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標題: | 氮源濃度影響水稻與印度梨形孢真菌共生之探討 Effect of Nitrogen Rates on the Symbiosis between Rice and Piriformospora indica |
作者: | 陳耀堂 Yao-Tang Chen |
指導教授: | 王淑珍 Shu-Jen Wang |
關鍵字: | 水稻,氮,印度梨形孢真菌,共生效率, rice,nitrogen,Piriformospora indica,colonization efficiency, |
出版年 : | 2023 |
學位: | 碩士 |
摘要: | 土壤益生真菌的應用能在減少化學肥料的施用同時促進作物的生長,然而土壤益生真菌與作物的交互作用會受到環境因子的影響。氮為影響植物的生長發育的重要環境營養元素。印度梨形孢真菌 (Piriformosopra indica, P. indica) 是一種極具生物性肥料發展性的土壤益生真菌,其會與宿主植物的根部共生並且促進植株生長。本研究探討於氮濃度減少情況下水稻與P. indica的共生程度變化,進而了解其對水稻植株生長勢之影響,並以轉錄體分析探討氮源濃度影響P. indica與水稻共生之機制。本研究結果顯示,在氮濃度的減少下,P. indica的共生效率上升,同時在不同氮濃度接種P. indica後水稻的生長勢都有所提升。不同的水稻品種 (臺中在來一號,TCN1與臺農六十七號,TNG67) 受氮源影響的共生程度變化趨勢相近,而接種P. indica後對TCN1植株生長勢之促進效果較TNG67高,並且觀察到在較高共生效率的處理組其植株生長促進率較高。本研究觀察在共培養前期的缺氮顯著影響到水稻與P. indica的定殖百分率,由RNA-seq分析結果顯示在缺氮下接種P. indica,水稻根部部分木質素累積及許多次級代謝物途徑相關基因表現下降,推測次級代謝物或其相關植物防禦途徑為調控水稻與P. indica相互作用之重要機制。此外,germin-like protein相關基因群在正常氮與缺氮下接種P. indica表現上升,其表現可能影響水稻與P. indica共生。 Applying beneficial endophytic fungi can promote crop growth and reduce chemical fertilizer use. However, the symbiosis between beneficial endophytic fungi and crops would be affected by environmental nutrients. Nitrogen plays an important role in plant growth and development. Piriformospora indica (P. indica) is a root endophytic fungus that would be potentially developed to be a useful biofertilizer. In this research, the effects of nitrogen dosage in culture solutions on the symbiosis between rice and P. indica were observed, and the related mechanisms were discussed based on transcriptome analysis. The result showed that colonization efficiency between P. indica and rice increased when nitrogen dosage of the culture solution was reduced. The promotion rate of rice growth was also increased after P. indica inoculation under low nitrogen conditions. The trend of colonization efficiency changes of TCN1 and TNG67 rice varieties affected by nitrogen dosages were similar, but the P. indica-induced growth promotion of TCN1 rice seedings was higher than that of TNG67. Moreover, the P. indica-induced growth promotion was higher in seedings with higher P. indica colonization efficiency. Furthermore, the P. indica sporulation rate could be affected when nitrogen deficiency was treated at the co-culture stage. Transcriptome analysis showed that several genes involved in lignin accumulation and secondary metabolism were down-regulated in P. indica-inoculated plants in both sample groups with or without nitrogen, and the fold change of these genes in nitrogen deficiency conditions were higher than that in regular nitrogen treatments. It was suggested that nitrogen deficiency-promoted P. indica colonization could be mediated the regulations of secondary metabolism and defense-related pathway. Germin-like protein genes expression increased in P. indica-inoculated plants in both groups with or without nitrogen, and it was suggested to involve the regulating mechanism of P.indica-rice colnization. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/87340 |
DOI: | 10.6342/NTU202300400 |
全文授權: | 未授權 |
顯示於系所單位: | 農藝學系 |
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