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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/80968完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 羅翊禎(Yi-Chen Lo) | |
| dc.contributor.author | Chia-Yu Lin | en |
| dc.contributor.author | 林家羽 | zh_TW |
| dc.date.accessioned | 2022-11-24T03:24:06Z | - |
| dc.date.available | 2021-11-08 | |
| dc.date.available | 2022-11-24T03:24:06Z | - |
| dc.date.copyright | 2021-11-08 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-09-10 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/80968 | - |
| dc.description.abstract | "微生物之β-葡萄糖苷酶在不同受質之水解特異性，常應用於改善食品的風味或是提升食品中的活性成分。實驗室先前利用Kluyveromyces marxianus之 β-葡萄糖苷酶 (Bgl1)轉換苦瓜皂苷及羅漢果皂苷，發現其對於結構具有受質水解特異性，對於O-linked醣基及β-1,6特定鍵結可進行水解。人參皂苷的結構與苦瓜皂苷及羅漢果皂苷相似，因此，本研究將進一步探討Bgl1對人參皂苷之水解特性。Bgl1在30℃下，轉換原人參三醇型皂苷 (protopanaxatriol-type)，反應0.5小時，Re及Rg1濃度分別由548 ppb及80 ppb顯著降低至212 ppb及52 ppb，20(S)-Rg2及20(S)-Rh1濃度分別由170 ppb及57 ppb顯著上升至1131 ppb及212 ppb，顯示Bgl1具有能力水解人參皂苷結構中O-linked醣基；在原人參二醇型皂苷 (protopanaxadiol-type)中，Bgl1反應1小時內，Rb1濃度由2116 ppb顯著降低至13 ppb，Rd濃度由1182 ppb顯著上升至3082 ppb後，再下降至2805 ppb，並產生具有高生理活性之Compound K，顯示Bgl1可依序水解Rb1結構中β-1,6鍵結及O-linked醣基。此外，Bgl1也可水解Rc、Rb2及Rb3結構中O-linked醣基，生成稀有人參皂Compound Mc、Compound Y及Compound Mx，但皆無法水解β-1,2鍵結之葡萄糖醣基，顯示Bgl1對於四環三萜之人參皂苷具有受質水解特異性。許多文獻指出不同來源之微生物β-葡萄糖苷酶，具有不同之水解特性。本研究希望深入探討同為真菌類之Aspergillus niger，其β-葡萄糖苷酶與K. marxianus之Bgl1水解位點差異性，利用異源蛋白表現系統，使E. coli大量表現A. niger重組β-葡萄糖苷酶，然而，發現蛋白最終會以包涵體(inclusion bodies)形式生成，而未能取得水溶性蛋白，因此，無法進一步進行試驗，未來期望可藉由改變蛋白表現系統之宿主或是與其他方法繼續探討其受質水解特異性。 " | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-24T03:24:06Z (GMT). No. of bitstreams: 1 U0001-0709202118551400.pdf: 4694665 bytes, checksum: 7fda9dd94cff74fc749987c77779f526 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 目錄 第一章、前言 1 第二章、文獻回顧 2 第一節、人參 2 第二節、人參皂苷 2 一、人參皂苷結構 2 二、人參皂苷結構與生理活性之相關性 6 三、人參皂苷分析 8 第三節、人參皂苷轉換 10 一、熱加工法 10 二、化學法 10 三、生物轉換法 11 第四節、β-葡萄糖苷酶 12 一、酵母菌K. marxianus中的β-葡萄糖苷酶 12 二、黑麴菌A. niger中的β-葡萄糖苷酶 13 第三章、研究目的與實驗架構 15 第四章、材料與方法 17 第一節、實驗材料 17 一、人參樣品 17 二、菌株 17 三、質體 17 四、引子 17 五、藥品與試劑 20 六、溶劑配置 22 第二節、儀器設備 22 一、實驗耗材 22 二、一般儀器設備 23 三、套裝軟體 24 第三節、實驗方法 25 一、人參皂苷品管樣品製備 25 二、K. marxianus之Bgl1蛋白轉換人參萃取液 26 三、K. marxianus之Bgl1蛋白轉換單一人參皂苷 30 四、A.niger轉換人參皂苷及β-葡萄糖苷酶蛋白表現 35 第五章、結果與討論 39 第一節、人參皂苷萃取及分析平台之建立 39 一、品管樣品之分析條件 39 二、品管樣品之製備 40 第二節、探討K. marxianus之β-葡萄糖苷酶於人參皂苷上之水解特性 46 一、人參萃取液製備之確立 46 二、Bgl1蛋白於人參萃取液之轉換現象 46 三、Bgl1蛋白轉換單一人參皂苷 66 第三節、探討A. niger醣苷水解酶在人參皂苷之轉換特性及蛋白表現 74 一、A. niger於人參萃取物培養液之轉換能力 74 二、異源蛋白表現系統生成A. niger之β-葡萄糖苷酶 76 第六章、結論與展望 82 第七章、參考文獻 83 第八章、附錄 88 | |
| dc.language.iso | zh-TW | |
| dc.subject | 受質水解特異性 | zh_TW |
| dc.subject | β-葡萄糖苷酶 | zh_TW |
| dc.subject | O-linked醣基 | zh_TW |
| dc.subject | 人參皂苷 | zh_TW |
| dc.subject | ginsenosides | en |
| dc.subject | substrate specificity | en |
| dc.subject | β-O-glycosidic bond | en |
| dc.subject | β-glucosidases | en |
| dc.title | 探討真菌中β-葡萄糖苷酶於人參皂苷之受質特異性 | zh_TW |
| dc.title | Substrate specificity of fungus β-glucosidases on ginsenosides | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 潘敏雄(Hsin-Tsai Liu),呂廷璋(Chih-Yang Tseng),謝榮峯,陳宏彰,邱群惠 | |
| dc.subject.keyword | β-葡萄糖苷酶,受質水解特異性,O-linked醣基,人參皂苷, | zh_TW |
| dc.subject.keyword | β-glucosidases,substrate specificity,β-O-glycosidic bond,ginsenosides, | en |
| dc.relation.page | 104 | |
| dc.identifier.doi | 10.6342/NTU202103039 | |
| dc.rights.note | 同意授權(限校園內公開) | |
| dc.date.accepted | 2021-09-10 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 食品科技研究所 | zh_TW |
| 顯示於系所單位： | 食品科技研究所 | |
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