in honey with DP2-DP3, and 30 human milk oligosaccharides (HMO) with DP3-DP9. The platform is able to characterize more structures than previous studies. The platform can be applied to investigate marker oligosaccharides in foods. In IMO materials, we investigated five major DP2-DP4 with 1→4 and 1→6 linkages accounting for over 55% of total oligosaccharides content. In Sake samples, the main oligosaccharides were isomaltose and isomaltotriose, panose, 63 glucosyl panose, isomaltotetraose, and 66 glucosyl maltohexaose. Oligosaccharide profiles varied in Sake samples of different grades from the same manufacturer. In GOS materials, we investigated fifteen major group components up to DP5, accounting for at least 65% of total oligosaccharides content: most were linear structures with versatile linkages. Substantial variations in components occurred in GOS materials from different batches. In Acacia, Lychee, Longan, and Manuka honey samples, oligosaccharide profiles significantly varied. The difference contributed from turanose, maltulose, isomaltose, maltose, nigerose, and erlose. The platform can be also applied to study enzyme specificity of oligosaccharides synthesis. We studied the transglycosylation reaction using cellobiose catalyzed by β-glucosidase Td2F2. As a result, sophorose, laminaribiose, gentiobiose, and α,β-trehalose achieved maximum yield time successively. The structures with β-1→6 linkages at the non-reducing end were not easily hydrolyzed, leading to the accumulation of products. The developed platform can be further applied to investigate the structural and prebiotic-efficacy relationships in the future. | en |
dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2024-03-04T16:24:37Z No. of bitstreams: 0 | en |
dc.description.provenance | Made available in DSpace on 2024-03-04T16:24:37Z (GMT). No. of bitstreams: 0 | en |
dc.description.tableofcontents | 論文口試委員審定書 i
謝辭 ii 中文摘要 iv 英文摘要 v 圖目次 xv 表目次 xvii 附圖目次 xviii 附表目次 xx 壹、 前言 1 貳、 文獻回顧 2 1. 非消化性寡醣 2 1.1. 來源與結構多樣性 2 1.1.1. 異麥芽寡醣 2 1.1.2. 半乳寡醣 4 1.1.3. 果寡醣 4 1.1.4. 棉子糖半乳糖苷系列寡醣 5 1.1.5. 母乳寡醣 5 1.2. 結構與益生質效能之關聯 6 1.2.1. 醣組成 6 1.2.2. 鍵結型態 7 1.2.3. 聚合度 7 2. 高效能液相層析串聯質譜法於寡醣分析 8 2.1. 寡醣之衍生與結構修飾 9 2.1.1. 原態 9 2.1.2. 硼氫化鈉還原 9 2.1.3. 還原胺化 9 2.1.4. 全甲基化 10 2.1.5. 專一性酵素水解 10 2.2. 高效能液相層析於寡醣之分離 10 2.2.1. 陰離子交換層析 10 2.2.2. 親水性作用層析 11 2.2.3. 多孔性石墨化碳液相層析 11 2.3. 串聯質譜法於寡醣結構解析 12 2.3.1. 斷裂碎片命名法 12 2.3.2. 