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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鄭光成 | zh_TW |
| dc.contributor.advisor | Kuan-Chen Cheng | en |
| dc.contributor.author | 洪翎 | zh_TW |
| dc.contributor.author | Ling Hong | en |
| dc.date.accessioned | 2023-10-03T16:32:52Z | - |
| dc.date.available | 2023-11-09 | - |
| dc.date.copyright | 2023-10-03 | - |
| dc.date.issued | 2023 | - |
| dc.date.submitted | 2023-08-08 | - |
| dc.identifier.citation | 黃亦尊 (2015) 開發普魯藍抗菌活性包材用於延長冷凍鯛魚片保存期限之研究。國立臺灣大學食品科技研究所學位論文。台北。台灣
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/90540 | - |
| dc.description.abstract | 活性包裝材料係指透過與食品的相互作用來延長食品的儲藏期限，以減少、延緩甚至達到抑制腐敗及病原微生物的生長。本研究旨在開發具多孔狀細菌性纖維 (bacterial cellulose, BC) 與幾丁聚醣 (chitosan) 製成之活性包材，除了評估其材料特性外，亦評估其作為活性包材用於金目鱸魚片保存之應用。結果顯示以原位修飾法透過1%的乳化劑、0.2%的三仙膠、chitosan 與培養基形成泡沫狀培養基，後加入 Komagataeibacter xylinus 以生產具有微孔結構 BC，於材料特性分析上，利用掃描電子顯微鏡和傅立葉轉換紅外光譜確認了 BC 之奈米纖維與 chitosan 之間的鍵結形成，而 X 光繞射分析顯示 chitosan 的添加影響了其結晶度，從73%下降至69%，於溶脹試驗中可發現多孔狀 BC 組別具有高達2,300%之溶脹度，表示其具有高吸收液體之能力。抗菌性實驗結果顯示添加 chitosan 之 BC 組別可顯著降低 Staphylococcus aureus 和 Escherichia coli 約2個對數之菌落形成單位，綜合以上結果可知多孔狀 BC/ chitosan 確實具備高吸收性與抗菌性，有助於發展成為抗菌性活性包材，目前仍持續進行金目鱸魚片保存試驗，以評估其實際應用性。 | zh_TW |
| dc.description.abstract | Active packaging refers to materials that interact with food to extend the shelf life to reduce, delay, or inhibit the growth of spoilage and pathogenic microorganisms. This study aims to develop active packaging materials using porous bacterial cellulose (BC) and chitosan. The material properties and their application as active packaging for preserving Lates calcarifer fillets were evaluated. The results showed that a foaming medium was formed by in-situ modification using 1% cremodan, 0.2% xanthan, chitosan, and the culture medium, followed by the addition of Komagataeibacter xylinus to produce BC with a porous structure. Scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR) confirmed the formation of bonds between the nanofibers of BC and chitosan, and X-ray diffraction analysis showed that the addition of chitosan affected its crystallinity, reducing it from 73% to 69%. In the swelling ratio test, the porous BC exhibited a high swelling capacity up to 2,300%, indicating its high liquid absorption ability. The antibacterial experiment showed that the foaming BC (FBC) with chitosan added significantly reduced Staphylococcus aureus and Escherichia coli by approximately 2 logCFU/mL. Based on the above results, it can be concluded that FBC/chitosan possesses high absorbency and antibacterial properties, making it suitable for the development of antibacterial active packaging. The preservation tests on fish fillets are still ongoing to evaluate its applicability. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-10-03T16:32:52Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2023-10-03T16:32:52Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 口試委員會審定書 I
