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標題: | 製備用於光熱治療之含銅葉綠素鈉的奈米凝膠 Fabrication of Sodium Copper Chloropyllin-Incorporated Nanogels for Photothermal Therapy |
作者: | Chin-Feng Hsu 徐靖峰 |
指導教授: | 蔡偉博(Wei-Bor Tsai) |
關鍵字: | 奈米凝膠,光熱治療,N-(3-二甲氨基丙基)甲基丙烯?胺,銅葉綠素鈉,內噬作用, Nanogels,Photothermal therapy,N-(3-dimethylamino) propyl methacrylamide,Sodium copper chlorophyllin,Cellular uptake, |
出版年 : | 2016 |
學位: | 碩士 |
摘要: | 奈米凝膠常應用於各種領域,例如:基因輸送、癌症之熱治療以及藥物釋放等等。由於奈米凝膠在癌組織中之高通透與滯留效應,使得它具有被應用於癌症治療之潛力。熱治療之策略為藉由提高腫瘤組織周邊之溫度達41 ~ 45°C,利用癌組織相對於正常組織對於高溫之抵抗力較為薄弱,熱治療能夠在不影響正常細胞之生理機能下對癌細胞造成傷害。多數研究亦指出具有正表面電荷之奈米凝膠之細胞內噬作用較迅速。
本研究中我們使用N-(3-二甲氨基丙基)甲基丙烯酰胺(N-3-dimethylamino propyl methacrylamide, NDPMA)製作出具有正表面電荷並含有銅葉綠素鈉(Sodium copper chlorophyllin, SCC)之奈米凝膠,並在波長532 nm的雷射光照射下能產生高熱。以MTS試驗以及Live/Dead染色的結果測試細胞毒性。 實驗結果顯示L929細胞在和含銅葉綠素鈉的奈米凝膠培養過後,在綠光雷射照射下死亡;而和沒有銅葉綠素鈉之奈米凝膠培養的細胞在雷射照射下則仍生存。 Nanogels have been used as carriers in various fields, such as gene delivery, hyperthermia cancer treatment, drug delivery and so on. Thanks to the enhanced proliferation and retention effect (EPR effect), nanogels have shown great potential in cancer therapy. The approach of thermotherapy is to rise the temperature (usually 41 ~ 45°C) near the target tumor tissues. Based on the fact that cancer cells are more vulnerable to higher temperature than normal tissues, thermotherapy would damage cancer cells while those adjacent tissues remain healthy. Several study also shows that nanogel with positively surface charge could undergo fast endocytosis. In this study, we used N-(3-dimethylamino) propyl methacrylamide as monomer and fabricated nanogel with positively charged surface containing sodium copper chlorophyllin (SCC), which can produce heat simply by exposing under green laser with 532 nm wavelength. The viability was examined via MTS assay and Live/Dead staining. The result shows that L929 cells can be killed when exposed under green laser after incubation with SCC-incorporated nanogels whereas those without SCC content nanogels still alive. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78179 |
DOI: | 10.6342/NTU201601622 |
全文授權: | 有償授權 |
顯示於系所單位: | 化學工程學系 |
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