離子阱質譜儀 (Ion trap) 13 2.3.3. 四級桿串聯軌道阱式質譜儀 (Q-Orbitrap) 13 3. 非消化性寡醣分析之挑戰 15 3.1. 異麥芽寡醣 15 3.2. 半乳寡醣 15 3.3. 果寡醣 16 3.4. 棉子糖半乳糖苷系列寡醣 17 3.5. 母乳寡醣 17 參、 研究目的與架構 19 肆、 材料與方法 23 1. 實驗材料 23 1.1. 異麥芽寡醣原料 23 1.2. 日本清酒 23 1.3. 半乳寡醣原料 23 1.4. 蜂蜜 23 1.5. 母乳 24 2. 實驗藥品與溶劑 24 3. 寡醣標準品 25 4. 儀器設備 25 4.1. 高效能液相層析串聯質譜系統 25 4.2. 高效能陰離子交換層析-脈衝安培流檢測系統 26 5. β-葡萄糖苷酶Td2F2轉醣反應 26 5.1. 酵素表現 27 5.2. 酵素萃取與純化 27 5.3. 以pNP-β-D-Glc與葡萄糖為原料進行轉醣反應 28 5.4. 以cellobiose為原料進行轉醣反應 28 6. 以石墨化碳液相層析串聯軌道阱質譜法分析非消化性寡醣之結構組成 29 6.1. 樣品前處理 29 6.1.1. 異麥芽寡醣原料、半乳寡醣原料、蜂蜜 29 6.1.1.1. 原態樣品 (native) 29 6.1.1.2. Oligo-α-1,6-Glucosidase酵素水解異麥芽寡醣樣品 29 6.1.1.3. 還原後的半乳寡醣樣品 (reduced) 29 6.1.2. 清酒 30 6.1.3. 母乳 30 6.1.4. β-D-葡萄糖苷酶Td2F2轉醣反應產物 30 6.2. 檢量線製備 31 6.2.1. 用於定量異麥芽寡醣原料、清酒、半乳寡醣原料、蜂蜜、β-D-葡萄糖苷酶轉醣產物中寡醣 31 6.2.2. 用於定量嬰幼兒配方中半乳寡醣 32 6.2.3. 用於定量母乳中寡醣 32 6.3. 儀器分析 33 6.3.1. 異麥芽寡醣原料、清酒、半乳寡醣原料、蜂蜜中寡醣、β-葡萄糖苷酶轉醣產物 33 6.3.2. 母乳中寡醣 37 6.4. 寡醣波峰之標示規則 40 6.5. 寡醣結構與斷裂碎片離子之表示 40 6.6. 定量計算 40 6.6.1. 定量離子之選擇 40 6.6.2. 異麥芽寡醣、清酒、半乳寡醣、蜂蜜中寡醣、β-D-葡萄糖苷酶轉醣物 41 6.6.3. 母乳中寡醣 41 6.6.4. 重疊波峰之計算 42 6.7. 分析方法確效 42 6.7.1. 蜂蜜、異麥芽寡醣、半乳寡醣原料中寡醣 42 6.7.1.1. 線性 42 6.7.1.2. 重複性 42 6.7.2. 清酒中的寡醣 42 6.7.2.1. 線性 42 6.7.2.2. 重複性 43 6.7.3. 嬰幼兒配方中的半乳寡醣 43 6.7.3.1. 線性 43 6.7.3.2. 重複性與基質效應 43 6.7.3.3. 定量極限 43 6.7.4. 母乳中的寡醣 43 6.7.4.1. 線性 43 6.7.4.2. 重複性 43 6.7.4.3. 準確度 44 7. 石墨化碳液相層析串聯軌道阱質譜法的開發與條件優化 44 7.1. 母乳去脂去蛋白溶劑比例的評估 44 7.2. 母乳寡醣分析之內標選擇 45 7.3. 寡醣還原反應時間的評估 45 7.4. Oligo-α-1,6-Glucosidase鍵結專一性評估 45 7.5. 液相層析條件之優化 46 7.5.1. 蜂蜜中的寡醣 46 7.5.2. 半乳寡醣 46 7.5.3. 母乳寡醣 46 7.6. 質譜加成離子的選擇 46 7.6.1. 六碳醣寡醣 46 7.6.1. 母乳寡醣 46 7.7. 質譜電噴灑游離條件之優化 47 7.7.1. 六碳醣寡醣 47 7.7.2. 母乳寡醣 47 7.8. 質譜撞擊能量之優化 47 7.9. 已知結構寡醣標準品之分子斷裂模式分析 47 8. 以陰離子交換層析-脈衝安培流檢測法分析樣品中單醣含量 48 8.1. 樣品分析前處理 48 8.2. 檢量線製備 48 8.3. 儀器分析 48 8.4. 定量計算 49 9. 統計試驗 49 9.1. 比較平均數法 49 9.2. Partial least squares discriminate analysis 49 伍、 結果與討論 51 1. 寡醣鍵結判定規則之建立: 標準品斷裂模式與滯留時間之分析 51 1.1. 斷裂模式分析 51 1.1.1. 還原性直線型寡醣 (reducing linear oligosaccharides) 51 1.1.2. 還原性分支型寡醣 52 1.1.3. 非還原性直線型寡醣 53 1.2. 鍵結判定規則統整 53 1.3. 滯留時間於判別醣組成、鍵結型態與鍵結首旋之適用性 55 2. 寡醣之結構鑑別之展示 55 2.1. 基本假設 56 2.2. 鑑別波峰重疊之異構物 57 2.3. 異麥芽寡醣與半乳寡醣中較高聚合度的還原性直線型結構 57 2.4. 異麥芽寡醣與半乳寡醣中相同鍵結形式的異構物群 58 2.5. 異麥芽寡醣與半乳寡醣中的分支結構 (分支位於還原端) 60 2.6. 清酒中的分支結構 (分支不位於還原端) 63 2.7. 蜂蜜與半乳寡醣中的非還原性直線型結構 63 2.8. 母乳寡醣中相同醣組成相異鍵結形式的異構物群 65 3. 非消化性寡醣之結構組成分析 67 3.1. 異麥芽寡醣原料 67 3.1.1. 結構多樣性 67 3.1.2. 聚合度分布 68 3.1.3. 主要成分 68 3.1.4. 不同來源樣品之譜型差異 69 3.2. 日本清酒 70 3.2.1. 結構多樣性 70 3.2.2. 聚合度分布 71 3.2.3. 主要成分 72 3.2.4. 