請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/87569完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 潘敏雄 | zh_TW |
| dc.contributor.advisor | Min-Hsiung Pan | en |
| dc.contributor.author | 鍾尚哲 | zh_TW |
| dc.contributor.author | Shang-Che Chung | en |
| dc.date.accessioned | 2023-06-20T16:07:01Z | - |
| dc.date.available | 2023-11-09 | - |
| dc.date.copyright | 2023-06-20 | - |
| dc.date.issued | 2023 | - |
| dc.date.submitted | 2023-02-15 | - |
| dc.identifier.citation | Abebe Alamineh, E. (2018). Extraction of pectin from orange peels and characterizing its physical and chemical properties. American Journal of Applied Chemistry, 6(2). doi:10.11648/j.ajac.20180602.13
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Apoptosis and apoptotic body: disease message and therapeutic target potentials. Biosci Rep, 39(1). doi:10.1042/BSR20180992 Yang, M., Liu, X., Luo, Q., Xu, L., & Chen, F. (2020). An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy. J. Nanobiotechnology, 18(1), 100. doi:10.1186/s12951-020-00656-9 Zhang, M., Viennois, E., Prasad, M., Zhang, Y., Wang, L., Zhang, Z., Han, M. K., Xiao, B., Xu, C., Srinivasan, S., & Merlin, D. (2016). Edible ginger-derived nanoparticles: A novel therapeutic approach for the prevention and treatment of inflammatory bowel disease and colitis-associated cancer. Biomaterials, 101, 321-340. doi:10.1016/j.biomaterials.2016.06.018 Zhang, M., Xiao, B., Wang, H., Han, M. K., Zhang, Z., Viennois, E., Xu, C., & Merlin, D. (2016). Edible ginger-derived nano-lipids loaded with doxorubicin as a novel drug-delivery approach for colon cancer therapy. Mol. 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/87569 | - |
| dc.description.abstract | 植物來源性胞外囊泡 (Plant-derived extracellular vesicles，簡稱PDEVs) 如其名為從植物分離出來的胞外囊泡，雖然PDEVs相關的研究剛起步沒有多少年，但已有研究指出其具有多種生物活性。近年來，從食用蔬果到植物根莖皆有發表成功分離出胞外囊泡的成果。薑黃是物種Curcuma longa的根莖，常用於烹飪和草藥中，為咖哩橘黃色的色素來源之一，薑黃的研究在學界已持續多年，已知這類橘黃色植物根莖具有多種生物活性，包括抗發炎、抗癌等，然而關於薑黃來源性胞外囊泡 (TEVs) 的研究相當少。此外，柑橘類水果常見於日常生活飲食中，以富含維生素 C 並具抗氧化及抗發炎功效聞名，已有研究成功從中分離出柑橘類胞外囊泡 (CEVs)，但從不同品種柑橘類分離出的胞外囊泡之間是否有差異則不明確。因此在本次研究中，我們目標從薑黃與不同品種柳橙中分離出胞外囊泡，並分析其性質和生物活性。藉由切向過濾法成功分離出 TEVs，CEVs 則是利用超高速離心與蔗糖梯度離心法純化得到，後續我們測定粒徑分布與界達電位來了解不同胞外囊泡間的奈米性質差異。分析不同成熟度來源的 TEVs 中發現了小片段RNA，且未成熟 TEVs 薑黃素(Curcumin, CU)含量較高。在細胞實驗中，TEVs 可以抑制大腸癌細胞株的增殖，然而CEVs 則否，同時從成熟薑黃中分離出的 TEVs 在抑制癌細胞生長的效果比未成熟薑黃的效果好。除此之外，成熟來源的 TEVs 在不殺死巨噬細胞的情況下，能夠抑制經 LPS 誘導後的 NO 產生量，但再製成空載體後這些現象就消失。這些結果得出 TEVs 具有抗癌與抗發炎應用的潛力，我們也發現薑黃的成熟差異及內容物在本研究中影響 TEVs 的生物活性。在未來，影響 TEVs 功能的關鍵因素及 CEVs 其他的生物活性需要更深入的研究探討。 | zh_TW |
| dc.description.abstract | Plant-derived extracellular vesicles (PDEVs) are kind of extracellular vesicles isolated from plants, which present the various bioactivities. Turmeric is the rhizomes of Curcuma longa which is commonly used in cuisine and herbs with various bioactivities, including anti-inflammatory, anticancer, etc. However, there is little research on the turmeric derived extracellular vehicles (TEVs). Citrus fruits are commonly found in the daily diet, and are known to be rich in vitamin C and have antioxidant and anti-inflammatory properties. Citrus derived extracellular vesicles (CEVs) have been successfully isolated, but it is not clear whether there are differences between the extracellular vesicles isolated from different citrus species. Therefore, in this study, we aimed to isolate EVs from turmeric and different varieties of citrus, and analyzed their characteristics and biological functions. The TEVs were successfully isolated by tangential flow filtration (TFF), and the CEVs were purified by ultra-centrifugation and sucrose gradient centrifugation. Particle size distribution and zeta