請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79154完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張建成(Chien-Cheng Chang) | |
| dc.contributor.author | Chao-Hsuan Liu | en |
| dc.contributor.author | 劉照瑄 | zh_TW |
| dc.date.accessioned | 2021-07-11T15:48:29Z | - |
| dc.date.available | 2023-08-31 | |
| dc.date.copyright | 2018-08-06 | |
| dc.date.issued | 2018 | |
| dc.date.submitted | 2018-08-01 | |
| dc.identifier.citation | [1] Sharma, M., R. Ying, G. Tarr, and R. Barnabas, Systematic review and meta-analysis of community and facility-based HIV testing to address linkage to care gaps in sub-Saharan Africa. Nature, 2015. 528(7580): p. S77.
[2] Ng, O.T., A.L. Chow, V.J. Lee, M.I. Chen, M.K. Win, H.H. Tan, A. Chua, and Y.S. Leo, Accuracy and user-acceptability of HIV self-testing using an oral fluid-based HIV rapid test. PLoS One, 2012. 7(9): p. e45168. [3] Teeparuksapun, K., M. Hedström, E.Y. Wong, S. Tang, I.K. Hewlett, and B. Mattiasson, Ultrasensitive detection of HIV-1 p24 antigen using nanofunctionalized surfaces in a capacitive immunosensor. Analytical chemistry, 2010. 82(20): p. 8406-8411. [4] Ma, Y., C. Ni, E.E. Dzakah, H. Wang, K. Kang, S. Tang, J. Wang, and J. Wang, Development of Monoclonal Antibodies against HIV-1 p24 Protein and Its Application in Colloidal Gold Immunochromatographic Assay for HIV-1 Detection. BioMed research international, 2016. 2016. [5] Pai, N.P., C. Vadnais, C. Denkinger, N. Engel, and M. Pai, Point-of-care testing for infectious diseases: diversity, complexity, and barriers in low-and middle-income countries. PLoS medicine, 2012. 9(9): p. e1001306. [6] Kersaudy-Kerhoas, M. and E. Sollier, Micro-scale blood plasma separation: from acoustophoresis to egg-beaters. Lab Chip, 2013. 13(17): p. 3323-46. [7] Nováková, L. and H. Vlčková, A review of current trends and advances in modern bioanalytical methods: chromatography and sample preparation. Analytica Chimica Acta, 2009. 656(1): p. 8-35. [8] Haeberle, S., T. Brenner, R. Zengerle, and J. Ducree, Centrifugal extraction of plasma from whole blood on a rotating disk. Lab Chip, 2006. 6(6): p. 776-81. [9] Mark, D., S. Haeberle, G. Roth, F. von Stetten, and R. Zengerle, Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications. Chem Soc Rev, 2010. 39(3): p. 1153-82. [10] Zhang, X.-B., Z.-Q. Wu, K. Wang, J. Zhu, J.-J. Xu, X.-H. Xia, and H.-Y. Chen, Gravitational sedimentation induced blood delamination for continuous plasma separation on a microfluidics chip. Analytical chemistry, 2012. 84(8): p. 3780-3786. [11] Wu, Z., Y. Chen, M. Wang, and A.J. Chung, Continuous inertial microparticle and blood cell separation in straight channels with local microstructures. Lab on a Chip, 2016. 16(3): p. 532-542. [12] Yang, X., O. Forouzan, T.P. Brown, and S.S. Shevkoplyas, Integrated separation of blood plasma from whole blood for microfluidic paper-based analytical devices. Lab on a Chip, 2012. 12(2): p. 274-280. [13] Vella, S.J., P. Beattie, R. Cademartiri, A. Laromaine, A.W. Martinez, S.T. Phillips, K.A. Mirica, and G.M. Whitesides, Measuring markers of liver function using a micropatterned paper device designed for blood from a fingerstick. Analytical chemistry, 2012. 84(6): p. 2883-2891. [14] Wong, A.P., M. Gupta, S.S. Shevkoplyas, and G.M. Whitesides, Egg beater as centrifuge: isolating human blood plasma from whole blood in resource-poor settings. Lab on a Chip, 2008. 8(12): p. 2032-2037. [15] Bhamla, M.S., B. Benson, C. Chai, G. Katsikis, A. Johri, and M. Prakash, Hand-powered ultralow-cost paper centrifuge. Nature Biomedical Engineering, 2017. 1(1): p. 0009. [16] Thorslund, S., O. Klett, F. Nikolajeff, K. Markides, and J. Bergquist, A hybrid poly (dimethylsiloxane) microsystem for on-chip whole blood filtration optimized for steroid screening. Biomedical microdevices, 2006. 8(1): p. 73-79. [17] Wang, S., D. Sarenac, M.H. Chen, S.-H. Huang, F.F. Giguel, D.R. Kuritzkes, and U. Demirci, Simple filter microchip for rapid separation of plasma and viruses from whole blood. International journal of nanomedicine, 2012. 7: p. 5019. [18] Taverniers, I., M. De Loose, and E. Van Bockstaele, Trends in quality in the analytical laboratory. II. Analytical method validation and quality assurance. TrAC Trends in Analytical Chemistry, 2004. 23(8): p. 535-552. [19] Patton, J.C., G.G. Sherman, A.H. Coovadia, W.S. Stevens, and T.M. Meyers, Ultrasensitive human immunodeficiency virus type 1 p24 antigen assay modified for use on dried wholeblood spots as a reliable, affordable test for infant diagnosis. Clinical and Vaccine Immunology, 2006. 13(1): p. 152-155. [20] Nakatsuma, A., M. Kaneda, H. Kodama, M. Morikawa, S. Watabe, K. Nakaishi, M. Yamashita, T. Yoshimura, T. Miura, and M. Ninomiya, Detection of HIV-1 p24 at attomole level by ultrasensitive ELISA with thio-NAD cycling. PloS one, 2015. 10(6): p. e0131319. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79154 | - |