不同等級清酒之譜型差異 72 3.3. 半乳寡醣原料 73 3.3.1. 結構多樣性 73 3.3.2. 聚合度分布 74 3.3.3. 主要成分 75 3.3.4. 不同來源樣品之譜型差異 76 3.4. 蜂蜜中的寡醣 78 3.4.1. 結構多樣性 78 3.4.2. 聚合度分布 79 3.4.3. 不同蜜源樣品之寡醣分布差異性與多變量分析 80 3.5. 母乳中的母乳寡醣 82 3.5.1. 結構多樣性 82 3.5.2. 主要成分 83 3.5.3. 產後1-52周的濃度變化 83 4. β-葡萄糖苷酶Td2F2轉醣反應特異性 85 4.1. 轉醣產物結構多樣性 85 4.2. 以pNP-β-D-Glc與葡萄糖為原料之轉醣反應特異性 86 4.3. 以cellobiose為原料之轉醣反應特異性 86 5. 寡醣分析方法確效 88 5.1. 異麥芽寡醣原料的寡醣 88 5.1.1. 檢量線線性 88 5.1.2. 重複性 88 5.2. 清酒中的寡醣 89 5.2.1. 檢量線線性 89 5.2.2. 重複性 89 5.3. 半乳寡醣原料與嬰幼兒配方中的半乳寡醣 89 5.3.1. 檢量線線性 89 5.3.2. 重複性 90 5.3.3. 基質效應 90 5.3.4. 定量極限 91 5.4. 蜂蜜中的寡醣 91 5.4.1. 檢量線線性 91 5.4.2. 重複性 91 5.5. 母乳中的母乳寡醣 92 5.5.1. 檢量線線性 92 5.5.2. 重複性 92 5.5.3. 準確度 92 5.6. β-葡萄糖苷酶Td2F2轉醣產物 93 5.6.1. 檢量線線性 93 6. 寡醣分析方法開發與分析條件優化 94 6.1. 去脂肪去蛋白溶劑比例的優化 94 6.2. 內標的選擇 94 6.3. 還原反應時間之優化 95 6.4. 酵素鍵結專一性評估 95 6.5. 液相層析條件之優化 96 6.5.1. 蜂蜜中的寡醣 97 6.5.2. 半乳寡醣與異麥芽寡醣 98 6.5.3. 母乳寡醣 99 6.6. 質譜加成離子的選擇 101 6.7. 質譜電噴灑游離條件之優化 102 6.8. 質譜撞擊能量之優化 103 7. PGC-LC-Orbitrap-MS/MS寡醣分析方法之應用性 104 7.1. 建立食品寡醣指標性成分 104 7.2. 寡醣合成特異性研究 105 7.3. 未來應用潛力: 探討寡醣結構與益生質效能之關聯性 105 陸、 結論 107 柒、 參考文獻 109 捌、 縮寫 122 玖、 圖 123 壹拾、 表 163 壹拾壹、 附錄 184 1. 附圖 184 2. 附表 215 | - |
dc.language.iso | zh_TW | - |
dc.title | 以石墨化碳液相層析串聯軌道阱質譜法探討非消化性寡醣之多樣性結構組成 | zh_TW |
dc.title | Profile Diversity of Non-Digestible Oligosaccharides Revealed by Porous Graphitic Carbon Liquid Chromatography-Orbitrap Tandem Mass Spectrometry | en |
dc.type | Thesis | - |
dc.date.schoolyear | 112-1 | - |
dc.description.degree | 博士 | - |
dc.contributor.oralexamcommittee | 羅翊禎;陳宏彰;陳明煦;張永和;楊登傑;陳頌方 | zh_TW |
dc.contributor.oralexamcommittee | Yi-Chen Lo;Hong-Jhang Chen;Ming-Hsu Chen;Yung-Ho Chang;Deng-Jye Yang;Sung-Fang Chen | en |
dc.subject.keyword | 多孔性石墨化碳液相層析串聯軌道阱質譜法,非消化性寡醣,異麥芽寡醣,半乳寡醣,β-葡萄糖苷酶轉醣產物,母乳寡醣, | zh_TW |
dc.subject.keyword | porous graphitic carbon liquid chromatography-orbitrap tandem mass spectrometry,non-digestible oligosaccharides,isomalto-oligosaccharides,galacto-oligosaccharides,transglycosylation products of β-glucosidase,human milk oligosaccharides, | en |
dc.relation.page | 291 | - |
dc.identifier.doi | 10.6342/NTU202400581 | - |
dc.rights.note | 未授權 | - |
dc.date.accepted | 2024-02-14 | - |
dc.contributor.author-college | 生物資源暨農學院 | - |
dc.contributor.author-dept | 食品科技研究所 | - |
顯示於系所單位: | 食品科技研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-112-1.pdf 目前未授權公開取用 | 14.17 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。