第七型腺苷酸環化酶於第一型輔助型T細胞之探討

Abstract

Abstract Metabolism is the basic function for cells to support its physiological needs. Altering cell metabolism programs can affect the functions and fates of cells, including adaptive and innate immune cells. Transcription factor c-Maf contributes to various of cytokine productions and immune responses. However, the role of c-Maf in metabolism remains unknown. We used an online database which provides the expression profile of c-Maf-regulating genes and classified them by KEGG catalog. We found two c-Maf-regulated candidate genes, Adcy7 and Pde7a, which regulate cyclic AMP formation and decomposition, respectively. We employed lentiviral Adcy7-shRNA knockdown (KD) approach to study the role of Adcy7 in TH1 cells. Cyclic AMP (cAMP) level was decreased in Adcy7 KD TH1 cells. IFNγ gene expression and production were reduced in Adcy7 KD TH1 cells. Otherwise, the phosphorylation level of Lck, which plays important role in T cell activation, was decreased in Adcy7 KD TH1 cells. Moreover, two activation markers CD69 and CD25 expression were reduced at 90 mins and 120 mins after re-stimulation in Adcy7 KD TH1 cells. These results indicate that Adcy7 plays positive roles for cAMP production, phosphorylation of Lck, CD69/CD25 expression and IFNγ production in TH1 cells. Key words: Immunometabolism, Adenylyl cyclase 7, Helper T cells 1. CD69/CD25 expression and IFNγ production in TH1 cells. Key words: Immunometabolism, Adenylyl cyclase 7, Helper T cells 1.