| dc.description.abstract | 血液檢查是人體健康檢查中最重要的一項，因血液中包含了血球細胞、蛋白質、代謝物、細菌、病毒等眾多生命體及其衍生物，藉由檢視這些生理成分的種類與狀態，人體的健康狀況得以被定義。在進行血液檢查前，必須要先將血液進行血球血漿分離才能進行生理檢測，否則血液成份過度複雜，檢測結果容易受到干擾而失準。傳統上，醫療機構使用高速離心法做為血液分離的標準化步驟，利用離心機在高速旋轉下產生之離心力來分離血球及血漿；然而，在某些資源較為匱乏、落後的地區，人們可能無法負擔起離心機的價格，或甚至連基本的電力供應都沒有，無從進行血液檢查。有鑒於此，本研究提出以手動、不需電力之指尖陀螺為簡易離心平臺，進行離心參數與離心效果之探討。研究結果顯示，本指尖陀螺離心平臺能在數分鐘內完成使用採血針採血之體積的血液離心，且產率與純度分別可達30%與99%以上；而分離出來的血漿經過HIV-1 p24 紙張酵素結合免疫吸附檢測(Paper-basedEnzyme-linked Immunosorbent Assay, p-ELISA)，檢測之回收率(Recovery rate)可高達98%，代表本分離方法得到之血漿對檢測之干擾極低，印證了本平臺之應用可靠度。以上結果顯示，本平臺能大幅降低血液檢查之醫療成本與時間、保有檢測精確度，適合用於資源有限區域之醫療照護。未來，本平臺期望能將分離與檢測合而為一，使分離與檢測能一步到位，降低本平臺使用之複雜性，為資源匱乏地區的人們提供更方便、簡單、便宜之生醫檢測方法，以促進全球健康醫療資源之平均化。 | zh_TW |
| dc.description.abstract | Blood inspection is a significant item in health examination. Since blood contains blood cells, protein, metabolites, bacteria, viruses and various microorganisms, human health can be judged by considering these ingredients and their conditions. Blood plasma separation must be accomplished before blood inspection, otherwise blood cells and the complexity of blood composition will interfere detection process and thus lead to false and inconsistent results. Traditionally, the most common blood plasma separation method used in medical institutions is high-speed centrifuge, which utilizes centrifugal force generated by high rotation speed centrifuge machine to separate blood cells and plasma. However, in some resource-limited (RL) regions, people might not be able to afford centrifuge machines, or even worse, being lack of electricity and unable to run blood inspections. In view of this situation, we propose a simple, hand-powered and electricity-free fidget spinning centrifuge platform. Here we show the yield rate and purity of 30% and 99% of plasma separated in few minutes, and the highest recovery rate of plasma HIV-1 p24 Paper-based Enzyme-linked Immunosorbent Assay (p-ELISA) test is up to 98%, which proves only extremely low interferences occur in plasma detection and shows good reliability of this platform in practical use. To sum up, this platform greatly reduces the overall costs and the time of analysis and retain detection precision, which is suitable for improving medical care in RL regions. In the future, we expect to integrate separation and detection process onto one device to lower the complexity of this platform, provide the people in RL countries with more convenient and cheap biomedical detection methods, and promote the equalization of global medical resources distribution. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-11T15:48:29Z (GMT). No. of bitstreams: 1 ntu-107-R05543018-1.pdf: 3174226 bytes, checksum: 3ea9a9ac7159f7a057079808a8f8aefc (MD5) Previous issue date: 2018 | en |
| dc.description.tableofcontents | 致謝 ................................................................................................................................ i
摘要 ............................................................................................................................... ii Abstract......................................................................................................................... iii 圖目錄 .......................................................................................................................... iv 表目錄 ........................................................................................................................... v 第一章：前言與文獻回顧........................................................................................... 1 1.1 第三世界醫療的重要性..................................................................................... 1 1.2 第三世界大流行的疾病：愛滋病................................................................... 1 1.3 定點照護Point-of-care..................................................................................... 2 1.4 血液檢測：傳統血球血漿分離....................................................................... 