光學同調斷層掃描術用於耳鼻喉科應用之可行性研究

Abstract

在本篇論文中第一部分，我們使用了掃頻式同調斷層掃描術(Swept-Source Optical Coherence Tomography, SS-OCT)進行小鼠內耳耳蝸之成像以及光學特性分析。首先我們架設了SS-OCT的軟硬體，軟體部分採用了C++的程式語言來進行儀器的操作以及資料擷取並且利用MFC設計出我們的操作介面。硬體部分分別利用了中心波長1.06微米與1.3微米的掃頻式雷射架設了兩套SS-OCT來進行樣本的成像與量化分析。第二部分則是我們使用了中心波長1.03微米的高折射率差光柵垂直共振腔面射型雷射(High Contrast Grating Vertical Cavity Surface Emitting Laser, HCG-VCSEL)作為SS-OCT系統的光源以達到成像使用。這是首次利用此HCG-VCSEL於SS-OCT的應用，此光源提供了等效500 kHz的掃頻速率並在此速速度下有著 48 nm的掃描頻寬，在硬體部份由於此HCG-VCSEL屬於原型機並無校準時脈訊號(k-clock)，因此我們架設了邁克生干涉儀(Michelson Interferometer) 作為校準訊號，再結合原先所架設之SS-OCT系統，我們進行了手指皮膚以及老鼠血管造影成像(Optical Coherence Tomography Angiography, OCTA)的資料擷取與成像。透過此方式我們成功的架設首套HCG-VCSEL SS-OCT並且量測其偏振特性(Uniformity of the Polarization)、靈敏度(Sensitivity)、滾降雜訊比(Roll-off, R-number)以及系統成像解析度，上述成果呈現相當完善且優異的數據資料以及成像結果。

In the first part of this dissertation, we used Swept-Source Optical Coherence Tomography (SS-OCT) to acquire image and analyze the optical properties of the mouse cochlea. First of all, we set up the software and hardware of SS-OCT. The software part adopts C++ programming language to control the instrument and acquire data and use MFC library to design our user graphic interface (GUI). In the hardware part, two SS-OCT systems were built up using wavelength-swept lasers with center wavelengths of 1.06 μm and 1.3 μm, respectively, for imaging and quantitative analysis of samples. The second part is that we use a High Contrast Grating Vertical Cavity Surface Emitting Laser (HCG-VCSEL) with a center wavelength of 1.03 μm as a light source of the SS-OCT system. This is the first time utilizing a HCG-VCSEL in SS-OCT. This HCG-VCSEL provides an effective swept rate of 500 kHz and a tuning range 48 nm. Due to the HCG-VCSEL is a prototype and has no calibrated clock signal (k-clock), we set up a Michelson Interferometer, which provides a calibration signal for wavelength calibration, then combined it with the SS-OCT system previously developed. With this dual-channel SS-OCT, we acquired the OCT imaging of fingernail junction and mouse ear OCT angiography (OCTA) imaging. By this method, we successfully built the first HCG-VCSEL based SS-OCT and measured its uniformity of the polarization, sensitivity, roll-off, R-number, and demonstration of OCT and OCTA imaging. The above results present fairly complete analysis data for system performance and excellent imaging results.