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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃念祖 | zh_TW |
| dc.contributor.advisor | Nien-Tsu Huang | en |
| dc.contributor.author | 辛旻諺 | zh_TW |
| dc.contributor.author | Min-Yen Hsin | en |
| dc.date.accessioned | 2023-09-22T17:02:39Z | - |
| dc.date.available | 2023-11-09 | - |
| dc.date.copyright | 2023-09-22 | - |
| dc.date.issued | 2023 | - |
| dc.date.submitted | 2023-08-10 | - |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/90010 | - |
| dc.description.abstract | 本論文介紹了一種微流體裝置，用於研究缺氧和剪應力對內皮細胞行為的影響。內皮細胞在炎症、心血管疾病和癌症生長中發揮著關鍵作用。該微流體裝置能夠精確控制細胞培養室內的氧氣濃度分佈和剪應力大小，實現多重測試。通過將內皮細胞暴露在不同的缺氧和剪應力環境下，通過測量反應性氧化物種（ROS）以評估細胞內的氧化壓力，並進行細胞形態分析。研究結果表明，缺氧和剪應力可以調節ROS的產生，為心血管疾病和運動相關研究提供了重要見解。該裝置提供了比傳統方法更貼近生理的細胞培養環境，並可應用於抗癌藥物測試、腫瘤學研究和心血管疾病調查等領域。總體而言，該微流體裝置可作為瞭解內皮細胞對缺氧和剪應力反應機制的研究工具，其對氧氣濃度分佈和剪應力條件的精確控制有助於全面研究這些因素的影響，並推動血管生物學和相關學科的發展。 | zh_TW |
| dc.description.abstract | This thesis introduces a microfluidic device developed to investigate the influence of hypoxia and shear stress on endothelial cell behavior. Endothelial cells are critical in inflammation, cardiovascular diseases, and cancer growth. The microfluidic device enables precise control of oxygen distribution and shear stress conditions within a cell culturing chamber, facilitating multiplex testing. By subjecting endothelial cells to varying hypoxia and shear stress levels, intracellular oxidative stress was evaluated through reactive oxygen species (ROS) measurements and cell morphology analysis. The findings indicate that hypoxia and shear stress can modulate ROS expression, offering valuable insights into vascular diseases and exercise-related research. The device provides a more physiologically relevant cell culturing environment than conventional methods, and its potential applications extend to anti-cancer medication testing, oncological studies, and cardiovascular disease investigations. In summary, the developed microfluidic device is a valuable tool for understanding the underlying mechanisms governing endothelial cell behavior in response to hypoxia and shear stress. The precise control over oxygen distribution and shear stress conditions facilitates comprehensive investigations into the effects of these factors, thereby contributing to advancements in the field of vascular biology and related disciplines. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-09-22T17:02:39Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2023-09-22T17:02:39Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | CONTENTS
口試委員會審定書 # 誌謝 i 中文摘要 ii ABSTRACT iii CONTENTS iv LIST OF FIGURES vii LIST OF TABLES xii Chapter 1 Introduction 1 1.1 Background 1 1.2 Literature review 3 1.2.1 Shear stress stimulation for cells response studies 3 1.2.2 Hypoxia condition generation for cell incubation 7 1.2.3 Oxygen level measurement in microfluidic devices 11 1.2.4 Reactive oxygen species (ROS) 13 1.2.5 Summary 16 1.3 Research motivation 20 1.4 Thesis structure 21 Chapter 2 Experimental Design 22 2.1 The microfluidic channel 22 2.1.1 Navier-Stokes equation 22 2.1.2 Shear stress 23 2.1.3 Oxygen distribution 24 2.2 Endothelial cells 25 2.3 Widefield frequency domain fluorescence lifetime imaging microscopy (FD-FLIM) 26 2.3.1 Lifetime of fluorescence 26 2.3.2 System setup 26 Chapter 3 Materials and Methods 28 3.1 The microfluidic channel designs 28 3.2 Mold and device fabrication 29 3.2.1 PDMS soft lithography 29 3.3 Pump setup design 32 3.4 Flow control and hypoxia condition generation 33 3.5 Cell line and culturing process 35 3.6 Fluorescent staining and microscopy process 36 3.7 Reactive oxygen species (ROS) measurement 37 3.8 Image acquisition 37 Chapter 4 Results and Discussion 39 4.1 Simulation results of the oxygen distribution profile and shear stress 39 4.2 FLIM measurement results 42 4.3 Cell image 43 4.3.1 Cell image processing procedure 43 4.3.2 Viability 44 4.3.3 Confluency 45 4.3.4 Orientation and morphology analysis 46 4.3.5 ROS generation 49 Chapter 5 Conclusion 52 Chapter 6 Future Work 54 Reference 56 Abbreviation 60 | - |
| dc.language.iso | en | - |
| dc.title | 可提供缺氧環境及剪應力刺激之微流道裝置對於血管內皮細胞型態及活性氧化物表現之研究 | zh_TW |
| dc.title | A microfluidic device to study the coupling effect of hypoxia and shear stress on morphology and reactive oxygen species secretion of endothelial cells | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 111-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 董奕鍾;楊東霖;郭柏齡 | zh_TW |
| dc.contributor.oralexamcommittee | Yi-Chung Tung;T-Tony Yang;Po-Ling Kuo | en |
| dc.subject.keyword | 缺氧,剪應力,微流道,內皮細胞, | zh_TW |
| dc.subject.keyword | Hypoxia,Shear stress,Microfluidic,Endothelial, | en |
| dc.relation.page | 60 | - |
| dc.identifier.doi | 10.6342/NTU202303662 | - |
| dc.rights.note | 未授權 | - |
| dc.date.accepted | 2023-08-11 | - |
| dc.contributor.author-college | 電機資訊學院 | - |
| dc.contributor.author-dept | 生醫電子與資訊學研究所 | - |
| 顯示於系所單位： | 生醫電子與資訊學研究所 | |
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