2 1.5 以微流體技術進行血球血漿分離................................................................... 3 1.5.1 微流體晶片................................................................................................. 3 1.5.2 紙張及濾血薄膜分離................................................................................. 4 1.5.3 離心法......................................................................................................... 5 1.6 本研究提出之平臺........................................................................................... 6 第二章：實驗設計與流程........................................................................................... 8 2.1 陀螺離心參數測試............................................................................................. 8 2.1.1 轉速............................................................................................................... 8 2.1.2 旋轉半徑....................................................................................................... 9 2.1.3 樣品體積..................................................................................................... 10 2.1.4 保存天數..................................................................................................... 10 2.2 血漿應用於生醫檢測....................................................................................... 11 2.2.1 p24 重組蛋白標準品測試........................................................................ 12 2.2.2 額外添加p24 至全血之分離血漿測試.................................................. 13 第三章：實驗結果與討論......................................................................................... 15 3.1 陀螺離心參數測試........................................................................................... 15 3.1.1 實驗概念、參數與分離標準介紹.............................................................. 15 3.1.2 陀螺轉速..................................................................................................... 16 3.1.3 旋轉半徑..................................................................................................... 17 3.1.4 樣品量......................................................................................................... 19 3.1.5 血液保存時間............................................................................................. 20 3.2 血漿應用於生醫檢測....................................................................................... 21 3.2.1 p24 重組蛋白標準品測試........................................................................... 22 3.2.2 p24 額外添加至血漿之測試....................................................................... 22 3.2.3 血漿回收率................................................................................................. 24 第四章：結論與展望................................................................................................. 26 參考文獻 ..................................................................................................................... 27 附錄............................................................................................................................. 30 指尖陀螺離心分離理論....................................................................................... 30 | |
| dc.language.iso | zh-TW | |
| dc.subject | 指尖陀螺 | zh_TW |
| dc.subject | 離心 | zh_TW |
| dc.subject | 生醫檢測 | zh_TW |
| dc.subject | 愛滋病 | zh_TW |
| dc.subject | 微流體 | zh_TW |
| dc.subject | biomedical detection | en |
| dc.subject | fidget spinner | en |
| dc.subject | microfluidic | en |
| dc.subject | centrifuge | en |
| dc.subject | AIDS | en |
| dc.title | 指尖陀螺應用於生醫檢測 | zh_TW |
| dc.title | Development of Fidget Spinning in Biomedical Applications | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 106-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 陳建甫(Chien-Fu Chen) | |
| dc.contributor.oralexamcommittee | 朱錦洲(Chin-Chou Chu),林真真(Jen-Jen Lin),崔博翔(Po-Hsiang Tsui),黃執中(Chih-Chung Huang) | |
| dc.subject.keyword | 離心,微流體,指尖陀螺,愛滋病,生醫檢測, | zh_TW |
| dc.subject.keyword | centrifuge,microfluidic,fidget spinner,AIDS,biomedical detection, | en |
| dc.relation.page | 31 | |
| dc.identifier.doi | 10.6342/NTU201802216 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2018-08-01 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 應用力學研究所 | zh_TW |
| dc.date.embargo-lift | 2023-08-31 | - |
| 顯示於系所單位： | 應用力學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-107-R05543018-1.pdf 未授權公開取用 | 3.1 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。