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標題: | 秀麗隱桿線蟲伴侶辨識神經迴路和其分子機制研究 Circuit Mechanism of Behavioral States for Mate Recognition in C. elegans Males |
作者: | 游凱安 Kai-An You |
指導教授: | 陳俊豪 Chun-Hao Chen |
關鍵字: | 輻射狀神經,伴侶辨識,感覺誘發之行為改變,谷氨酸信號,持續性行為狀態, ray-neurons,mating recognition,sensory-evoked behavioral states,glutamate signal,persistent behavioral states, |
出版年 : | 2023 |
學位: | 碩士 |
摘要: | 動物會整合環境變化和生理需求並做出行為反應,從而提供靈活和持久的適應行為。 先前的研究表明,雄性秀麗隱桿線蟲在接觸合適的伴侶時表現出行動範圍的下降,而其神經機制仍須進一步研究。在這篇研究中表明,雄性線蟲在辨識到合適的伴侶,會誘發持久性的行動範圍下降,其藉由增加移動中的後退,來達到限制行動的範圍。先前已得知是藉由雄性尾巴的 B型輻射狀神經與伴侶的物理接觸,可以驅使雄性線蟲從大範圍的移動轉變為小範圍搜索,並且其行為改變無須環境中的費洛蒙;此外我們也發現,對於雄性尾巴的 B型輻射狀神經 (RnBs)利用光遺傳學的方式刺激神經活性,再現了反復後退和搜索範圍降低的持續性行為變化。我們發現谷氨酸能LUA中間神經元對於維持刺激RnBs所誘導的降低搜索範圍的行動是必要的。經由分析神經連結表明,LUA 可能通過多個谷氨酸受體在神經元之間傳遞谷氨酸信號,進而引發反覆後退、降低搜索範圍的行為狀態。我們發現CRF-like GPCR 受體中的SEB-3蛋白也參與行為狀態的改變。我們的研究揭示了雄性在接觸合適的伴侶後誘發的局部搜索行為是藉由谷氨酸神經迴路來傳遞,並提供了感覺誘發的行為狀態改變下的神經分子機制。 Animals integrate internal needs and environmental cues to display behavioral states that offer flexible, scalable, and persistent actions for adaptations. Previous studies indicate that C. elegans males show a long-term reduction of locomotion coverage upon contacting suitable mates, while the circuit mechanism remains unknown. Here, we find that brief contact with suitable mates is able to evoke a persistent behavioral state that abruptly restricts locomotion coverage by increasing reversals for minutes. The local search state requires physical contact by male tails but not pheromones, suggesting that tail sensilla are needed. Indeed, transient optogenetic activation of ray-type B neurons (RnBs), a group of sensory neurons for contact response in males, recapitulates persistent behavioral changes associated with the recurrent activity. In addition, we show that glutamatergic LUA interneurons are necessary to sustain the local search state evoked by RnBs activation. Genetic analyses indicate that LUAs likely distribute glutamate signals through multiple glutamatergic receptors for repeated reversals. Moreover, we found CRF-like GPCR receptor SEB-3 is involved in the altered behavioral state. Furthermore, we found that glutamate mutants are in the persistence of restrictive behavior. Our study thus uncovers a glutamatergic circuit underlying the local search state of males upon contacting suitable mates and provides molecular insights into neural mechanisms of sensory-evoked behavioral states. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/91476 |
DOI: | 10.6342/NTU202302062 |
全文授權: | 同意授權(全球公開) |
顯示於系所單位: | 分子與細胞生物學研究所 |
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