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標題: | 嬰兒糞便的短鏈脂肪酸組成與異位性皮膚炎之相關性 The correlation between the fecal short-chain fatty acids composition and atopic dermatitis in infants |
作者: | 李佩儒 Pei-Ru Li |
指導教授: | 林璧鳳 Bi-Fong Lin |
關鍵字: | 異位性皮膚炎,短鏈脂肪酸,膳食纖維,糞便,飲食, atopic dermatitis,short-chain fatty acids,dietary fiber,feces,diet, |
出版年 : | 2024 |
學位: | 碩士 |
摘要: | 異位性皮膚炎 (atopic dermatitis, AD) 是一種常見的慢性發炎皮膚疾病,尤其在台灣AD的發病率持續上升,主要好發於嬰兒及幼童。AD所導致的皮膚乾癢、紅腫等症狀對嬰幼兒的健康產生嚴重影響。近年來,AD發病機制越來越關注到腸道微生物,特別是其代謝產物短鏈脂肪酸 (short-chain fatty acids, SCFAs)。已經有證據表明SCFAs在調節免疫反應和維護皮膚健康方面扮演關鍵角色。因此,本研究旨在探討嬰兒糞便中的短鏈脂肪酸組成,包括與異位性皮膚炎之間的相關性。本研究與台大小兒過敏門診合作,收集6個月齡的嬰兒糞便樣本,並分析AD組 (n=70) 和對照組 (Ctrl)(n=49) 嬰兒的糞便短鏈脂肪酸濃度。同時,也收集嬰兒近1個月的飲食分析表,計算其膳食纖維攝取量。首先以氣相層析-質譜 (gas chromatography-mass spectrometry, GC-MS) 建立糞便短鏈脂肪酸檢測方法,並進行確效測試。結果顯示,使用0.25% HCl ACN前處理的各個短鏈脂肪酸在檢量線濃度範圍內呈現良好的線性關係 (R2>0.99),糞便樣品無明顯的基質效應,且具有良好的精密度及準確度,符合化學檢驗法的確效規範。依據此方法測得嬰兒糞便乙酸 (C2) 濃度為17.3-192 μmol/g、丙酸 (C3) 濃度為0.21-31 μmol/g、丁酸 (C4) 濃度為0-9 μmol/g,而戊酸與己酸則未能在糞便中檢測。與Ctrl組相比,AD組嬰兒的糞便乙酸、C2+C3+C4的濃度顯著較低,丙酸、丁酸也有較低的趨勢。另外,丙酸濃度與AD的疾病嚴重程度呈負相關。嬰兒飲食調查的結果發現,中度AD組嬰兒的膳食纖維攝取量顯著低於控制組,且膳食纖維量與AD的疾病嚴重程度呈負相關;嬰兒飲食內的膳食纖維量與糞便內乙酸、丙酸、C2+C3+C4呈正相關。此外,也有發現嬰兒飲食補充益生菌可提高糞便內丙酸、丁酸的濃度。綜上所述,罹患AD的嬰兒具有較低的糞便短鏈脂肪酸,而飲食的膳食纖維與益生菌,可能有助於提高短鏈脂肪酸濃度。 Atopic dermatitis (AD) is a common chronic inflammatory skin disorder, and its incidence has been steadily increasing in Taiwan, particularly among infants and young children. The symptoms of AD, such as dry and inflamed skin, can severely impact health. In recent years, research on the pathogenesis of AD has increasingly focused on the gut microbiota, specifically its metabolic products known as short-chain fatty acids (SCFAs). Evidence suggests that SCFAs are crucial in regulating immune responses and maintaining skin health. Therefore, this study aims to investigate the correlation between the composition of fecal SCFAs and atopic dermatitis in infants. This study collaborated with the Division of Pediatric Allergy of NTU hospital, collecting fecal samples from 6-month-old infants and analyzing the concentrations of SCFAs in both AD group (n=70) and control group (Ctrl) (n=49) of infants. Dietary intake data for infants were also collected to analyze their dietary fiber intake. The fecal SCFAs detection method was established using gas chromatography-mass spectrometry (GC-MS), and a validation test was conducted. The results showed that SCFAs, pretreated with 0.25% HCl ACN, exhibited good linear relationships of the calibration curves (R2>0.999), with no significant matrix effects in fecal samples. The method also demonstrated good precision and accuracy, complying with the validation guidelines. Using this method, the concentration of acetic acid (C2) in infant feces was measured to be 17.3-192 μmol/g, propionic acid (C3) ranged from 0.21-31 μmol/g, and butyric acid (C4) ranged from 0-9 μmol/g. However, valeric acid and caproic acid were not detected in feces. When compared to the control group, infants in the AD group had significantly lower concentration of acetic acid, C2+C3+C4, and a trend of lower propionic acid and butyric acid in feces. Furthermore, there was a negative correlation between propionic acid and the severity of AD. Dietary analysis showed that those with moderate AD had significantly lower dietary fiber intake, and dietary fiber intake was negatively correlated with the severity of AD. The amount of dietary fiber was positively correlated with fecal acetic acid, propionic acid, and C2+C3+C4 concentration. Additionally, it was observed that supplementing infants' diets with probiotics increased fecal propionic acid and butyric acid concentration. In summary, infants with AD had lower fecal SCFAs levels. Dietary fiber and probiotics in their diets may help increase SCFAs concentration. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/91695 |
DOI: | 10.6342/NTU202400145 |
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顯示於系所單位: | 生化科技學系 |
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