Skip navigation

DSpace JSPUI

DSpace preserves and enables easy and open access to all types of digital content including text, images, moving images, mpegs and data sets

Learn More
DSpace logo
English
中文
  • Browse
    • Communities
      & Collections
    • Publication Year
    • Author
    • Title
    • Subject
  • Search TDR
  • Rights Q&A
    • My Page
    • Receive email
      updates
    • Edit Profile
  1. NTU Theses and Dissertations Repository
  2. 生命科學院
  3. 生化科技學系
Please use this identifier to cite or link to this item: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78953
Title: B 型肝炎病毒 X 蛋白質之探討:藉由調節淋巴毒素 α 和 β 的表現以促進肝癌細胞中異位類淋巴結構的生成
Analysis of the Hepatitis B Virus X Protein: Promotion of ectopic lymphoid-like structures formation by up-regulating lymphotoxin α and β expression in human hepatoma cells
Authors: Ying-Chen Huang
黃映晨
Advisor: 蕭寧馨
Co-Advisor: 何佳安
Keyword: B 型肝炎病毒,HBV X 蛋白質,淋巴毒素,非經典型NF-κB 訊息傳遞路徑,異位類淋巴結構,
HBV,HBV X protein,Lymphotoxin,Noncanonical NF-B signaling pathway,Ectopic lymphoid-like structure,
Publication Year : 2018
Degree: 碩士
Abstract: B型肝炎病毒X蛋白質 (HBx) 是由B型肝炎病毒所編碼出來的一種多功能性蛋白質,與肝癌的形成有關。異位類淋巴結構 (ELSs) 是由B細胞和T 細胞所組成的淋巴集結體,常見於慢性發炎的部位。先前的研究指出肝細胞中的ELSs對於肝癌病患有較差的預後,再者,ELSs的形成與淋巴毒素 (LT)-β所驅動之非典型NF-κB訊息傳遞途徑的活化有關。為了瞭解HBx在晚期肝癌中形成ELSs所扮演的角色,我們將HBx基因分別利用轉染和感染的方式植入肝癌細胞株中,並以即時定量聚合酶連鎖反應和西方墨點法去鑑定LT家族與非典型NF-κB相關訊息的表達。研究結果顯示,在表現HBx的HA22T/VGH肝癌細胞株中,會增加LTα和LTβ的表現,並且透過活化非經典型NF-κB訊息傳遞路徑,促進下游效應因子─趨化因子CXCL12、CXCL13和CCL21的表達,吸引B細胞與T 細胞過來聚集。由我們的研究結果可知HBx亦具有調控ELSs形成的功能,此機制的明朗化將提供一種新型的免疫治療策略,治療由HBV誘導所形成之肝癌。
Hepatitis B virus X protein (HBx) is a multifunctional protein encoded by the hepatitis B virus that involved in hepatocarcinogenesis. Ectopic lymphoid-like structures (ELSs) are lymphoid aggregates mainly composed of B cells and T cells, frequently developed at sites of chronic inflammation. Hepatic ELSs indicates poor prognosis for hepatocellular carcinoma (HCC), which are associated with activation of the lymphotoxin (LT)-β-driven non-canonical NF-κB signaling pathway. To understand the role of HBx in ELSs formation in advanced HCC, we used HBx-transfected and HBx-infected HCC cell lines to identify the expression of LT family and non-canonical NF-κB signals by real-time PCR and western bloting. The data showed that HBx expression in HA22T/VGH cells up-regulated LTα, LTβ and their downstream effectors, the chemokines CXCL12, CXCL13 and CCL21 which are necessary for recruitment of B cells and T cells, through stimulating the non-canonical NF-κB signaling pathway. Our findings revealed that HBx also functioned as a regulator in ELSs formation, providing a possibility to develop a novel immunotherapeutic strategy for treating HBV-induced HCC.
URI: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78953
DOI: 10.6342/NTU201803694
Fulltext Rights: 有償授權
metadata.dc.date.embargo-lift: 2023-08-23
Appears in Collections:生化科技學系

Files in This Item:
File SizeFormat 
ntu-107-R05b22018-1.pdf
  Restricted Access
3.05 MBAdobe PDF
Show full item record


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

社群連結
聯絡資訊
10617臺北市大安區羅斯福路四段1號
No.1 Sec.4, Roosevelt Rd., Taipei, Taiwan, R.O.C. 106
Tel: (02)33662353
Email: ntuetds@ntu.edu.tw
意見箱
相關連結
館藏目錄
國內圖書館整合查詢 MetaCat
臺大學術典藏 NTU Scholars
臺大圖書館數位典藏館
本站聲明
© NTU Library All Rights Reserved