建立體外人工齲齒模型用於微伸拉強度測試

Abstract

目前用於體外牙本質模擬齲齒去礦化牙本質進行牙本質黏著劑的黏著強度測試的模型可分為兩類：利用致齲細菌形成生物膜產生酸性環境，或不同酸鹼值的去礦化與再礦化循環溶液。然而，但是目前為止，尚沒有體外人工齲齒牙本質模型能製造具有類似自然牙齒牙本質小管內硬化結晶之結構成分與形態，亦即讓牙本質產生去礦化型態的受齲齒影響牙本質(caries affected dentin)，並能廣泛用於w體外試驗模擬各種真實臨床狀況。 先前實驗室配置的sclerotic dentin simulation solution可以成功地深入牙本質小管內產生仿生結晶。因此本研究目的為: 建立標準化體外人工齲齒模型，且能應用於受齲齒影響牙本質黏著測試。 本研究總共分為兩大部分:第ㄧ部分主要是建立標準化的體外人工齲齒實驗模型並進行影像觀察與硬度測試，第二部份則是利用建立出的體外人工齲齒進行牙本質黏著劑之微伸拉黏著強度測試。 結果顯示兩種人工去礦化模型皆能成功模擬自然受影響牙本質的型態，但生物膜模型表層去礦化更明顯、且影響深度更深。然而在Clearfil SE bond、G2-BOND universal、Palfique universal bond三種自酸蝕黏著劑中，自然健康牙本質之黏著強度皆顯著高於自然和人工牙本質，而自然齲齒牙本質和兩種人工牙本質三者間並無顯著差異。

At present, the common artificial carious dentin models used for in vitro dentin adhesives study can be classified into two categories: the biofilm model and the pH cycling model. Up-to-date, there is no artificial carious dentin model can mimic the natural caries affected dentin with demineralized dentin and intratubular mineral deposits. Our laboratory previously proposed a solution to produce artificial sclerotic dentin and successfully explore the crystal growth in the intratubular crystals. Therefore, we aimed to establlish an artificial caries model in vitro to simulate the caries affected dentin based on the proposed solution and compare microtensile bond strength (μ-TBS) of natural and artificial caries affected dentin. This study was carried out in two parts, Part I: to establish a standardized in vitro artificial carious dentin experimental model and carry out SEM image observation and hardness test. Part ΙΙ: To perform the μ-TBS of the dental adhesive with artificial carious dentin, natural carious dentin and sound dentin. The results show that both artificial caries affected dentin models can successfully simulate the morphology of natural affected dentin, but the biofilm model has more aggressive surface demineralization and a deeper impact. However, among the three self-etching adhesives, Clearfil SE bond, G2-BOND universal, and Palfique universal bond, the bond strength of natural healthy dentin was significantly higher than that of natural and artificial caries affected dentin. While natural caries affected dentin and two artificial caries dentin, there was no significant difference among the three.