謝誌 II 摘要 III Abstract IV 目錄 V 圖目錄 VIII 表目錄 IX List of Figures X List of Tables XI 壹、 前言 1 貳、 文獻回顧 2 2.1 食品安全衛生安全 2 2.1.1 腸炎弧菌 4 2.1.2 金黃色葡萄球菌 4 2.1.3 大腸桿菌 5 2.1.4 腐敗希瓦氏菌 5 2.2 食品包裝材料 6 2.2.1 智能包裝材料 7 2.2.2 活性包裝材料 8 2.2.3 食品包材之應用 10 2.2.3.1 延長水果和蔬菜的保質期 10 2.2.3.2 延長魚類和肉類的保質期 10 2.3 細菌性纖維素 11 2.3.1 細菌性纖維素生產機制 13 2.3.2 細菌纖維素修飾方法 14 2.3.3 木質醋酸菌 18 2.3.4 細菌纖維素應用 19 2.4 幾丁聚醣 21 2.4.1 幾丁聚醣特性 21 2.4.2 幾丁聚醣應用 23 2.4.3 幾丁聚醣抗菌性 25 2.5 相關法規 26 參、 研究目的與架構 29 3.1 研究目的 29 3.2 研究架構 29 肆、 材料與方法 31 4.1 實驗材料 31 4.1.1 實驗菌株及細胞 31 4.1.2 實驗原料 31 4.1.3 實驗藥品 31 4.2 儀器設備 33 4.3 實驗方法 34 4.3.1 菌種保存 34 4.3.2 一般細菌纖維素製作 34 4.3.3 泡沫細菌纖維素 (Foaming bacterial cellulose, FBC) 製作 34 4.4 最適化生產幾丁聚醣-泡沫細菌纖維素 36 4.4.1 不同氮源濃度條件下對泡沫細菌纖維素產量的影響 36 4.4.2 不同碳源濃度條件下對泡沫細菌纖維素產量的影響 36 4.4.3 不同幾丁聚醣濃度條件下對泡沫細菌纖維素產量的影響 36 4.5 掃描式電子顯微鏡 (scanning electron microscope, SEM) 36 4.6 傅立葉轉換紅外線光譜 (fourier transform infrared spectroscopy, FTIR) 37 4.7 X射線繞射分析儀 (X-ray Diffraction, XRD) 37 4.8 熱重分析儀測定 (Thermogravimetric Analyzer, TGA) 37 4.9 溶脹能力測試 (Swelling ratio) 38 4.10 機械性質測定 (Mechanical properties) 38 4.11 抗菌試驗 39 4.12 細胞毒性分析 39 4.13 金目鱸魚儲藏性實驗 39 4.14 統計分析 42 伍、 結果與討論 43 5.1 最適化生產幾丁聚醣-泡沫細菌纖維素 43 5.1.1 不同氮源濃度條件下對泡沫細菌纖維素產量的影響 43 5.1.2 不同碳源濃度條件下對泡沫細菌纖維素產量的影響 44 5.1.3 不同幾丁聚醣濃度條件下對泡沫細菌纖維素產量的影響 46 5.2 掃描式電子顯微鏡分析 47 5.3 傅立葉轉換紅外線光譜分析 49 5.4 X 射線繞射分析 51 5.5 熱重分析 53 5.6 機械性質分析 55 5.7 吸水率與溶脹能力測試 57 5.8 細胞毒性分析 60 5.9 抑菌效果分析 62 5.10 金目鱸魚儲藏性實驗 63 5.10.1 包裝金目鱸魚片微生物分析 63 5.10.2 總揮發性鹽基態氮(TVBN) 64 5.10.3 硫代巴比妥酸反應物質(TBARS) 65 陸、 結論與未來展望 67 柒、 參考文獻 68 捌、附錄 85 | - |
| dc.language.iso | zh_TW | - |
| dc.title | 以幾丁聚醣修飾之泡沫培養基生產多孔性細菌纖維素作為活性包材之應用 | zh_TW |
| dc.title | In situ modification of bacterial cellulose utilizing foaming medium with chitosan and its application in food packaging | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 111-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.coadvisor | 林欣平 | zh_TW |
| dc.contributor.coadvisor | Shin-Ping Lin | en |
| dc.contributor.oralexamcommittee | 謝寶全;陳錦樹;陳俊太 | zh_TW |
| dc.contributor.oralexamcommittee | Pao-chuan Hsieh;Chin-Shun Chen;Jiun-Tai Chen | en |
| dc.subject.keyword | 泡沫細菌性纖維素,幾丁聚醣,活性包裝,抗菌性,吸水墊料, | zh_TW |
| dc.subject.keyword | foaming bacterial cellulose,chitosan,absorbent pads,antimicrobial,active food packaging, | en |
| dc.relation.page | 115 | - |
| dc.identifier.doi | 10.6342/NTU202303053 | - |
| dc.rights.note | 未授權 | - |
| dc.date.accepted | 2023-08-09 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 食品科技研究所 | - |
| 顯示於系所單位： | 食品科技研究所 | |
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