丁酸對血管內皮細胞發炎介質、細胞自噬、凝血與鈣化相關基因與蛋白表現之影響

Abstract

丁酸 (Butyric acid)屬於短鏈脂肪酸(Short Chain Fatty Acids)的一種，是牙髓以及牙周致病菌代謝纖維食物後之產物，且已經被文獻提出會在受感染的牙髓及根管系統及牙齦溝液中被測得相當之濃度，且證實與牙髓炎／牙根尖炎及牙周炎的發生與進行有相當之關聯；人類血管內皮細胞(human vascular endothelial cell)被視為與血管新生、牙髓及牙周組織的發炎和癒合扮演重要角色；然而丁酸是否會誘發血管內皮細胞的發炎反應、細胞自噬、凝血反應以及血管鈣化，進而在牙髓炎與牙周炎致病機轉之角色，到目前為止鮮少有研究與討論，也是本研究之重要目的。

Butyric acid, as one kind of short chain fatty acids (SCFAs) and is mainly secreted as a metabolic product of fibrous food by the bacteria in the periodontal pocket/pulpal-root canal. It has been reported to be elevated to significant concentrations in the contaminated root canal systems, as well as in the gingival crevicular fluid. Higher SCFAs are confirmed to be significantly associated with the occurrence and progression of pulpitis/apical periodontitis and periodontitis. Human vascular endothelial cells are considered to play an important role in angiogenesis, inflammation, and healing of dental pulp and periodontal tissue. However, whether butyrate induces inflammatory responses, autophagy, coagulation, and vascular calcification in endothelial cells and its role in the pathogenesis of pulpitis and periodontitis has been rarely studied and discussed, and is also an important objective of this study.