potential were subsequently measured to understand the difference between different extracellular vesicles. Moreover, small-sized RNAs were found in TEVs, and TEVs from different maturity have differences in curcumin content. In cell experiments, TEVs could inhibit proliferation of colorectal cancer cells, but CEVs don’t have same effects. Interestingly, we noted that TEVs isolated from mature turmeric couldn’t suppress the growth of cancer cells. In addition, mature TEVs were able to suppress NO production after LPS induction without killing macrophages, but these phenomena disappeared when TEVs were remanufacture as vectors. These results concluded that the TEVs have the potential ability to apply in anticancer and anti-inflammatory, and we also found that different maturity and contents of TEVs affected the biological activity in this study. In the future, we would elucidate the key factors that contribute to the functions of TEVs, and the other biological functions of CEVs need more investigation. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-06-20T16:07:01Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2023-06-20T16:07:01Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 目錄
口試委員會審定書 I 謝誌 II 中文摘要 III Abstract IV 目錄 V 圖目錄 VII 表目錄 IX 縮寫表 X 第壹章、文獻回顧 1 第一節 細胞外囊泡 (Extracellular vesicles) 1 (一) 細胞外囊泡 (Extracellular vesicles) 簡介 1 (二) 外泌體 (Exosome) 2 (三) 微囊泡 (Microvesicles) 4 (四) 凋亡小體 (Apoptotic bodies) 4 第二節 植物來源胞外囊泡 (Plant-derived extracellular vesicles) 6 (一) 植物來源性胞外囊泡之簡介 6 (二) 植物來源性胞外囊泡的分離方法 6 (三) 植物來源性胞外囊泡之生物活性 7 第三節 薑黃 (Turmeric) 10 (一) 簡介 10 (二) 薑黃之生物活性 10 第四節 柑橘類 (Citrus) 11 (一) 柑橘類簡介 11 (二) 柑橘類之生物活性 11 第貳章、研究動機與實驗架構 12 第參章、實驗材料與方法 13 第一節 實驗材料 13 （一） 實驗儀器 13 （二） 樣品與試劑 14 （一） 薑黃胞外囊泡樣品之分離 15 （二） 柳橙果肉胞外囊泡樣品之分離 16 （三） 自薑黃胞外囊泡製備奈米載體 17 （四） Curcuminoids 之萃取與分析 17 （五） 胞外囊泡物理性質測定 18 （六） 細胞培養 19 （七） 細胞存活率試驗 21 （八） 細胞培養液中一氧化氮之測定 22 （九） 蛋白質電泳 23 （十） 蛋白質銀染色法 25 （十一） RNA萃取 26 （十二） RNA電泳 27 第三節 統計分析 28 第肆章、結果與討論 29 第一節、 薑黃來源性胞外囊泡的分離與性質 29 (一) 不同成熟度的薑黃差異 29 (二) 不同成熟度的薑黃來源性胞外囊泡 31 (三) 薑黃來源性胞外囊泡的奈米性質 32 (四) 薑黃胞外囊泡的內容物 36 第二節、 柳橙來源性胞外囊泡的分離與性質 42 (一) 柳橙來源性胞外囊泡的製備與外觀 42 (二) 柳橙來源性胞外囊泡的奈米性質 44 第三節、 植物來源性胞外囊泡的生物活性 47 (一) 薑黃來源性胞外囊泡對大腸癌細胞株存活率之影響 47 (二) 柳橙果肉來源性胞外囊泡對大腸癌細胞株存活率之影響 50 (三) 成熟薑黃來源性胞外囊泡與再製載體對 RAW264.7 巨噬細胞株之影響 52 第伍章、結論 56 參考文獻 57 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | 柳橙來源胞外囊泡 | zh_TW |
| dc.subject | 抗發炎 | zh_TW |
| dc.subject | 抑制細胞增生 | zh_TW |
| dc.subject | 薑黃來源胞外囊泡 | zh_TW |
| dc.subject | citrus-derived extracellular vesicles | en |
| dc.subject | inhibition of cell proliferation | en |
| dc.subject | anti-inflammatory | en |
| dc.subject | turmeric-derived extracellular vesicles | en |
| dc.title | 薑黃與柳橙胞外囊泡特性與其細胞生物活性之研究 | zh_TW |
| dc.title | Characterization of turmeric and citrus-derived extracellular vesicles and investigation of biological activities in cell models | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 111-1 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 何元順;黃步敏;郭靜娟;王應然 | zh_TW |
| dc.contributor.oralexamcommittee | Yuan-Soon Ho;Bu-Miin Huang;Ching-Chuan Kuo;Ying-Jan Wang | en |
| dc.subject.keyword | 薑黃來源胞外囊泡,柳橙來源胞外囊泡,抑制細胞增生,抗發炎, | zh_TW |
| dc.subject.keyword | turmeric-derived extracellular vesicles,citrus-derived extracellular vesicles,inhibition of cell proliferation,anti-inflammatory, | en |
| dc.relation.page | 71 | - |
| dc.identifier.doi | 10.6342/NTU202300038 | - |
| dc.rights.note | 同意授權(限校園內公開) | - |
| dc.date.accepted | 2023-02-16 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 食品科技研究所 | - |
| dc.date.embargo-lift | 2028-01-07 | - |
| 顯示於系所單位： | 食品科技研究所 | |
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